中国小型底栖生物研究的40年——进展与展望

基于2016年希腊第16届国际小型底栖生物会议资料及我国已有的信息,本文论述了国内外小型底栖生物的研究概况,着重介绍了我国该领域的主要进展,包括小型底栖生物的方法学、丰度与生物量的分布、海洋线虫和底栖桡足类的分类学、入侵植物种对线虫群落的影响、粒径谱和次级生产力、摄食生态学、实验室培养和生活史、环境检测、海洋线虫的分子生物学与系统演化、生态动力学-水层底栖耦合模型以及人才培养。依据国内外研究现状提出了今后的发展目标和应着重加强的研究方向。     

深海热液区管状蠕虫Ridgeia piscesae β型半乳糖凝集素的克隆与功能研究

Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp c DNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10–50°C and p H 5–10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards He La cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.     

池塘养殖和海上吊笼养殖仿刺参肠道菌群结构对比分析

实验采用平板分离及16S r DNA序列分析法研究池塘养殖和海上吊笼养殖仿刺参(Apostichopus japonicus)的前肠、中肠和后肠菌群结构并进行对比分析.结果显示,从海上吊笼养殖的仿刺参的肠道内分离鉴定的240株菌株分属于3个门、11个属和34个种.从池塘养殖仿刺参的肠道内分离鉴定的211株菌株分属于3个门、11个属和49个种.两种养殖模式仿刺参肠道菌群结构存在较大差异,且同一仿刺参肠道各部分之间的菌群结构也存在较大的差异.海上养殖仿刺参的肠道优势菌群为弧菌属(Vibrio)、Formosa属、希瓦氏菌属(Shewanella),池塘养殖仿刺参的肠道内优势菌群为弧菌属(Vibrio)、芽孢杆菌属(Bacillus)、喜盐芽孢杆菌属(Halobacillus).两个菌群共有菌株有7个种,存在于在不同的肠道部位.两种养殖模式比较发现池塘养殖仿刺参的肠道菌群的丰富度大于海上吊笼养殖的仿刺参,且池塘养殖仿刺参肠道内芽孢杆菌(Bacillus)等益生菌的占比较高,海上吊笼养殖仿刺参分离得到较多的弧菌属(Vibrio)细菌.本研究可为南方仿刺参人工养殖中潜在益生菌的筛选提供基础资料.     

祁连山干旱山地草地生物量对水分条件的响应

以祁连山排露沟流域干旱山地为研究对象,对海拔2 700~3 000 m典型草地群落的草本种类、高度和生物量等进行调查,并同步测定样地内的土壤水分,分析草地生物量随海拔高度的季节性变化特征以及草本生物量和土壤水分的关系。结果表明：（1）草地地上生物量平均值为135.36 g·m^-2,并随海拔升高呈先增加后降低的"单峰"变化模式,在海拔2 900 m时最高,为176.79±28.37 g·m^-2。地下生物量平均值为946.13 g·m^-2,并随海拔升高生物量呈递增趋势,在海拔3 000 m时最高,为1 301.19 ±68.24 g·m^-2。（2）草地地上、地下生物量在不同海拔高度间差异性显著（P<0.05）;该流域干旱山地草地根冠比在4.14~11.95之间变化。（3）在生长季5~9月份,干旱山地草地土壤含水量在9.23%~31.31%之间波动,平均值为14.94%。（4）草本地上、地下生物量与土壤平均含水量均呈显著正相关（P<0.05）,相关性系数分别为0.7784和0.7843。在不同海拔草地群落中,不同土层含水量对草地生物量的贡献不尽相同,但60 cm以上根系主要分布层内的水分对草地生物量具有重要的意义。     

Geochemistry,Zircon U–Pb Analysis,and Biotite 40Ar/39Ar Geochronology of the Maoling Gold Deposit,Liaodong Rift,NE China

