HPLC法同时测定滋阴温阳活血颗粒中两种指标性成分的研究

目的：建立滋阴温阳活血颗粒中两种指标性成分（腺苷和山奈酚-3-O-β-D-葡萄糖苷）的同时测定方法。方法：采用HPLC法，选用phenomex Kinetex C_{18色谱柱（4.6mm×250mm，5μm），0.1%磷酸-乙腈为流动相，梯度洗脱，流速1.0ml/min，柱温30℃，进样量10μm，检测波长254nm；以腺苷和山奈酚-3-O-β-D-葡萄糖苷为指标性成分，共测定10批滋阴温阳活血颗粒。结果：10批颗粒中，腺苷的含量在2.706～4.132mg/g范围内，山奈酚-3-O-β-D-葡萄糖苷的含量在3.692～4.948mg/g范围内。结论：本研究所建立方法快速、准确、高效，能够对滋阴温阳活血颗粒的质量进行科学评价。}     

日照市露天开采矿山地质环境问题及恢复治理措施

通过1∶5万矿山地质环境调查和遥感解译手段,对日照市矿山进行了全面的调查,查明各类矿山546处,主要集中在莒县和五莲县境内。除莒县刘官庄煤矿外,其余全部为露天开采矿山,主要选用直接开挖的方式,不仅破坏了地表环境,而且矿山生产活动产生的固体废弃物占用了大面积的土地。该文根据矿山地理位置及开采方式的不同,将相同类型的多个矿山划分为同一治理区,针对不同治理区的特点,采取科学有效的治理方法,建立健全矿山地质环境监督管理机制,切实解决矿山地质环境问题,改善生态环境。     

不同类型的高位虾池排污对秋茄幼苗生长的影响

中国多数红树林陆地边缘存在着大量的养虾池塘。这些养虾池塘可通过：（1）换塘外排废水，（2）清塘直排污泥影响周围的红树林。本研究共包含两个实验，来探讨这两种类型的排污对秋茄幼苗生长的影响。实验一模拟虾池常规换塘情况，研究换塘废水对秋茄幼苗生长的影响。实验二设置四种虾池清塘污泥沉积厚度（0 cm，2 cm，4 cm，8 cm），研究虾池清塘污泥的累积效应对秋茄幼苗生长的影响。结果如下：在5，15和25三种盐度下，虾池换塘废水未对秋茄生长指标造成显著影响；而清塘污泥对秋茄生长的影响与其沉积厚度有关，大多数生长参数在污泥沉积厚度为4 cm时呈现最大值。秋茄幼苗在适量的沉积厚度下加速了生长，且本实验秋茄幼苗对虾池清塘污泥的耐受性为沉积厚度8 cm。     

贵州夜郎湖表层水体地球化学昼夜变化及其影响因素

为探究岩溶水库水文地球化学行为过程,对贵州普定夜郎湖表层水体进行了为期3 d的高分辨率昼夜监测。结果表明：1）多变的天气和水文条件叠加导致水体离子指标昼夜变化不显著,规律性较差。而水温、DO、pH值、SpC、SIc、pCO₂等常规理化指标受水温变化和生物作用表现出明显的昼夜波动。2）利用亨利常数和主成分分析,得到温度变化、生物作用、人类活动、水库的蓄水与放水对夜郎湖水库水文地球化学特征变化的贡献率分别为21.66%、17.28%、14.08%和10.22%,说明作用于水库水文地球化学行为的因子具有多元性。3）δ ¹⁸O表现出与DO一致的波动趋势,即白天上升,晚上下降,反映在短时间尺度上,氢氧稳定同位素变化受控于生物过程（主要是呼吸作用过程）;而对比水库水体和大气降水的d-excess（d值）发现,水库水体的d值（8.21‰）显著偏低于当地大气降水的d值（9.64‰）,说明在长时间尺度上,主要受蒸发效应引起的不平衡分馏影响。     

Influence of Stress on Void Ratios of Compacted Crushed Rock Masses in Coal Mine Gobs

The transfer and evolution of stress among rock blocks directly change the void ratios of crushed rock masses and affect the flow of methane in coal mine gobs. In this study, a Lagrange framework and a discrete element method, along with the soft-sphere model and EDEM numerical software, were used. The compaction processes of rock blocks with diameters of 0.6, 0.8, and 1.0 m were simulated with the degrees of compression set at 0%, 5%, 10%, 15%, 20%, and 25%. This study examines the influence of stress on void ratios of compacted crushed rock masses in coal mine gobs. The results showed that stress was mainly transmitted downward through strong force chains. As the degree of compression increased, the strong force chains extended downward, which resulted in the stress at the upper rock mass to become significantly higher than that at the lower rock mass. It was determined that under different degrees of compression, the rock mass of coal mine gobs could be divided, from the bottom to the top, into a lower insufficient compression zone (ICZ) and an upper sufficient compression zone (SCZ). From bottom to top, the void ratios in the ICZ sharply decreased and those in the SCZ slowly decreased. Void ratios in the ICZ were 1.2–1.7 times higher than those in the SCZ.

