五株微囊藻毒素降解菌的分离与鉴定

有毒蓝藻产生的蓝藻毒素导致地表水的污染已是全球普遍的现象,最常见的毒素是能导致肝癌的肝毒素微囊藻毒素(MC),迄今为止尚无适用的MC驱除技术,微生物降解MC可能是一条有效的途径．本研究以铜绿微囊藻(Microcystis aeruginosa PCC 7806)所产生的MC为底物,从多个形成微囊藻水华的水体中分离获得能显著降解MC的细菌18株,相对降解能力从18．4％到48．2％．对其中5株MC相对降解能力较强的菌,通过形态特征和生理生化特征分析,鉴定出5株菌分别为:不动细菌属(Acinetobacter)、肠杆菌属(Enterobacter)、微杆菌属(Microbacterium)、芽孢杆菌属(Bacillus)和弗拉特氏菌属(Frateuria),均不同于已报道的属种．     

三种碳源对乌梁素海好氧不产氧光合细菌群落结构的影响

好氧不产氧光合细菌(AAPB)营光合异养,在水体碳循环中起着重要作用.但是,碳源对AAPB群落结构的影响尚不明确.本文基于16S rDNA和光合反应中心合成中的关键基因——pufM基因,运用变性梯度凝胶电泳(DGGE)技术,比较分析了在微生物分离培养中常用的3种碳源(葡萄糖、丙酮酸钠、酵母提取物)对乌梁素海水体总细菌及AAPB群落结构的影响.结果表明,不同碳源诱导7~21 d后,水体总细菌群落丰富度及多样性指数均有不同程度的提升,AAPB群落则呈现先升后降的趋势.3种碳源中,丙酮酸钠添加后对总细菌及AAPB菌群的丰富度和多样性的提升效果最好.测序及系统发育结果表明,乌梁素海水体经诱导后新检测到了一些原环境中未发现的菌属,一些具有特殊碳代谢能力的菌属丰度得到了提升.本研究为充分认识和理解AAPB的生态学作用和意义,及其富集、分离和培养提供了基础依据.     

温度对铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)生长及胞外有机物产生的影响

以巢湖优势种铜绿微囊藻(Microcystis aeruginosa)和鱼腥藻(Anabaena sp.)为研究对象,研究不同温度(35、25和10℃)对这两种藻生长特性和胞外有机物产生的影响.结果表明,温度对铜绿微囊藻和鱼腥藻的藻细胞密度、碱性磷酸酶活性和胞外有机物浓度影响显著.25℃是铜绿微囊藻和鱼腥藻最适宜的生长温度,最高细胞密度分别达到3.12×107cells/ml和2.03×107 cells/ml.不同温度下两种藻的碱性磷酸酶活性特征,证实了高温对鱼腥藻生长的抑制和低温对铜绿微囊藻生长的抑制.胞外有机物释放总量受蓝藻生物量和单位细胞有机物释放速率的影响.铜绿微囊藻的溶解性有机碳和胞外总多糖释放量在25℃最高,最大值分别为49.28和38.46 mg/L;而鱼腥藻在35℃时释放量最高,最大值分别为45.82和40.60 mg/L;10℃条件抑制了两种藻的生长及胞外有机物的释放.鱼腥藻胞外多糖含量在35℃培养条件下最高,而铜绿微囊藻在10℃条件下最高,说明不利的生长条件会促进蓝藻胞外多糖的分泌.三维荧光图谱分析结果表明,铜绿微囊藻和鱼腥藻胞外有机物以类蛋白质和类腐殖酸为主,温度主要影响藻细胞胞外有机物浓度,而对有机物种类组成没有影响.     

一株产碳酸酐酶附生菌对铜绿微囊藻(Microcystis aeruginosa)生长的影响

为研究生长环境中微生物对铜绿微囊藻碳代谢的影响,本文分析太湖典型微囊藻水华样品附生菌中产碳酸酐酶(CA)细菌的比例,结果显示CA菌占11.6%;从微囊藻群体中分离获得了一株高胞外CA附生菌P201,通过ITS基因鉴定,该菌为一株荧光假单胞菌(Pseudomonas fluorescence).并研究了该菌在不同浓度HCO3-条件下对铜绿微囊藻生长的影响,结果表明无论是高HCO3-浓度还是低HCO3-浓度环境中,加入该菌对铜绿微囊藻的生长均有促进作用,说明产CA酶附生菌对铜绿微囊藻的生长有一定的促进作用.     

