Studies on immunodiagnosis of hepatopancreatic parvo-like virus diseases ofPenaeus orientalis

This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results. This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp. The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms.     

The prediction of penaeid shrimp yield in the Bohai Sea in autumn

This project was designed to predict penaeid shrimp yield in the Bohai Sea in autumn by means of multiple regression equations based on the data of abundance index obtained from the trawl surveys in the main spawning ground of shrimp in the Bohai Sea and the nursery grounds of juvenile shrimp (Bohai, Laizhou and Liaodong bays and the Luanhe River estuary) in early August every year from 1965–1982. The prediction method and its use are presented in this paper. The abundance indexes obtained from the four sea areas as four independent variables, when subjected to an F test, showed that they had significant effects on the penaeid shrimp yield in the Bohai Sea in autumn.     

STUDIES ON IMMUNODIAGNOSIS OFHEPATOPANCREATIC PARVO- LIKEVIRUS DISEASE OF THE CHINESEPENAEID, PENAEUS CHINENSISI. PURIFICATION OF THE VIRUS

The viral disease of penaeids is indistinct but highly dangerous because their symptoms are often masked by some secondary ones.In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV)disease of cultured Penaeus chinensis was conducted using immuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar.     

On the 2-crop culture of penaeid shrimps

Although much progress has been made in the last few years on the hatchery rearing of penaeid shrimps, the use of artificial feeds and the innovation of culture techniques, the key problem in the shrimp culture industry is still to increase yield per unit culture area for greater economic returns. In finding an answer to this problem, experiments on the cultivation of two (instead of one) species with different biological requirements were carried out in different seasons,Penaeus orientalis Kishinouye in spring andP. penicillatus Alcock in summer in Fujian as it has a longer culture season. Years of study and practical experience in large-scale rearing of shrimps show that successive 2-crop culture of penaeid shrimps is feasible and more economical as no additional investment in terms of maintenance facilities and management is needed. The pond is fully used the whole year. The renovation of techniques and the timing of the culture of the two species led to the development of a 2-crop culture method which roughly doubled the yield per unit area of cultivation. The early season species is harvested at the prime of its development and the culture of the late season species begin just after the harvest of the early season species.     

Studies on immunodiagnosis of hepatopancreatic parvo-like virus disease of the Chinese penaeid,Penaeus Chinensis I. Purification of the virus

The viral discase of penaeids is indistinet but highly dangerous because their symptoms are often masked by some secondary ones. In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV) disease of culturedPenaeus chinensis was conducted using ummuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar.     

一种抗简单功耗分析攻击的椭圆曲线标量乘快速实现算法

针对添加伪点加法在抵抗简单功耗分析攻击的同时效率损失过大的问题,为更好兼顾效率与安全,提出一种新的标量乘快速实现算法——随机伪操作法,通过以单片机为核心运算控制芯片的功耗分析平台进行实测分析验证,随机伪操作法不但能够很好抵抗简单功耗分析攻击,而且相对于添加伪点加法运算效率提高30%-50%。     

ONTOGENIC STUDY OF ISOZYMES IN GIANT TIGER PRAWN,PENAEUS MONODON (CRUSTACEA:DECAPODA)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, Penaeusmonodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH,PGD, and PROT). The zymograms showh little variation in the number of isozymic loci expressed duringlarval development compared with results reported for other penaeid prawns. There were specific ontogenicpatterns at some loci. As larvae developed beyond the embryonic stage, there was tendency for intensityof staining and complexity of banding patterns to increase.     

一种基于决策者满意度的多属性决策方法

提出一种基于决策者满意度的多属性决策方法.分别给出构造定量指标的基于决策者满意度的满意度函数模型和定性指标的集对满意度函数模型.以某保险公司为例,对其分公司培训部培训绩效进行综合评价和结果分析,并讨论了该决策方法的优点.     

Debris flow hazard assessment using set pair analysis models: Take Beichuan county as an example

Assessment of debris flow hazards is important for developing measures to mitigate the loss of life and property and to minimize environmental damage. Two modified uncertainty models, Set Pair Analysis （SPA） and modified Set Pair Analysis （mSPA）, were suggested to assess the regional debris flow hazard. A ease study was conducted in seven towns of the Beichuan county, Sichuan Province, China, to test and compare the application of these two models in debris flow hazard assessment. The results showed that mSPA only can fit for value-variables, but not for non value-variable assessment indexes, Furthermore, as for a given assessment index xi, mSPA only considers two cases, namely, when grade value increases with xi and when grade value decreases with xi. Thus, mSPA can not be used for debris flow hazard assessment but SPA is credible for the assessment because there are no limitations when using SPA model to assess the debris flow hazard. Therefore, in this study SPA is proposed for assessing debris flow hazard.     

