Importance of intracellular Fe pools on growth of marine diatoms by using unialgal cultures and on the Oyashio region phytoplankton community during spring

We report on the ability for luxury Fe uptake and the potential for growth utilizing intracellular Fe pools for 4 coastal centric diatom isolates and in situ phytoplankton assemblages, mainly composed of diatoms. Iron uptake of the diatom isolates and natural phytoplankton assemblages in the Oyashio region during spring blooms were prevented by adding hydroxamate siderophore desferrioxamine B (DFB). After the addition of DFB, intracellular Fe in the diatom isolates supported 2.4–4.2 cell divisions with 1.2–2.6 Chl a doublings. The intracellular Fe was primarily used for cell generation rather than Chl a production, leading to a reduction in the Chl a cell quota in the Fe-starved cells with time. The metabolic properties of the Fe-starved cells with their cell morphologies were different among species or genera. An on-deck incubation experiment also exhibited 1.9 cell divisions and 0.81 Chl a doublings of phytoplankton after the addition of DFB, also indicating the preference of cell generation over Chl a production. A decrease in the level of cellular Chl a, a main light-harvesting pigment in Fe-starved diatoms, may become a superior survival strategy to protect the cells from high irradiance that can cause photo-oxidative damages through photosynthesis. Such relatively low-Fe with high-light conditions could often occur in surface waters of the Oyashio region from spring to summer.     

Geometry of the Nojima Fault at Nojima-Hirabayashi, Japan – II. Microstructures and their Implications for Permeability and Strength

Samples of damage-zone granodiorite and fault core from two drillholes into the active, strike-slip Nojima fault zone display microstructures and alteration features that explain their measured present-day strengths and permeabilities and provide insight on the evolution of these properties in the fault zone. The least deformed damage-zone rocks contain two sets of nearly perpendicular (60–90° angles), roughly vertical fractures that are concentrated in quartz-rich areas, with one set typically dominating over the other. With increasing intensity of deformation, which corresponds generally to increasing proximity to the core, zones of heavily fragmented rock, termed microbreccia zones, develop between prominent fractures of both sets. Granodiorite adjoining intersecting microbreccia zones in the active fault strands has been repeatedly fractured and locally brecciated, accompanied by the generation of millimeter-scale voids that are partly filled with secondary minerals. Minor shear bands overprint some of the heavily deformed areas, and small-scale shear zones form from the pairing of closely spaced shear bands. Strength and permeability measurements were made on core collected from the fault within a year after a major (Kobe) earthquake. Measured strengths of the samples decrease regularly with increasing fracturing and fragmentation, such that the gouge of the fault core and completely brecciated samples from the damage zone are the weakest. Permeability increases with increasing disruption, generally reaching a peak in heavily fractured but still more or less cohesive rock at the scale of the laboratory samples. Complete loss of cohesion, as in the gouge or the interiors of large microbreccia zones, is accompanied by a reduction of permeability by 1-2 orders of magnitude below the peak values. The core samples show abundant evidence of hydrothermal alteration and mineral precipitation. Permeability is thus expected to decrease and strength to increase somewhat in active fault strands between earthquakes, as mineral deposits progressively seal fractures and fill pore spaces.     

Newly-generated Daliangshan Fault Zone—Shortcutting on the central section of Xianshuihe-Xiaojiang fault system

The Daliangshan fault zone is the eastern branch in the central section of Xianshuihe-Xiaojiang fault system. It has been neglected for a long time, partly because of no destructive earthquake records along this fault zone. On the other hand, it is located on the remote and inaccessible plateau. So far it was excluded as part of the Xianshuihe-Xiaojiang fault system. Based on the interpretation of aerophotographs and field investigations, we document this fault zone in detail, and give an estimation of strike-slip rate about 3 mm/a in Late Quaternary together with age dating data. The results suggest that the Daliangshan fault zone is a newly-generated fault zone resulted from shortcutting in the central section of Xianshuihe-Xiaojiang fault system because of the clockwise rotation of the Southeastern Tibetan Crustal Block, which is bounded by the Xianshuihe-Xiaojiang fault system. Moreover, the shortcutting may make the Daliangshan fault zone replace the Anninghe and Zemuhe fault zones gradually, and finally, the later two fault zones will probably die out with the continuous clockwise rotation.     

Sequential updating assimilation of simulated Geosat altimeter data from mesoscale features into a two-layer quasi-geostrophic model

A sequential updating method for assimilating Geosat altimeter data into an eddyresolving, quasi-geostrophic model is examined using simulated data of mesoscale features taken from a control run solution. The upper-layer streamfunction in the model is updated by the altimeter data on satellite tracks (at 110 km intervals) at times of observations (with 17-day cycles). To evaluate data suitability, the correlation between the data and the assimilation solution just before update is compared with the correlation between the two data with a 1-cycle separation: i.e., predictability is compared with persistence. The assimilation method is tested on mesoscale features such as linear Rossby waves, unstable mesoscale meanders in a jet and dipole eddies over realistic deep ocean topography. The assimilation method is successful for reconstructing the mesoscale features that evolve gradually or extend over more than one track. Assimilation is degraded by quick evolution of smaller scale features; i.e the unstable meanders that have short wavelengths and are not well captured by the altimeter with the low cross-track resolution, and the mesoscale features, whose lower layer component receives effects of bottom topography in the data but is underestimated due to inefficient downward transfer of the surface data in the assimilation.     

