北太平洋热带水体积的年际变化及其变化机制

利用EN4(the UK Met Office EN4.2.1 analyses)盐度数据发现北太平洋副热带高盐中心——北太平洋热带水(NPTW)的海表面积与体积在2000—2008年、2014—2017年存在下降趋势,2008—2014年期间存在上升趋势,进一步的研究表明,这些变化与太平洋年代际震荡(PDO)的位相转换紧密相关。利用淡水通量数据以及ECCO2(Circulation and Climate of the Ocean, Phase II)流场数据计算分析后表明,淡水通量对NPTW的变化贡献较小,而水平输运对NPTW的表面积以及体积变化贡献较大,这与PDO正(负)位相期间北赤道流(NEC)的向北(南)摆动有关。     

利用多源高度计资料提取南海低模态内潮能量

吕宋海峡由于剧烈变化的地形成为内潮产生的源地,内潮是海洋混合的重要原因。为了认知南海的内潮能通量分布,对南海的内潮有更好的理解,本文利用21世纪以来发射的多颗高度计卫星:J2、J1T、GFO以及EN,提取了吕宋海峡附近内潮的能通量。研究使用了调和分析和高通滤波等方法来提取第一模态内潮,主要提取K_1,K_2,M_2,N_2,O_1,P_1,Q_1和S_2八个分潮。同时结合WOA数据对能通量进行计算。结果表明,目标区域潮汐以全日分潮为主,所选区域的全日分潮中K_1所占比例最大;半日分潮中M_2分潮最强,而内潮的能通量则是M_2分潮所占最大,在吕宋海峡区域M_2能通量为6.45GW。内潮主要产生在地形变化剧烈的地方,海域的大部分地区内潮能量很小。在吕宋海峡中部,全日分潮能通量要小于南部地区,而半日分潮则有较大值。     

Differential gene expression in the body wall of the sea cucumber(Apostichopus japonicus) under strong lighting and dark conditions

Sea cucumber, Apostichopus japonicus is very sensitive to light changes. It is important to study the influence of light on the molecular response of A. japonicus. In this study, RNA-seq provided a general overview of the gene expression profiles of the body walls of A. japonicus exposed to strong light("light"), normal light("control") and fully dark("dark") environment. In the comparisons of "control" vs. "dark", "control" vs. "light" and "dark" vs."light", 1 161, 113 and 1 705 differentially expressed genes(DEGs) were identified following the criteria of|log_2 ratio|≥1 and FDR≤0.001, respectively. Gene ontology analysis showed that "cellular process" and "binding"enriched the most DEGs in the category of "biological process" and "molecular function", while "cell" and "cell part" enriched the most DEGs in the category of "cellular component". And the DEGs were mapped to 214, 41 and229 pathways in the Kyoto Encyclopedia of Genes and Genomes database, and 51, 2 and 57 pathways were significantly enriched, respectively. Light-specific DEGs identified in this study will be important targets for further investigation to establish the biochemical mechanisms involved in the adaption of this sea cucumber to changes in the level of environmental light.     

Sensitive and rapid detection of two toxic microalgae Alexandrium by loop-mediated isothermal amplification

A loop-mediated isothermal amplification (LAMP) assay was designed and evaluated for rapid detection of the toxic microalgae Alexandrium catenella and A. minutum, which can produce paralytic shellfish poisoning (PSP). Two sets of four specific primers targeting these two species were derived from the sequence of internal transcribed spacer (ITS) of ribosomal DNA. The method worked well in less than an hour under isothermal conditions of 65℃. LAMP specificity was validated in closely related algae as a comparison, suggesting the strict specificity of the LAMP primers. Two visual inspection approaches were feasible to interpret the positive or negative results. The detection limits of A. catenella and A. minutum samples using the LAMP assay were found to be 5.6 and 4.5 pg DNA, respectively. The sensitivity of this LAMP assay was 10 or 100-fold higher than Polymerase Chain Reaction (PCR) method in detecting the two microalgae. These characteristics of species specificity, sensitivity, and rapidity suggest that this method has the potentiality in the monitoring of red tide caused by A. catenella and A. minutum.     

