Monitoring of DSP toxins in small-sized plankton fraction of seawater collected in Mutsu Bay,Japan, by ELISA method: relation with toxin contamination of scallop

Monitorings were conducted on DSP toxins in mid-gut gland of scallop (mouse assay), cell numbers of toxic dinoflagellate species of Dinophysis, and diarrhetic shellfish poisoning (DSP) toxins in small-sized (0.7-5 microm) plankton fraction of seawater collected from surface (0 m) and 20 m depth at a station in Mutsu Bay, Aomori Prefecture, Japan, in 2000. A specific enzyme-linked immunosorbent assay (ELISA) was employed for the analysis of DSP toxins in small-sized plankton fraction using a mouse monoclonal anti-okadaic acid antibody which recognizes okadaic acid, dinophysistoxin-1, and dinophysistoxin-3. DSP toxins were detected twice in the mid-gut gland of scallops at 1.1-2.3 MU (mouse units) g(-1) on 26 June and at 0.6-1.2 MU g(-1) on 3 July, respectively. Relatively high cell densities of D. fortii were observed on 26 June and 11 September, and may only contribute to the bivalve toxicity during late June to early July. D. acuminata did not appear to be responsible for the toxicity of scallops in Mutsu Bay in 2000. ELISA monitoring of small-sized plankton fraction in seawater could detect DSP toxins two weeks before the detection of the toxin in scallops, and could do so two weeks after the loss of the bivalve toxicity by mouse assay. On 17 July, toxic D. fortii was detected at only small number, <10 cells l(-1), but DSP toxins were detected by the ELISA assay, suggesting a presence of other toxic small-sized plankton in seawater. For the purpose of reducing negative impacts of DSP occurrences, monitorings have been carried out hitherto on DSP toxins of bivalve tissues by mouse assay and on cell densities of "toxic" species of Dinophysis. Here we propose a usefulness of ELISA monitoring of plankton toxicity, especially in small-sized fraction, which are possible foods of mixotrophic Dinophysis, as a practical tool for detecting and predicting DSPs in coastal areas of fisheries grounds of bivalve aquaculture.     

Seasonal Trend in the Occurrence of Nocturnal Drainage Flow on a Forested Slope Under a Tropical Monsoon Climate

Using data for one year, we examined the vertical wind speed profileson a mountain slope covered with forest in northern Thailand undera tropical monsoon climate. We defined two profile patterns: higherwind speeds at greater heights (Pattern 1) and lower wind speeds atgreater heights (Pattern 2). We classified 9.4% of the data as Pattern 2;this pattern tended to occur during the night, at low wind speeds, and with high outgoing longwave radiation. In addition, stable stratification anddecoupling between the canopy surface air and the overlying layers wereobserved when Pattern 2 occurred frequently. These facts suggested thatPattern 2 was caused by a nocturnal drainage flow. The occurrence ofPattern 2 showed a clear seasonal trend, indicating that there is a seasonaltrend in the occurrence of nocturnal drainage flows. Pattern 2 was observedmore frequently between August and February and less frequently betweenMarch and July. This corresponded to the seasonal trend in wind speed, butdid not correspond to the seasonal trend in the outgoing longwave radiation.     

Wind- and density-driven circulation in a bay on the Sanriku ria coast,Japan: study of Shizugawa Bay facing the Pacific Ocean

Flow fields in Shizugawa Bay on the Sanriku ria coast, which faces the Pacific Ocean, were investigated using hydrographic observations for the purpose of understanding oceanographic conditions and the process of water exchanges in the bay after the 2011 earthquake off the Pacific coast of Tohoku. In spring to summer, density-driven surface outflow is part of estuarine circulation and is induced by a pressure gradient force under larger longitudinal gradients in density along with lower salinity water in the innermost part of the bay, regardless of wind forcing. In winter to summer, another density-driven current with a thermal structure is induced by a pressure gradient force under the smaller longitudinal density gradients in calm wind conditions. Particularly in winter, Tsugaru Warm Current water can be transported in the surface layer inside the bay. Wind-driven bay-scale circulation with downwind and upwind currents in the surface and deeper layers, respectively, is induced by strong longitudinal wind forcing under the smaller longitudinal density gradients, irrespective of season. Particularly in fall to spring, this circulation can cause the intrusions of oceanic water associated with Oyashio water and Tsugaru Warm Current water in the deeper layer. These results suggest that wind- and density-driven currents can produce the active exchange of water from inside and outside the bay throughout the year.     

