植酸酶对凡纳滨对虾生长性能和体成分的影响

在凡纳滨对虾饲料中添加不同量微生物植酸酶(0,500,1000,2000 U/kg),观察虾的存活率、增重率、饲料系数及虾体和虾壳成分,研究饲料中添加植酸酶对凡纳滨对虾生长性能的影响。结果表明:饲养8周后,饲料中添加500~2000 U/kg植酸酶对凡纳滨对虾增重率、存活率和饲料利用无显著影响(P>0.05);植酸酶添加各组与对照组虾体的水分、粗蛋白、脂肪、灰分、总钙和总磷含量差异不显著;虾壳中粗灰分和钙含量在500和1000 U/kg组显著高于对照组和2000 U/kg组(P<0.05),虾壳磷含量在各饲料组间差异不显著。血清磷浓度在对照组和2000 U/kg组显著高于500 U/kg组(P<0.05),血清钙浓度在组间差异不显著,血清碱性磷酸酶活性以对照组最高,显著高于500和1000 U/kg组。结果说明,在特定试验条件下,饲料中添加植酸酶对凡纳滨对虾幼虾的虾壳和血清成分有显著影响(P<0.05),对幼虾生长性能影响不显著(P>0.05)。     

饲料中添加L-肉碱对牙鲆幼鱼生长、生化组成及血液指标的影响

研究0、300、600、900、1 200、1 500 mg/kg 6个L-肉碱添加水平对牙鲆(Paralichthys olivaceus)幼鱼生长、生化组成和血液指标的影响。结果表明:添加量为1 200 mg/kg的处理组牙鲆幼鱼的增重率和特定生长率显著提高,饲料系数显著降低(P<0.05);随着饲料中L-肉碱含量的增加,肝指数呈现下降趋势,且在添加量为900~1 500 mg/kg时,各组较对照组差异显著(P<0.05);饲料中添加L-肉碱显著降低各实验组脏体比(P<0.05),而对各处理组间存活率和肥满度均无影响(P<0.05);肌肉和肝脏中的脂肪含量与L-肉碱添加量呈负相关,当添加量为1 200~1 500 mg/kg时,较对照组差异显著(P<0.05);肌肉中的蛋白含量与L-肉碱添加量呈正相关,但各组差异不显著(P<0.05);饲料中添加L-肉碱降低血清中的谷草转氨酶、胆固醇、甘油三酯和尿素氮含量,且添加量1 200 mg/kg组各指标含量均显著低于对照组(P<0.05);各处理组的血糖含量较对照组均有所增加,其中1 200 mg/kg组血糖含量较对照组提高41.6%(P<0.05);血清中总蛋白和低密度脂蛋白均随着L-肉碱添加量的增加而呈下降趋势,高密度脂蛋白却得以提升,但差异均不显著(P<0.05)。分析认为,在本实验条件下,饲料中添加L-肉碱可促进牙鲆幼鱼生长,且降脂效果显著。建议L-肉碱最适添加量为1 200 mg/kg。     

斜带石斑鱼幼鱼对羟基蛋氨酸铜的需求

在斜带石斑鱼(Epinephelus coioides)幼鱼基础饲料(铜质量分数2.03 mg/kg)中分别添加6个水平的羟基蛋氨酸螯合铜(hydroxy methionine copper,HMC),制成铜质量分数分别为2.03、4.04、6.20、10.67、13.96 mg/kg的实验饲料,饲喂6周后,探讨HMC对幼鱼生长、非特异性免疫酶活性和组织铜沉积的影响,确定幼鱼饲料中铜的最适需要量。结果表明,饲料中添加HMC,对石斑鱼的增重率、特定生长率(SGR)和饲料系数等生长性能的影响有统计学意义(P0.05),以铜质量分数6.20 mg/kg组增重率和特定生长率最高,饲料系数最低,高于或低于2.03和13.96 mg/kg组,差异有统计学意义(P0.05),与4.04和10.67 mg/kg组差异无统计学意义(P0.05);但对成活率、肝体比影响差异无统计学意义(P0.05)。饲料中添加HMC对全鱼水分和粗蛋白含量影响无统计学意义(P0.05),对全鱼粗灰分和粗脂肪含量影响有统计学意义(P0.05)。石斑鱼血浆铜蓝蛋白(CP)的含量、血清和肝脏总超氧化物歧化酶(T-SOD)及铜锌超氧化物歧化酶(Cu-Zn-SOD)活性均以6.20 mg/kg组最高;饲料中铜的添加对血清谷胱甘肽过氧化物酶(GSH-Px)活性和肝脏硫代巴比妥酸反应物(TBARS)含量影响无统计学意义(P0.05)。与对照组相比,饲料中添加HMC均提高鱼体、肌肉和肝脏中铜含量,差异有统计学意义(P0.05),且以13.96 mg/kg组最高。饲料中添加适量的HMC可提高石斑鱼幼鱼的生长性能,提高抗氧化能力。在本实验条件下,以SGR为判据,通过二次回归方程拟合可得,以HMC为铜源,斜带石斑鱼幼鱼铜需要量为7.09 mg/kg。     

