Effects of organochlorine compounds on cytochrome P450 aromatase activity in an immortal sea turtle cell line

Many classes of environmental contaminants affect the reproductive function of animals through interactions with the endocrine system. The primary components affected by endocrine active compounds (EACs) are the steroid receptors and the enzymes responsible for steroidogenesis. This study sought to develop an in vitro model for assessing EAC effects in sea turtles by examining their ability to alter cytochrome P450 aromatase (CYP19) activity. Aromatase is the enzyme responsible for the conversion of testosterone to estradiol. This enzyme is critical in the sexual differentiation of reptiles which demonstrate temperature-dependent sex determination. An immortal testis cell line GST-TS from a green sea turtle was grown in culture at 30 degrees C in RPMI 1640 media. The cells were exposed to three known aromatase inducers; dexamethasone (Dex), 8Br-cyclic AMP, or human chronic gonadotropin (HCG) and one aromatase inhibitor 4-androstenol-dione (4-OHA). In addition, the GST-TS cells were exposed to 0.1-30 microM atrazine and 3-100 microM 4,4'-DDE. The inducing compounds that have been shown to increase aromatase activity in other systems failed to induce aromatase activity in the GST-TS cells, yet exposure to the inhibiting compound, 4-OHA, did result in a significant reduction. Atrazine (0.1, 1.0 and 10 microM) significantly induced aromatase activity following a 24 h exposure, and 4,4'-DDE inhibited the activity but only at cytotoxic concentrations (100 microM). Based on these results, this in vitro model can be useful in examining the endocrine effects of EACs in sea turtles.     

Spectral investigation of the chromosphere

Several hundred H spectrograms from areas close to the solar limb were taken with the 35 cm Coudé refractor at Anacapri. The 41 spectra with the greatest spatial resolution were selected and analysed. At the supergranular boundaries a considerable fraction of the line profiles were found to correspond to Beckers Cloud Model (BCM). Moreover, the BCM parameters of the dark mottles at the limb appear to be approximately equal to those from the center of the disk. On the other hand, we also obtained evidence in disagreement with the general applicability of the BCM to all features of the chromospheric fine structure.We attempted to present the large set of observational data in a fashion that permits their interpretation by alternative theoretical models.Mitteilungen aus dem Fraunhofer Institut Nr. 151.     

Incorporating Spatial Dependence in Predictive Vegetation Models: Residual Interpolation Methods*

Predictive vegetation modeling can be used statistically to relate the distribution of vegetation across a landscape as a function of important environmental variables. Often these models are developed without considering the spatial pattern that is inherent in biogeographical data, resulting from either biotic processes or missing or misspecified environmental variables. Including spatial dependence explicitly in a predictive model can be an efficient way to improve model accuracy with the available data. In this study, model residuals were interpolated and added to model predictions, and the resulting prediction accuracies were assessed. Adding kriged residuals improved model accuracy more often than adding simulated residuals, although some alliances showed no improvement or worse accuracy when residuals were added. In general, the prediction accuracies that were not increased by adding kriged residuals were either rare in the sample or had high nonspatial model accuracy. Regression interpolation methods can be an important addition to current tools used in predictive vegetation models as they allow observations that are predicted well by environmental variables to be left alone, while adjusting over‐ and underpredicted observations based on local factors.     

Importance of climate,forest fires and human population size in the Holocene boreal forest composition change in northern Europe

The relative importance of climate, forest fires and human population size on long‐term boreal forest composition were statistically investigated at regional and local scales in Fennoscandia. We employ pollen data from lakes, reflecting regional vegetation, and small forest hollows, reflecting local vegetation, from Russia, Finland and Sweden to reconstruct the long‐term forest composition. As potential drivers of the Holocene forest dynamics we consider climate, generated from a climate model and oxygen isotope data, past forest fires generated from sedimentary charcoal data and human population size derived from radiocarbon dated archaeological findings. We apply the statistical method of variation partitioning to assess the relative importance of these environmental variables on long‐term boreal forest composition. The results show that climate is the main driver of the changes in Holocene boreal forest composition at the regional scale. However, at the local scale the role of climate is relatively small. In general, the importance of forest fires is low both at regional and local scales. The fact that both climate and forest fires explain relatively small proportions of variation in long‐term boreal vegetation in small forest hollow records demonstrates the complexity of factors affecting stand‐scale forest dynamics. The relative importance of human population size was low in both the prehistorical and the historical time periods. However, this is the first time that this type of data has been used to statistically assess the importance of human population size on boreal vegetation and the spatial representativeness of the data may cause bias to the analysis.     