Impact of Vibrio parahaemolyticus and white spot syndrome virus (WSSV) co-infection on survival of penaeid shrimp Litopenaeus vannamei

White spot syndrome virus(WSSV) is an important viral pathogen that infects farmed penaeid shrimp, and the threat of Vibrio parahaemolyticus infection to shrimp farming has become increasingly severe. Viral and bacterial cross or superimposed infections may induce higher shrimp mortality. We used a feeding method to infect L itopenaeus vannamei with WSSV and then injected a low dose of V. parahaemolyticus(WSSV+Vp), or we fi rst infected L. vannamei with a low-dose injection of V. parahaemolyticus and then fed the shrimp WSSV to achieve viral infection(Vp+WSSV). The effect of V. parahaemolyticus and WSSV co-infection on survival of L. vannamei was evaluated by comparing cumulative mortality rates between experimental and control groups. We also spread L. vannamei hemolymph on thiosulfate citrate bile salt sucrose agar plates to determine the number of V ibrio, and the WSSV copy number in L. vannamei gills was determined using an absolute quantitative polymerase chain reaction(PCR) method. L v My D88 and Lvakt gene expression levels were detected in gills of L. vannamei by real-time PCR to determine the cause of the different mortality rates. Our results show that(1) the cumulative mortality rate of L. vannamei in the WSSV+Vp group reached 100% on day 10 after WSSV infection, whereas the cumulative mortality rate of L. vannamei in the Vp+WSSV group and the WSSV-alone control group approached 100% on days 11 and 13 of infection;(2) the number of Vibrio in the L. vannamei group infected with V. parahaemolyticus alone declined gradually, whereas the other groups showed signifi cant increases in the numbers of Vibrio( P 0.05);(3) the WSSV copy numbers in the gills of the WSSV+Vp, Vp+WSSV, and the WSSV-alone groups increased from 10 5 to 10 7 /mg tissue 72, 96, and 144 h after infection, respectively. These results suggest that V. parahaemolyticus infection accelerated proliferation of WSSV in L. vannamei and vice versa. The combined accelerated proliferation of both V. parahaemolyticus and WSSV led to massive death of L. vannamei.     

Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification

Chinese shrimp(Fenneropenaeus chinensis)is an economically important aquaculture species in China.However,cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze.In this study,fluorescence in-situ hybridization(FISH) was used to identify the chromosomes of F.chinensis.The 5S ribosomal RNA gene(rDNA)of F. chinensis was isolated,cloned and then used as a hybridization probe.The results show that the 5S rDNA was located on one pair of homologous chromosomes in F.chinensis.In addition,triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method.It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F.chinensis.The successful application of FISH in F.chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps.     

一种抗简单功耗分析攻击的模幂算法

在真实环境下对智能卡RSA算法进行了功耗采集,通过简单功耗分析攻击（SPA）提取出了密钥,证明了传统的模幂运算二元表示（BinaryRepresentation,BR）算法存在明显的密钥信息泄露缺陷。分析发现,信息泄露发生在程序的跳转分支处。针对这一缺陷,提出了一种原子化操作与引入随机数相结合的模幂算法。通过操作的原子化统一了模幂算法的执行路径,消除因跳转分支引起的信息泄露;引入随机数对密钥的汉明重量进行掩盖,有效地防止密钥信息的泄露。在MC8051上的实测验证表明,新算法确实具有抗简单功耗分析的特性。     

Ontogenic study of isozymes in giant tiger prawn,Penaeus monodon (Crustacea: Decapoda)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn,Penaeus monodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH, PGD, and PROT). The zymograms showed little variation in the number of isozymic loci expressed during larval development compared with results reported for other penaeid prawns. There were specific ontogenic patterns at some loci. As larvae developed beyond the embryonic stage, there was a tendency for intensity of staining and complexity of banding patterns to increase.     

Chromosomal localization of 5S rDNA in Chinese shrimp (Fenneropenaeus chinensis): a chromosome-specific marker for chromosome identification

Chinese shrimp (Fenneropenaeus chinensis) is an economically important aquaculture species in China. However, cytogenetic and genomic data is limited in the organism partly because the chromosomes are difficult to isolate and analyze. In this study, fluorescence in-situ hybridization (FISH) was used to identify the chromosomes of F. chinensis. The 5S ribosomal RNA gene (rDNA) of F. chinensis was isolated, cloned and then used as a hybridization probe. The results show that the 5S rDNA was located on one pair of homologous chromosomes in F. chinensis. In addition, triploid shrimp were used to evaluate the feasibility of chromosome identification using FISH and to validate the method. It was confirmed that 5S rDNA can be used as a chromosome-specific probe for chromosome identification in F. chinensis. The successful application of FISH in F. chinensis shows that chromosome-specific probes can be developed and this finding will facilitate further research on the chromosomes of penaeid shrimps.     