蜕壳促长素对斑节对虾、刀额新对虾蜕壳和生长的作用

在对虾人工配合饲料中添加蜕（脱）壳促长素，喂养斑节对虾（ＰｅｎａｅｕｓｍｏｎｏｄｏｎＦａｂｒｉｃｉｕｓ）和刀额新对虾（ＭｅｔａｐｅｎａｅｕｓｅｎｓｉｓＤｅＨａａｎ），观察其促进蜕壳、生长的作用。试验证明：饲料中添加不同浓度（０．５‰。、１．０‰、１．５‰）的蜕壳促长素，均不同程度地促进对虾的蜕壳和生长。添加浓度为１．５％。效果最为明显。值得在生产中应用。     

不同饲料蛋白水平下L-肉碱对新吉富品系尼罗罗非鱼生长和饲料利用的影响

研究了三个蛋白水平(22%、25%、28%)和五个L-肉碱水平(0、200、400、600、800mg/kg)下尼罗罗非鱼生长和饲料利用率。结果表明,随着饲料中蛋白水平的提高,罗非鱼幼鱼的终体重、增重率(WGR)和特定生长率(SGR)呈上升趋势,而饲料系数(FCR)和蛋白质效率(PER)均随饲料蛋白水平的升高而显著下降(P<0.05);同等蛋白水平下,终体重、WGR和SGR均在L-肉碱添加量为200mg/kg时最高,不添加L-肉碱组最小;在添加400mg/kgL-肉碱时FCR最小,而PER最大,与不添加L-肉碱组比较,差异均显著(P<0.05)。说明饲料中蛋白含量和L-肉碱添加量能显著影响罗非鱼幼鱼的生长和饲料利用,而外源性L-肉碱可明显促进罗非鱼生长,提高饲料转化效率。     

抗菌肽对凡纳滨对虾生长和机体免疫的影响

基础饲料(对照组)中添加10g/kg抗菌肽制剂喂养体重0.10±0.01g、体长2.17±0.11cm的凡纳滨对虾(Penaeus vannamei)30d,进行10d的水浴攻毒(鳗弧菌10~8/mL),探讨饲料中添加抗菌肽对凡纳滨虾生长、成活率、免疫保护率,以及肌肉中溶菌酶活性、超氧化物歧化酶活性、总抗氧化能力的影响。结果表明:饲料中添加抗菌肽可显著提高凡纳滨对虾相对增重率、成活率和对致病菌的免疫保护率(P<0.05),但对饲料系数无显著影响(P>0.05);未受外界微生物刺激时,饲料中添加抗菌肽对凡纳滨对虾总抗氧化能力有促进作用(P<0.05),受外界微生物刺激后,饲料中添加抗菌肽喂养凡纳滨对虾可提高对虾超氧化物歧化酶活性(P<0.05)和总抗氧化能力(P<0.01)。因此饲料中添加抗菌肽喂养凡纳滨对虾可提高其机体非特异性免疫。     

