A Taxonomic Study of the Genus Tinocladia (Chordariales, Phaeophyta) in China Coasts I. Two new species, T. eudesmoides sp.nov. and T. zhangii sp.nov.

1INTRODUCTIONThegenusTinocladiaKylinderivedfromthege nusEudesmeJ.Ag.,wasestablishedbyKylinin1 940 .Untilnow ,fivespecieshavebeenreported .Threespecies,T .crassa (Sur.)Kylin(byC .K .Tseng,1 983 ) ,T .gracilisDingetLuandT .mi crosporangiiDingetLu (byDingetal.,2 0 0 4)inthegenusTinocladiahadbeenreportedtodate.Inthecourseofourinvestigationonthespecimensofthege nusTinocladiacollectedfromtheYellowSeacoastsandkeptintheHerbariumoftheInstituteofOceanolo gy,ChineseAcademyofSciences,twomo…     

THEORIES AND METHODS OF STRATEGIC ENVIRONMENTAL ASSESSMENT OF MODERN LOGISTICS DEVELOPMENT——A Case Study of Dalian City, China

1IN T R O D U C T IO N As a risingindustry,modern logisticpslaya vitalrolein thedevelopment ofurban economy . Meantime , many expertsin theworld are interestedin modern logistic.s Study on modern logisticsismostly focused on man - agement and program (HES…     

Effects of Body Weight and Water Temperature on Maximum Food Consumption of Juvenile Sebastodes fuscescens （Houttuyn）

Maximum rate of food consumption ( Cmax ) was determined for juvenile Sebastodes fuscescens (Houttuyn) at water temperature of 10, 15, 20 and 25℃. The relationships of Cmax to the body weight (W) at each temperature were described by a power equation: lnCmax = a b lnW. Covariance analysis revealed significant interaction of the temperature and body weight. The relationship of adjusted Cmax to water temperature (T) was described by a quadratic equation: Cmax = -0.369 0.456T - 0.0117T^2. The optimal feeding temperature calculated from this equation was 19.5 ℃. The coefficients of the multiple regression estimation relating Cmax to body weight (W) and water temperature (T) were given in the Table 2.     

ENVIRONMENTAL SUSTAINABILITY AND SCENARIOS OF URBANIZATION IN ARID AREA——A Case Study in Wuwei City of Gansu Province

1IN T R O D U C T IO N The western China isfacinggrowing problems of eco- logicaland economic development. The disparitiebse- tween the socio-economy of the western and eastern China were enlarging, and the major ecologicalprob- lems stilelvolve as past(H…     

The influence of Fe~(2+) on growth and development of cells enzy-matically isolated from Porphyra yezoensis blades

1 INTRODUCTION Porphyra (also called nori in Japanese) is one of the most important algae in aquaculture in the world. In the early 1980’s, basic studies on cell and protoplast isolation, cultivation and “seedling” breeding of Porphyra were conducted in laboratory. Zhao and Zhang (1981) isolated and cultured isolated cells by grinding the nori thallus, and Lu (1983) isolated cells by digesting bacteria. An enzyme isolated from a sea snail, was used to isolate cells and protoplasts (T…     

SUSTAINABLE URBAN-RURAL RELATION IN RAPID URBANIZATION AREAS——Case of Transformation of "Urban Village" in Guangzhou

1IN T R O D U C T IO NDue to acceleratingurban sprawl and suburbanizatio,nlargeareaoffarmlandwere changed intonon-agricultur-allanduse sincethelate1990s,especiallyinSouth Chi-na.Meanwhile some villageswere surrounded by urbanbuilt-upareasand became"thevillagesin urban area",which arenamed hereas"urban villages."Specificallyindefinitio,nurban villageisa ruralcom munity inurbanbuilt-upareasbecause of drasticurban sprawl,subur-banizationand industriadlispersion.A nd inrealityur-ban villagee…     

Effects of logging intensity on structure and composition of a broadleaf-Korean pine mixed forest on Changbai Mountains,northeast China