Viral Diseases of Olive Flounder in Korean Hatcheries

In order to elucidate the state of diseases, especially viral diseases, and to prevent viral diseases from occurring in olive flounder hatcheries, a range of studies, including epidemiological study, were performed from 1997 to 2003. The location of the hatcheries investigated includes several representative sites in the east （Kangnung, Uljin, Pohang, Yangsan, Ulsan, Pusan）, south （Wando, Changheung, Goheung, Yeosu, Namhae, Tongyeong, Geoje, Jeju） and west （Seosan, Kunsan, Gochang, Yeongkwang, Mokpo, Chindo） costal areas of the Korea Peninsula. A total of 2000 cases have been examined in 7 years, in which mortality caused by viral agents accounts for 22%, or 446 cases. Mortalities associated with viral infection considerably increased from 14% in 1997 to 27% in 2003. A variety of viral diseases were observed, and the occurrences of viral epidermal hyperplasia, viral ascites and viral deformity, viral nervous necrosis, and hirame rhabdoviral disease are 14%, 51%, 25%, and 8% respectively. By investigating the viral infection of broodstock flounder, the infection rate of marine birnavirus （MABV） in hatcheries was identified to be approximately 30%, therefore, it is highly necessary to acquire and keep non-infected broodstock fishes.     

海洋鱼类疾病诊断系统中的多参数的证据不确定性推理机制

在分析、归纳、总结不确定性推理相关问题的基础上,提出基于充分性参数、必要性参数和可信度因子(规则强度)等多参数的证据不确定性推理方法。该方法能够表示具有复杂不确定性加权关系的诊断规则,并且能够根据证据规则的约束条件通过数值计算获取规则的不确定性相关参数;通过在开发的海洋鱼类疾病诊断专家系统中的应用,很好地体现了疾病诊断中存在的不确定性,有效地减少了主观因素对诊断结论准确性的影响。     

针对抗边信道攻击的椭圆曲线标量乘法的简单功耗分析

分析了一种运用平衡点加一倍点操作抗边信道攻击的椭圆曲线标量乘法。方法虽然使点加和倍点的功耗不可区分,但是当标量用二进制表示时,标量乘法的运算功耗仍有边信息泄露。对标量乘算法的传统功耗攻击主要集中在点加运算和倍点运算之间的功耗差异上,文中将攻击点转移到标量乘算法中for语句和if语句之间的功耗差异上,并成功对平衡点加一倍点操作后的标量乘功耗曲线实现了简单功耗攻击。     

广西南宁盆地早第三纪沉积相特征

本文论述了南宁盆地的地质构造背景和第三纪地层、沉积相、古地理演化特征,其中侧重对沉积相剖析认为:南宁盆地在中生代以来形成的NE、NW向两组断裂的“X”型构造背景条件下,受NEE、NNE向各两条断裂控制呈菱形状的第三纪陆相断陷盆地;沉积相以湖相为主,河流相、洪积相次之。前者可分为滨湖、浅湖、半深湖等3个亚相,尤以浅湖相发育,后二者仅在古新世至始新世早、中期发育并分布在湖盆边缘;盆地演化过程中始新世时曾一度上升遭受剥蚀,致使瓦窑村组与上覆地层呈不整合接触,由古新世→始新世末盆地中心由SW→NE迁移,盆地范围增大趋势。还指出南湖组与凤凰山组、古亭组是同期(始新世早、中期)异相产物,盆地内产褐煤、粘土与石英砂等矿产。     

主成分分析法在地质灾害危险性综合评价中的应用

介绍了主成分分析法的分类原理和实施步骤以及多指标体系中指标的选取,针对地质灾害的危险性,建立了评价的多指标体系和分级特征,同时分析了该方法在地质灾害危险性综合评价中的应用方法与过程.并通过对某高速公路沿线地质灾害危险性进行分段综合评价,给出了建设工程可能诱发或加剧的地质灾害和建设工程本身可能遭受的地质灾害危害的程度.证明该方法在地质灾害危险性综合评价中是切实可行的.     

猫岭金矿物质成分及金的赋存状态

猫岭金矿赋存于早元古宙辽河群上亚群盖县组二段绢云千枚岩夹石英岩中。矿石的主要金属矿物有毒砂、磁黄铁矿、银金矿和自然金,毒砂、磁黄铁矿是 Au 的主要载体矿物,矿石有益元素主要有 Ag、Zn、Pb、Cu、Sb,主要有害元素为 As。Au 呈晶隙金、裂隙金和包体金赋存于毒砂、磁黄铁矿等矿物中,因此,适于通过堆浸法利用。