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1.
Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding. 相似文献
2.
自从在活动星系核NGC4945的视线方向上发现第一个河外H2O超脉泽源以来,迄今为止已发现了19个河外H2O超脉泽源,对与活动星系核成协和河外H2O超脉泽源及分子谱线的观测和研究是探测和研究活动星系核核区中央源,拱核气体和尘埃环性质的非常有效的工具,主要评述对河外H2O超脉泽源及与其相关分子谱线的搜索,观测和理论研究现状。 相似文献
3.
ZHANGPing-yu MAYan-ji YUZhen-han 《中国地理科学(英文版)》2002,12(4):315-320
Manzhouli is the largest land port city on the Sino-Russia border, transit cargo through the land port amount-ed to 5.95 million tons, transit tourists were 304 500 in 2000. It stands at the joint place of China, Mongolia and Russi-a, faces to Siberia area of Russia, receives direct support from the Northeast China and Bohai Sea Rim Area, and possess-es priorities in geographical location, land port infrastructure, water resources, coal resources, tourist resources andgreat potentiality in economic cooperation with Russia. The future urban function is a key port on the First Eurasia Continen-tal Bridge. Manzhouli Port will keep its first place between China and Russia land transport, and it is forecasted that thetransit amount through Manzhouli Port will go up to 10 million tons in 2005 and 20 million tons in 2010. It will be construct-ed to be a trade center of the peripheral area extending to Russia and Mongolia, a key export-oriented processing industri-al zone supported by industries such as export processing industries, export agriculture, trade services, technology trad-ing and the other service industries. It keeps being a well-known touring city for trade, shopping, sightseeing, vocation,local food, recreation and cultural events. To build Manzhouli Export Processing Industry Zone will improve city econom-ic structure, and the main sectors are organic food processing, livestock products processing, garment and furniture indus-try. Moreover, Manzhouli Export Processing Industry Zone will eventually be upgraded to be a border free trade zone.The city functional transition will inevitably affect urban spatial restructure and its expansion. The city space transforma-tion will develop as such: one development axis of No. 301 highway paralleling with Bin - Zhou(Harbin - Manzhouli) rail-way which cuts through central part of Manzhouli City, and links Zhalainuocr District with central city; three urban unitsincluding central city, Zhalainuocr District and Manzhouli Interchange Trade Zone; cohesion with Aoerjin and Cuogangpastures; regional dual-nuclei structure of Hailaer City and Manhzouli City; and the Manzhouli-Zabaykalsk Free TradeZone. 相似文献
4.
Grid method is employed for sampling covering soil at the test field,which is reclamation area filled by coal mining wastes for cropland in the Fushun coal mine,Liaoning Province,the Northeast China.The soil samples are taken at different locations,including three kinds of covering soil,three different depths of soil layers and four different covering ages of covering soil.The spatial-temporal variation of heavy metal element content in reclamation soil is stud-ied.The results indicate that the content of heavy metal elements is decreasing year after year,the determinant reason why the content of heavy metal elements at 60cm depth layer is higher than that at 30cm depth layer and surface is fertiliz-er and manure application;the metal elements mainly come from external environment;there is no metal pollution coming from mother material (coal mining wastes)in plough layer of covering soil. 相似文献
5.
A wide-field time-series CCD photometric survey of variable stars in the field of the open cluster NGC 2168 was carried out using the BATC Schmidt telescope. In total 13 new variable stars are discovered with three W UMa systems, one EA type and two EB type eclipsing binaries (one of them could be a W UMa system), and seven pulsating stars including three candidates of δScuti stars. 相似文献
6.
ENVIRONMENTAL SUSTAINABILITY AND SCENARIOS OF URBANIZATION IN ARID AREA——A Case Study in Wuwei City of Gansu Province 总被引:1,自引:0,他引:1
YOUFei LIYu DONGSuo-cheng 《中国地理科学(英文版)》2005,15(2):120-130
1IN T R O D U C T IO N The western China isfacinggrowing problems of eco- logicaland economic development. The disparitiebse- tween the socio-economy of the western and eastern China were enlarging, and the major ecologicalprob- lems stilelvolve as past(H… 相似文献
7.
Starfish oocytes with intact germinal vesicles (GVs) were cut along desired planes with glass needles or ligated using silk
thread loops into two parts and allowed to mature in vitro, and inseminated. The experimental results showed that (1) only
the parts with GVs or partial GV contents (PGVCs) cleaved, those without any GV materials did not; but nucleated and non-nucleated
fragments cut from mature eggs were able to divide; (2) the development of animal parts of oocytes containing GVs or PGVCs
was like that of animal fragments of matured oocytes with female pronuclei; most of them gave rise to permanent blastulae,
and just a few formed ectodermal vesicles with a little primary mesenchyme; (3) a large part of vegetal fragments with GVs
or PGVCs, and the vegetal parts of mature eggs without female pronuclei developed into small but normal embryos; (4) the fragments
containing GVs or PGVCs obtained from the oocytes along a plane parallel to the animal-vegetal (A-V) axis developed as normally
as the halves (with or without female pronuclei) severed from mature eggs along the same axis. Based on the data above, it
was concluded that (1) the non-chromatin materials in the oocyte GVs are indispensable for successful fertilization and cleavage
of starfish eggs; (2) some factor (s) located asymmetrically in the vegetal hemispheres of starfish oocytes is (are) responsible
for formation of the archenteron and primary mesenchyme. It is evident from the above findings that the oocyte cytoplasm of
the starfish had already regionalized before the GV break-down.
Contribution No. 1722 from the Institute of Oceanology, Academia Sinica 相似文献
8.
Methyl-galactosides were oxidized at room temperature by galactose oxidase in a one-step reaction and afforded methyl-galactoaldehyde
in excellent yield and high purity. The resulting galactoaldehyde as a useful intermediate can be directly used in glycopeptide
synthesis. 相似文献
9.
本文通过对1980年以来长江上游10次洪涝过程的500hpa环流形势分析,并用车比雪夫多项式对500hpa高度场进行展开,研究了各类洪涝过程的中期演变特征,发现代表不同洪涝过程环流形势变化的不同的车氏系数值都有明显的特征变化,结合欧洲中心中期数值预告96小时500hpa高度场的车氏系数分析,可为洪涝暴雨的中期预报提供一种新的思路。 相似文献
10.
Baolin Tan Haisheng Ji Guangli Huang Tuanhui Zhou Qiwu Song Yu Huang 《Solar physics》2006,239(1-2):137-148
Using one-minute cadence vector magnetograms from Big Bear Solar Observatory (BBSO), we analyze the temporal behavior of derived
longitudinal electric currents associated with two flares on July 26, 2002. One of the events is an M1.0 flare which occurred
in active region NOAA 10044, while the other is an M8.7 flare in the adjacent region 10039. Rapid changes of magnetic fields
in the form of flux emergence are found to be associated with both of these events. However, the temporal behavior of electric
currents are very different. For the M1.0 flare, the longitudinal electric current density drops rapidly near the flaring
neutral line; while for the M8.7 flare, the current density rapidly increases, confirming the picture of the current-carrying
flux emergence. We offer a possible explanation for such a difference: magnetic reconnection at different heights for the
two events, near the photosphere for the M1.0 flare, and higher up for the M8.7 flare. 相似文献