Millennial-scale vegetation changes during the last 40,000 yr based on a pollen record from Lake Biwa, Japan

A high-resolution pollen record covering the last 40,000 yr (BIW95-4) from Lake Biwa, western Japan, shows regional vegetation responses to millennial-scale climate changes. From 40 to 30 ka, Cryptomeria japonica was dominant around the lake among pinaceous conifers and deciduous broad-leaved trees. During this period, fluctuations of C.japonica are correlated with Dansgaard-Oeschger (D-O) cycles recognized from the anhysteretic remanent magnetization (ARM) record. Increases in the abundance of this taxon may have been caused by wetter summer conditions influenced by the East Asian monsoon or increased snowfall on the Sea of Japan side of the Japanese archipelago. Between 29 and 14 ka, pinaceous conifer forests mainly composed of Pinus subgenus Haploxylon, Tsuga, and Picea trees developed. At approximately 23 ka, Picea trees increased in abundance as ARM values decreased. This expansion of Picea trees has been correlated with Heinrich event (HE) 2 in the North Atlantic. At about 14 ka, the distribution of broad-leaved forest (mainly composed of deciduous oaks) began to expand after D-O 1. Evidence of significant vegetation change related to the abrupt Younger Dryas cooling event has not been found.     

Geochemical composition of dykes along the Cameroon Line (CL): Petrogenesis and similarities with the Central Atlantic Magmatic Province

We report whole-rock geochemistry and Sr–Nd–Pb isotopic compositions of mafic dykes intruded in the Precambrian granito-gneissic basement complex, exposed at Nyos, Batibo, Dschang and Foumban on the Cameroon Line. The dykes are alkaline (Batibo), transitional (Foumban), and subalkaline (Nyos, Batibo and Dschang) with SiO₂ of 45–54 wt% and MgO of 2–9 wt%, similar to dykes reported in other areas of the Cameroon Line (CL) and the Central Atlantic Magmatic Province (CAMP). The abundances of rare earth elements (REE) and the Primitive Mantle normalised patterns for the Nyos, Batibo and Dschang dykes are similar to those of MORB, indicating that the dykes formed at shallower depths by a higher degree of partial melting relative to the Foumban dykes and the alkaline lavas of the CL. The transitional basaltic dykes with steeper REE patterns have their sources at deeper levels in the lithospheric mantle, possibly the garnet-spinel transition zone and were generated by a lower degree partial melting of the lithospheric and plume components. The Nyos and Batibo subalkaline dykes show similar isotopic compositions with a spectrum extending from depleted (DMM-like) to enriched (EM1-like) mantle, indicating the similarity in their source components. The Dschang dykes show distinct isotopic characteristics with relatively unradiogenic Nd-Pb isotope compositions compared to the Batibo and Nyos dykes. The Foumban transitional dykes with characteristic wide ranges in Sr-Nd-Pb isotopic compositions reveal varying contributions from enriched mantle components (EM1 and EM2) in addition to its plume signature similar to those of CL lavas. The Nyos and Batibo dykes alongside other dykes on the CL have low TiO₂ abundances (<2 wt%), negative PM-normalised Nb-anomalies, and moderately to strongly enriched REE patterns, and isotopic composition that overlaps with those of CAMP, suggesting a similar lithospheric origin.     

Progressive evolution of whole‐rock composition during metamorphism revealed by multivariate statistical analyses