     

南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测

研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。     

A case study on Landscape Component Niche based on Landscape Pattern Indices: Yanchi,Ningxia Province,China

Yanchi County is located in the agro-pastoral ecotone and belongs to the ecologically fragile area of Northwest China.It is important to study the evolution of landscape pattern to curb its environmental degradation.In order to intuitively show how the landscape pattern of the study area changes over time,Landsat Thematic Mappers(TM)and Landsat Operational Land Imager(OLI)data of 1991,2000,2010 and 2017 were used.This paper attempts to apply niche theories and methods into landscape ecology,and constructs a niche model of landscape components by using"n-dimentional hypervolume niche theory"and landscape pattern indices.By evaluating the spatial and temporal evolution of niche from the perspective of two-dimensional space to reflect the changes of landscape pattern in the study area over the past 26 years,new theories and methods were introduced for the characterization of landscape pattern.The results indicate that:1)The larger the attribute and dominance value of landscape components,the higher the ecological niche and the stronger the control effect on the overall landscape.2)The ecological niche of each landscape component was significantly different,just as its control effect on the overall landscape.3)The dynamic change of the ecological niche of each landscape component was different,with grassland,unused land and arable land always in a high dominant position,although the ecological niche of construction land and water area was always low.In general,the introduction of niche theory into the landscape ecology provided a new method to study the changes in regional landscape pattern.     

生物土壤结皮对风沙土和黄绵土膨胀特性的影响

作为重要的土壤物理性质，膨胀性在影响土壤导水性、持水性、抗蚀性以及土壤结构的形成和发育等方面发挥着重要作用。为了探讨生物土壤结皮（BSCs）土壤的膨胀特性及其主要影响因素，针对黄土高原风沙土和黄绵土两种典型土壤，利用膨胀仪测定并比较了有、无藓结皮及其在不同因素（初始含水量、干湿循环、冻融循环、温度）下膨胀率的差异，分析了BSCs对土壤膨胀性的影响及其与环境因素和BSCs性质的关系。结果显示：风沙土上藓结皮的膨胀率为1.93%，较无结皮增加了8.65倍；而黄绵土上藓结皮的膨胀率为2.05%，与无结皮相比降低了76.68%。藓结皮的生物量和厚度与其膨胀率在风沙土上均呈线性正相关关系（P < 0.05），在黄绵土上分别呈二次函数（P=0.02）和线性正相关关系（P=0.02）。初始含水量同时影响了土壤最大膨胀率和稳定膨胀时间，影响程度风沙土远大于黄绵土（包括藓结皮和无结皮）；干湿循环次数对无结皮土壤膨胀率的影响程度大于藓结皮土壤，其中风沙土和黄绵土上无结皮的膨胀率分别是50.00%~620.00%和-2.28%~10.81%，而两种土壤上藓结皮的膨胀率分别是-5.70%~10.88%和-10.24%~-21.46%；冻融循环下4种土壤的膨胀率均有不同程度的降低，降幅为0~18.54%。黄绵土无结皮的膨胀率受温度影响程度较大，50℃下黄绵土无结皮的膨胀率分别是25℃和35℃下的1.17倍和1.21倍。BSCs显著地改变了风沙土和黄绵土表层的膨胀性，其影响的程度和方向取决于土壤类型。同时，BSCs的膨胀性受含水量、温度、干湿以及冻融循环等关键因素影响。     

Analysis of Spatial–Temporal Evolution of Mining-Induced Fracture Field: A Case Study Using Image Processing in the Shaqu Coal Mine,China

Natural Resources Research - Mining-induced fracture plays a key role in gas drainage for gas burst-prone underground coal mines, especially for closely multilayered coal seams. The layout and...     

滨海城市竖向避难场所选址可靠性评价与若干问题探讨

关注滨海城市竖向避难场所的选址可靠性问题，首先深化确立“竖向避难场所”概念；然后构建竖向避难场所选址可靠性评价模型，综合自然地理要素和建成环境要素，从与灾害直接作用相关的选址暴露性、与应急交通相关的选址敏感性、与应急服务相关的选址适应性3个维度展开，搭建3级评价指标体系；再以天津滨海新区为例、聚焦滨海城市潮洪灾害和人口安全矛盾的集核——临港城区展开实证研究，借助ArcGIS分类与可视化发现：高低可靠性选址之间具有明显的空间分异，可以分解为灾害暴露性的“近岸高?远岸低”分异、交通敏感性的“外围高?中心低”分异、服务适应性的“中心与沿河高?外围低”兼“近港高?远港低”分异。同时发现：商业设施用地选址价值较高，在高可靠性选址中占比第一；中小学和社会福利设施用地选址价值最高，在高可靠性选址中占比第二；文化科研和娱乐康体设施用地在高可靠性选址中占比最小。针对临港城区实证研究结果，提出滨海城市竖向避难场所选址及可靠性提升对策。