海洋Pseudomonasaeruginosasp.DES-3的鉴定以及基因ap02对其产蛋白酶的影响研究

海洋是地球上最大的生态系统,其多样独特的生态环境造就了其有别于其他环境的微生物资源。本研究利用蛋白酶的活性筛选策略,从辽宁省营口市某海洋淤泥中分离得到一株产蛋白酶的优势菌株DES-3。通过微生物形态学特征、生理生化性质和16SrDNA基因鉴定以及系统发育进化关系的分析,将该菌株鉴定为铜绿假单胞菌属,命名为Pseudomonas aeruginosa sp.DES-3。其胞外蛋白酶的最适反应温度和最适pH分别为55℃和9.0。将来自于碱性污染土壤宏基因组文库的编码碱性蛋白酶基因ap02与广宿主表达载体pBBR1MCS-5连接,转化至野生型菌株P.aeruginosasp.DES-3细胞中,成功构建了基因工程菌株P.aeruginosasp.DES-3/pBBR1MCS-5-ap02。基因工程菌株的最适反应温度为60℃,较野生型菌株提高了5℃。本研究构建基因工程菌株的策略可为菌株的改造和相应工业应用提供一定的技术参考。     

Observed characteristics of atmospheric ducts over the South China Sea in autumn

The observed characteristics of lower atmospheric ducts over the South China Sea(SCS) were analyzed based on Global Position Systerm(GPS) radiosonde data collected four times daily during autumn open cruises from 2006 to 2012.Duct occurrence,thickness,and strength over the SCS were about 40%,150-m thick,and 8 M units,respectively,which were larger than during the summer monsoon period.Most ducts occurred at heights 1 500 m and these ducts easily trap electromagnetic wave clusters with wavelengths 2 m.Diurnal variation of the SCS ducts appeared evident.They occurred more often at midnight at higher altitudes(about 1 100 m),with a thickest layer of about 145 m and less frequently during the evening at lower altitudes(about 800 m),with a thinnest layer of about 125 m.Moreover,ducts during the daytime at a mean height of about 900 m,with the greatest strength of about 10 M units.Furthermore,all duct variables observed over the SCS in autumn decreased from north to south.These findings are useful not only in the design of radar and communication systems,but also for evaluating possible effects of anomalous propagation on meteorological radar and military applications.     

The morphological characteristics of gully systems and watersheds in Dry-Hot Valley,SW China

Gully systems and watersheds are geomorphic units with clear boundaries that are relatively independent of basin landscapes and play an important role in natural geography. In order to explore the morphological characteristics of gully systems and watersheds in the Dry-Hot Valley [South West (SW) China], gullies are interpreted from online Google images with high resolution and watersheds are extracted from digital elevation model at a scale of 1:50,000. The results show that: (1) There are 17,382 gullies (with a total area of 1141.66 km²) and 42 watersheds in the study area. (2) The average gully density of the study area (D) is 4.29 km/km², gully frequency (F) is 14.39 gullies/km², the branching ratio (B) is 5.13, the length ratio (L) is 3.12, and the coefficient of the main and tributary gullies (M) is 0.06. The degree of gully erosion is strong to extremely strong, the main development intensity of gully erosion ranges from intense to moderate, and the type of gully system is tributary. (3) The watershed areas (A) are between 0.39 and 96.43 km², the relief ratio (R) is from 0.10 to 0.19, the circularity ratio (C) is from 0.30 to 0.83, the texture ratio (T) is from 0.82 to 39.35, and the dominant geomorphological texture type is fine. (4) There is a quantitative relationship between F and D:F?=?0.624D² (R?=0.84) and T is closely related to D, F, M (R²?>?0.7). A, R and C are related to M (R²?>?0.5). The development of gully systems is the result of coupling effects between multiple factors. In this area, the degree of erosion and the condition of the main and tributary gullies can be controlled by the degree of topographic breakage in the watershed, which provides some theoretical basis for the evaluation of gully erosion by the latter. In addition, the scale, relief, and shape have a significant impact on the locations of the main and tributary gullies. For tributary gullies, attention should be paid to the interception and control of runoff and sediment in the small confluence branches in order to prevent gully expansion and head advance. These features can inform the development of targeted measures for the control of soil erosion.     

基于Pasternak双参数地基模型的陡坡段桥梁基桩内力计算方法

为考虑土体剪切模量对陡坡段桥梁基桩内力计算的影响,首先探讨桩后土压力的分布形式,并根据陡坡段桥梁基桩的承载特性建立简化分析模型;其次,引入Pasternak双参数模型,建立可考虑土体剪切模量的地基抗力计算模型;并在此基础上分别对受荷段与嵌固段基桩微元进行受力分析,求得各段控制差分方程,从而进一步迭代求解桩身位移及内力;最后,分别用陡坡段桥梁基桩实测数据与Pasternak双参数模型算例对本文内力计算方法进行验算,结果表明:本文计算方法用于陡坡段桥梁基桩内力计算是合理的,可为同类工程提供参考。     

山东黄金西岭勘查区深部勘探孔防斜与纠斜措施

西岭勘查区金矿找矿工作已全面进入勘探阶段,勘查区网度为80 m×80 m,对于钻孔质量要求十分严格：终孔靶点偏差不超过勘探网度的四分之一,即不超过20 m。对于西岭勘查区设计孔深均超过1500 m（部分钻孔设计甚至超过2500 m）的钻孔而言,这种要求远远超过地质岩心钻探规程要求。山东黄金地勘公司根据多年深孔施工经验,在防斜与纠斜方面均取得了一些突破,自主研发了钻具扶正器、独创了钻孔纠斜法,形成了一套实用的钻孔质量保证措施。     

The Fragmentation in a Protoplanetary Disk and the Properties of the Resultant Self-Gravitating Clumps

We study the fragmentation properties in the protoplanetary disk and properties of the resultant self-gravitating clumps using our newly constructed disk model. Our disk model includes the mass inflall term from a molecular cloud core and the photoevaporation winds effect. We adopt the conventional fragmentation criterion to judge whether a protoplanetary disk can fragment. In this work, we follow our previous work to investigate the properties of the resultant self-gravitating clumps. In our calculation, the initial masses of the resultant self-gravitating clumps lie in the range of tens of M_J to more than one hundred of M_J, where M_J is the Jupiter mass. These initial masses can seemingly account for the masses of extrasolar planets in magnitude. We also calculate the subsequent gas accretion of clumps in 1.27 × 10⁴ yr after the formation of self-gravitating clumps. We find that the subsequent gas accretion of self-gravitating clumps is very efficient, and the clump masses grow to hundreds of M_J and the physical radii R_c of clumps increase to about 10 AU. Additionally, we also calculate the orbital migration of clumps. We find that most clumps have short migration timescale to be accreted onto the protostar, and only a small fraction of clumps have long migration timescale (>10⁶ yr) to successfully become gas giant planets. These results are consistent with previous studies.