Intercolony variability of skeletal oxygen and carbon isotope signatures of cultured Porites corals: Temperature-controlled experiments

The skeletal oxygen isotope ratio of Porites corals is the most frequently used proxy of past seawater temperature and composition for tropical and subtropical oceans. However, field calibration of the proxy signals is often difficult owing to the dual dependence of skeletal oxygen isotope ratio on temperature and the oxygen isotope composition of water. We conducted tank experiments in which we grew Porites spp. colonies for 142 d in thermostated seawater at five temperature settings between 21°C and 29°C under moderate light intensity of 250 μmol m⁻² s⁻¹ with a 12:12 light:dark photoperiod. A skeletal isotope microprofiling technique applied along the major growth axis of each colony revealed that the oxygen isotope ratios of newly deposited skeleton in most colonies remained almost constant during tank incubation, thus providing an ideal situation for precise calibration of oxygen isotope ratio proxy signals. However, the oxygen isotope ratios displayed an unusually large intercolony variability (∼1‰) at each temperature setting although the mean slope (∼0.15‰ °C⁻¹) obtained for the temperature-skeletal oxygen isotope ratio relationship was close to previous results. The intercolony variations in the oxygen isotope ratios were apparently caused by kinetic isotope effects related to variations in the skeletal growth rate rather than by species-specific variability or genetic differences within species. No correlation was found between skeletal carbon isotope ratios and temperature. The carbon isotope ratios showed significantly inverse correlation with linear growth rates, suggesting a kinetic isotope control at low growth rates. Observed intercolony variability in skeletal carbon isotope ratios (∼5‰) can be partly attributed to growth-rate-related kinetic isotope effects.     

Comparison of runoff characteristics of two adjacent basins in a tropical rainforest using a modified hydrologic cycle model with outflow

We propose a new runoff model including an outflow process that was applied to two adjacent basins (CL, TL) located in Lambir Hills National Park in north‐central Sarawak, Malaysia. Rainfall, runoff, topography, and soil layer thickness were observed. About 19% of annual runoff was observed in the CL basin (21.97 ha), whereas about 46% was observed in the TL basin (23.25 ha). It was inferred that the CL basin has an outflow because of low base flow, small runoff peak, and excessive water loss. By incorporating the outflow process into the HYdrological CYcle MODEL, good agreement between the data generated by the model and that observed was shown, with the exception of the data from the rainless period. Then, the fitting parameters for each basin were exchanged, except for the outflow parameter, and the characteristics of each basin were compared by calculating virtual runoff. As a result, the low base flow of the CL basin was estimated by the movement of the rainwater that escaped from the basin as deep percolation or lateral flow (11% of rainfall). The potential of the CL basin for mitigating flood and drought appeared to be higher than that of the TL basin. This is consistent with the topographic characteristics of the CL basin, which has a gentler slope than the TL basin. Copyright © 2012 John Wiley & Sons, Ltd.     

Degradation and utilization of protein derived from <Emphasis Type="Italic">Pseudomonas aeruginosa</Emphasis> by marine microbial community

Microbial degradation and utilization of proteins derived from bacterial detritus were investigated in a microcosm experiment using Pseudomonas aeruginosa detritus as a substrate. To assess the effects of natural marine microbial communities on degradation and utilization of protein derived from P. aeruginosa cells, four microcosms were prepared: natural seawater (containing the natural microbial community) with P. aeruginosa detritus (N+Pa), autoclaved seawater with P. aeruginosa detritus (A+Pa), natural seawater (N) and autoclaved seawater (A) without adding anything as a control. The numbers of total and growing bacterial cells, protease activity, and transition of P. aeruginosa proteins were monitored in the four microcosms. Changes in the numbers of total and growing bacterial cells and protease activities indicated that bacterial detritus significantly stimulated the microbial community in the microcosms. Both the surviving P. aeruginosa in A+Pa and natural microbial community in N+Pa microcosms were able to degrade and utilize P. aeruginosa detritus; however, the community in N+Pa including various microbes maintained high activity longer, indicating that diversity is an important factor in keeping the community active. Even under the very high protease activity in N+Pa, 39-kDa and 48-kDa proteins from P. aeruginosa remained in the microcosm during the entire experiment (150 days). Immunoblotting suggested the 48-kDa protein was an intact molecule of OprP, which had been detected from the dissolved fraction of natural seawater in previous studies. This result suggests that the protein molecules that had been detected from natural seawater actually had a high tolerance to microbial degradation.     

