Effects of 4-nonylphenol exposure in mussels (Mytilus galloprovincialis) and crabs (Carcinus aestuarii) with particular emphasis on vitellogenin induction

Since it is often difficult to estimate possible adverse effects due to contamination in selected ecosystems, multi-species biomonitoring may provide more information, taking into account different routes of exposure, ecological roles and metabolic capabilities of animals. In this context, we exposed for 7 days the mussel Mytilus galloprovincialis and the crab Carcinus aestuarii to 4-nonylphenol (NP), a well-known xenoestrogen. In mussels (0-0.2 mg NP l(-1)), we measured NP bioaccumulation in soft tissues and vitellogenin (Vg)-like protein levels in digestive glands from both males and females by the alkali-labile phosphate assay (ALP). As no reference data were available for crab exposure, the NP 96-h LC(50) value was previously determined. Then, in sublethally exposed (0-1.0 mg NP l(-1)) male crabs, NP bioaccumulation and Vg levels were measured in hemolymph, gonads and digestive gland. Bioaccumulation of NP increased from 43 to 371 microg g(-1) d.w. in mussels, and from 3.6 to 37 microg g(-1) d.w. in crabs, depending on the NP concentration in water. Dose-dependent Vg-like protein induction was observed in both species, appearing to be related to NP bioaccumulation, although a partial decrease was recorded at the highest concentration tested. A similar trend was observed in both digestive gland and gonad of exposed crabs; Vg increased to a lesser extent, although significantly, in hemolymph. Results demonstrated that NP induces Vg synthesis both in male and female mussels, as well as in male crabs. On the basis of the responsiveness of both species investigated, a multi-species approach is indicated in biomonitoring programmes.     

Immunotoxicity of the xenoestrogen 4-nonylphenol to the cockle Cerastoderma glaucum

The in vivo effects of 4-nonylphenol (NP) on functional responses of haemocytes from the cockle Cerastoderma glaucum were investigated after 7 days exposure to sublethal NP concentrations (0, 0+acetone, 0.0125, 0.025, 0.05 and 0.1 mg/l NP). Haemocytes from both controls and exposed cockles were collected, and the effects of NP on total haemocyte count (THC) and volume of circulating cells, intracellular superoxide anion (O(2)(-)) levels, acid phosphatase and lysozyme-like activities in both haemocyte lysate (HL) and cell-free haemolymph (CFH) were evaluated. Exposure of cockles to 0.1mg/l NP significantly increased THC (p<0.05) with respect to controls. Analysis of haemocyte size frequency distribution showed that the haemocyte fraction of about 7-8 microm in diameter and 250 femtolitres in volume increased markedly in cockles exposed to the highest NP concentration tested. Apoptosis resulting in cell volume reduction in NP-exposed animals cannot be excluded. No statistically significant variation in intracellular O(2)(-) levels was observed. Conversely, significant increases (p<0.05) in acid phosphatase activity were observed in CFH from 0.05 and 0.1mg/l NP-exposed animals; no significant differences in enzyme activity were recorded in HL. Lysozyme-like activity also increased significantly in CFH from cockles exposed to 0.05 mg/l NP (p<0.05) and 0.1 mg/l NP (p<0.001). Instead, lysozyme-like activity decreased significantly (p<0.05) in the HL of animals exposed to 0.05 mg/lNP. Our results suggest that NP induces variations in the functional responses of haemocytes of C. glaucum, mainly by reducing cell membrane stability and promoting cell degranulation.     

Lethal and estrogenic effects of 4-nonylphenol in the cockle Cerastoderma glaucum

The lethal and sublethal effects of the xenoestrogen 4-nonylphenol (NP) were evaluated in the cockle Cerastoderma glaucum. In a 96-h lethality test, bivalves were exposed to 0, 0+ acetone, 0.19, 0.38, 0.75, 1.5 and 3.0 mg NP/l. The 96-h LC(50) value was 0.3mg NP/l. No mortality was observed at 0.1 mg NP/l. The potential estrogenicity of NP was studied in both sexually undifferentiated (resting phase) and differentiated (pre-spawning phase) cockles, exposed for 7 and 14 days to 0, 0+ acetone, 0.0125, 0.025, 0.05, and 0.1 mg NP/l. Vitellogenin (Vg)-like protein levels were determined in both haemolymph and digestive gland by the alkali-labile phosphate (ALP) assay. In the resting phase, exposure for 7 days to 0.1 mg NP/l resulted in significant increases in ALP in both haemolymph and digestive gland, compared with controls. A significant increase was also observed in digestive gland of animals exposed to 0.0125 mg NP/l-exposed animals. After 14 days of exposure, haemolymph ALP levels were significantly increased in exposed animals at all NP concentrations tested, whereas no difference was recorded in digestive gland. In the pre-spawning phase, exposure for 7 days to NP significantly increased ALP levels in haemolymph from males exposed at all NP concentrations tested, whereas no significant variations were found in haemolymph from females. NP (0.05 and 0.1 mg/l) was also shown to increase ALP concentrations significantly in digestive gland of males, but not in those of females. Likewise, after 14 days' exposure, ALP levels significantly increased in haemolymph from males only at 0.1 mg NP/l. Conversely, NP caused significant increases in ALP levels in digestive gland from both males (at all NP concentrations tested) and females (at 0.025 and 0.1 mg NP/l). These results demonstrate that NP induces Vg synthesis in C. glaucum. Interestingly, males were more responsive to NP than females.     

