春季青藏高原感热对中国东部夏季降水的影响和预测作用

利用1980-2012年青藏高原中、东部71个站点观测资料、全中国756站的月降水资料、哈得来中心提供的HadISST v1.1海温资料以及ERA-Interim再分析资料,综合青藏高原的感热加热以及全球海温,研究了春季青藏高原感热对中国东部夏季降水的影响,并建立预报方程,探讨了青藏高原春季感热对中国降水的预报作用。结果表明,青藏高原春季感热与中国东部降水关系密切,青藏高原春季感热异常增强伴随着长江流域中下游同期降水增多,后期夏季长江流域整流域降水也持续偏多,华南东部降水偏少。春季青藏高原感热的增强与环北半球中高纬度的罗斯贝波列密切相关,扰动在北太平洋形成的反气旋环流向西南方向延伸至西北太平洋,为长江流域输送大量的水汽,有利于降水的发生。夏季,伴随着前期青藏高原感热的增强,南亚高压位置偏东,西北太平洋副热带高压（西太副高）位置偏西偏南,西太副高北侧为气旋式环流异常。在西太副高的控制下,华南东部降水减少;西太副高西侧的偏南气流为长江流域带来大量水汽,并与来自北部气旋式环流异常西侧的偏北风发生辐合,降水增多。青藏高原春季感热异常是华南和长江流域夏季降水异常的重要前兆信号。加入青藏高原春季感热后,利用海温预报的华南、长江流域夏季降水量与观测值的相关系数有所提高,预报方程对区域降水的解释方差提高约15%。      

天山北坡中东段天然草地光谱植被指数特征

据１９９２－１９９４年天山北坡中东段各种主要天然草地类型混合牧草群落光谱观测资料,牧草产量资料分析发现,天然草地牧草产量与光谱被指数都具有季节变化特征,呈现出低到高,再由高到低的变化规律;天然草地光谱植被指数可以较了地反映牧草产量的年际变化和不同类型天然草地的产量差异,由于降水等自然条件的影响,牧草产量的年份,光谱植被指数也高,牧草产量低的年份,光谱植被指数也低。     

人工神经网络及其在地震研究中的应用

简述了人工神经网络的发展历史,详述了Ｂ－Ｐ神经网络的基本原理,介绍了其在地震研究中的应用,文后对神经网络研究中应注意的问题进行了讨论。．     

海南岛泥蚶人工育苗的初步研究

根据海南岛的气候特点,采取了特定的技术措施,育苗用水加FeCl3处理、二次过滤;亲蚶排放时,单独设置作精池;附着基质选择非酸性海泥;培养耐高温的饵料。另外,还做了亲蚶和稚贝倒池培养实验,结果证明：12小时倒池一次,亲贝培养效果最好;2天倒池一次,稚贝培养效果最好。     

山东济宁金乡大蒜产地重金属在土壤—农作物中迁移转化特征研究

基于金乡县南部地区土地质量地球化学调查与评价项目,系统采集表层土壤和大蒜样品,对As、Cd、Cr、Cu、Hg、Ni、Pb、Zn共8种重金属元素全量及形态数据进行深入分析,研究发现,土壤重金属形态除Cd、Hg外以残渣态占绝对优势;重金属生物可利用系数与SOM、pH、CEC均呈负相关;大蒜植株不同部位对元素的富集特征具有明显差异性,As、Cd、Cr、Pb、Ni、Cu、Zn表现为须根>叶身>假茎>蒜头;蒜头Cd与土壤Cd全量呈显著性正相关,其余元素与对应土壤中全量相关关系不明显。     

降低交叉反应的鱼类病原菌检测抗体芯片初步研究

采用菌体吸附法对海豚链球菌(Streptococcus iniae)、迟缓爱德华氏菌(Edwardsiella tarda)、鳗弧菌(Vibrio anguillarum)、杀鲑气单胞菌(Aeromonas salmonicida)、荧光假单胞菌(Psedomonas fluo-rescens)和海洋分支杆菌(Mycobacterium marinum)6种养殖鱼类病原菌的兔抗血清进行吸附,以减小抗血清与其它菌株的交叉反应;吸附纯化后的抗体作为捕获抗体构建了病原菌检测抗体芯片。实验结果显示,与未经过吸附的纯化抗血清构建的抗体芯片相比,血清吸附实验能有效减少交叉反应,降低抗体的非特异性吸附,提高细菌检测的特异性。同时,以迟缓爱德华氏菌为例,采用吸附纯化后抗体制备的抗体芯片检测人工感染迟缓爱德华氏菌和自然发病的牙鲆,均观察到迟缓爱德华氏菌的特异性显色反应,得到了较理想的检测结果。本文结果说明优化后的病原菌检测抗体芯片可用于水产养殖鱼类常见的上述6种细菌性疾病的有效检测。     

Impact factors on distribution and characteristics of natural plant community in reclamation zones of Changjiang River estuary

