A Bioassay for the Cytotoxicity of Gemcitabine Using the Marine Ciliate Euplotes vannus

This study investigated the cytotoxicity of gemcitabine using the marine ciliate Euplotes vannus as the test organism.The median lethal concentrations(LC_(50) values)were determined using acute toxicity tests within an exposure time of 30 min with 0,6,12,24,and 48 mg mL~(-1) gemcitabine.The median inhibition effect(IC_(50) value)on the growth of the ciliate cells was examined using chronic toxicity tests within 5 days(120 h)after exposure for 30 min with 0,0.7,3.5,7,and 14 mg mL~(-1) gemcitabine.The 30-min LC_(50)value was 10.66~-mg mL ~1.The LC_(50) values decreased with increasing exposure times and well fitted to the toxicity curve equation LC_(50)=10.93+28.4e~(-0.19t)(R~2=0.93;P0.05,t=exposure time).The IC_(50) value for growth rates was 7.05 mg mL~(-1),and the inhibition effect on growth rates well fitted to the model equation r_%=0.8681e~(-0.0782Cgem)(r_%means growth rate with inhibition by gemcitabine,C_(gem) means concentrations of gemcitabine,R~2=0.99 and P0.05).The LC_(50) values of a wide range of gemcitabine concentrations could therefore be predicted for any given exposure time.These results suggest that the clinical dose of gemcitabine(20 mg mL~(-1))was higher than the 30-min LC_(50) value,which was almost the same as the 6-min LC_(50) value(19.88 mg mL~(-1))for E.vannus cells.The results also demonstrate that E.vannus can be used as a robust test organism for bioassays of chemotherapeutic drugs during short exposure periods.     

Correction to: Textural Quantification and Classification of Drill Cores for Geometallurgy: Moving Toward 3D with X-ray Microcomputed Tomography (μCT)

Natural Resources Research - The original version of the article unfortunately contained an error in Table 9. In article PDF, Table 7 was inadvertently repeated in place of...     

Spatial optimization for land acquisition problems: A review of models,solution methods,and GIS support

This article reviews the interdisciplinary research field of spatial optimization for land acquisition problems. We start with a theoretical framework to identify three categories of spatial optimization models: problems with aspatial constraints, location models, and problems with topological constraints. Exact, heuristic, and metaheuristic approaches to solving these problems are critically discussed. Tools that are available in commercial and open‐source GIS packages are reviewed from four aspects. We first survey the off‐the‐shelf support and then the development environments in these packages. A case study of the one‐center problem is used to illustrate the computational performance of different solution methods. Finally the advantages and disadvantages of current GIS data models are discussed. The article concludes with challenges and future directions for solving spatial optimization problems for land acquisition.     

Retreat of the Smith Sound Ice Stream in the Early Holocene

Nares Strait, a major connection between the Arctic Ocean and Baffin Bay, was blocked by coalescent Innuitian and Greenland ice sheets during the last glaciation. This paper focuses on the events and processes leading to the opening of the strait and the environmental response to establishment of the Arctic‐Atlantic throughflow. The study is based on sedimentological, mineralogical and foraminiferal analyses of radiocarbon‐dated cores 2001LSSL‐0014PC and TC from northern Baffin Bay. Radiocarbon dates on benthic foraminifera were calibrated with ΔR = 220±20 years. Basal compact pebbly mud is interpreted as a subglacial deposit formed by glacial overriding of unconsolidated marine sediments. It is overlain by ice‐proximal (red/grey laminated, ice‐proximal glaciomarine unit barren of foraminifera and containing >2 mm clasts interpreted as ice‐rafted debris) to ice‐distal (calcareous, grey pebbly mud with foraminifera indicative of a stratified water column with chilled Atlantic Water fauna and species associated with perennial and then seasonal sea ice cover) glacial marine sediment units. The age model indicates ice retreat into Smith Sound as early as c. 11.7 and as late as c. 11.2 cal. ka BP followed by progressively more distal glaciomarine conditions as the ice margin retreated toward the Kennedy Channel. We hypothesize that a distinct IRD layer deposited between 9.3 and 9 (9.4–8.9 1σ) cal. ka BP marks the break‐up of ice in Kennedy Channel resulting in the opening of Nares Strait as an Arctic‐Atlantic throughflow. Overlying foraminiferal assemblages indicate enhanced marine productivity consistent with entry of nutrient‐rich Arctic Surface Water. A pronounced rise in agglutinated foraminifers and sand‐sized diatoms, and loss of detrital calcite characterize the uppermost bioturbated mud, which was deposited after 4.8 (3.67–5.55 1σ) cal. ka BP. The timing of the transition is poorly resolved as it coincides with the slow sedimentation rates that ensued after the ice margins retreated onto land.     