Estimated respiration rate of myctophid fish from the enzyme activity of the electron-transport-system

Whole animal respiration rates (R) of myctophid fishes which migrate up to the surface at night were estimated using enzyme activities of the electron-transport-system (ETS). The fish, currently unsusceptible to laboratory experimentation, were caught at sea and stored frozen at –20°C for 14–17 days prior to enzyme assay. Supplemental tests on two tropical marine fishes (gobies and poma-centrids) showed no measurable loss of ETS activity during storage for up to 36 d at –20°C. The ETS/R ratio for gobies and pomacentrids was 1.61. Respiration rates of myctophid fishes estimated using this ETS/R ratio ranged from 17.7 to 453µl O₂ individual^–1 hr^–1 for specimens weighing 26–1101 mg wet weight atin situ temperature of 24–27°C. The relationship between the respiration rate standardized to a temperature of 20°C (R:µl O₂ individual^–1hr^–1) and wet weight (WW: mg) of myctophid fishes was expressed asR=0.790 WW^0.84 (r=0.964,n=27). This relationship does not differ appreciably from the respiration rates of other marine fishes calculated from Winberg's equation.     

Seasonal Changes in Nano/Micro-Zooplankton Herbivory and Heterotrophic Nano-Flagellates Bacterivory off Cape Esan, Southwestern Hokkaido, Japan

Seasonal changes in nano/micro-zooplankton grazing on pico-, nano- and micro-size phytoplankton and heterotrophic nano-flagellates (HNF) feeding on heterotrophic bacteria were quantified by the dilution technique in the surface layer off Cape Esan, southwestern Hokkaido, Japan. Pico- and nano-size phytoplankton were major components throughout the year except in spring when a diatom bloom was observed. Although there was little seasonal variation in bacteria and HNF biomass throughout the year, the micro-zooplankton biomass varied appreciably with a peak in spring. Nano/micro-zooplankton grazing or feeding on pico-size chl-a and bacteria were well balanced throughout the year. However, nano-size and micro-size chl-a growth were much greater than grazing in summer. Nano/micro-zooplankton ingestion of phytoplankton was greater than their ingestion of bacteria almost throughout the year, which suggests phytoplankton are more important as food sources of nano/micro-zooplankton in microbial food webs off Cape Esan than bacteria off Cape Esan. This revised version was published online in July 2006 with corrections to the Cover Date.     

Mean circulation induced over bottom topography by mesoscale variabilities in the Kuroshio Extension

Circulation could be generated over bottom topography by vertical shrinking or stretching of a water parcel, in which potential vorticity is conserved. The water parcel moves up or down over the bottom topography yielding shrinking or stretching. In addition to a prevailing current which advects the water parcel in one direction, an oscillatory motion can also induce shrinking and stretching, and circulation is consequently generated over the bottom topography, once it is averaged in time. A two-layer quasi-geostrophic model has been used to reproduce mesoscale variabilities both in and under the Kuroshio Extension around the Shatsky Rise. A combination of TOPEX and ERS altimeters provided information on an eddy field near the sea surface, while a data assimilation method was used to reconstruct the flow field below the main thermocline. Among various mesoscale processes associated with the Kuroshio, it is remarkable that topographic Rossby waves trapped over the Shatsky Rise are generated by the upper-layer mesoscale variability. A persistent anticyclonic circulation is produced on the Shatsky Rise through a water parcel moving up and down over the bottom slope, and is consistent with the observed density anomaly in the WOA94.     

Numerical modeling of currents in the Drake Passage with assimilation of the experimental data of 2003

Computation of ocean currents in the Drake Passage is carried out with variational assimilation of the data of the hydrographical section across the Drake Passage carried out on December 11–15, 2003, and other data. A stream-eddy structure of the easterly Antarctic Circumpolar Current and a westerly current on the Antarctic Slope are obtained. Water transports by the different current field components and the integral transport across the Drake Passage are estimated. The necessity of direct current measurements in the Drake Passage for a correct estimate of the transport is confirmed.     

A Box Model of Glacial-Interglacial Variability in the Japan Sea

The Japan Sea has experienced drastic changes in the last 60 ka: the surface water was colder than the present value by five degrees and extremely freshened (24 ppt) in the last glacial maximum (15 ka), and then it contained Oyashio water for a few thousand years. It is an open question whether the inflow-outflow pattern was entirely reversed, opposite to the present exchange with an inflow through Tsushima Strait and an outflow through Tsugaru Strait. A box model is employed with two boxes representing the northern and the southern half domains in the upper (300-m-thick) layer. The model is driven by atmospheric forcing and inflow through Tsushima Strait and/or Tsugaru Strait. Here, the net transport through Tsushima to Tsugaru is given in the model. A baroclinic component is added to the net transport through each strait. It is the baroclinic components that allow the upper and the lower portions to flow to the opposite directions in the straits, and hence a reversal flow becomes possible against the net transport, under the condition of an extremely freshened Japan Sea. The fresh surface layer in 1814 ka is attributable to a near-shutoff of the inflow due to the low sea level. Shortly after the near-shutoff, the baroclinic transport through Tsugaru Strait yields intrusion of the Oyashio water into the Japan Sea. Thus, it is implied that Oyashio water existed in the Japan Sea a few thousand years after the reopening of Tsugaru Strait, even though the net transport was one-way, similar to the present state.