In order to identify a harvesting model which is beneficial for broadleaf-Korean pine mixed forest(BKF) sustainability, we investigated four types of harvested stands which have been logged with intensities of 0(T0, control), 15%(T1, low intensity), 35%(T2, moderate intensity), and 100%(T3, clear-cutting), and examined the impacts of logging intensity on composition and structure of these stands. Results showed that there were no significant differences between T0 and T1 for all structural characteristics, except for density of seeding and large trees. The mean diameter at breast height(DBH, 1.3 m above the ground), stem density and basal area of large trees in T2 were significantly lower than in T0, while the density of seedlings and saplings were significantly higher in T2 than in T0. Structural characteristics in T3 were entirely different from T0. Dominant tree species in primary BKF comprised 93%, 85%, 45% and 10% of the total basal area in T0, T1, T2 and T3, respectively. Three community similarity indices, the Jaccard′s similarity coefficient(CJ); the Morisita-Horn index(CMH); and the Bray-Curtis index(CN), were the highest for T0 and T1, followed by T0 and T2, and T0 and T3, in generally. These results suggest that effects of harvesting on forest composition and structure are related to logging intensities. Low intensity harvesting is conductive to preserving forest structure and composition, allowing it to recover in a short time period. The regime characterized by low logging intensity and short rotations appears to be a sustainable harvesting method for BKF on the Changbai Mountains.     

Partial Sequence Analysis of Mitochondrial COI Gene of the Chinese Shrimp, Fenneropenaeus Chinensis

Eight hundred and thirty eight base pair fragment of mitochondrial COI gene of wild and cultured populations (CP1, CP4, CP5 and CP6) of Fenneropenaeus chinensis was amplified and sequenced. The A, T, G and C contents of the sequence were 235bp (28.0% ),.307bp (36.6%), 138bp (16.5%) and 158bp (18.9%), respectively. Furthermore, 556bp fragment of the sequence was used to discuss the phylogenetic relationship among 14 Penaeidae species using Alpheus armillatus as the outgroup. From the molecular phylogenetic tree constructed by neighbor-joining method, we obtained three large shrimp groups: Farfantepenaeus, Litopenaeus and Fenneropenaeus group. The results also indicated that there were a closer genetic relationships between F. aztecus and F. paulensis, L. schmitti and L. setiferus, F. indicus and F. merguiensis, and the genus Farfantepenaeus was closer to Litopenaeus.     

Expression of the Phycoerythrin Gene of Gracilaria lemaneiformis (Rhodophyta) in E. coli and Evaluation of the Bioactivity of Recombinant PE

Phycoerythrin (PE) is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX (pGEX-PE) and BGL (pBGL). PE expressing in BEX (pGEX-PE) was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bod- ies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX (pGEX-PE) cells were col- lected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indi- cated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein con- centration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were significantly higher than the negative control BL21(pGEX-4T) (P<0.02), and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mg mL-1 BGL (pBGL) products, the scavenging effects of the supernatants of BEX (pGEX-PE) and BGL (pBGL) cell lysates were stronger than that of negative control BL21(pGEX-4T). However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially de- creased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This re- search showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.     

Two New Species of the Genus finocladia Kylin （Chordaria-les, Phaeophyta） from Yellow Sea Coasts of China

Two new species of the genus Tinocladia Kylin, T. gracilis sp. nov. and T. microsporangii sp. nov,, are described. T. gracilis is characterized by its slender frond, variable size, infrequently branched medullary filaments, and short assimilating filaments with 2 - 7 cells. T. microsporangii is characterized by its medullary cells which are slightly larger than sub-cortical ones; narrow sub-cortical layer, usually consisting of 4- 6 cells and very small unilocular sporangia. The holotypes are deposited at the Herbarium of the Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.     

A Taxonomic Study of the Genus Tinocladia （Chordariales,Phaeophyta） in China Coasts I. Two new species, T. eudesmoides sp.nov, and T. zhangii sp.nov.

Two new species of the genusTinocladia Kylin (1940)T. eudesmoides Ding et Lu sp. nov. andTinocladia zhangii Ding et Lu sp. nov., are described.T. eudesmoides is mainly characterized by its longitudinally and transversely produced sub-cortical filaments from the medulla, its short uniseriate assimilating filaments, usually composed of 6–10 cells with slightly swollen and narrow ultimate cells, and its unilocular sporangia (60–115)×55–70μm, variable in size.T. zhangii is mainly characterized by its very solid frond, not rip apart by hands, its evident longer primary branches, its short and slender secondary branches, with very evident sub-cortical layer, and filaments divided repeatedly, and its large and small unilocular sporangia, with the latter borne on an evidently swollen pedicel. Project B80981816 supported by the NSFC. Contribution No. 4443 from the Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.     

