岩藻黄素纳米复合物的制备及抗氧化、抗肿瘤活性研究

岩藻黄素是一种脂溶性类胡萝卜素,由于其水溶性差、环境敏感性强,使其应用受到限制。本研究采用有机溶剂浸提、大孔树脂纯化法,从裙带菜中提取高纯度岩藻黄素。通过复凝聚反应制备乳清分离蛋白-岩藻黄素-阿拉伯胶纳米复合物。通过透射电镜观察,纳米复合物呈光滑的球形,粒径约为44nm。红外光谱则表明,岩藻黄素与乳清分离蛋白、阿拉伯树胶发生相互作用,导致联烯键、乙酰基等特征吸收峰被遮蔽。与单一岩藻黄素相比,纳米包封的岩藻黄素具有较好的抗氧化活性稳定性。抗肿瘤实验表明,纳米复合物保留了岩藻黄素抑制HT29细胞增殖的生物活性。     

不同产地浒苔对幼刺参生长、消化和非特异性免疫的影响

为探究不同产地浒苔型饵料对幼刺参生长、消化和非特异性免疫的影响,本实验将青岛浒苔与宁波浒苔的干粉与海泥分别按一定质量比例混合,开展刺参饲喂实验,并与刺参天然饵料马尾藻进行对比。结果表明,青岛浒苔饵料和马尾藻饵料喂养的刺参的粗蛋白含量分别为14.31%±0.10%和15.43%±1.41%,显著高于宁波浒苔饵料(11.17%±0.63%),粗脂肪和灰分含量无显著差异;青岛浒苔组、宁波浒苔组和马尾藻组的增重率分别为22.65%±5.68%、3.03%±1.17%和20.47%±2.01%,特定生长率分别为(1.44±0.33)、(0.21±0.08)、(1.33±0.12)%/d,青岛浒苔组和马尾藻组刺参的增重率和特定生长率显著高于宁波浒苔组;青岛浒苔组、宁波浒苔组和马尾藻组刺参肠道淀粉酶、胃蛋白酶、胰蛋白酶和纤维素酶活力无显著差异;马尾藻组刺参体腔液碱性磷酸酶活力为(17.57±4.56)金氏单位/100mL,显著高于青岛浒苔组[(5.56±1.32)金氏单位/100mL]和宁波浒苔组[(2.83±0.75)金氏单位/100mL],超氧化物歧化酶和酸性磷酸酶活力无显著差异。由此可见,绿潮暴发时,通过打捞浒苔用以配制刺参饵料,既有助于缓解绿潮的生态灾害,又能够补充刺参饵料来源,具有广阔的生态效益和市场前景,但是其营养成分影响因素较为复杂,配制饵料时应充分考虑不同品种、采集时间和生长地点的差异,并通过一些前处理手段充分发挥浒苔的饵料价值。     

基于纹理特征的围填海SAR图像分水岭分割方法

本文提出了一种基于纹理特征的围填海SAR图像分水岭分割方法,首先对机载MiniSAR图像进行灰度共生矩阵纹理滤波,获得纹理特征图像,再对纹理特征图像进行分水岭算法分割,将获得的形态学重建图像进行门限阈值分割,得到最后的二值化分割结果。该方法一方面通过调整灰度共生矩阵纹理滤波的窗口大小,抑制了斑点噪声的影响;另一方面,利用分水岭算法对边缘模糊杂乱图像的优势,提高了围填海信息提取的准确性。实验结果表明,本方法对高分辨率SAR图像围填海监测图像的分割效果良好。     

玉米素和水杨酸对雨生红球藻(Haematococcus pluvialis)生长及虾青素积累的影响

虾青素是一种具有强抗氧化活性的类胡萝卜素,而雨生红球藻是天然虾青素的主要来源。本文以雨生红球藻(Haematococcus pluvialis)为材料,研究了植物激素玉米素和水杨酸对雨生红球藻的生长、虾青素含量及相关基因表达的影响。分别添加5种浓度的玉米素或水杨酸,结果发现0.05mg/L玉米素或25mg/L水杨酸处理5d后雨生红球藻虾青素积累最多。该浓度玉米素或水杨酸可显著提高光胁迫下藻细胞密度,最高分别达到3.4×10~5cell/mL和3.0×10~5cell/mL;同时玉米素与水杨酸组中虾青素含量显著上升,分别为1.7%和1.6%,比对照组分别增加29.2%和25.6%。玉米素缓解了高光逆境条件下光合作用基因——Rubisco大亚基(rbcL)及其活化酶(rca)、碳酸酐酶(ca)的下调表达,但对虾青素合成途径β-胡萝卜素酮化酶基因(bkt)的表达量没有显著影响;而水杨酸则相反,在胁迫后期不能缓解光合作用相关基因的下调表达,但可使bkt基因显著上调,最高可达对照组的2.5倍。本研究首次比较了玉米素和水杨酸对雨生红球藻生长和虾青素积累的影响,发现玉米素比水杨酸具有更好的促进雨生红球藻中虾青素积累的效果。     