不同形式钴对凡纳滨对虾生长和组织钴含量的影响

为研究氨基酸螯合钴对凡纳滨对虾的营养效果,在凡纳滨对虾配合饲料中添加5、10、15 mg/kg的氯化钴和蛋氨酸钴。饲养8周,4周末(饲养中期)称重,8周末称重并采集对虾肝胰脏和肌肉,测定肝胰脏和肌肉中的钴含量。结果表明:钴的添加形式和钴水平不影响对虾0~4周增重率,15 mg/kg蛋氨酸钴显著提高了对虾0~8周增重率,且两种形式的钴之间差异显著(P<0.05)。51、0 mg/kg的蛋氨酸钴和5、101、5 mg/kg的氯化钴不影响8周末对虾的增重率。特定生长率、饲料系数和蛋白质效率的变化趋势与增重率类似。钴的添加形式和钴水平不影响肌肉中的钴含量,15 mg/kg的氯化钴组肝胰脏中钴含量显著高于对照组(P<0.05),其他各组间无显著差异;蛋氨酸钴各组肝胰脏中钴含量无显著差异;钴的添加形式不影响肝胰脏中钴含量。统计结果表明,促进生长方面,两种钴之间存在显著差异,蛋氨酸钴的营养效果好于氯化钴。饲料中蛋氨酸钴的添加量为15 mg/kg时,对虾生长最快,5 mg/kg时,可满足需要。     

饲料中添加4种中草药制剂对大口黑鲈幼鱼生长和抗氧化能力的影响

【目的】研究4种中草药制剂对大口黑鲈幼鱼生长和抗氧化能力的影响。【方法】在基础饲料中分别添加姜黄素(50 mg/kg)、复方中草药Ⅰ(板蓝根、大黄,5 g/kg)、复方中草药Ⅱ(黄芩、黄柏、大黄、大青叶,5 g/kg)、复方中草药Ⅲ(辣蓼、青蒿、苍耳草、赤小豆、苦杏仁,5 g/kg),连续饲喂初始体质量(19.53±5) g大口黑鲈幼鱼60d,表观饱食投喂,每天投喂2次。【结果】饲料中添加4种中草药制剂对大口黑鲈幼鱼成活率、特定生长率、饲料系数的影响不显著(P0.05);饲料中添加复方中草药Ⅱ显著提高血浆SOD活性(P0.05),复方中草药Ⅲ显著增高肝脏中脂肪含量(P0.05)。【结论】饲料中添加适量的4种中草药制剂对大口黑鲈幼鱼生长不造成不利影响,复方中草药Ⅱ可提高机体抗氧化能力,但复方中草药Ⅲ会加大幼鱼肝脏病变风险。     

复合酸化剂对鲤生长及消化酶活性的影响

在鲤鱼(Cyprinus carpio)基础饲料中,分别添加不同梯度的复合酸化剂(质量分数分别为0、0.2%、0.3%),研究复合酸化剂对其生长和消化酶活性的影响。结果表明:1)添加复合酸化剂显著(P<0.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标;2)添加质量分数0.3%的复合酸化剂时,鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P<0.05),酸化剂组的淀粉酶和脂肪酶活性有升高的趋势,但与对照组差异不显著;3)鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。综上所述,鲤鱼饲料中添加以柠檬酸、乳酸、磷酸等为主的复合酸化剂,可显著提高鲤鱼肝胰脏、前肠和中肠的蛋白酶活性,促进鲤鱼生长。     

2种乳酸菌对军曹鱼幼鱼生长及消化酶、免疫酶活性的影响

【目的】研究配合饲料中添加乳酸杆菌和粪肠球菌对军曹鱼幼鱼生长、消化酶和免疫酶活性的影响。【方法】在基础饲料中分别按1.0×10~8、3.0×10~8、5.0×10~8cfu/g单独喷洒乳酸杆菌和粪肠球菌两种菌,制作6种饲料,投喂初始体质量(10.66±0.09)g的健康军曹鱼幼鱼10周,以基础饲料为对照组,测定幼鱼生长性能、消化酶和免疫酶指标。【结果】与对照组比,乳酸菌组幼鱼饵料系数降低(P0.05),增重率和特定生长率呈现先升后降的变化趋势,并显著高于对照组(P0.05),乳酸杆菌添加组的增重率和特定生长率整体显著高于粪肠球菌添加组(P0.05),在3.0×10~8cfu/g乳酸杆菌添加组达到最大值;乳酸菌组的淀粉酶(AMS)、胰蛋白酶(TRY)和脂肪酶(LPS)活性均显著高于对照组(P0.05);两种乳酸菌添加组的超氧化物歧化酶(SOD)和溶菌酶(LZM)活性高于对照组,而丙二醛(MAD)活性显著低于对照(P0.05)。【结论】以喷洒方式在饲料中添加乳酸杆菌和粪肠球菌,可显著提高军曹鱼的生长性能、消化能力和非特异性免疫力,影响效果与添加量和添加乳酸菌种类有关,乳酸杆菌对军曹鱼的相关作用效果优于粪肠球菌,以3.0×10~8cfu/g乳酸杆菌的效果最佳。     

