日照市露天开采矿山地质环境问题及恢复治理措施

通过1∶5万矿山地质环境调查和遥感解译手段,对日照市矿山进行了全面的调查,查明各类矿山546处,主要集中在莒县和五莲县境内。除莒县刘官庄煤矿外,其余全部为露天开采矿山,主要选用直接开挖的方式,不仅破坏了地表环境,而且矿山生产活动产生的固体废弃物占用了大面积的土地。该文根据矿山地理位置及开采方式的不同,将相同类型的多个矿山划分为同一治理区,针对不同治理区的特点,采取科学有效的治理方法,建立健全矿山地质环境监督管理机制,切实解决矿山地质环境问题,改善生态环境。     

不同类型的高位虾池排污对秋茄幼苗生长的影响

中国多数红树林陆地边缘存在着大量的养虾池塘。这些养虾池塘可通过：（1）换塘外排废水，（2）清塘直排污泥影响周围的红树林。本研究共包含两个实验，来探讨这两种类型的排污对秋茄幼苗生长的影响。实验一模拟虾池常规换塘情况，研究换塘废水对秋茄幼苗生长的影响。实验二设置四种虾池清塘污泥沉积厚度（0 cm，2 cm，4 cm，8 cm），研究虾池清塘污泥的累积效应对秋茄幼苗生长的影响。结果如下：在5，15和25三种盐度下，虾池换塘废水未对秋茄生长指标造成显著影响；而清塘污泥对秋茄生长的影响与其沉积厚度有关，大多数生长参数在污泥沉积厚度为4 cm时呈现最大值。秋茄幼苗在适量的沉积厚度下加速了生长，且本实验秋茄幼苗对虾池清塘污泥的耐受性为沉积厚度8 cm。     

地理试题透视六大解题思维——以浙江省选考试题为例

思维是指人脑借助于语言对客观事物的概括和间接反应的过程,以感知为基础,又超越感知的界限,它探索与发现事物的内部本质联系和规律性,是认识过程的高级阶段。目前国内针对地理解题的思维研究相对较少,本文以浙江省历年选考地理试题为例,总结和提炼出六大解题思维,即:逆向思维、收支平衡思维、比较思维、发散思维、空间思维、辩证思维,旨在帮助学生掌握分析和解答地理问题的方法,从而有效提升高中地理学习和复习效果,增强学生学习地理的兴趣。同时在培养学生的地理解题思维过程中,落实地理学科核心素养的培育。     

南极罗斯海沉积物中可培养菌株的分离及胞外水解酶活性检测

研究了南极罗斯海地区沉积物中可培养微生物多样性,并检测了菌株胞外酶活性,为开发利用南极资源奠定基础。采用Zobell2216E培养基、土豆培养基(PDA)及稀释平板划线法分离并获得纯培养的细菌和真菌,分别通过16SrDNA、18S/ITS序列鉴定分析了可培养微生物的多样性和系统发育。从南极罗斯海沉积物样品中获得78株细菌和54株真菌,细菌、真菌的优势菌株分别为嗜冷杆菌属(Psychrobacter)、枝孢属(Cladosporium);78株细菌菌株中有2株与模式菌株的16SrDNA基因序列相似性小于97%,可能是潜在的细菌新物种。细菌API20NE生理生化和真菌产胞外酶活性结果表明,获得的细菌和真菌大多数可检测到胞外水解酶活性。本研究丰富了南极可培养微生物资源,为南极罗斯海地区可培养微生物的多样性研究以及南极微生物资源的应用研究奠定了基础。     

Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

三元插层材料的合成及其在聚丙烯老化中的应用

以FeCl3?6H2O、NiCl2?6H2O、MgCl2?6H2O为原料，以NaOH为沉淀剂采用水热法合成了镁镍铁水滑石 (MgNiFe-LDH)，通过X射线衍射 (XRD)、傅里叶变换红外光谱 (FTIR) 和扫描电镜 (SEM) 分析了MgNiFe-LDH结构及形貌，探讨了不同陈化时间和水热温度对MgNiFe-LDH合成的影响。将制得的MgNiFe-LDH添加至聚丙烯 (PP) 中，制备MgNiFe-LDH/PP复合材料，通过对材料的加速老化测其力学性能，研究了添加MgNiFe-LDH前后材料力学性能的差异。研究结果表明，当Mg2+:Ni2+:Fe3+=2:1:1，反应温度为140℃、陈化时间为24 h时，合成的MgNiFe-LDH为片状，形貌较为规整，颗粒粒径约为2μm；经加速老化后，填充MgNiFe-LDH 复合材料的抗老化性能优于纯PP，当MgNiFe-LDH的填充量为6 wt% 复合材料拉伸强度不变，可明显提高PP的抗老化性能。     

