坛紫菜中藻胆蛋白的性质与化学组成研究

坛紫菜(于1988年采自青岛太平角海区人工养殖筏上)的水溶性色素粗提物经过硫酸铵沉淀和羟基磷灰石(HA)柱层析后,分离出藻蓝蛋白(RPC)、藻红蛋白(RPE)和变藻蓝蛋白(APC)。在中性介质中,其吸收光谱和荧光发射光谱与文献报道基本一致;但在酸性(pH=3)或碱性(pH=12)介质中,吸收光谱较在中性介质中有明显改变,原有的荧光性质也消失。RPC和APC只分离到一种聚集体,但RPE有两种不同的聚集体。用SephadexG—100凝胶过滤方法测量藻胆蛋白的分子量分别为:RPC117000,APC112000,小分子RPE38000,大分子RPE232000。对三种藻胆蛋白的氨基酸分析的结果表明,三种藻胆蛋白中都是酸性氨基酸的含量大于碱性氨基酸的含量。     

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.     

波浪作用下潜标拖缆动力学分析

对三维潜标拖缆在规则波作用下的动力响应进行了研究,首先基于集中质量法给出了缆绳在规则波作用下的控制方程,然后提出了两种不同类型的自由端边界条件:质点型和艇型自由端边界条件,前者把拖体视为作三个自由度线运动的质点,而后者把拖体视为作六自由度运动的艇体,最后用有限差分法进行了数值研究。数值计算结果表明潜标拖缆在规则波的作用下,不仅作近似的简谐振动,还会在横向、垂向产生一个偏移量,该偏移量的大小随浪向角的变化而变化,另外受遭遇频率的影响,振动的幅值也随浪向角的变化而呈现不同的特征。     

Hydrocarbon Potential of Pre-cenozoic Strata in the North Yellow Sea Basin

The North Yellow Sea Basin ( NYSB ), which was developed on the basement of North China (Huabei) continental block, is a typical continental Mesozoic Cenozoic sedimentary basin in the sea area. Its Mesozoic basin is a residual basin, below which there is probably a larger Paleozoic sedimentary basin. The North Yellow Sea Basin comprises four sags and three uplifts. Of them, the eastern sag is a Mesozoic Cenozoic sedimentary sag in NYSB and has the biggest sediment thickness; the current Korean drilling wells are concentrated in the eastern sag. This sag is comparatively rich in oil and gas resources and thus has a relatively good petroleum prospect in the sea. The central sag has also accommodated thick Mesozoic-Cenozoic sediments. The latest research results show that there are three series of hydrocarbon source rocks in the North Yellow Sea Basin, namely, black shales of the Paleogene, Jurassic and Cretaceous. The principal hydrocarbon source rocks in NYSB are the Mesozoic black shale. According to the drilling data of Korea, the black shales of the Paleogene, Jurassic and Cretaceous have all come up to the standards of good and mature source rocks. The NYSB owns an intact system of oil generation, reservoir and capping rocks that can help hydrocarbon to form in the basin and thus it has the great potential of oil and gas. The vertical distribution of the hydrocarbon resources is mainly considered to be in the Cretaceous and then in the Jurassic.     

长期非平稳荷载调谐质量减振阻尼器优化设计

研究外荷载为长期非平稳随机过程。考虑长期荷载的特性 ,采用 1个概率谱密度函数来反映长期非平稳随机荷载及其特征 ;概率谱密度函数是基于大量的一般谱密度函数的统计特性获得。以延长结构的抗疲劳使用寿命为目标函数 ,提出了调谐质量减振阻尼器的优化设计方法 ,这在实际工程中有着极为广阔的应用前景。本文旨在从理论上发展长期非平稳随机荷载作用下调谐质量减振阻尼器的优化设计方法 ;文中采用长期波浪实测数据 ,给出了 1个数值算例说明整个设计过程。     

南极磷虾年龄组成的体长频数分布混合分析

本文依据１９８９／１９９０和１９９０／１９９１两个南极夏季在普里兹湾以北海域所获的拖网样品，应用分布混合分析方法，研究大磷虾种群的年龄结构。结果表明，种群内最多可能包括６个年龄：发年幼体，１＾＋，２＾＋，３＾＋，４＾＋和＾５＋龄虾。     

不同生长期坛紫菜多糖中组分含量的变化

于１９９０－１９９１年，运用＾１３Ｃ－ＮＭＲ和化学分析方法研究南方产和移植北方养殖的不同生长发育阶段坛紫菜多糖的组分变化，结果表明，随着藻体生长发育，坛紫菜琼胶中３，６－内醚－Ｌ－半乳糖和６－ＯＣＨ３－Ｄ－半乳糖的含量逐渐增加，而硫酸基的含量呈现先增加后减少的规律；北移坛紫菜琼胶中３，６－内醚－Ｌ－半乳糖的含量高于南方坛紫菜，而硫酸基和６－ＯＣＨ３－Ｄ－半乳糖的含量低于南方坛紫菜。     

海洋鱼类卵壳亚微结构的研究

在现代生物科学中,应用生物卵壳亚微结构的物种特异性进行分类研究已成为可行性现实。在海洋生物学研究中,曾发现海胆类未受精的卵壳层颗粒中即存在着眀显的物种特异性差别( L?nning,1967)。很多学者通过对鱼类卵细胞发生期间的亚微结构及卵壳形成的研究,证实在鱼类卵壳结构中亦存在特异性的种间差异,并认为这些差异与分类学、生物学及其区系分布有密切关系( Müller& Sterba,1963; Yamamoto,1963;G?tting,1966; Anderson,1967; Flügel,1967)。目前我国对鱼类卵壳( Egg chorin)结构的研究还鲜为人知,本文亦是首次通过对12种海洋经济鱼类受精卵的卵壳结构差别的探讨,为进行鱼类系统分类和系统发育研究提供科学依据。     

海水中液-固界面的台阶型动力学曲线 Ⅱ.液膜扩散-递进扩散-界面分级交换反应联合控制的复合模型理论

为定量解释海水中液-固界面的台阶型动力学曲线及其台阶的消长变化规律,在我们已提出的“液膜扩散和递进液膜扩散控制的复合模型”和“海水中液-固界面分级离子/配位子交换理论”基础上,吸取Crank的若干数学处理方法,进而提出适合于非稳态体系的“液膜扩散-递进扩散-界面分级交换反应联合控制的复合模型”。