300 BC-1900 AD无定河流域城镇时空格局演变

通过分析和整理从战国中晚期（约300 BC）至清末（约1900 AD）无定河流域历代县级及以上城址的位置、兴废年代数据,结合行政区划沿革、经济社会发展、政权更迭等资料,分析流域城镇格局的时空演变过程。研究表明：① 受气候周期性波动影响,无定河流域城镇的兴起与衰废具有明显的周期性特征,城镇几何中心的移动轨迹具有明显的“西北—东南”向潮汐性运动特征;② 城址存续年限普遍较短,具有明显的阶段性特征,流域内曾存在过4个阶段性中心城镇,中心城镇移动的方向和过程与城镇几何中心的潮汐性移动过程趋势一致;③ 流域城镇空间格局可分为3种类型,即秦汉与隋唐时期的沿河流谷地分布,宋、明两代的沿边境线与长城分布,以及元、清两代集中于流域下游分布。流域城镇未来的发展布局应重视气候变化对城镇分布的长期影响,关注区域环境的脆弱性,合理安排城镇体系发展规模与布局。     

京津冀协同发展重要地理国情监测研究与应用

文章阐述了开展京津冀协同发展重要地理国情监测的现实背景和意义,依托地理国情普查和常态化监测,结合京津冀一体化发展需求,提出了京津冀协同发展重要地理国情监测的总体思路、研究内容、关键技术和研究方法等,并开展了京津冀地区自然生态空间变化监测、高等级公路和铁路交通网络变化监测、重点大气颗粒物污染源空间分布监测等重要地理国情信息监测研究,成果已经在京津冀地区可持续发展评价、京津冀生态文明建设、空间规划管理等工作中得到了广泛应用。     

Smectite formation in metalliferous sediments near the East Pacific Rise at 13°N

A 43 cm long E271 sediment core collected near the East Pacific Rise(EPR) at 13°N were studied to investigate the origin of smectite for understanding better the geochemical behavior of hydrothermal material after deposition.E271 sediments are typical metalliferous sediments. After removal of organic matter, carbonate, biogenic opal,and Fe-Mn oxide by a series of chemical procedures, clay minerals(2 μm) were investigated by X-ray diffraction,chemical analysis and Si isotope analysis. Due to the influence of seafloor hydrothermal activity and close to continent, the sources of clay minerals are complex. Illite, chlorite and kaolinite are suggested to be transported from either North or Central America by rivers or winds, but smectite is authigenic. It is enriched in iron, and its contents are highest in clay minerals. Data show that smectite is most likely formed by the reaction of hydrothermal Fe-oxyhydroxide with silica and seawater in metalliferous sediments. The Si that participates in this reaction may be derived from siliceous microfossils(diatoms or radiolarians), hydrothermal fluids, or detrital mineral phases. And their δ30 Si values are higher than those of authigenic smectites, which implies that a Si isotope fractionation occurs during the formation because of the selective absorption of light Si isotopes onto Feoxyhydroxides. Sm/Fe mass ratios(a proxy for overall REE/Fe ratio) in E271 clay minerals are lower than those in metalliferous sediments, as well as distal hydrothermal plume particles and terrigenous clay minerals. This result suggests that some REE are lost during the smectite formation, perhaps because their large ionic radii of REE scavenged by Fe-oxyhydroxides preclude substitution in either tetrahedral or octahedral lattice sites of this mineral structure, which decreases the value of metalliferous sediments as a potential resource for REE.     

岛屿海岸线自动化提取及其时空变迁研究——以大连市獐子岛为例

海岸带是受人类活动和全球海平面上升影响的敏感地带,海岸线的提取和监测是海岸带生态系统研究和社会管理的重要内容。本文在遥感和地理信息系统的支持下,以修正的归一化水体指数(Modified Normalized Difference Water Index,MNDWI)为基础,结合遥感影像处理和直方图均衡化等技术,实现了大连市獐子岛1985—2016年海岸线的自动化提取。结果表明:(1)通过与三位专家目视解译的成果比对,本文提取海岸线的精度能满足后续研究的要求(相对误差分别为0.045%,0.032%和0.023%);(2)近30年来,獐子岛海岸线总体呈现蚀退趋势,岸线长度与岛屿面积分别呈现变短和变小的趋势,獐子岛(主岛)和大耗岛的岸线蚀退速率最大,褡裢岛次之,小耗岛最小;在人类活动较为密集的区域,海岸线呈现出较为强烈的增长趋势,海水养殖和圈海建坝是岸线增长的主要驱动力;(3)獐子岛海岸线具有显著的分形性质,分形维数随时间呈现增大的趋势,獐子岛(主岛)的分形维数最大,褡裢岛的分形维数最小。     

基于高斯烟羽模型的船舶尾气扩散研究

为有效对港区大气污染进行治理、分析船舶尾气,本文详细介绍了一种基于高斯烟羽模型,通过MATLAB模拟仿真模型,其包括实验仿真过程、技术原理及理论模型对船舶尾气扩散进行的研究。该模型是在传统的高斯烟羽模型的基础上,通过对实源像源进行加权选择输入参数;通过矢量合成确定了气体扩散的方向,利用合成后的"风速"进行计算仿真,有效模拟了船舶尾气在港区或者海洋环境中的气体扩散模型。其模型简单且可以有效模拟船舶尾气扩散。并且进一步对后续模型的精确优化进行分析。     

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.