The relation of Holocene fluvial terraces to changes in climate and sediment supply,South Fork Payette River,Idaho

Well-preserved Holocene terraces along the South Fork Payette River in central Idaho provide a record of fluvial system behavior in a steep mountain watershed characterized by weathered and erodible Idaho Batholith granitic rocks. Terrace deposit ages were provided by ¹⁴C dating of charcoal fragments and optically stimulated luminescence (OSL) dating of sandy sediments. Along with pairing of many terrace tread heights, these data indicate episodic downcutting during the Holocene, with a mean incision rate of ～0.9 m/ka from ～7 ka to present. Prior to 7 ka, the river incised to within～3 m of current bankfull, but then aggraded by ～5 m over at least a ～10 km-long reach in an episode centered ～7–6 ka. Aggradation may relate to (1) increased hillslope sediment input from landslides and debris flows in steep tributary basins with abundant grussified granitic bedrock, (2) possible local landslide-damming of the channel, (3) decreased peak discharge, or (4) a combination of these factors. Middle Holocene channel aggradation ca. 7–6 ka corresponds with a period of prolonged and widespread aridity in the northern Rocky Mountains. Between ～5 and 1.3 ka, the river aggraded slightly and then remained stable, forming a prominent terrace tread at ～3 m above current bankfull. Modest aggradation to vertical stability of the South Fork Payette River at the 1.5 m terrace level ～1.0–0.7 ka corresponds with large fire-related debris flows in tributaries during Medieval droughts. Three intervals of incision (～5.5–5 ka, 1.3–1.0 ka and 0.5 ka) correspond with frequent but small fire-related sedimentation events and generally cooler, wetter conditions suggesting increased snowmelt runoff discharges. Other possible drivers of channel incision include an increase in stochastic or climate-modulated large storms and floods and a reduction in delivery of hillslope sediment to the channel. Aggradation is more confidently tied to climate through increases in hillslope sediment delivery and (or) decreased stream power, both likely related to warmer, drier conditions (including high-severity fires) that reduce snowmelt and decrease vegetation cover on steep slopes. Thus, the Holocene terraces of the South Fork Payette River do not reflect simple stepwise incision with periods of vertical stability and lateral migration, but record substantial episodes of aggradation as well. We infer that increases in hillslope erosion and mass movements combined with reduced discharges during prolonged droughts episodically reverse the post-glacial trend of downcutting, in particular during the middle Holocene. The present bedrock-dominated channel implies a strong tendency toward incision in the late Holocene.     

Modeling regional landslide susceptibility using dynamic soil moisture profiles

A landslide susceptibility mapping study was performed using dynamic hillslope hydrology. The modified infinite slope stability model that directly includes vadose zone soil moisture (SM) was applied at Cleveland Corral, California, US and Krishnabhir, Dhading, Nepal. The variable infiltration capacity (VIC-3L) model simulated vadose zone soil moisture and the wetness index hydrologic model simulated groundwater (GW). The GW model predictions had a 75% NASH-Sutcliffe efficiency when compared to California’s in-situ GW measurements. The model performed best during the wet season. Using predicted GW and VIC-3L vadose zone SM, the developed landslide susceptibility maps showed very good agreement with mapped landslides at each study region. Previous quasi-dynamic model predictions of Nepal’s hazardous areas during extreme rainfall events were enhanced to improve the spatial characterization and provide the timing of hazardous conditions.