济南城市四维动态地质信息系统构建研究

近20多年,济南泉水流量逐渐衰竭,泉水断流时有发生,为保护泉水,恢复泉水喷涌,塑造济南城市形象,该次研究基于50多年的济南地下水勘查、试验和研究成果,实现对城市多源、海量、多期次、多比例尺、异构地质数据的有效管理,然后建立不同地质数据的专业模型,实现对模型时空展布特征的三维可视化再现,并提供对模型的专业处理与分析。最后,从二维三维等水文地质概念模型出发,加入时间维度,由历史积累的时空关系,反映济南城市地下水的时空变化规律,在三维地质模型的基础上实现地下水动态监测数据的嵌入,构成地下水四维地质信息系统,在其空间表征的基础上,介绍了城市地下水的形成,泉水的分布、成因、演化以及泉水断流的原因、保泉供水的对策措施等,引导城市地下水的保护和发展,为政府决策提供参考。     

补肾生血方对肾性贫血大鼠EPO水平及EPOR mRNA的影响

目的：研究补肾生血方对肾性贫血大鼠促红细胞生成素（EPO）及EPOR mRNA水平的影响。方法：Wistar大鼠50只，分为对照组、模型组、中药组、EPO组、中药+EPO组，每组各10只，对除对照组外的其余组大鼠通过腺嘌呤灌胃制备肾性贫血大鼠模型，分别给予相应药物进行干预。分别于给药第2、8周观察血常规指标（Hb、RBC、HCT）、肾功能指标（BUN、Scr）的水平及EPO浓度，给药8周后处死大鼠，提取骨髓单个核细胞，测定EPOR mRNA表达量的变化。结果：与治疗2周相比，治疗8周中药组、EPO组、中药+EPO组大鼠的BUN、Scr显著降低，Hb、RBC、HCT、EPO水平及EPOR mRNA表达量显著上升，差异均有统计学意义（P＜0.05）。结论：补肾生血方具有改善肾性贫血模型大鼠肾功能、肾性贫血状况，其机制可能与提高骨髓有核细胞EPOR mRNA表达量，减轻毒素对EPOR的抑制，加强对内源性EPO反应性有关。     

Effects of feeding time on complement component C7 expression in Pelteobagrus vachellii subject to bacterial challenge

Journal of Oceanology and Limnology - Shifting the feeding time for fish from daytime to nighttime could alter their digestive behavior, disturb their metabolism, and may affect immune-related...     

Research and Test of Real-time Search Algorithm of Fast Radio Bursts Based on GPU Acceleration

In order to satisfy the research need of Nanshan 25 m radio telescope of Xinjiang Astronomical Observatory (XAO) and study the key technology of the planned QiTai radio Telescope (QTT), on the basis of the original CPU (Central Processing Unit)-based real-time search algorithm of FRBs (Fast Radio Bursts), we have studied the GPU (Graphics Processing Unit)-based real-time search algorithm of FRBs, and built the corresponding real-time search system of FRBs. We have made the comparison and analysis on the GPU system and CPU system, the test result shows that under a given searching accuracy, the speed of the GPU-accelerated algorithm is improved by 35–45 times compared with that of the CPU algorithm.     

波浪作用下沉积物中氮、磷释放速率的试验研究

湖泊或海湾中的沉积物吸附氮(N)、磷(P)等元素所形成的沉积层成为水体富营养化的內源。在波浪作用下,尤其是强浪作用下,沉积物中的N、P会通过内源释放作用大量进入水体中。本文在自制的U型水槽中开展实验,采用不同水头差来模拟波浪的循环荷载作用,研究了沉积层中的N、P在静置固结状态(Ⅰ)、加波未液化状态(Ⅱ)、加波液化状态(Ⅲ)下的释放规律,并给出该试验条件下的沉积物释放速率的拟合方程。试验结果表明:沉积物中总氮(TN)和溶解性总氮(TDN)的释放速率会随着水动力作用的增强而增加;总磷(TP)、溶解性总磷(TDP)和活性磷酸盐(SRP)的释放速率也随着水动力作用增强而增加,但水体中悬浮物(SS)含量过高会限制其释放速率。     

回阳生肌汤配合康复新液对肛瘘伴2型糖尿病患者术后创面愈合的影响

目的：观察回阳生肌汤配合康复新液对肛瘘伴2型糖尿病患者术后创面愈合的影响。方法：选择肛瘘伴2型糖尿病患者术后创面难以愈合的患者42 例，按照随机数字表法将其分为治疗组、对照组和空白组，每组各14 例。空白组予以常规换药及0.9%氯化钠注射液纱条外敷治疗，对照组在空白组基础上加服回阳生肌汤治疗，治疗组口服回阳生肌汤并以康复新液外敷治疗。观察3组患者术后第7、14、35天创面疼痛视觉模拟评分量表（VAS）评分、创面分泌物情况、创面愈合率及临床疗效。结果：总显效率治疗组为100.00%（14/14），对照组为85.71%（12/14），空白组为71.43%（10/14），治疗组优于对照组、空白组，差异有统计学意义（P＜0.05）；治疗组在术后第7、14、35天创面疼痛评分、渗液评分、愈合率方面均优于对照组及空白组，差异有统计学意义（P＜0.05）。结论：回阳生肌汤配合康复新液可促进肛瘘伴2型糖尿病患者术后创面愈合、减少创面分泌物，值得临床推广。