Sulfur and oxygen isotope study of sulfate reduction in experiments with natural populations from Fællestrand, Denmark

This study investigates the sulfur and oxygen isotope fractionations of dissimilatory sulfate reduction and works to reconcile the relationships between the oxygen and sulfur isotopic and elemental systems. We report results of experiments with natural populations of sulfate-reducing bacteria using sediment and seawater from a marine lagoon at Fællestrand on the northern shore of the island of Fyn, Denmark. The experiments yielded relatively large magnitude sulfur isotope fractionations for dissimilatory sulfate reduction (up to approximately 45‰ for ³⁴S/³²S) with higher δ¹⁸O accompanying higher δ³⁴S, similar to that observed in previous studies. The seawater used in the experiments was spiked by addition of ¹⁷O-labeled water and the ¹⁷O content of residual sulfate was found to depend on the fraction of sulfate reduced in the experiments. The ¹⁷O data provides evidence for recycling of sulfur from metabolic intermediates and for an ¹⁸O/¹⁶O fractionation of ∼25-30‰ for dissimilatory sulfate reduction. The close correlation between the ¹⁷O data and the sulfur isotope data suggests that isotopic exchange between cell water and external water (reactor water) was rapid under experimental conditions. The molar ratio of oxygen exchange to sulfate reduction was found to be about 2.5. This value is slightly lower than observed in studies of natural ecosystems [e.g., Wortmann U. G., Chernyavsky B., Bernasconi S. M., Brunner B., Böttcher M. E. and Swart P. K. (2007) Oxygen isotope biogeochemistry of pore water sulfate in the deep biosphere: dominance of isotope exchange reactions with ambient water during microbial sulfate reduction (ODP Site 1130). Geochim. Cosmochim. Acta71, 4221-4232]. Using recent models of sulfur isotope fractionations we find that our combined sulfur and oxygen isotopic data places constraints on the proportion of sulfate recycled to the medium (78-96%), the proportion of sulfur intermediate sulfite that was recycled by way of APS to sulfate and released back to the external sulfate pool (∼70%), and also that a fraction of the sulfur intermediates between sulfite and sulfide were recycled to sulfate. These parameters can be constrained because of the independent information provided by δ¹⁸O, δ³⁴S, δ¹⁷O labels, and Δ³³S.     

宜兴山区采矿业可持续发展的问题与对策

采矿业已经而且还将继续在宜兴山区经济发展中发挥举足轻重的作用，但目前 对矿产资源的开发利用方式将严重阻碍宜兴市山区采矿业的可持续发展，在分析其存在的主 要问题的基础上提出了一些积极的对策．     

Requirements for large-eddy simulation of surface wind gusts in a mountain valley

During the passage of a front, data from a light-weight cup anemometer and wind vane, sited in a steep-walled glacial valley of the Mt Cook region of the Southern Alps of New Zealand, were analysed to derive a power spectrum of the wind velocity for periods between 0.5 and 16 min. The energy spectrum roughly followed a -5/3 power law over the range of periods from 0.5–4 min — as might be expected in the case of an inertial subrange of eddies. However, any inertial subrange clearly does not extend to periods longer than this. We suggest that the observed eddies were generated in a turbulent wake associated with flow separation at the ridge crests, and large eddies are shed at periods of 4–8 min or more.A compressible fluid-dynamic model, with a Smagorinsky turbulence closure scheme and a law of the wall at the surface, was used to calculate flow over a cross section through this area in neutrally stratified conditions. A range of parameters was explored to assess some of the requirements for simulating surface wind gusts in mountainous terrain in New Zealand.In order to approximate the observed wind spectrum at Tasman aerodrome, Mount Cook, we found the model must be three-dimensional, with a horizontal resolution better than 250 m and with a Reynolds-stress eddy viscosity of less than 5 m² s^-1. In two-dimensional simulations, the eddies were too big in size and in amplitude and at the surface this was associated with reversed flow extending too far downstream. In contrast the three-dimensional simulations gave a realistic gusting effect associated with large scale cat's paws (a bigger variety of those commonly seen over water downstream of moderate hills), with reversed flow only at the steep part of the lee slope. The simulations were uniformly improved by better resolution, at all tested resolutions down to 250 m mesh size.The spectra of large eddies simulated in steep terrain were not very sensitive to the details of the eddy stress formulation. We suggest that this is because boundary-layer separation is forced in any case by terrain-induced pressure gradients.     

The Effect of an Infaunal Suspension Feeding Bivalve Mercenaria mercenaria (L.) on Benthic Recruitment*

Abstract. Animal-animal interactions are recognized as critical in benthic invertebrate recruitment. The hypothesis tested was that benthic recruitment is more successful in low densities of infaunal suspension feeders than in high densities. Densities of young hard clams Mercenaria mercenaria (L.) ranging from 82.5 to 330m^-2 were placed in defaunated boxes of sand. The mean number of species, mean number of individuals, mean wet weight biomass, mean species richness and mean dominance index per sample were calculated per density of hard clams from May to October. The experiment did not support the hypothesis. It was concluded that feeding behaviour of dense populations of M. mercenaria did not preclude successful recruitment of other benthic species.