Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

褐牙鲆(♀)、夏鲆(♂)及其杂交子一代线粒体16S rDNA序列遗传特性的初步研究

以线粒体16S rRNA基因部分片段为代表研究雌褐牙鲆Paralichthys olivaceus、雄夏鲆P. dentatus及其杂交子一代间线粒体DNA的遗传特性。母本与父本序列差异较大,属种间差异,在590个位点中有28个变异位点,而且全部为简约信息位点。使用同一对引物扩增亲本与杂交子代,在杂交子代中未检测到父本的线粒体DNA类型,11个处于两种生长阶段的杂交样本仅检测到一种单倍型,而且与母本的一个单倍型为同一种,表明褐牙鲆与夏鲆杂交线粒体DNA遵循母系遗传规律。     

EDM高程导线测量中的效率和精度问题探讨

随着全站仪测角测距精度的提高,在高程联测中,用EDM高程导线测量代替三四等水准测量,成为外业高程测量的重要方法之一。分析了影响EDM高程导线测量精度的因素,讨论了提高EDM高程导线测量精度的办法,提出了效率函数的概念,并以某测区三级GPS大地控制网高程联测数据为例,从精度和效率方面进行了计算对比分析。     

现代黄河三角洲地面沉降及其原因分析

为全面和具体地了解整个黄河三角洲的地面沉降状况,收集了现代黄河三角洲地区1956、1967、1980年1∶5万比例尺的地形图资料,利用地理信息系统软件进行数字化、建立高程数据库,生成数字高程模型。通过对不同时期数字高程进行空间运算发现,1956—1980年间黄河三角洲地区地面沉降现象普遍,沉降区年平均沉降数厘米。基于数字高程空间分析结果,探讨了诱发三角洲地面沉降的自然和人为因素。     

Optimization Mathematical Model of Pile Forces for Offshore Piled Breasting Dolphins

An optimization mathematical model of the pile forces for piled breasting dolphins in the open sea under various loading conditions is presented. The optimum layout with the well distributed pile forces and the least number of piles is achieved by the multiplier penalty function method. Several engineering cases have been calculated and compared with the result of the conventional design method. It is shown that the number of piles can be reduced at least by 10%—20% and the piles‘ bearing state is improved greatly.     

气候变化中海洋和冰冻圈的变化、影响及风险

本文系统梳理了IPCC 《气候变化中的海洋和冰冻圈特别报告》(SROCC)的主要结论,并对主要观点进行了解读。报告主要关注全球变暖背景下高山、极地、海洋和沿海地区现在和未来的变化及其对人类和生态系统的影响,以及实现气候适应发展路径的方案。在全球变暖背景下,冰冻圈大面积萎缩,冰川冰盖质量损失,积雪减少,北极海冰范围和厚度减小,多年冻土升温,全球海洋持续增温,1993年以来,海洋变暖和吸热速度增加了一倍以上。同时,海洋表面酸化加剧,海洋含氧量减少。全球平均海平面呈加速上升趋势,2006—2015年全球海平面上升速率为3.6 mm/yr,是1901—1990年的2.5倍,但存在区域差异。高山、极地和海洋的生态系统的物种组成、分布和服务功能均发生变化,并对人类社会产生了显著负面影响。极端海洋气候事件发生频率增多,强度加大。1982年以来,全球范围内海洋热浪的发生频率增加了一倍,且范围更广,持续时间更长。海平面持续上升加剧了洪涝、海水入侵、海岸侵蚀等海岸带灾害,并影响沿海生态系统。海洋及冰冻圈的变化及其影响在未来一定时期仍将持续,应对这些影响而面临的挑战,应加强基于生态系统的适应和可再生能源管理,强化海岸带地区的海平面上升综合应对,打造积极有效、可持续和具有韧性的气候变化应对方案。     

Variational iteration solving method for El Nio phenomenon atmospheric physics of nonlinear model

1 IntroductionThe El Ni’o atmospheric physics oscillation is anabnormal phenomenon involved in the tropical Pacificocean- atmosphere interactions. Studies on the El Ni(ophenomenon are very attractive (Lin et al., 2000; Linet al., 2001, 2002; Wang, 2001; Feng et al., 2001;Feng et al., 2002; Liu et al., 2002; Wei and Chen,2003; Xie et al., 2002; Zhu et al., 2002; Pu et al.,2003; Gu et al., 2004; Yu and Liu, 2004). Lin and Mo(2004), Mo and Lin (2004) and Mo et al. (2004) alsostudied a s…     

Feeding habit and seasonal variation of stomach contents of sand dabs, Pseudopleuronectes yokohamae, in the South Bohai Sea

Sand dabs, an important commercial flatfish in the Bohai Sea, has recently decreased gradually in biomass, resulting from overfishing and marine pollution. Artificial culture and multiplication of sand dabs are essential and practical in China. The present paper examines the food habitof this fish to provide detailed information for its culture and multiplication.     

Observational evidence of the upwelling off cape shioyazaki in Fukushima Prefecture,Japan

Using serial observations, evidence of the existence of upwelling in the region between the northeast flowing Kuroshio and the coast of Ibaraki and Fukushima Prefectures is presented. The complex and variable oceanic condition in this region sometimes conceals some of the evidence of upwelling. Evidence of upwelling can be seen in the distributions of temperature and salinity, and more clearly in those of dissolved oxygen and phosphate. The average position of the upwelling region is just off Cape Shioyazaki in Fukushima Prefecture. Such an upwelling phenomenon may be a general oceanic feature characteristic of the region at the poleward edge of western boundary currents.     

规则波在缓坡上变形与破碎数值分析

通过在二维数值水槽内用边界元法直接求解Laplace方程,对规则波在缓坡上的变形及破碎进行了数值计算。分析了不同底坡及采用不同底摩阻系数时规则波的破碎特征,并对规则波破碎的极限坡度进行了研究。重点分析了规则波破碎时海底坡度、底摩阻系数及波形不对称性对破碎指标的影响。