Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

泉州湾河口湿地鸟类的种类组成与分布

本文根据1988-1999年进行4次泉州湾河口湿地鸟类调查资料，研究了泉州湾河口湿地自然概况，首次报道了该区鸟类13目30科108种，指出泉州湾河口地区鸟类栖息地面临的威胁因素，建议建立泉州湾河口湿地保护区以保护该区的自然生态系统和鸟类资源。     

水下工程项目监理的任务与条件

结合近年来参与有关水下工程检测和项目监理的实践体验 ,从总结水下工程项目的基本特征着手 ,分析了水下工程项目监理的主要任务 ,并结合对于实施水下工程项目监理必备装备条件的探讨 ,介绍了国内外现有水下监测技术装备的开发应用状况     

引入差比关系法分析西北太平洋TOPEX/POSEIDON卫星高度计测高数据

TOPEX/POSEIDON(T/P)卫星高度计数据信息中存在周期成分混淆问题.对其中的一类混淆引入差比关系方法对混淆的分潮进行分离.卫星轨道交叉点资料包括升轨和降轨资料,资料量比沿轨点资料多1倍,经分析发现:在已有为期6a多的观测资料时间序列中,在沿轨处混淆的分潮如K₁和SSA在交叉点处不再混淆,可以直接分离.因此首先对交叉点资料进行调和分析.然后由交叉点的分析结果得到分潮间的差比关系,处理到相近的沿轨点处,从而得到沿轨点的调和常数.用引入差比关系方法,对西北太平洋海区6a多的T/P卫星高度计资料进行了潮汐分析,并与沿岸及岛屿验潮站资料进行了比较,所得结果较满意.     

冲绳海槽北部表层沉积物中的钙质超微化石及其环境特征

为探讨冲绳海槽表层沉积物中钙质超微化石的分布规律及其影响因素 ,对海槽北部槽坡区 ( 2 9°5 8.33′— 31°31 .2 3′N ,1 2 7°39.5 5′— 1 2 8°5 9.61′E) 34个表层样品进行了分析和研究。共发现 35种钙质超微化石 ,其中优势种为Gephyrocapsaoceanica、Emilianiahuxleyi和Florisphaeraprofun da ;G .oceanica、E .huxleyi等的百分含量变化和特征属种U .tenuis的出现 ,表明该区钙质超微化石组合面貌与东海外陆架相似 ,和东海及南海都属于北太平洋中央水团组合。G .oceanica的含量与海水营养盐存在一定的关系 ,但两者之间并不存在明显的正相关性 ;暖水分子U .irregularis、U .sibogae和O .fragilis的较高含量和冷水分子G .caribbeanica和Calcidiscusleptoporus的较低含量 ,进一步反映该区钙质超微化石的分布受到黑潮及对马暖流的影响 ,其中温度、盐度和表层海水结构性质 ,如海水透明度等因素可能为主要控制因素。     

Effects of chemical ecological adjustment and control experiment on phytoplankton community in the Aoshan Bay

There is a low nutrient level in the Aoshan Bay. In June 1999, the chemical adjustmentand control experiment was made in the Aoshan Bay. Following tracts investigation was carried out before the experiment and on the 1st, 2nd, 4th, 5th, 6th and 45th day/after the experiment. While the variance of amount of phytoplankton, the replacement of superior species and the species composition of phytoplankton were researched. The results show that the amount of phytoplankton in the Aoshan Bay rises gradually after the experiment. Ceratium macroceros Cleve of pyrophyta was the dominant species before the experiment, its dominant index was 37.7％. Six days after the experiment, its dominant index dropped to 17.6％ . Meanwhile the dominant index of Asterionella japanics Cleve rose from 7.1％ to 39.2％ , it became the first dominant species. Forty-five days after the experiment, the amount of phytoplankton in the Aoshan Bay was 5.15 to 137.32 times more than that in 1997.     