不同来源有色溶解性有机物光化学/微生物降解过程

城市非点源污染向水生生态系统中输入大量的溶解有机物(DOM),对生态系统健康产生重要影响. 有色可溶性有机物(CDOM)是广泛分布于自然水体中的一类成分和结构复杂、含有多种高活性化学官能团的大分子聚合物,是DOM的重要组分,对水生生态系统健康、能量流动及生物地球化学循环有重要影响. 光化学反应和微生物代谢过程被认为是控制水体CDOM转化、降解和循环的主要影响因素. 然而,对城市化如何影响CDOM组成以及光化学和微生物如何相互作用影响城市水体CDOM动态的理解是不足的. 因此,为评估光化学过程和微生物代谢对不同城市水体CDOM降解与转化的贡献,解析不同城市水体CDOM光化学/微生物降解作用机理,本研究在英国伯明翰选择3类具有典型DOM来源的水体样本,通过实验室9 d受控培养实验,对比分析光化学以及微生物影响下CDOM来源和组成的变化. 结果表明：(1)城市河流由于接受上游污水排放及较短的水力滞留时间,含有丰富的芳香性碳,其CDOM光化学活性明显高于湖泊,光化学降解率为16.60%;(2)城市湖泊CDOM受人类活动影响,自生源类荧光成分富集,生物活性高,在微生物培养过程中CDOM增加了62.16%,而相较于城市湖泊,非城市湖泊由于接受周围景观土壤输入的大量腐殖质类CDOM,光照对其降解转化作用较为明显; (3)光化学过程促进了陆源CDOM中大分子类腐殖质物质降解成为生物活性高的小分子化合物,刺激微生物代谢生成类蛋白质类有机物; 以类蛋白质组分为主导的CDOM在光照过程中被转化为难降解状态,生物活性降低,CDOM微生物代谢过程被抑制. 研究成果为城市水体不同CDOM来源及活性差异特征研究提供了新的思路,有助于城市河流的可持续开放与管理.     

光照强度对水华微囊藻(Microcystis flos-aquae)群体大小增长的影响

大量微囊藻群体的形成和聚集是微囊藻水华形成的重要条件.光照强度是影响微囊藻生长的重要因素之一.为了了解光照强度对水华微囊藻(Microcystis flos-aquae)群体大小增长的影响,以太湖微囊藻水华优势种之一的水华微囊藻作为研究对象,开展了不同光照强度对水华微囊藻群体大小增长的影响研究.共设置5个不同光强处理组,依次为G1:2000 lx;G2:4000 lx;G3:8000 lx;G4:16000 lx;G5:变化光照强度(模拟野外光强).实验期间,G1~G5组大于100细胞群体的平均大小分别为255、480、630、763和662 cells/群体.胞外多糖含量分析显示水华微囊藻形成的群体越大,胞外多糖含量越高.结果表明,低光照强度不利于太湖水华微囊藻群体大小的增长,而变化光照强度和高光照强度有利于水华微囊藻群体大小的增长.研究结果解释了太湖夏季野外变化光照强度和高光照强度有利于微囊藻水华形成的原因.     

微囊藻胶鞘多糖和水溶性胞外多糖的化学特性

微囊藻(Microcystis)产生大量胞外多糖(EPS),包括包裹在细胞外的胶鞘多糖(CPS)和释放到周围环境中的水溶性多糖(RPS).为探究EPS在蓝藻水华发生中的生理生态学意义,迫切需要了解微囊藻EPS的化学特性.本文从太湖分离群体微囊藻,经过大约18个月实验室培养后,其中一些藻株转变为单细胞形态.选择5株群体藻和4株单细胞藻,比较分析这些藻株EPS的化学特性发现:(1)所有9株藻的EPS均为含有脱氧己糖的酸性杂多糖;(2)所有9株藻的CPS的糖醛酸含量(1.2%~2.1%)均低于RPS的糖醛酸含量(2.4%~6.2%);(3)所有9株藻的EPS均含有乙酰基和硫酸基,其中,每一株藻CPS的乙酰基含量均高于RPS的乙酰基含量,所有群体藻CPS的乙酰基含量(4.1%~6.6%)高于所有单细胞藻CPS的乙酰基含量(2.0%~3.2%).本文进而讨论了EPS化学特性对EPS水溶性和微囊藻群体形成的影响,以及对其生态学作用的影响.在这些化学特性中,乙酰取代基团被认为可能是影响微囊藻EPS生理生态学作用的重要因素.     

