由偏顶蛤贝壳中稳定同位素剖面追溯海水温度记录

本文用在加拿大诺瓦·斯科舍省芬克湾采集到的两只活体偏顶蛤(Modiolus)贝壳上的氧同位素组成重建了过去十年间的底层海水温度记录,它也可作为一种独立的方法来确定贝壳上外生长线的沉积时间。由于对贝壳进行了密集采样(可多至每个贝壳沉积年份取15个样品),贝壳上的δ~(16)O变化反映了芬克湾底层海水的季节性变化。两只贝壳所得的温度剖面符合得很好,说明它们基本上是与真实的环境温度相对应的。本研究证明在海胆疾病爆发时底层海水的温度并没有异常升高,这说明疾病传播的关键因素是带病的生物体,而不是温度。同位素技术也说明了在贝壳外表面上可见的外生长线是年轮性的,它们是在每年冬季最低水温时沉积出来的。     

Population structure of Haliotis rubra from South Australia inferred from nuclear and mtDNA analyses

1Introduction ThemajorityofAustralia’sabalonefisheryex ports(5.135kt,worth＄216millionin2002～2003,ABARE2004)consistofblacklipabalone(HaliotisrubraLeach,1814).AssuchH.rubrais consideredasanimportantmarineresourcewithin Australia.Likemanyabalonespecieswor…     

A cDNA microarray technique applied for analysis of global gene expression profiles in tributyltin-exposed ascidians

To analyze global gene expressions, we constructed a cDNA microarray from a basal chordate, the ascidian Ciona intestinalis. Ciona is a cosmopolitan species and a genomic analysis of Ciona revealed that ascidians had approximately 15,500 protein-coding genes. Our "Ciona intestinalis cDNA chip version 1 (Ci cDNA chip ver. 1)" has arrayed 13,400 unique Ciona cDNAs. To establish a detection system for gene expression profiles in wild ascidians using a cDNA microarray, we analyzed gene expressions in the whole body of Ciona adults after exposure to 100 nM tributyltin (TBT) for 24 h. In our preliminary array data using Ci cDNA chip ver. 1, we found more than 200 genes that showed strong differential expressions. These genes encoded proteins that were concerned with stress response, detoxification, oxidoreduction reaction, biosynthesis, and catabolism. This, the first large cDNA microarray of this animal, should facilitate analyses of global gene expressions following exposure to TBT.     

Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.     

Impacts of stormwater discharges on the nearshore benthic environment of Santa Monica Bay

Although large loads of potentially toxic constituents are discharged from coastal urban watersheds, very little is known about the fates and eventual impacts of these stormwater inputs once they enter the ocean. The goal of this study was to examine the effects of stormwater discharges on the benthic marine environment of Santa Monica Bay. Sediment samples were collected across a gradient of stormwater impact following significantly sized storm events offshore Ballona Creek (a predominantly developed watershed) and Malibu Creek (a predominantly undeveloped watershed). Sediments offshore Malibu Creek had a greater proportion of fine-grained sediments, organic carbon, and naturally occurring metals (i.e., aluminum and iron), whereas sediments offshore Ballona Creek had higher concentrations of anthropogenic metals (i.e., lead) and organic pollutants (i.e., total DDT, total PCB, total PAH). The accumulation of anthropogenic sediment contaminants offshore Ballona Creek was evident up to 2 km downcoast and 4 km upcoast from the creek mouth and sediment concentrations covaried with distance from the discharge. Although changes in sediment texture, organic content, and an increase in sediment contamination were observed, there was little or no alteration to the benthic communities offshore either Ballona or Malibu Creek. Both sites were characterized as having an abundance, species richness, biodiversity and benthic response index similar to shallow water areas distant from creek mouths throughout the Southern California Bight. There was not a preponderance of pollution tolerant, nor a lack or pollution sensitive, species offshore either creek mouth.     

Can turbidity caused by Corophium volutator (Pallas) activity be used to assess sediment toxicity rapidly?

The standard toxicity test organism, Corophium volutator, exhibits a behavioural response to contaminated sediments that causes increased turbidity of overlying water. We quantify the effects of this response to an estuarine sediment spiked with copper and hydrocarbon contaminated sediments from an oil installation in the North Sea. Turbidity measured 24 h after the start of a toxicity test shows a strong relationship with contaminant concentrations and with mortality after 10 days. Turbidity measurements can therefore give a rapid indication of sediment toxicity, permitting a reduction in storage time of sediments to be used in dilution series and toxicity identification evaluation (TIE) tests, reducing the likelihood of contaminants degrading prior to testing.     

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。     

盐渍土壤的介电特性

采用Ｌ波段介电探针，对沙壤，砂质粘土和粘土三种不同性质的土壤作了详尽的实验室实验，结果显示，土壤的盐度同介电常数的实无关，而同虚部相关，并随盐度的增加而增加。还表明，实数部分更多的依赖于土壤的湿度，虚数部分取决地土壤的盐度，土壤的含钠性对介电常数的实部和虚部都无影响。本研究展示了Ｌ波段对土壤盐度的微波响应。同时说明，在湿壤的条件下，采用Ｌ波段，将盐壤从钠壤中分离出来的可能性。