大豆饼代替部分鱼粉饲养罗非鱼可行性的试验

本试验以大豆饼作为主要蛋白源取代部分优质鱼粉饲养罗非鱼。结果表明,以大豆饼取代50%鱼粉并添加4.8%油脂的饲料是可行的,具有一定的实际意义。而过多地以植物蛋白代替鱼蛋白显然还存在不少问题。     

光合细菌在水产养殖中应用的研究(3)——光合细菌作为仔鱼开口饵料的价值

本文所报导的是用光合细菌作为仔鱼开口饵料的效果。实验结果表明,单纯使用光合细菌,鱼(?)成活率较高,但因营养成份不全面,仔鱼生长速度较慢;如将光合细菌和其它饵料混合使用,则仔鱼在体长、体重和成活率方面均有显著提高。     

军曹鱼仔鱼期的摄食与生长

以人工孵化的军曹鱼(Rachycentron canadum)卵黄囊期仔鱼为材料,对仔鱼早期阶段的摄食及生长情况进行研究。结果表明:在水温28~32℃条件下,军曹鱼仔鱼3日龄开始摄食,6日龄时卵黄完全耗尽,6~7日龄饥饿仔鱼进入不可逆点(PNR)期;混合营养期3~4 d。饥饿仔鱼的初次摄食率开始较低,此后上升,到卵黄囊快要耗尽时达高峰(50%),以后又下降。内营养期,仔鱼日平均生长率为0.86 mm/d;PNR前的摄食期,饥饿仔鱼的日平均增长率为0.068 mm/d,而摄食仔鱼的日平均生长率为0.64 mm/d;PNR后至死亡前,饥饿仔鱼体长呈负增长,而摄食仔鱼的日平均生长率达1.04 mm/d。摄食仔鱼在孵化后8 d内的全长和日龄呈指数关系;前10 d的口径与全长呈正比关系。     

Growth and feed efficiency of juvenile shrimp Litopenaeus vannamei fed formulated diets containing different levels of poultry by-product meal

This feeding trial was conducted to evaluate the potential of poultry by-product meal (PBM) as a protein source in the culture ofLitopenaeus vannamei. Seven isonitrogenous and isoenergetic diets were formulated to near to commercial diet with about 40% protein and 7.5% lipid. Fish meal was replaced by 0, 30%, 40%, 50%, 60%, 70% and 100% of PBM (diets 1-7). The diet with 100% fish meal was used as a control (diet 1). Post-larvae were reared in an indoor semi-closed re-circulating system. Each dietary treatment was tested in 4 replicate tanks (260 L) of 40 shrimp, arranged in a completely randomized design. The shrimps were hand-fed for three times a day to near-satiation (0700, 1200 and 1800) for 60d. Percentage weight gain, survival, feed conversion ratio (FCR), protein efficiency ratio (PER) and body composition of shrimps were measured. There were no significant differences (P>0.05) in growth performance among shrimps fed diets 1-5 (0-60% fish meal replacement). However, shrimps fed diet 7 (100% fish meal replacement) had significantly lower (P<0.05) growth than those fed diets 1-5 (0-60% fish meal replacement). Shrimp fed diets 2-4 (30%-50% fish meal replacement) showed significantly higher growth than those fed diets 6 and 7 (70% and 100% fish meal replacement, respectively). Survival ranged from 94.7% to 100.0% and did not differ significantly (P>0.05) among different experimental diets. No differences in body composition were found among shrimps fed different diets. These results showed that up to 70% of fish meal protein can be replaced by PBM without adversely affecting the growth, survival, FCR, PER and body composition of Litopenaeus vannamei.     