To identify impact factors on the distribution and characters of natural plants community in reclamation area, with survey data from 67 plant quadrats in July 2009, soil properties data from 216 sampling points in April 2009, and TM (30 m) data in 2006, the composition and characteristics of natural plants community in different time of the Fengxian area in the Changjiang (Yangtze) River estuary were analyzed with two-way indicator species analysis (TWINSPAN), multivariate analysis of variance (MANOVA), detrended canonical correspondence analysis (DCCA) and canonical correspondence analysis (CCA). The results show that: 1) The plant communities in the reclaimed area are mainly mesophytes and helophytic-mesophytic transitional communities, showing a gradient distribution trend with the change in reclamation years. Species richness (MA), species diversity (H) and above-ground biomass also increase with the increase of reclamation years. Nevertheless, they appear to decline slightly in the middle and late reclamation period (> 30 years). 2) With the rise in land use levels, the changes in species richness and species diversity tend to increase at first and then decrease; species dominance (D), however, tends to decline; and above-ground biomass increases slightly. 3) The distribution of the plant community is mainly influenced by the following factors: land use levels (R = 0.55, p < 0.05), soil moisture (R = 0.53, p < 0.05), soil salinity (R = 0.43, p < 0.05) and reclamation time (R = 0.40, p < 0.05).     

Localization and dynamic expression of a 27.8 kDa receptor protein for lymphocystis disease virus infection in sea bass (<Emphasis Type="Italic">Lateolabrax japonicus</Emphasis>) tissues

Lymphocystis disease virus (LCDV) infects target cells by attaching to a 27.8 kDa receptor (27.8R) protein in flounder Paralichthys olivaceus, and anti-27.8R monoclonal antibodies (MAbs) have been developed. However, the 27.8R existence in tissues of sea bass (Lateolabrax japonicus) and its role in LCDV infection have remained unclear. In this study, the results of western blotting demonstrated that the same 27.8R was shared by flounder and sea bass. LCDV-free sea bass individuals were intramuscularly injected with LCDV, and viral copies were detected in tissues from 3 h post infection and showed a time-dependent increase during 9 days infection. Distribution and synthesis of 27.8R in sea bass tissues were investigated by using anti-27.8R MAbs as probes. It was found that 27.8R was distributed in all the tested tissues. The levels of 27.8R protein were highest in gill and skin, then a bit lowly in stomach, head kidney and heart, followed by spleen, intestine, blood cells, gonad and liver, and least in kidney and brain in healthy sea bass. Upon LCDV infection, 27.8R synthesis was up-regulated in each tissue, and higher in the tissues with higher LCDV copies. The 27.8R and LCDV were detected in some peripheral blood leukocytes but not in red blood cells. These results suggested that 27.8R was widely distributed in sea bass tissues, and it served as a receptor and correlated with tissue tropism of LCDV infection. Furthermore, leukocytes had the potential of being a LCDV carrier and were responsible for a systemic infection of LCDV in sea bass.     

Detection of lymphocystis disease virus in Japanese flounder Paralichthys olivaceus and other marine teleosts from northern China

We isolated a strain of lymphocystis disease virus (LCDV) from Japanese flounder (Paralichthys olivaceus) cultured in northern China. Based on published sequences of major capsid protein (MCP) gene of LCDV-cn (GenBank: AF126405), we designed two primer sets P1/P2 and P3/P4. We then used one-step or nested PCR and in-situ hybridization (ISH) to detect LCDV and identify the target tissues or cells in infected Japanese flounder. The PCR products were positive in purified viral supernatant, skin nodules, gut, gill, kidney, spleen, stomach, heart, and liver of Japanese flounder. We compared the DNA sequence with 14 MCP nucleotide sequences from GenBank, including Megalocytivirus (OFIV and RSIV), Iridovirus (CzIV and WIV), Ranavirus (TFV and FV3), and Lymphocystivirus (8 LCDV). Based on the alignment, we confirmed the PCR product was from Lymphocystivirus (GenBank accession number DQ279090 (LCDV-HD)). Using ISH, we noted the presence of LCDV in the skin nodules, gut, gill, spleen, stomach, and heart of spontaneously infected Japanese flounders. We successfully amplified LCDV fragments from Schlegel’s black rockfish (Sebastes schlegeli Higendorf), redwing sea robin (Lepidotrigla microptera Günther) and turbot (Scophthalmus maximus) using the one-step and nested PCR, suggesting the target genes can be widely detected in fish using this method.     

滇西腾冲地区勐连花岗岩体南段LA-ICP-MS 锆石U-Pb定年及其构造意义

高黎贡构造带属于冈底斯地块东缘,滇西高黎贡花岗岩是冈底斯构造活动重要的岩浆记录。对高黎贡构造带的勐连花岗岩体南段的蒲川花岗岩地球化学特征进行的研究表明,蒲川花岗岩主要属于钙碱性-高钾钙碱性花岗岩,并表现出较强的过铝质特点;稀土元素配分模式曲线均表现为向右陡倾的LREE富集模式,具较弱的Eu负异常;花岗岩具后碰撞的特征,形成于主碰撞之后地壳向后碰撞-陆内转换的构造环境。LA-ICP-MS锆石U-Pb定年表明,蒲川花岗岩形成于早白垩世（128.9Ma±2.4Ma）,年龄、地球化学特征与高黎贡构造带北部和中部一致,其形成可能是滇西怒江带碰撞作用的岩浆响应。