The geochemical evolution of metamorphic rocks during subduction‐related metamorphism is described on the basis of multivariate statistical analyses. The studied data set comprises a series of mapped metamorphic rocks collected from the Sanbagawa metamorphic belt in central Shikoku, Japan, where metamorphic conditions range from the pumpellyite–actinolite to epidote–amphibolite facies. Recent progress in computational and information science provides a number of algorithms capable of revealing structures in large data sets. This study applies k‐means cluster analysis (KCA) and non‐negative matrix factorization (NMF) to a series of metapelites, which is the main lithotype of the Sanbagawa metamorphic belt. KCA describes the structures of the high‐dimensional data, while NMF provides end‐member decomposition which can be useful for evaluating the spatial distribution of continuous compositional trends. The analysed data set, derived from previously published work, contains 296 samples for which 14 elements (Si, Ti, Al, Fe, Mn, Mg, Ca, Na, K, P, Rb, Sr, Zr and Ba) have been analysed. The KCA and NMF analyses indicate five clusters and four end‐members, respectively, successfully explaining compositional variations within the data set. KCA indicates that the chemical compositions of metapelite samples from the western (Besshi) part of the sampled area differ significantly from those in the east (Asemigawa). In the west, clusters show a good correlation with the metamorphic grade. With increasing metamorphic grade, there are decreases in SiO₂ and Na₂O and increases in other components. However, the compositional change with metamorphic grade is less obvious in the eastern area. End‐member decomposition using NMF revealed that the evolutional change of whole‐rock composition, as correlated with metamorphic grade, approximates a stoichiometric increase of a garnet‐like component in the whole‐rock composition, possibly due to the precipitation of garnet and effusion of other components during progressive dehydration. Thermodynamic modelling of the evolution of the whole‐rock composition yielded the following results: (1) the whole‐rock composition at lower metamorphic grade favours the preferential crystallization of garnet under the conditions of the garnet zone, with biotite becoming stable together with garnet in higher‐grade rock compositions under the same P–T conditions; (2) with higher‐grade whole‐rock compositions, more H₂O is retained. These results provide insight into the mechanism suppressing dehydration under high‐P metamorphic conditions. This mechanism should be considered in forward modelling of the fluid cycle in subduction zones, although such a quantitative model has yet to be developed.     

Spatial variations in throughfall in a Moso bamboo forest: sampling design for the estimates of stand‐scale throughfall

We investigated the spatial and seasonal variations in throughfall (Tf) in relation to spatial and seasonal variations in canopy structure and gross rainfall (Rf) and assessed the impacts of the variations in Tf on stand‐scale Tf estimates. We observed the canopy structure expressed as the leaf area index (LAI) once a month and Tf once a week in 25 grids placed in a Moso bamboo (Phyllostachys pubescens) forest for 1 year. The mean LAI and spatial variation in LAI did have some seasonal variations. The spatial variations in Tf reduced with increasing Rf, and the relationship between the spatial variation and the Rf held throughout the year. These results indicate that the seasonal change in LAI had little impact on spatial variations in Tf, and that Rf is a critical factor determining the spatial variations in Tf at the study site. We evaluated potential errors in stand‐scale Tf estimates on the basis of measured Tf data using Monte Carlo sampling. The results showed that the error decreases greatly with increasing sample size when the sample size was less than ～8, whereas it was near stable when the sample size was 8 or more, regardless of Rf. A sample size of eight results in less than 10% error for Tf estimates based on Student's t‐value analysis and would be satisfactory for interception loss estimates when considering errors included in Rf data. Copyright © 2009 John Wiley & Sons, Ltd.     

Temporal variations of the winter mixed layer south of the Kuroshio Extension

A monthly mean time series of the temperature profile in the recirculation gyre south of the Kuroshio Extension has been produced for the period 1971–2007 to examine temporal variations of the winter mixed layer. The winter mixed layer depth (MLD) shows both interannual and decadal variations and is significantly correlated with variation of the mean net surface heat flux in late autumn to early winter. There is also a close relation with the strength of pre-existing subsurface stratification, measured as vertical temperature gradients in the preceding summer. Linear multiple regression analysis shows that a significant fraction of the variations in the winter MLD is explained by the surface heat flux and the strength of the stratification. The contribution of the two factors is comparable.     

Topography and volume effects on travel distance of surface failure

The authors investigated factors affecting the travel distance of a surface failure and developed a simple model for estimating the distance based on soil properties and topographic factor. The authors conducted field surveys and various soil tests for a number of surface failures that occurred during torrential rain at the end of August 1998 in southern Fukushima Prefecture, Japan. The authors studied the effects of various factors such as landslide volume, pore-water pressure, slope inclination, and the internal friction near the slip surfaces on the travel distances. The analyses showed no clear relationship between landslide volume and equivalent coefficient of friction, which was likely attributable to the very small range of volume compared to other studies on catastrophic landslides. The effect of excess pore-water pressure was likely negligible because undrained conditions were not to be maintained at the shallow flow depths. A positive correlation was shown between slope inclination and equivalent coefficient of friction. This correlation was attributable to two factors, one was positive correlation between the internal friction on the slip surface and slope inclination, and the other was the kinetic energy dissipation of moving mass that occurred at the inclination changing point between a slope and a sedimentary flat surface. The authors then developed a predictive model for critical flow inclination of landslide by installing the factors of soil properties and slope inclination factors. The model predicted equivalent coefficient of frictions, which were very similar to the observed values, thus verifying the effectiveness of the model.