Causes of Weather-Related Crashes in Salt Lake County,Utah

In 2010, the World Health Organization declared 2011 to 2020 the Decade of Action for Road Safety in response to the enormous toll that roadway crashes take on individuals, communities, and national economies. More than 6.2 million vehicle crashes occurred in the United States in 2015, resulting in around 2.4 million injuries and more than 35,000 fatalities. Motor vehicle crashes in the United States in 2015 had an economic cost of $836 billion, equal to nearly 5 percent of the U.S. gross domestic product. For this study, more than 122,000 crashes occurring in Salt Lake County, Utah, from 2012 to 2016 were analyzed to see how weather conditions affected the number and type of crashes observed. Approximately 12 percent of crashes occurred under “adverse weather conditions.” Weather-related crashes were most likely in the winter season and were very highly correlated with monthly snowfall. Although the greatest number of crashes occurred during evening rush hours, adverse weather-related crashes made up a larger percentage of crashes from 5 to 7 a.m. Although excessive speed was much more likely to be a factor in crashes during adverse weather, such crashes, on average, were less severe. Roadway slope also played a significant role, with slight increases causing more crashes.     

Obstacle‐limited service coverage: A new geospatial approach

Service coverage is a crucial component of spatial decision‐making involving facility siting. Determining coverage requires knowledge about travel behavior and accessibility across a region, as well as supporting methods of measurement and derivation when actual travel cannot necessarily be observed. When service is provided using vehicles that move freely without the restriction posed by roads (e.g., airplanes, helicopters, unmanned aerial vehicles/drones, boats, ships, and submarines), straight‐line travel is regularly assumed. However, this common assumption is often violated by geographical obstructions, resulting in overestimated service coverage. To date, no methods have been developed capable of accounting for obstacle‐limited service coverage. Existing methods either completely ignore obstructions or crudely estimate impacts on travel. In this research, we develop a novel method, ESP‐Cover, capable of deriving accurate obstacle‐limited coverage without introducing representation errors. A location siting problem involving drone‐based package delivery is presented to highlight the necessity of explicitly accounting for obstacles in system design. The assessment results demonstrate the importance of accurate derivation of the obstacle‐limited coverage for spatial decision‐making and the accuracy of the ESP‐Cover method in coverage derivation.     

Mass mortality of the fan mussel,Pinna nobilis in Turkey (eastern Mediterranean)

This study reported the mass mortality events (MMEs) of Pinna nobilis based on diving surveys in Turkey. Data were collected across 12 sites in the Aegean Sea, and one site in the Sea of Marmara, within the period of June-September 2019. The results showed that a low mortality rate of 10% was found in the Sea of Marmara, and a MME was not detected for this area. In contrast, 97% mortality was recorded for the P. nobilis population distributed in the Turkish Aegean Sea. This discrepancy might be related to the seawater temperature and salinity values of these two seas. The waters of the Sea of Marmara were colder and less salty than the Aegean Sea. The findings highlighted the importance of protecting the healthy population of critically endangered fan mussels in the Sea of Marmara. It was recommended that sound management plans comprising of monitoring, enforcement and public awareness activities could be implemented in order to achieve this.     

Open-source approaches for location cover models: capabilities and efficiency

Journal of Geographical Systems - Location cover models are aimed at siting facilities so as to provide service to demand efficiently. These models are crucial in the management, planning and...     

TIN-based Tag Map Layout

With the widespread use of tag clouds, multiple map-based variations have been proposed. Like standard tag clouds (also called word clouds), these ‘tag maps’ all share the basic strategy of displaying words within a ‘geographic space’ and scaling the word size to depict frequency (or importance) of those words within some dataset. While some tag maps simply plot a standard tag cloud on top of a map, the subset of tag maps we focus on here are those in which the collection of words are displayed within bounded geographic regions (often of irregular shape) that the words are relevant for. For this form of tag map, map scale and polygon shape add constraints to word size and position that have not been considered in most prior approaches to tag map word layout. In this paper, we present a layout strategy for tag map generation that includes consideration of the shape and size of the geographical regions acting as containers for the tags. The method introduced here uses a triangulated irregular network (TIN) to subdivide the geographical region into many triangle subareas, with the centroid of each triangle being a potential location to centre a tag on. All the triangles are sorted by their area and all the tags are sorted by their weight value (e.g. frequency, importance or popularity). Positioning of tags is undertaken sequentially from most important (or frequent or popular) with potential locations being the TIN triangle centroids (tried from largest to smallest triangle). After each tag placement, the TIN is recalculated to integrate the tag centroid and bounding corners into the TIN creation. The limited whitespace in the geographical region, at any specific scale, is used fully by dynamically adjusting the font size along with the number and the direction of tags. The method can be applied to add tags within geographic polygons that are convex, concave and other more complex regions containing holes or islands.     

Challenges in applying capacitated covering models

Service system planning has long been supported by location‐covering models designed to address access and accessibility issues. An important aspect of many systems is limits on service, often conceptualized as facility capacities. Much research can be found that proposes modeling approaches and solution techniques to account for capacitated covering problems, and commercial GIS software exists that is capable of structuring and applying facility service limits. This article reviews issues and challenges associated with the application of capacitated covering models, including critical evaluation of allocation approaches and GIS capabilities. Case studies involving service provision in two cities in California—San Jose and Santa Barbara—are provided to highlight associated issues faced in practice. While user‐friendly commercial software makes it easy to access capacitated cover models, there remain challenges for addressing underlying considerations and assumptions in practice.