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21.
Zooplankton are an essential component of the marine and estuarine food chains. The ecotoxicological risk to zooplanktonic communities, estimated through the use of the biomarkers, can be used as an early warning signal of a potential alteration of the ecosystem health. The aim of this project was to evaluate the potential use of several biomarkers (esterases, mixed function oxidases, porphyrins) in zooplanktonic organisms. The acetylcholinesterase activity (AChE) was determined in homogenates of whole organisms of 10 different zooplanktonic crustaceans. Mean activity of AChE was 10.05 micromol/min/g for Acartia margalefi; 3.30 for Acartia latisetosa; 79.70 for Siriella clausi; 49.97 for Diamysis bahirensis; 7.48 for Siriella armata; 14.20 for Mysidopsis gibbosa; 4.49 for Euphausia crystallorophias; 1.66 for Euphausia superba; 2.74 for Streetsia challengeri; 13.26 for Meganycthiphanes norvegica. The species moreover showed a linear increase in enzyme activity in relation to the increase in sample concentration. The key result of this study concerns the different AChE activity basal values of the crustaceans' different species. 相似文献
22.
两株琼胶酶高产细菌的筛选和鉴定 总被引:4,自引:1,他引:4
从海洋环境中筛选得到两株琼胶酶的高产菌株,通过形态观察和生理生化反应,确定它们属于弧菌属,通过BIOLOG细菌鉴定系统鉴定,并同弧菌属标准菌株分析比较,确定上方宝剑两株菌都是塔式弧菌(Vibrio tubiashii),这两株菌在碳源利用方面存在差别。 相似文献
23.
Since 1998, a biomonitoring programme has been implemented to assess the potential impact of chemical mosquito control on macroinvertebrates of the coastal wetlands of Morbihan (Brittany, France). Acetylcholinesterase and carboxylesterases were used as biomarkers to assess the effects of Abate 500e (a.i. temephos) and Vectobac 12 AS (a.i. endotoxins of Bacillus thuringiensis var. israelensis, Bti) in Nereis (Hediste) diversicolor. Esterase inhibition revealed a marked impact of temephos, suggesting preferential contamination of the worms through the food. In Bti-exposed N. diversicolor, random variations of esterase activities were observed, that could not be attributed to the larvicide. However, esterases only reflected indirect physiological effects of Bti, and further investigations are needed to identify biomarkers more specific of Bti endotoxins. 相似文献
24.
Macrofauna impact on Ulva rigida C. Ag. production and relationship with environmental variables in the lagoon of Venice 总被引:2,自引:0,他引:2
The grazing pressure of the macrofaunal invertebrates associated with the biomass of Ulva rigida in the Venice lagoon, their species composition and relationship with environmental variables have been studied. Fifteen sampling campaigns were carried out during different seasons both in the central basin of the lagoon, dominated by macroalgae (especially U. rigida C. Ag.) and in the southern basin, prevalently populated by seagrasses (especially Zostera marina L.). Replicate experiments were conducted in the field by exposing Ulva fronds in net cages of 10 and 1 mm (control) mesh-sizes to allow or prevent grazer entrance. The grazing pressure was determined as Ulva growth rate difference in the cages. In the absence of invertebrate herbivores, Ulva exhibited per cent relative growth rates (%RGRs) ranging from 1.5 to 9.5% day(-1), whereas in their presence the %RGRs were significantly lower (from -2.5 to 3.4% day(-1)) and frequently negative, especially in the station dominated by macroalgae. In this area, peak grazing rates and macrofauna biomasses of up to 8.6% day(-1) and 1,480 g m(-2) fwt (84.4 ash-free dry weight), respectively, were found. On the whole, during in field experiments in the Ulva-dominated station, herbivores removed an amount of biomass whose percentage ranged from 59 to 165% (mean: 103%) of the biomass yield (grazers excluded) found in the cages. These results suggest the possibility that grazers could act as an important factor affecting Ulva production in the Venice lagoon. Macrofauna populations were analysed by means of multivaliate techniques applied to biological variables only and biological and environmental variables together. Data of individual abundance, after a log(x + 1) transformation and the calculation of the Bray-Curtis matrix, were classified using the Cluster Analysis and ordinated by means of the Non-Metric Multidimensional Scaling (MDS) technique, in accordance with the strategies used in the study of multispecies distributions. Finally, biological and environmental variables were analysed together by means of correlation matrices and the Principal Component Analysis. 相似文献
25.
Akcha F Tanguy A Leday G Pelluhet L Budzinski H Chiffoleau JF 《Marine environmental research》2004,58(2-5):753-756
DNA single-strand breaks were measured by the comet assay in both gill and hemolymph cells of mussels collected in 3 sampling areas of the French coast (Pointe du Castelli, Pen Bron and Saint-Nazaire Harbour). Whole mussel tissue samples were also collected for the chemical determination of PAH, PCB and heavy metal concentrations. In mussel, a higher level of DNA strand breaks was measured in gill than in hemolymph cells (p < 0.01). Despite a factor of contamination from 2 to 3 between sites, no difference in the extent of mussel DNA strand breaks was shown between sampling locations (p > 0.05), questioning the sensitivity of the assays used in biomonitoring studies. 相似文献
26.
27.
Micronucleus (MN) frequency is generally accepted as a marker of chromosomal damage and has been studied in a variety of cells and species. In previous work, we detected significant dose-related MN increases in the epithelial-like gill cells and agranular haemocytes of Mytilus galloprovincialis treated with benzo[a]pyrene, a well-known mutagenic pollutant. In addition, we have studied micronuclei and other nuclear abnormalities in mussels collected from the Venice lagoon (Italy). Frequency changes, possibly related to genotoxic/toxic stress, in both granular and micronucleated cells from gills and haemolymph, were detected. Environmental data suggest the effect of genotoxic pollutants and the importance of cell replication in the interpretation of micronucleus frequencies. 相似文献
28.
29.
M.R.Li B.Z.Bian 《山东海洋学院学报》1986,16(4):215-216
From a stock culture of Cylindrotheca sp., non-motile colony-forming cells were observed and isolated.Colony-forming cells produced both single cells and colony-forming cells in liquid medla.the rate of single cell production varied among clones. 相似文献
30.
23.1 介绍 模拟与频率和方向相关的大洋波谱F(f,θ)的工作是极其困难的。唯一有点统一的理论(Hasselmann,1967)也还没有简化成一种实用形式。这篇研究中所探讨的是结合零散的,现有的理论,以达到这样一种目的:即发展一种与我们目前对风浪成长谱的了解相一致 ·95.的波谱模式。本章中我们将讨论一种离散的谱模式,这种模式采用了一种改进的能量传播的特征方法。数值方法最早是由Barnett等(1969)提出的。 相似文献