Non-explosive magma–water interaction in a continental setting: Miocene examples from the Eastern Cordillera (central Andes; NW Argentina)

The Middle-Upper Miocene Las Burras–Almagro-El Toro (BAT) igneous complex within the Eastern Cordillera of the central Andes (∼24°S; NW Argentina) has revealed evidence of non-explosive interaction of andesitic magma with water or wet clastic sediments in a continental setting, including peperite generation. We describe and interpret lithofacies and emplacement mechanisms in three case studies. The Las Cuevas member (11.8 Ma) comprises facies related to: (i) andesite extruded in a subaqueous setting and generating lobe-hyaloclastite lava; and (ii) marginal parts of subaerial andesite lava dome(s) in contact with surface water, comprising fluidal lava lobes, hyaloclastite, and juvenile clasts with glassy rims. The Lampazar member (7.8 Ma) is represented by a syn-volcanic andesite intrusion and related peperite that formed within unconsolidated, water-saturated, coarse-grained volcaniclastic conglomerate and breccia. The andesite intrusion is finger-shaped and grades into intrusive pillows. Pillows are up to 2 m wide, tightly packed near the intrusion fingers, and gradually become dispersed in the host sediment ≥50 m from the parent intrusion. The Almagro A member (7.2 Ma) shows evidence of mingling between water-saturated, coarse-grained, volcaniclastic alluvial breccia and intruding andesite magma. The resulting intrusive pillows are characterized by ellipsoidal and tubular shape and concentric structure. The high-level penetration of magma in this coarse sediment was unconfined and irregular. Magma was detached in apophyses and lobes with sharp contacts and fluidal shapes, and without quench fragmentation and formation of a hyaloclastite envelope. The presence of peperite and magma–water contact facies in the BAT volcanic sequence indicates the possible availability of water in the system between 11–7 Ma and suggests a depositional setting in this part of the foreland basin of the central Andes characterized by an overall topographically low coastal floodplain that included extensive wetlands.     

On the Inclination Distribution of the Jovian Irregular Satellites

Irregular satellites—moons that occupy large orbits of significant eccentricity e and/or inclination I—circle each of the giant planets. The irregulars often extend close to the orbital stability limit, about 1/3-1/2 of the way to the edge of their planet's Hill sphere. The distant, elongated, and inclined orbits suggest capture, which presumably would give a random distribution of inclinations. Yet, no known irregulars have inclinations (relative to the ecliptic) between 47 and 141°.This paper shows that many high-I orbits are unstable due to secular solar perturbations. High-inclination orbits suffer appreciable periodic changes in eccentricity; large eccentricities can either drive particles with ∼70°<I<110° deep into the realm of the regular satellites (where collisions and scatterings are likely to remove them from planetocentric orbits on a timescale of 10⁷-10⁹ years) or expel them from the Hill sphere of the planet.By carrying out long-term (10⁹ years) orbital integrations for a variety of hypothetical satellites, we demonstrate that solar and planetary perturbations, by causing particles to strike (or to escape) their planet, considerably broaden this zone of avoidance. It grows to at least 55°<I<130° for orbits whose pericenters freely oscillate from 0 to 360°, while particles whose pericenters are locked at ±90° (Kozai mechanism) can remain for longer times.We estimate that the stable phase space (over 10 Myr) for satellites trapped in the Kozai resonance contains ∼10% of all stable orbits, suggesting the possible existence of a family of undiscovered objects at higher inclinations than those currently known.     

Acetylcholinesterase as a biomarker of exposure to neurotoxic compounds in the clam Tapes philippinarum from the Lagoon of Venice

