Distribution of nutrients and eutrophication assessment in the Bohai Sea of China

Water samples were collected in 120 stations in the Bohai Sea of China to analyze the distribution of dissolved nutrients and assess the degree of eutrophication in August 2002. The result shows that the average concentration of DIN increased and the PO4-P concentration sharply decreased compared to the previous data of corresponding period. The high concentrations of DIN and PO4-P occurred in coastal waters, especially in the bays and some river estuaries, while the high concentrations of SiO3-Si in the surface and middle depth occurred in the central area of the Bohai Sea. The average ratio of DIN/ PO4-P was much higher than the Redfield Ratio (16:1). Apparently, PO4-P was one of the limiting nutrient for phytoplankton growing in the sea. The average concentrations of DON and DOP were higher than their inorganic forms. The results of eutrophication assessment show that 22.1% of all stations were classified as violating the concentration levels of the National Seawater Quality Standard (GB 3097-1997) for DIN and only 3.9% for PO4-P. The average eutrophication index in the overall area was 0.21±0.22 and the high values occurred in Bohai Bay, Liaodong Bay and near the Yellow River estuary. This means that the state of eutrophication was generally mesotrophic in the Bohai Sea, but relatively worse in the bays, especially some river estuaries.     

文蛤抗癌多肽对N-乙酰-β-D氨基葡萄糖苷酶活力的影响

从文蛤体中分离纯化得到了抗癌多肽,研究该多肽对N-乙酰-β-D-氨基葡萄糖苷酶(NAGase)活力的影响,结果显示文蛤抗癌多肽对该酶有明显的抑制作用.在本文中,我们将深入地研究其抑制作用机理和抑制效应类型,结果表明:随着文蛤多肽加入量的增大,该酶活力呈指数下降,测得导致酶活力下降50%的抑制剂浓度(IC50)为112.9 μg/cm3,该多肽对酶的抑制作用是一种可逆过程,抑制机理表现为混合型抑制类型,对游离酶(E)的抑制常数(KI)和酶-底物络合物的抑制常数(KIS)分别为77.03和697.44μg/cm3,说明文蛤抗癌多肽与游离酶的结合导致酶活力的丧失,明显的强于对酶-底物络合物的抑制效应,底物的存在对该酶被文蛤抗癌多肽抑制作用起了保护作用.     

Screening of extraction methods for glycoproteins from jellyfish (Rhopilema esculentum) oral-arms by high performance liquid chromatography

In order to select an optimum extraction method for the target glycoprotein (TGP) from jellyfish (Rhopilema esculentum) oral-arms, a high performance liquid chromatography (HPLC)-assay for the determination of the TGP was developed. Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear (r = 0.9984, y = 4.5895x+47.601) over concentrations ranging from 50 to 400 mgL-1. The mean extraction recovery was 97.84% (CV2.60%). The fractions containing TGP were isolated from jellyfish (R. esculentum) oral-arms by four extraction methods: 1) water extraction (WE), 2) phosphate buffer solution (PBS) extraction (PE), 3) ultrasound-assisted water extraction (UA-WE), 4) ultrasound-assisted PBS extraction (UA-PE). The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL (7.8 mm×300 mm) column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.     

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基于Java3D海量地形数据网络发布

三维地形的网络发布，涉及数据的实时网络传输、海量地形数据的管理、地形数据的快速更新、与用户实时交互等难题。文中在采用JEE构架的基础上，探讨多比例尺的DEM数据及其相应不同分辨率DOM数据的存储、管理和快速更新，并在此基础上，利用Java3D实现基于视点LOD海量地形数据在网络上实时漫游。     

马氏珠母贝(Pinctada martensii)2个不同地理种群遗传变异的EST-SSR分析

应用17个EST-SSR位点分析表明,马氏珠母贝印度种群和三亚种群的平均FST值为0.486,两个种群的分化程度高;三亚种群的等位基因数为1—6,有效等位基因数为1.0—3.3;印度种群的等位基因数为2—7,有效等位基因数为1.1—3.3;三亚种群和印度种群的平均有效等位基因数分别是1.8和1.9;三亚种群和印度种群的等位基因丰度分别为3.16和3.39;印度种群无论是期望杂合度(HE)还是观察杂合度(HO)都高于三亚种群,印度种群的期望杂合度和观察杂合度分别是0.42和0.16;三亚种群的期望杂合度和观察杂合度分别是0.38和0.15;三亚种群与印度种群的Nei’s遗传距离为1.119。两个种群都保持了中等水平遗传多样性。由于两个种群高度的遗传分化和保持丰富的遗传多样性为开展两个种群间杂交及获得杂种优势奠定了基础。     

城市测绘电子档案质量略见

结合城市测绘的特点,规范档案工作流程,基于局域网实现电子档案信息的合理流转。建立电子档案质量监控机制,梳理常见质量问题,研发相应的质量检审工具软件,实现电子档案质量监控的智能化,避免档案再利用时因信息错误导致发生工程质量事故。     

藏、汉对比视角下的城市空间环境认知研究——以中国西藏日喀则市为例

以分布式认知理论为基础,采用质性研究法、数量分析法以及GIS空间分析技术,探讨日喀则市藏族、汉族居民对城市空间环境认知的异同性。研究表明,藏族对城市空间环境的认知得分高于汉族对城市空间环境的认知得分。藏族、汉族对城市空间环境的认知水平可分为高、中、低3类。认知空间呈现圈层结构与混合结构模式,可分为藏汉一致性、藏族独立以及汉族独立3种认知空间类型。影响居民对城市空间环境的认知因素有共性因素和差异性因素。景观走廊质量、宜居性、基础设施两项是藏族居民关注的主要影响因素,汉族居民更关注公共服务设施的服务能力以及对当地文化的融入感。     

GPS接收机的中频信号处理算法研究

该文对GPS接收机的中频信号处理算法进行了研究,内容主要涉及信号捕获、载波恢复和伪码跟踪3部分,详细分析了信号捕获过程中所采用的匹配滤波器法、快速傅里叶算法(FFT)、锁频环(FLL)、锁相环(PLL)以及延迟锁定环(DLL)的算法原理,并对环路滤波器作了相应的阐述,给出环路对应的递推公式．     

体视化关键技术

体视化技术有着广泛的应用前景。对体视化的几种关键技术进行了分类综述，介绍了体数据的表示和分类，面绘制、体绘制和混合绘制各类典型算法的原理和特点。体数据的表示方式涉及到表示精度、存储量和处理时间之间的矛盾，要根据实际问题的具体特点和要求加以选择。体数据的分类是整个可视化算法中的一个关键步骤。只有对体数据进行准确的分类，才能经过处理得出合理的图像。近年来为加快绘制速度和提高图像质量提出了许多改进的体视化算法，并对部分改进算法进行了介绍。