Textural evidences for dissolution of silica-rich rocks of the Ypresian phosphatic series,Gafsa-Metlaoui basin,southwestern Tunisia: implication of biogenic silica supply on genesis of fibrous clays

The present work aimed to determine the mineralogical composition of Ypresian series and to clarify the influence of the dissolution of siliceous frustules on the genesis of fibrous clay minerals. The specimens sampled from CPG trench are mainly constituted of silica-rich rocks at Mides area located at the western part of Gafsa-Metlaoui basin. The samples were investigated using X-ray diffraction (XRD) and scanning electron microscopy (SEM) in order to determine texture of constituents. The data obtained indicate that the bulk rock samples are mainly made up of opal CT and clay minerals. The latter consists of palygorskite-sepiolite minerals associated with smectite and few amount of illite. The trend of mineralogical composition indicates that fibrous clay minerals are more concentrated at the upper part. SEM observations indicated that palygorskite mineral appears as thread-like facies, which surround foliated texture of smectite in the lower part of the Mides section, although with the low Mg activity confirmed by the absence of dolomite. But, at the upper part of the Mides section, SEM observations revealed the occurrence of siliceous frustules, which have numerous dissolved areas and replacement of carbonate tests by silica. The dissolution saturated the depositional environment with silica which is required for the formation of palygorskite and sepiolite minerals, in addition to high Mg activity confirmed by the presence of dolomite in the bulk rock, which is required basically for the formation of sepiolite. Although the genesis mode of palygorskite and sepiolite is similar with very little difference, the genesis of sepiolite needs a high alkalinity than the formation of palygorskite.     

海洋Pseudomonasaeruginosasp.DES-3的鉴定以及基因ap02对其产蛋白酶的影响研究

海洋是地球上最大的生态系统,其多样独特的生态环境造就了其有别于其他环境的微生物资源。本研究利用蛋白酶的活性筛选策略,从辽宁省营口市某海洋淤泥中分离得到一株产蛋白酶的优势菌株DES-3。通过微生物形态学特征、生理生化性质和16SrDNA基因鉴定以及系统发育进化关系的分析,将该菌株鉴定为铜绿假单胞菌属,命名为Pseudomonas aeruginosa sp.DES-3。其胞外蛋白酶的最适反应温度和最适pH分别为55℃和9.0。将来自于碱性污染土壤宏基因组文库的编码碱性蛋白酶基因ap02与广宿主表达载体pBBR1MCS-5连接,转化至野生型菌株P.aeruginosasp.DES-3细胞中,成功构建了基因工程菌株P.aeruginosasp.DES-3/pBBR1MCS-5-ap02。基因工程菌株的最适反应温度为60℃,较野生型菌株提高了5℃。本研究构建基因工程菌株的策略可为菌株的改造和相应工业应用提供一定的技术参考。     

天津滨海新区抽水引起地面沉降现场试验研究

在天津滨海新区中新生态城服务中心开展了承压含水层抽水引起地面沉降的现场试验,采用振弦式孔隙水压力传感器和一孔多标数据采集器实时监测孔隙水压力和分层标的变化,分析了土体的变形性质和分层沉降的规律。试验结果表明,黏土层变形明显滞后于承压含水层水位的变化,以塑性变形为主且存在蠕变现象,而砂层既存在弹性变形,也存在一定的塑性变形和蠕变性。短期抽取地下水会使抽水井附近承压含水层上覆土体出现上小下大的沉降规律,且最大沉降出现在有明显孔隙水压力变化的土层顶板位置。     

Interior boundary-aligned unstructured grid generation and cell-centered versus vertex-centered CVD-MPFA performance

Grid generation for reservoir simulation must honor classical key constraints and be boundary aligned such that control-volume boundaries are aligned with geological features such as layers, shale barriers, fractures, faults, pinch-outs, and multilateral wells. An unstructured grid generation procedure is proposed that automates control-volume and/or control point boundary alignment and yields a PEBI-mesh both with respect to primal and dual (essentially PEBI) cells. In order to honor geological features in the primal configuration, we introduce the idea of protection circles, and to generate a dual-cell feature based grid, we construct halos around key geological features. The grids generated are employed to study comparative performance of cell-centred versus cell-vertex control-volume distributed multi-point flux approximation (CVD-MPFA) finite-volume formulations using equivalent degrees of freedom. The formulation of CVD-MPFA schemes in cell-centred and cell-vertex modes is analogous and requires switching control volume from primal to dual or vice versa together with appropriate data structures and boundary conditions. The relative benefits of both types of approximation, i.e., cell-centred versus vertex-centred, are made clear in terms of flow resolution and degrees of freedom required.