Feasibility of partial replacement of fishmeal with proteins from different sources in diets of Korean rockfish (Sebastes schlegeli)

An 8-week feeding experiment was conducted in an indoor recirculation seawater system to investigate the effects of partial replacement of dietary fishmeal with proteins from five sources on the growth performance and feed utilization of Sebastes schlegeli. Six isonitrogenous and isoenergetic diets were formulated using fishmeal (FM, the control) as sole protein source, or proteins from five sources including poultry by-product meal (PBM), meat and bone meal (MBM), soybean meal (SBM), cottonseed meal (CSM) and canola meal (CNM). Fifteen percent of the crude protein provided by fish meal was replaced, respectively. The results showed that the differences in specific growth rate (SGR) and survival rate (SR) among fish fed PBM, MBM, SBM, CSM and whole FM diets were not significant. However, SGR and SR of fish fed CNM diet was significantly lower than that of other treatments. Feeding rate, feed conversion, nutrient retention showed similar patterns to that of growth. Fish fed CSM and CNM showed significantly lower apparent digestibility coefficient (ADC) of dry matter and gross energy than those fed others while fish fed CNM showed lower ADC of crude protein than those fed others (P<0.05). These results suggested that it was feasible to substitute 15% dietary protein provided by fishmeal with PBM, MBM, SBM and CSM, respectively, but not with CNM as the replacement with CNM reduced fish growth and feed utilization.     

不同饵料强化剂对龙虎斑仔稚鱼生长、存活率、消化酶活力及体成分的影响

褶皱臂尾轮虫(Brachionus plicatilis)与卤虫(Artemia franciscana)无节幼体分别以浓缩小球藻、深海鱼油和鱼肝油强化后,投喂龙虎斑(Epinephelus fuscoguttatus♀×E.lanceolatus♂)仔稚鱼,研究不同强化剂对仔稚鱼生长、存活率、消化酶活力及体成分的影响。结果显示,3种强化剂强化后的生物饵料均可显著提高龙虎斑仔稚鱼的存活率、体长和体质量,差异有统计学意义(P0.05),但对鱼体粗脂肪含量以及肠淀粉酶活力的影响无统计学意义(P0.05)。深海鱼油组仔稚鱼存活率最高,浓缩小球藻可以显著提高胃、肠蛋白酶活性和鱼体粗蛋白含量,各组差异有统计学意义(P0.05),而深海鱼油和鱼肝油可以显著提高肠脂肪酶活性(P0.05)。结果表明,3种强化剂各具优点,均可用于龙虎斑仔稚鱼生物饵料的强化,尤其以深海鱼油提高存活率最显著。     

Effects of florfenicol exposure on growth,development and antioxidant capacity of flounder Paralichthys olivaceus larvae at different developmental stages

Antibiotics are widespread in various environmental media,and may pose a potential threat to aquatic ecosystems and non-target aquatic organisms.Florfenicol(FLO) is one of the most commonly used antibiotics in aquaculture,and extensively used to substitute chloramphenicol with its strong sterilization and low adverse ef fect.In this study,flounder Paralichthys olivaceus,an important economic fish species in seawater was used as an experimental subject.Five exposure concentrations of FLO(including environmentrelated concentrations) were set at 0,0.01,0.1,1,and 10 mg/L.Ef fects of FLO exposure for 168 h on growth and development,motor behavior,antioxidant enzyme activity,malondialdehyde(MDA) content,and thyroid hormone level of P.olivaceus larvae were studied in pre-larvae(1 dpf) and post-larvae(20 dpf).The results show that the short-term FLO exposure could promote the larvae growth to some degrees,but inhibit them as the exposure time prolonged.For pre-larvae,FLO at 0.01 mg/L could stimulate the motor nerve system and increase the swimming ability,but inhibited it at 1 mg/L.With the increasing dosage of FLO,the superoxide dismutase(SOD) and MDA contents were elevated,reaching the maximum in the 1 mg/L FLO group.The pre-larvae were more sensitive than the post-larvae to FLO in the environment,and the growth and immune resistance could be damaged with long exposure.Post-larvae were more tolerant to external pollutants,FLO at 1 mg/L could promote the motor behavior and reduce SOD and MDA contents.Therefore,FLO can be used as an antibiotic at a proper concentration but as a drug to prevent disease in a long-term way.     