Determination of the regional deformation rates of Shanghai and Kashima VLBI stations based on ITRF97

The vertical deformation rates (VDRs) and horizontal deformation rates (HDRs) of Shanghai VLBI station in China and Kashima and Kashima34 VLBI stations in Japan were re-analysed using the baseline length change rates from Shanghai to 13 global VLBI stations, and from Kashima to 27 stations and from Kashima34 to 12 stations, based on the NASA VLBI global solution glb1123 (Ma, 1999). The velocity vectors of the global VLBI stations were referred to the ITRF97 reference frame, and the Eulerian vectors of different models of plate motion were used for comparative solutions. The VDR of Shanghai station is estimated to be −1.91±0.56 mm/yr, and those of Kashima and Kashima34 stations, −3.72±0.74mm/yr and −8.81±0.84mm/yr, respectively. The difference between the last two was verified by further analysis. Similar estimates were also made for the Kokee, Kauai and MK_VLBA VLBI stations in mid-Pacific.     

LHRH-A和hCG对南方鲶血清促性腺激素水平的影响

运用放射免疫分析法测定了促黄体释放激素类似物 (LHRH A)和人绒毛膜促性腺激素(hCG)对南方鲶血清促性腺激素 (GTH)水平的影响 ,结果表明 :LHRH A和hCG都能提高南方鲶一龄幼鱼血清GTH水平 ,hCG与LHRH A结合注射后血清GTH水平显著高于单独注射hCG或LHRH A后的血清GTH水平。LHRH A和hCG结合注射可诱导性成熟南方鲶排卵 ,同时排卵过程中伴随着血清GTH水平的急剧上升 ,在排卵后迅速下降 ;注射同样剂量的药物而未排卵的血清GTH水平虽有升高 ,但升高的幅度不及排卵鱼。这种血清GTH水平的急剧增高对诱导南方鲶卵母细胞最后成熟和排卵具有重要作用。     

A STUDY ON LAND SYSTEM INNOVATIONS IN THE CONSTRUCTION OF SMALL TOWNS IN CHINA

The economic development in China and the rising of the living standard need to speed up the urbanisa-tion.The development of small towns is an important way to Chinese urbanization.Land use plays a very important role in the development of small towns.However there are many problems in the development of small towns,esp.in land use.The paper first discusses the land problems in the development of small towns.Such as much cultivated land lies idle,under-utilization and waste of land,increasing illegal use of land,unstable contractural relationship for land use.The relationship between the development of small towns and land use is also studied.Then the guidelines for the land system innovations of small towns are put forward.Namely the sustainability of social and economic development,the bal-ance between land reservation and land utilization,the provision of service to village ,agriculture and farmer,the manage-ment of land resource and land assets,the parsimonious andlegal use of land.The basic framework of land system innova-tions of small towns is put forward finally.It include the land replacement policy for small towns and the permanent tenan-cy of farmland,the overall plan for land uses and other plans in harmony,the establishment of a flexible system of land supplies,using land with payments ,the transfer of agricultural land and refining the land law related to the construction of small towns.     

青藏高原隆升的非线性动态有限元仿真研究

根据青藏高原的地质特征建立分析模型，采用3维动态有限元方法，在计算仿真板块速度场的基础上，计算在青藏高原的隆升过程中该地区地壳岩石的等效应力和位移随时间的变化，计算仿真得到的速度场与1998年GPS观测的速度场吻合良好；与过去一贯的假设相反，计算结果反映出地壳应力场不是静态的，而是此起彼伏，不断变化的，应力值最大且变化最剧烈的地区在克什米尔地区、鄂尔多斯地区和鲜水河－小江断裂带，与地震多发区域吻合。