Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products

cDNA clones for glutathione S-transferases A (GST-A) and A1 (GST-A1) from plaice (Pleuronectes platessa) were expressed as N-terminally 6XHis tagged proteins in Escherichia coli and purified to homogeneity from Ni-NTA silica. GST-A was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 micromol min(-1) mg(-1)). GST-A1 was a very efficient Se-independent glutathione peroxidase with an activity towards cumene hydroperoxide of 25 micromol min(-1) mg(-1). Although the enzymes exhibited moderately high activities towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) they exhibited little or no activity towards other common prototypical xenobiotic substrates. Together with data for ontogeny, tissue distribution and inducibility of these enzymes, we contend that a primary function of these enzymes is protection from the harmful effects of lipid peroxidation products generated naturally or exacerbated by xenobiotic exposure.     

多传感器岩心测试系统（MSCL）产品简介

多传感器岩心测试系统（MSCL系统）是Geotek公司用于岩芯测试的产品，能够在不同的环境下对沉积物、岩石和岩心进行快速、精确和自动的地球物理测试。系统的不同组合特性既可以使它在岸上实验室环境开展工作，也可以在科学调查船／钻探船上使用。为了满足不同客户的要求，GEOTEK公司开发了一系列不同的产品：1）标准型（MSCL—S）；2）垂直型（MSCL—V）；3）XYZ型（MSCL—XYZ）；4）保压型（MSCL—P）。根据MSCL系统的传感器组合，可以获得塑料衬管内整个岩心或分段岩心的地球物理数据。部分测量可以穿透铝质或钢质衬管，但塑料衬管一般来说比较好。根据不同的岩心类型和质量及不同的取样限制和测试要求，运用不同的MSCL系统组合具有明显的优势。     

天然气水合物钻探工作范围

1．0概要 含电缆测井的先导孔 （1）每个井场在电缆测井或取芯作业之前将钻探先导孔来确定进一步钻探和取芯作业的安全性。先导孔的钻探将使用位于井底总成（BHA）上的非返回式阀来防止高压泥浆及沉积物通过钻杆孔回返至海面。如果出现超压地层，还可使用TRW阀。     

Effects of prolonged exposure to 4-tert-octylphenol on toxicity and indices of oestrogenic exposure in the sand goby (Pomatoschistus minutus, Pallas)

Alkylphenolic compounds are present in estuarine and marine environments. They are moderately bioaccumulative, and oestrogenic to fish following three week, in vivo, exposures. The effects of prolonged exposure are, as yet, unclear. Sand goby (Pomatoschistus minutus) were exposed to measured concentrations of 4-tert-octylphenol (OP) in a 28-day, dose-ranging study, and a six month temporal response study. Following 28-days exposure to measured 31+/-6 or 101+/-47 microg l(-1) OP (mean+/-SD), immature male sand goby showed elevated vitellogenin (VTG) mRNA expression. Plasma alkali-labile phosphate concentrations were elevated in both sexes and were higher in females than males. Although measured OP concentrations were variable, they were significantly different for each treatment and a range of concentration-dependent effects were observed. The LC(50) for chronic OP exposure (8 weeks) was 29+/-6 microg l(-1), and both sexes demonstrated concentration and duration dependent increases in VTG mRNA expression. Exposure to 28+/-20 microg l(-1) OP (median+/-95% CI) for 6 months inhibited development of male nuptial colouration and sperm duct glands. These findings are discussed in relation to environmental concentrations of alkylphenols and ecological concerns.     

Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi

The role of endosymbiotic diatoms as pro-oxidant stressors in porifera has been investigated in the Antarctic sponge Haliclona dancoi in which the presence of diatoms is influenced by marked seasonal variations during the austral summer. Both chlorophaeopigments and frustules were absent in sponge tissues sampled in early November at the beginning of the summer and increased from the mid of December with slightly shifted temporal trends. The efficiency of antioxidant defenses in the sponge showed a marked response to symbionts with clearly enhanced values corresponding to the peak of diatoms.     

海洋微生物学的主要任务

海——微生物栖息的特殊环境。特殊的盐类成分,低温,高压,低浓度的有机物质,植物及动物区系的稀少等组成了微生物生命活动的广阔海洋区域一些主要生态特征。 海洋中最初的微生物学研究,早在上一世纪就已进行了,但足它们局限于较狭小的任务——     

太平洋麦哲伦海山区Roskomnedra平顶山地质构造特征与含矿性

1981年，法、美科学家用“太阳号”科考船在太平洋中部开展的工作，开始带动对海底山和平顶山富钴铁锰结壳的调查研究热潮。D．J．Frank等人关于多金属结壳适宜作为钴矿开采的观点，以及钴矿价格急剧增长引起的一系列政治问题就发生在这之前。     

Responses of a Mediterranean soft bottom community to short-term (1993-1996) hydrological changes in the Rhone river

The polychaete fauna of muddy bottoms off the Rhone delta (NW Mediterranean) was seasonally sampled at two sites at 70 m depth, from 1993 to 1996. During this period, five severe flood events occurred. A clustering analysis (distance coefficient of Whittaker) and three way fixed factor ANOVAs (site x season x year) showed strong year-to-year changes in species density and community structure, changes that masked seasonal variations. Total density increased by a factor of 3 and density of most species significantly increased following the first flooding event. Changes in the community structure were due to the successive dominance of a few species. The opportunistic species, such as Cossura sp., Mediomastus sp. and Polycirrus sp., exhibited peaks in density 1-3 months after the flood. These peaks were followed by a drastic decline. For species with a long life span, such as Sternaspis scutata, a slower but continuous increase in density was observed which was maintained for several months. The density fluctuations of these species exhibited a good correlation with river flow with time lags of 1-2 years. The successional dynamics observed are explained according to the geographical origin of the floods and the biology and feeding ecology of species.