Three new species of Batrachospermum Roth (Batrachosper-maceae,Rhodophyta) in China

1 INTRODUCTION The first published records of genus Batra-chospermum Roth in China are from Jao (1941), when eleven taxa were reported. Since then, Shi, Hu and Kumano (1993), Shi (1994a, 1994b), Hua and Shi (1996), Xie et al. (1999, 2003), Xie and Shi (2003) have described in more detail the species from China. To date, 23 taxa of Batrachospermum Roth in China have been reported, of which, B. yunnanense, B. nothocladoideum and B. transito-rium are new species of this genus, and are…     

Three new species of Batrachospermum Roth （Batrachospermaceae, Rhodophyta） in China

Three new species of Batrachospermum Roth (Batrachospermales, Rhodophyta) from China are described in this paper. B. yunnanense sp. nov. has long-cylindrical trichogynes with long stalks and is diagnostic of section Virescentia. Within this section, B. yunnanense is similar to B. helminthosum Bory emend. Sheath et al., but it is dioecious and has curved carpogonial branches, while the latter is monoecious and has straight carpogonial branches. It is also similar to B. transtaganum Reis, but it differs from the latter in long carpogonia,big carposporophytes and carposporangia. It is considered that B. nothocladoideum sp. nov. is assigned to section Contorta, subsection Kushiroense, because its carpogonial branches are twisted and gonimoblast filaments are loosely agglomerated. This new species similar to B. iriomotense Kumano, but with short fascicles, long-ovoid or subpyriform cells, numerous terminal hairs, long-ellipsoid trichogynes, big carposporophytes and small carposporangia. The plant is quite tough and cartilaginous and similar to Nothocladus in gross morphology, but its carposporophytes are compact instead of diffuse. This shows that it may be a transitional species between section Contorta and genus Nothocladus. So, B. transitorium sp. nov. should belong to section Contorta, subsection Kushiroense, because of its curved or twisted carpogonial branches and loosely agglomerated gonimoblast filaments, with globose or subglobose cells in fascicles similar to B. spermatiophorum Vis et Sheath, but no colourless spermatiophores. In terms of small and numerous carposporophytes, B. transitorium sp. nov. is similar to some species of section Batrachospermum. However, their other features are unique, indicating its transitional nature between section Contorta and Batrachospermum.     

Descriptions of three new species of Mitridae （Mollusca： Gastropoda） from South China Sea

Three new species of Family Mitridae （Mollusca： Gastropoda） from the South China Sea are described in the present paper. They are Ziba aglais sp. nov. B. LI ＆ S. ZHANG, Neocancilla daidaleosa sp.nov. B. LI ＆ X. LI, and Mitra holkosa sp. nov. B. LI. Their systematic positions are also discussed.     

Two New Species of Free-Living Marine Nematodes of the Desmodoridae from Mangrove Wetlands of Xiamen Bay,China

Mangroves are unique in their biodiversity,but studies on their meiobenthic biodiversity in China are scarce.Despite the importance of mangroves,little work has been done on the classification of nematodes in mangrove wetlands.Fujian Province is the most northern point of China’s natural mangrove distribution,and it is also one of the provinces with the earliest constructed mangrove forest.In this paper,two new free-living marine nematode species of Desmodoridae from the Xiamen mangrove wetlands in China are described.Metachromadora xiamenensis sp.nov.is characterized by a cylindrical body,and smooth head capsule set off from the rest of the body.The cuticle is finely annulated and thickened at the midbody.Lateral ridges run from the posterior end of the pharynx to the middle of the tail.The amphid foveae is loop shaped and opens at the top with a double contour amphidial,pharynx with bipartite cuticularized internal cavity.There are 18 tubular precloacal supplements and tail with three small protuberances.Molgolaimus euryformis sp.nov.is characterized by a relatively short and plump body with finely annulated cuticle,which is particularly obvious in the tail.The head is small and wide with intensive striates.The inner and outer labial sensilla are indistinct with short spicules and ventral apophysis,a gubernaculum with a block-shaped hook,a swollen conical-cylindrical tail and an absence of precloacal supplements.     