A Study on the Contribution of Different Food Sources to Shrimp Growth in an Intensive Fenneropenaeus chinensis Pond

Stable isotope methods can be used to determine the food sources and prey items of aquatic organisms accurately andreliably.This study examined the relative contribution of artificial foods (the formulated feed and Artemia) and natural foods toshrimp growth in an intensive Fenneropenaeus chinensis pond by using carbon and nitrogen stable isotopes.The results showed that the nutrition utilization efficiency of the harvested shrimp was low,only 33.18% of feed nitrogen and 21.73% of feed carbon beingconverted to shrimp flesh.Our stable isotope results showed that the shrimp obtained nutrition for maximum growth from artificialfoods,whose contribution was 93.5%,with the remaining attributed to the natural foods.However,there was 0.94 t harvested shrimpderived from natural foods (the rest of 13.56t harvested shrimp derived from artificial foods) in lha intensive pond with a shrimpproduction of 14.50 tha-1.Therefore,unit area shrimp production can be increased by increasing the contribution proportion of natu-ral foods in intensive shrimp farming.     

盐度渐变和骤变对褐点石斑鱼存活和摄饵的影响

以人工育苗所得的褐点石斑鱼苗为材料,水温28～31.5℃下,盐度范围0～70以组间距5共设置15个梯度,分别进行盐度渐变和骤变对鱼苗存活和摄饵影响的研究。结果表明:渐变实验中,以5/d升高或者降低盐度时,盐度10～55之间,鱼苗存活率100%;而在盐度降低至5时,平均存活时间为15.16 h,24 h存活率19.1%;盐度降至0,平均存活时间7.92 h,24 h存活率为0,盐度0时其平均存活时间与盐度5时的平均存活时间有极显著差异(P<0.01);盐度60,鱼苗基本不摄食,24 h平均存活时间38.51h,24 h存活率为89.8%,24～48 h存活率为89.8%;盐度65,鱼苗不摄食,平均存活时间10.95 h,24 h存活率0%;盐度65平均存活时间与盐度60之间有极显著差异(P<0.01);鱼苗在盐度25～45间摄食活动活跃。骤变实验中,盐度5～50,鱼苗存活时间>48 h;盐度0,24 h存活率5%,48 h存活率为0;盐度55,24 h存活率100%,24～48 h间存活率83.3%;盐度60,24 h存活率44.1%,48 h存活率降至11.1%;鱼苗在盐度65、70中24 h存活率0%;盐度0、65、70的处理组48 h平均存活时间的显著短于其它各组,盐度0～5、55～70为褐点石斑鱼苗骤变的极限生存盐度。     

不同蛋白源饲料对褐点石斑鱼幼鱼血细胞发生的影响

以全鱼粉作为唯一蛋白源(D1),用豆粕替代10%、20%鱼粉(D2、D3),玉米蛋白粉替代10%鱼粉(D4),啤酒酵母替代10%鱼粉(D5),配制5组等氮等能饲料,每种饲料设置3个实验组,进行56 d的养殖实验。通过血液和组织涂(印)片、细胞染色和显微观察,研究人工培育的褐点石斑鱼幼鱼外周血液白细胞的分类组成,头肾、脾脏、体肾和肝脏等4种组织中各类血细胞的发生情况,以及不同蛋白源饲料对褐点石斑鱼血细胞发生的影响。结果表明:褐点石斑鱼外周血液中的白细胞由淋巴细胞(53.30%±4.66%)、血栓细胞(35.69%±3.85%)、嗜中性粒细胞(10.34%±3.14%)、单核细胞(0.28%±0.36%)、浆细胞(0.24%±0.34%)和嗜酸性粒细胞(0.15%±0.27%)组成;组织印迹片中,未成熟的红细胞、淋巴细胞和粒细胞主要在头肾印迹片中出现,未成熟的单核细胞主要在头肾和脾脏印迹片中出现,血栓细胞在肝脏印迹片中数量最多,推断褐点石斑鱼幼鱼主要的造血组织是头肾,其次是脾脏;在4种组织中均观察到浆细胞,在体肾印迹片中观察到嗜碱性粒细胞,在肝脏印迹片中观察到巨噬细胞,在头肾印迹片中还观察到巨大原红细胞。显微观察和数据统计分析的结果都表明,投喂5种蛋白源不同的配合饲料,未对褐点石斑鱼4种组织中血细胞的发生情况造成显著影响。