The Maoling gold deposit is located in the southwestern part of the Liaodong rift, NE China, and has estimated reserves of 25 t. In this paper we present the results of an investigation into the geochronology and geodynamic mechanisms of igneous activity and metallogenesis within the Maoling gold deposit. New zircon U–Pb age data, biotite ⁴⁰Ar/³⁹Ar age data, whole‐rock geochemistry, and Hf isotopic analyses are presented in order to constrain the petrogenesis and mineralization of the deposit. Zircon U–Pb dating of the Wolongquan biotite monzogranite and Maoling biotite granite yielded mean ages of 194.0 ± 1.1 Ma and 196.1 ± 1.1 Ma, respectively. All the granites are characteristically high‐K calc‐alkaline, enriched in light rare earth elements and large ion lithophile elements, and depleted in high field strength elements, which is consistent with the geochemical characteristics of arc‐type magmas. The Hf isotope characteristics indicate that the granites formed by partial melting of early Paleoproterozoic crustal material. In addition, biotite ⁴⁰Ar/³⁹Ar dating indicates that the Maoling gold deposit formed at 188.9 ± 1.2 Ma, implying that the mineralization was related to both the granite intrusions. Taking into account previous data on S–Pb–O–H isotopes and our new age data, the Maoling deposit can be classified as an intrusion‐related gold deposit. Taking into account the geology of the study area and adjacent regions, we propose that the Maoling gold deposit and its associated granitic intrusions formed in a geodynamic setting that was dominated by subduction of the Paleo‐Pacific Plate beneath the Eurasian continent.     

准噶尔盆地东部中二叠统平地泉组具“斑状”结构热水喷流沉积岩的成因及地质意义*

准噶尔盆地东部中二叠统平地泉组(相当于芦草沟组)发育一套陆内裂谷背景下的湖泊相砂岩、泥岩、灰岩、白云岩、凝灰岩互层以及它们的过渡岩石,是区内最主要的烃源岩和储集层。近年来,在帐北断褶带、石树沟凹陷和吉木萨尔凹陷中二叠统平地泉组暗色泥岩、泥晶白云岩中发现了一类特殊的沉积岩,该类岩石发育类似火山岩的斑状结构。“斑晶”主要为粗晶白云石、方解石、黄铁矿及方沸石等,在岩心标本上呈“树枝状”或“雪花状”散布于基质中;基质则主要由泥晶白云石或泥质沉积物构成,富含有机质,发育水平层理及小型变形层理等。岩石学和矿物学分析表明,“斑晶”往往为粗晶方解石或白云石的集合体,也常见黄铁矿、方沸石与碳酸盐矿物共生,“斑晶”方解石发育环带,而白云石未见明显环带。电子探针分析表明“斑晶”方解石具有低镁、低铁及锶分布不均匀的特点,而“斑晶”白云石(FeO含量介于7.272%~11.086%之间)与“基质”泥晶白云石(FeO含量为1.027%)相比具有明显富铁的特点。流体包裹体分析表明“斑晶”方解石均一温度平均为180.68,℃,“斑晶”白云石均一温度平均为320.95,℃。这种特殊 “斑状”白云岩和“斑状”泥质岩很可能是湖底热液喷流作用的结果。当湖水沿深大断裂下渗至地下深处,与围岩发生物质交换并被加热后再沿断裂返回地表喷涌而出,热液流体携带的离子达到过饱和后就会析出方解石、白云石及黄铁矿等集合体,随热液的喷涌作用上升并破碎散落于湖底细粒沉积物内。“斑状”白云岩的发现对新疆北部中二叠统热水喷流沉积作用及该区油气的成因研究具有重要的意义。     

铁含量对滑石脱水动力学的影响及其地质意义

在常压下研究了2种不同铁含量滑石的原位X射线衍射高温脱水反应。选取粒径2~5μm的2种不同铁含量的滑石样品,在常压、空气氛围下进行了同步辐射原位X射线衍射脱水实验。实验结果表明,铁含量高的滑石脱水温度明显偏低,2个滑石样品在常压下发生明显脱水反应的温度相差达127℃以上。滑石在常压下的脱水动力机制为随机成核和生长机制,符合Avrami方程。将实验数据拟合Avrami方程得出:n=1.669。由实验结果可以推测,不同铁含量的滑石脱水深度可能有几十到上百千米的差别,研究铁含量与滑石脱水动力的相关性对于了解俯冲带浅-中源地震的成因机制具有重要意义。     