Tritonia iocasica sp.nov.,a new tritoniid species from a seamount in the tropical Western Pacific(Heterobranchia:Nudibranchia)

During an expedition to a seamount at Caroline Plate in the tropical Western Pacific,a new species of Tritonia was sampled from the upper bathyal zone at depth of 970-1 262 m.This new species,Tritonia iocasica sp.nov.,represents the first tritoniid nudibranch known to feed on octocoral of the family Melithaeidae.The species is up to 120 mm in length,pinkish in color;with the rhinophoral sheath divided into several petaliform extensions;veil with,about 18 elongate digitiform processes;notal margin with17-19 pairs of secondary gills;anus located below the 5~(th) and 6~(th) secondary gills,and the genitalia below the 3~(rd) secondary gill on the right side of the body.Based on these external features,T.iocasica sp.nov.can be dearly distinguished from all previously described members of the genus.Phylogenetic analyses of two mitochondrial(COI,16 S rRNA) and a nuclear(H3) genes using Bayesian inference,maximum likelihood,and species delimitation analysis also support the separation of T.iocasica sp.nov.from its congeners.     

辽宁早侏罗世化石木材

辽宁地区木化石丰富，现已发现早侏罗世松柏类硅化木材 5属 8种，其中包括 2个新种， 2个修订种， 3个已知种及 1个未定种，即： Protophyllocladoxylon chaoyangense Zhang et Zheng sp. nov.,P. franconicum Vogellehner, Protosciadopityoxylon liaoxiense Zhang et Zheng sp. nov., P. jeholense (Ogura) Zhang et Zheng comb. nov., Sciadopityoxylon heizyoense(Shimakura) Zhang et Zheng comb. nov., Protocedroxylon dibneri (Shilkina) Shilkina et Chudajb., Xenoxylon latiporosum Gothan, X.? sp.。本文对新种作了描述，对前人鉴定有误的属种进行了修订，并概述了 5个产地木材化石组合特征及其古气候环境。     

<Emphasis Type="Italic">Grateloupia ramosa</Emphasis> Wang &amp; Luan sp. nov. (Halymeniaceae,Rhodophyta), a new species from China based on morphological evidence and comparative <Emphasis Type="Italic">rbc</Emphasis>L sequences

Grateloupia ramosa Wang & Luan sp. nov. (Halymeniaceae, Rhodophyta) is newly described from Hainan Province, southern China. The organism has the following morphological features: (1) purplish red, cartilaginous and lubricous thalli 5–10 cm in height; (2) compressed percurrent axes bearing abundant branches with opposite arrangement; (3) claw-like apices on top, constricted to 2–4 cm at the base; (4) cortex consisting of 3–6 layers of elliptical or anomalous cells and a medulla covered by compact medullary filaments; (5) reproductive structures distributed throughout the thallus, especially centralized at the bottom of the end portion of the branches; and (6) 4-celled Carpogonial branches and 3-celled auxiliary-cell branches, both of the Grateloupia-type. The morphological diff erences were supported by molecular phylogenetics based on ribulose-1, 5-bisphosphate carboxylase/oxygenase (rbcL) gene sequence analysis. There was only a 1 bp divergence between specimens collected from Wenchang and Lingshui of Hainan province. The new species was embedded in the large Grateloupia clade of the Halymeniaceae. The pairwise distances between G. ramosa and other species within Grateloupia ranged from 26 to 105 bp, within pairwise distances of 13–111 bp between species of the large genus Grateloupia in Halymeniaceae. Thus, we propose this new species as G. ramosa Wang & Luan sp. nov.     

Lower Kungurian shallow-water lagoon biota of Middle Cis-Urals,Russia:towards paleoecological reconstruction

The paper deals with the Lower Kungurian( Philippovian Horizon; Lower Permian) biota of the Middle Cis-Urals,Russia.The biota is characterized on the basis of four representative localities( Philippovskoe Bus-stop,Philippovskian Quarry,Kamai and Kiselevo-Suksun Highway),which are situated in the stratotype area of the Kungurian stage.All the localities can be regarded as Lagersttten,but of authentically low biodiversity,since they represent oligotrophic lagoons of abnormal fluctuating salinity.General reconstruction of the Philippovian near-shore shallow-water ecosystem is given.Two new species of algae Algites philippoviensis sp.nov.,A.shurtanensis sp.nov.,and a new species of the limulids Paleolimulus kunguricus sp.nov.are described for the first time.