沙虫干呈味及功能性成分研究

对沙虫干抽提物成分的组成进行的研究结果表明 :游离氨基酸含量 w为 10 .3% ,占抽提物含氮物质的 77.9%。抽提物组成中 ,呈甘味的 Gly(3.2 % )、Ala(2 .5% )和呈鲜味的 IMP(0 .2 7% )、Glu(0 .2 5% )、琥珀酸 (0 .35% )等成分同沙虫干独特的风味有关 ,Na 、Cl-等无机离子也同其呈味相关。此外 ,牛磺酸含量 w高达 3.2 % ,是富含牛磺酸的一种海洋生物 ;精氨酸的含量 w亦较高 ,为 1.2 %。     

Origin of calcium in aerosols over the western North Pacific

Weekly aerosol samples were collected from March 1981 to June 1983 at the six stations in the western North Pacific region and analyzed for Ca and Na. By coupling data with those previously reported for Al (Tsunogai et al., 1985), the following results and conclusion have been obtained. There was a positive correlation between the atmospheric concentration of Al and the concentration of nonsea salt Ca (nssCa). The nssCa/Al ratios from the six stations, however, considerably varied (from 0.84±0.36 to 3.00±1.91), and the ratios were usally larger than those of the crustal average or of usual soil in Japan. The Ca/Al ratios of Asian desert soil and loess vary from 0.52 to 1.29, which are similar to the nssCa/Al ratios of aerosols in the surface air over the western North Pacific region except at Onna, Okinawa. The exception may be due to a local effect of coral. These results suggest that a large part of nonsea salt Ca in the surface air over the western North Pacific is derived from arid regions in Asia and that the nssCa/Al ratio in aerosol varies with that of the source material.     

Characteristics of amorphous kerogens fractionated from terrigenous sedimentary rocks

A preliminary attempt to fractionate amorphous kerogens from terrigenous bulk kerogen by a benzene-water two phase partition method under acidic condition was made. Microscopic observation revealed that amorphous kerogens and structured kerogens were fractionated effectively by this method. Characteristics of the amorphous and structured kerogens fractionated by this method were examined by some chemical analyses and compared with those of the bulk kerogen and humic acid isolated from the same rock sample (Haizume Formation, Pleistocene, Japan). The elemental and infrared (IR) analyses showed that the amorphous kerogen fraction had the highest atomic$\text{H}\text{C}$ ratio and the lowest atomic $\text{N}\text{C}$ ratio and was the richest in aliphatic structures and carbonyl and carboxyl functional groups. Quantities of fatty acids from the saponification products of each geopolymer were in agreement with the results of elemental and IR analyses. Distribution of the fatty acids was suggestive that more animal lipids participate in the formation of amorphous kerogens because of the abundance of relatively lower molecular weight fatty acids (such as C₁₆ and C₁₈ acids) in saponification products of amorphous kerogens. On the other hand, although the amorphous kerogen fraction tends to be rich in aliphatic structures compared with bulk kerogen of the same rock samples, van Krevelen plots of elemental compositions of kerogens from the core samples (Nishiyama Oil Field, Tertiary, Japan) reveal that the amorphous kerogen fraction is not necessarily characterized by markedly high atomic $\text{H}\text{C}$ ratio. This was attributed to the oxic environment of deposition and the abundance of biodegraded terrestrial amorphous organic matter in the amorphous kerogen fraction used in this work.     

Double seismic zone beneath the middle of Hokkaido, Japan, in the southwestern side of the Kurile Arc

The vertical section of microearthquakes, determined accurately by using the Hokkaido University network, shows two dipping zones (the double seismic zone) 25–30 km apart in the depth range of 80–150 km beneath the middle of Hokkaido in the southwestern side of the Kurile arc. Hypocentral distribution of large earthquakes (m_b > 4) based on the ISC (International Seismological Centre) bulletin also shows the double seismic zone beneath the same region. The hypocentral distribution indicates that the frequency of events occurring in the lower zone is four times greater than that in the upper zone. The difference in seismic activity between the two zones beneath Hokkaido is in contrast with the region beneath northeastern Honshu in the northeastern Japan arc.Composite focal mechanisms of microearthquakes and individual mechanisms of large events mainly characterize the down-dip extension for the lower zone as is observed beneath northeastern Honshu. For the upper zone, however, the stress field is rather complex and not necessarily similar to that beneath northeastern Honshu. This may be considered to indicate the influence of slab contortion or transformation in the Hokkaido corner between the Kurile and the northeastern Japan arcs.