Soil microbial community changes as a result of long-term exposure to a natural CO2 vent

The capture and geological storage of CO₂ can be used to reduce anthropogenic greenhouse gas emissions. To assess the environmental impact of potential CO₂ leakage from deep storage reservoirs on the abundance and functional diversity of microorganisms in near-surface terrestrial environments, a natural CO₂ vent (>90% CO₂ in the soil gas) was studied as an analogue. The microbial communities were investigated using lipid biomarkers combined with compound-specific stable carbon isotope analyses, the determination of microbial activities, and the use of quantitative polymerase chain reactions (Q-PCR). With this complementary set of methods, significant differences between the CO₂-rich vent and a reference site with a normal CO₂ concentration were detected. The δ¹³C values of the plant and microbial lipids within the CO₂ vent demonstrate that substantial amounts of geothermal CO₂ were incorporated into the microbial, plant, and soil carbon pools. Moreover, the numbers of Archaea and Bacteria were highest at the reference site and substantially lower at the CO₂ vent. Lipid biomarker analyses, Q-PCR, and the determination of microbial activities showed the presence of CO₂-utilising methanogenic Archaea, Geobacteraceae, and sulphate-reducing Bacteria (SRB) mainly at the CO₂ vent, only minor quantities were found at the reference site. Stable carbon isotopic analyses revealed that the methanogenic Archaea and SRB utilised the vent-derived CO₂ for assimilatory biosynthesis. Our results show a shift in the microbial community towards anaerobic and acidophilic microorganisms as a consequence of the long-term exposure of the soil environment to high CO₂ concentrations.     

The knotty structure of the HII dwarf galaxy F348

[OIII] images and blue spectra of the emission-line dwarf galaxy F348 are presented. In [OIII] light, the object contains two knots about 9” NE of the nucleus and one large extended knot 11” to the SW. The nuclear region is hundred times less luminous in emission-line light than the knots. Despite the presence of line intensity ratios [OIII]λ5007/Hβ > 3 the prior classification as a Seyfert-2 object cannot be upheld. This clinches an earlier suggestion by Veron-Cetty & Veron (1986). In particular, we show that the line spectra can be modeled with photoionization models employing stellar input continua. Also, the line luminosities of the extranuclear knots are typical for giant HII regions. There is neither evidence for tidal tails nor for high velocity differences between the knots. In addition, the linear arrangement of the knots does not support interaction. It rather suggests self-propagating star formation In this picture, the faintness of the nuclear region can be understood by an edge-on view. In addition, the nuclear starburst appears to be fading in contrast to the young extranuclear star formation regions. Within the scheme of Melnick (1987), F348 has to be classified as a multiple-system HII galaxy.