陕西泾阳南塬滑坡干湿黄土快速大剪切力学特性

借助于先进的多功能大型高速高压环剪试验机,针对陕西泾阳南塬滑坡中的饱和黄土、干燥黄土在快速大剪切位移条件下的力学响应特性进行了环剪试验研究。研究结果表明:1)在相同试验条件下,与干燥状态黄土相比,泾阳南塬黄土在饱和条件下抗剪强度指标降低。2)在连续快速剪切条件下,泾阳南塬滑坡饱和与干燥黄土均表现出应变软化特征,饱和黄土比干燥黄土的软化特征更加显著。3)在相同法向应力条件下,剪切速率对饱和和干燥黄土峰值强度均有显著影响,但两种状态下土体峰值强度随剪切速率变化特征不同。饱和黄土峰值强度随剪切速率的增大而增大,而干燥黄土峰值强度随剪切速率的增大而减小。4)在不同含水状态下,泾阳南塬滑坡黄土剪切力学特性变化规律具有不同的产生机理。其中,干燥黄土抗剪强度在连续剪切过程中主要受黏土颗粒间摩擦(阻)力、颗粒运移与定向排列以及剪切面形态的控制。与干燥黄土相比,饱和黄土峰值抗剪强度变化主要受土黏聚力的控制并受水的影响;土体内水的作用能够加剧其应变软化程度并可形成光滑平整的剪切面形态,进而对其峰后剪切应力变化产生影响。     

Lithofacies paleogeography mapping and reservoir prediction in tight sandstone strata: A case study from central Sichuan Basin,China

Sand-rich tight sandstone reservoirs are potential areas for oil and gas exploration. However, the high ratio of sandstone thickness to that of the strata in the formation poses many challenges and uncertainties to traditional lithofacies paleogeography mapping. Therefore, the prediction of reservoir sweet spots has remained problematic in the field of petroleum exploration. This study provides new insight into resolving this problem, based on the analyses of depositional characteristics of a typical modern sand-rich formation in a shallow braided river delta of the central Sichuan Basin, China. The varieties of sand-rich strata in the braided river delta environment include primary braided channels, secondary distributary channels and the distribution of sediments is controlled by the successive superposed strata deposited in paleogeomorphic valleys. The primary distributary channels have stronger hydrodynamic forces with higher proportions of coarse sand deposits than the secondary distributary channels. Therefore, lithofacies paleogeography mapping is controlled by the geomorphology, valley locations, and the migration of channels. We reconstructed the paleogeomorphology and valley systems that existed prior to the deposition of the Xujiahe Formation. Following this, rock-electro identification model for coarse skeletal sand bodies was constructed based on coring data. The results suggest that skeletal sand bodies in primary distributary channels occur mainly in the valleys and low-lying areas, whereas secondary distributary channels and fine deposits generally occur in the highland areas. The thickness distribution of skeletal sand bodies and lithofacies paleogeography map indicate a positive correlation in primary distributary channels and reservoir thickness. A significant correlation exists between different sedimentary facies and petrophysical properties. In addition, the degree of reservoir development in different sedimentary facies indicates that the mapping method reliably predicts the distribution of sweet spots. The application and understanding of the mapping method provide a reference for exploring tight sandstone reservoirs on a regional basis.     

Submersion simulation in a typical debris flow watershed of Jianzhuangchuan catchment,Loess Plateau

Debris flow is one of the most serious and frequent geological disasters that occur in the Loess Plateau. The outbreak of a debris flow is sudden, ferocious, swift, and destructive. The characteristics and mechanism of debris flow were explored in this study via survey, numerical simulation, and simulation analysis in a Loess Plateau area (Huangling County, Shaanxi Province, China). Numerical models and formulas corresponding to the occurrence and movement mechanism were established based on the HEC-RAS, HEC-GeoRAS, and SWAT results. The range of debris flow deposition was determined through capturing the debris flow free surface. A hydrological model and critical rainfall threshold were determined in order to provide technical support for debris flow forecasting in the Loess Plateau. The results suggest that 10-year floods do not submerge any portion of the basin. One village area was affected by the 100-year flood (total area of 0.648 km²) while four villages areas were submerged by the 1000-year flood (total area of 1.39 km²). The method presented here may provide a reliable scientific basis for mitigating loss due to debris flow hazards.