Measurement of acetylcholinesterase (AChE) is widely used as a biomarker of exposure to neurotoxic compounds in aquatic environments. In the present study, AChE activity was monitored in the clam Tapes philippinarum in the Lagoon of Venice, with the aim of evaluating the possible exposure of animals to neurotoxic compounds. Two sampling strategies were followed. In the first case, temporal and spatial variations of AChE activity were measured in clams collected seasonally (from October 2003 to June 2004) in 3 sites of the Lagoon: Campalto (site 1), near a sewage treatment plant, Marghera (site 2), a highly polluted area, and Poveglia (site 3), a reference site. The condition index (CI) of clams was also calculated. In the second case, AChE activity was measured in clams collected at the end of seasonal samplings in 12 sites located throughout the Lagoon: areas licensed for clam farming (sites 5 to 11), nearby canals characterised by waste waters from agricultural areas (sites 4, 12, 13, 14), and a reference site outside the Lagoon (site 15). Results revealed a significant reduction in AChE activity in clams from Marghera in October, January and April, indicating their probable exposure to neurotoxic compounds originating in the industrial zone of Porto Marghera. A seasonal trend in AChE activity was also observed, enzyme activity being higher in January and lower in June. Clams from Campalto generally had higher CI values than those from Marghera and Poveglia. However, no particular correlation between AChE activity and CI was found. Lastly, marked differences in AChE activity were recorded when comparing enzyme activity of clams from various sites in the Lagoon of Venice with those of clams collected at site 15. Significant reductions in AChE activity were observed in animals collected in both nearby canals and licensed areas, indicating the homogeneous spatial distribution of potentially neurotoxic compounds throughout the Lagoon. It is probable that sediment mobilisation caused by clam harvesting and the passage of shipping plays an important role in resuspension of persistent contaminants having neurotoxic activity.     

Tethyan ophiolites and Pangea break-up

Abstract The break‐up of Pangea began during the Triassic and was preceded by a generalized Permo‐Triassic formation of continental rifts along the future margins between Africa and Europe, between Africa and North America, and between North and South America. During the Middle–Late Triassic, an ocean basin cutting the eastern equatorial portion of the Pangea opened as a prograding branch of the Paleotethys or as a new ocean (the Eastern Tethys); westwards, continental rift basins developed. The Western Tethys and Central Atlantic began to open only during the Middle Jurassic. The timing of the break‐up can be hypothesized from data from the oceanic remnants of the peri‐Mediterranean and peri‐Caribbean regions (the Mesozoic ophiolites) and from the Atlantic ocean crust. In the Eastern Tethys, Middle–Late Triassic mid‐oceanic ridge basalt (MORB) ophiolites, Middle–Upper Jurassic MORB, island arc tholeiite (IAT) supra‐subduction ophiolites and Middle–Upper Jurassic metamorphic soles occur, suggesting that the ocean drifting was active from the Triassic to the Middle Jurassic. The compressive phases, as early as during the Middle Jurassic, were when the drifting was still active and caused the ocean closure at the Jurassic–Cretaceous boundary and, successively, the formation of the orogenic belts. The present scattering of the ophiolites is a consequence of the orogenesis: once the tectonic disturbances are removed, the Eastern Tethys ophiolites constitute a single alignment. In the Western Tethys only Middle–Upper Jurassic MORB ophiolites are present – this was the drifting time. The closure began during the Late Cretaceous and was completed during the Eocene. Along the area linking the Western Tethys to the Central Atlantic, the break‐up was realized through left lateral wrench movements. In the Central Atlantic – the link between the Western Tethys and the Caribbean Tethys – the drifting began at the same time and is still continuing. The Caribbean Tethys opened probably during the Late Jurassic–Early Cretaceous. The general picture rising from the previous data suggest a Pangea break‐up rejuvenating from east to west, from the Middle–Late Triassic to the Late Jurassic–Early Cretaceous.     

First evidence of altered vitellogenin-like protein levels in clam Tapes philippinarum and in cockle Cerastoderma glaucum from the Lagoon of Venice

Possible xenoestrogenic effects were investigated, for the first time, in two bivalve species from the Lagoon of Venice (Italy): the clam Tapes philippinarum and the cockle Cerastoderma glaucum. Bivalves were collected far from their reproductive phase at the very early stage of gametogenesis (January), and in the pre-spawning period (June) in six sites. Vitellogenin (Vg)-like proteins (a biomarker of exposure to estrogenic compounds) were measured by the alkali-labile phosphate method (ALP), in both haemolymph and digestive gland from males and females (when it was possible to distinguish sex by microscopic observation of gonadal tissue). Haemolymph calcium concentrations (a parameter considered closely related to the presence of Vg-like proteins) and the bivalve condition index (CI) were also measured. In both seasons, bivalves collected at Campalto (near a sewage treatment plant) and Marghera (a highly contaminated area) had higher Vg-like protein levels, particularly in haemolymph, than animals from the other sampling sites. Interestingly, CI had high values in these polluted sites. In June only, Vg-like proteins and Ca²⁺ levels in haemolymph exhibited similar trends in both bivalve species at most sampling sites. The responsiveness of bivalves to environmental xenoestrogens was higher in June, allowing better discrimination among sites. The present study demonstrates that animals from highly polluted areas have increased Vg-like protein levels. As endocrine disruption due to exposure to estrogenic compounds may cause fertility reduction, alterations in the sex ratio, and a decrease in reproductive rate, a condition of potential risk for bivalve populations in estuarine areas is highlighted.