Nitrogen forms and decomposition of organic carbon in the southern Bohai Sea core sediments

Study on form characteristics of nitrogen in marine sediments is the primary method to research its biogeochemical cycling and nitrogen form characteristics in core sediments can reflect the process and results of early diagenesis in a certain degree. In this paper, Sequential extraction process in natural grain size was used for studying the existent forms of nitrogen in five core sediments of the southern Bohai Sea for the first time. Nitrogen was divided into two parts -transferable and fixed based on whether it could be extracted by the reagent. Distributions and early diagenesis of transferable nitrogen forms in the southern Bohai Sea were researched integratedly. Results indicate that IEF - N and OSF-N are predominant forms in transferable part in the studied core sediments. Contents of different nitrogen forms vary differently with depth, and have different diagenesis process. Decomposition constant of organic nitrogen (ON) and OC are about 15.51 × 10~(-3)a~(-1)and 4.79× 10~(-3)a~(-1) respective     

Sea surface height anomaly and geostrophic circulation variations in the South China Sea from TOPEX/POSEIDON altimetry

The sea surface height anomaly (SSHA) and geostrophic circulation in the South ChinaSea (SCS) are studied using TOPEX/POSE1DON (T/P) altimetry data. The SSHA, which is obtained after tidal correction based on the tidal results from T/P data, is predominated by seasonal alternating monsoons. The results reveal that the SSHA in the central part of the SCS is positive in spring and summer, but negative in autumn and winter. It is also found that the SSHA in the SCS can be approached with the sum of tidal constituents SA and SSA. The geostrophic circulations in the SCS are calculated according to sea surface dynamic topography, which is the sum of SSHA and mean sea surface height. It is suggested that the circulation in the upper layer of the SCS is generally cyclonic and notably western intensified during autumn and winter, while the western intensification is weak during spring and summer. It is also indicated that the Kuroshio intrudes into the northeastern SCS throuth the Luzon Strait in winter. But ther     

In situ determination on food consumption and ecological conversion efficiency of a marine fish species, Hyporhamphus sajori

The food consumption and ecological conversion efficiency of a species marine pelagic andsmall size fish, Hyporhamphus sajori, were determined by using in situ stomach content method presented by Eggers. The results showed that: (1) the fish was taken in food all day, so empty-stomach rate was very low, taking up about 4.5% of the total determined fish number. However, the fish still has significant daily feeding rhythm. A feeding peak was found 0:00 o'clock at night, but feeding level was always high in the daytime; (2) relationship between instantaneous food content in stomach and corresponding time could be described as S_t = a ·e ~(-b ·t). There was not significant difference of instantaneous gastric evacuation rate between two determinations, with average value being 0.13 × 10~(-2)g/ (g·d) (wet weight); (3) the daily food consumption tended to change in irregular waving form, with average value being (10.16×l0~(-2)±1.19×l0~(-2) g/ (g·d) (wet weight) or (55.56 × 10~(-2) ±6.51 × 10~(-2) kJ/     

渤海小型底栖生物的丰度和生物量

该文是渤海 1997年 6月、1998年 9月和 1999年 4月 3个航次小型底栖生物调查结果。结果表明 ,3个航次小型底栖生物的平均丰度分别为 :(2 30 0± 12 0 6 ) ind/ (10 cm2 )、(86 9± 5 10 ) ind/(10 cm2 )和 (6 32± 4 0 0 ) ind/ (10 cm2 )。平均生物量分别为 :(15 2 1± 6 34) μg(dwt) / (10 cm2 )、(72 5±35 4 )μg (dwt) / (10 cm2 )和 (5 17± 393)μg (dwt) / (10 cm2 )。共鉴定出 14个小型底栖生物类群 ,其中自由生活海洋线虫丰度占绝对优势 ,桡足类丰度居第 2位 ,这两个类群总和占小型底栖生物总丰度的 94 .8%～ 97.5 %。在生物量中所占比例列前 4位的类群依次为线虫、多毛类、桡足类、双壳类 ,加起来超过小型底栖生物总生物量的 80 %。小型底栖生物的 74 %分布于 2 cm以浅表层中。小型底栖生物的丰度和生物量在渤海海峡和渤海中东部较高 ,与环境因子的相关分析表明小型底栖生物的丰度与水深呈极显著的正相关 ,与沉积物的中值粒径呈显著的负相关