安徽升金湖越冬白头鹤（Grus monacha）肠道菌群组成的时间变化

肠道微生物对于维持动物消化道内环境的稳定具有重要意义,而肠道微生物菌群结构也受寄主食物成份等外界环境因素的影响.研究动物肠道微生物菌群结构可以更好地了解动物生活状况.以升金湖越冬白头鹤的粪便作为研究对象,以微生物16S rRNA的V3~V4区作为标记基因,进行高通量测序,分析升金湖越冬不同时期白头鹤肠道菌群结构的差异性.研究结果表明:升金湖越冬期不同时期白头鹤粪便样品中微生物多样性指数如香农威纳多样性指数和辛普森指数无显著性差异.但是越冬期不同阶段的粪便样品中菌群组成存在差异,主要包括:在门分类水平,越冬前期拟杆菌门含量较高,厚壁菌门含量较中后期低;越冬中期放线菌门含量较高;越冬后期变形菌门含量较高.在属分类水平,相对于其他两个时期,越冬前期普氏菌属(Prevotella)含量较高,越冬中期杆菌属(Lysinibacillus)含量较高,越冬后期梭菌属(Clostridium)含量较高,并且普氏菌属含量从越冬前期到中期、再到后期有明显的下降趋势,而梭菌属含量从越冬前期到中期、再到后期有明显的升高趋势.这些结果表明不同越冬时期白头鹤肠道菌群组成不同,越冬期不同阶段的食物变化可能作为一个影响因素.     

菌藻共固定化降解微囊藻毒素

研究了微囊藻毒素降解菌S3和椭圆小球藻L1共固定化后,对微囊藻毒素(MC-LR)的降解作用.结果表明,共固定化藻菌体系比单独固定化菌对MC-LR有更好的降解效果,共固定的藻可促进降解菌S3的生长.固定化细胞对毒素的降解能力较稳定,受环境温度和pH变化的影响较小,重复使用仍可维持较高的降解活性.     

细菌群落组成对微囊藻水华分解过程的响应

为探究细菌群落组成对微囊藻水华腐败分解过程的响应,在太湖梅梁湾沿岸进行为期11 d的原位围隔实验,模拟蓝藻水华聚集分解过程,并监测了此过程中水体环境因子和细菌群落组成.结果表明,水体理化因子和细菌群落组成在微囊藻水华分解的过程中发生了显著变化.冗余分析显示细菌的群落组成与水体氧化还原环境(溶解氧或氧化还原电位)、pH值、浮游植物生物量和营养盐浓度(总磷、硝态氮浓度)密切相关.研究还发现了某些与微囊藻水华分解密切相关的特殊细菌类群,其中隶属于黄杆菌科(拟杆菌门)的一个类群在微囊藻厌氧分解的阶段占据显著优势,其功能有待于进一步研究.     

Structure of microbial communities and hydrocarbon-dependent sulfate reduction in the anoxic layer of a polluted microbial mat

The bacterial communities in the anoxic layer of a heavily polluted microbial mat and their growth on hydrocarbons under sulfate-reducing conditions were investigated. Microbial communities were dominated by members of Alphaproteobacteria (27% of the total rRNA), Planctomycetes (21.1%) and sulfate-reducing bacteria (SRB: 17.5%). 16S rRNA cloning revealed sequences beloning to the same bacterial groups with SRB affiliated to the genera Desulfobulbus, Desulfocapsa, Desulfomicrobium, Desulfobacterium and Desulfosarcina/Desulfococcus. The derived enrichment cultures on crude oil, hexadecane and toluene were dominated by SRB. While most SRB sequences of the toluene and hexadecane cultures were related to the sequence of Desulfotignum toluolica, the crude oil enrichment showed a more diverse bacterial community with sequences from the genera Desulfotignum, Desulfobacter, Desulfatibacillus, Desulfosalina, and Desulfococcus. We conclude that the anoxic layer of the studied mats contains a diverse community of anaerobic bacteria, dominated by SRB, some of which are able to grow on hydrocarbons.     

Darstellung der mikrobiellen Besiedlungsstruktur verschiedener Grundwasserhabitate durch Anwendung molekularbiologischer Methoden

Community Structures of Different Groundwater Habitats Investigated Using Methods of Molecular Biology The degradation of pollutants in groundwater and aquifers depends on microbiological and hydrogeochemical processes. To understand the transport and fate of anthropogenic compounds during bank filtration and artificial recharge of groundwater it is necessary to gain more information about the structure of microbial populations in these systems. The population structure of aerobic, anaerobic groundwater habitats and of water samples during artificial groundwater recharge was examined by 16S rDNA based analysis. Water and sediment samples were collected from a groundwater catchment area with artificial groundwater recharge near the river Ruhr in NW-Germany. 16S rRNA genes of mixed bacterial DNA from different samples were amplified by PCR (polymerase chain reaction) with eubacterial primer sequences. To reveal eubacterial population structure amplified PCR-products were separated by DGGE (denaturing gradient gel electrophoresis) on the basis of melting domain structure and nucleotide composition. DGGE patterns of groundwater enrichment cultures and groundwater samples were compared to demonstrate differences between the use of cultivation dependent and molecularbiological approaches. The DGGE pattern of groundwater is very complex and differs significantly from DGGE patterns of groundwater enrichment cultures characterized by a small number of distinct bands. This shows the small quantity of culturable microorganisms in groundwater eco-systems. Aerobic and anaerobic groundwater and sediment samples differ markedly in their DGGE profiles. Different hydrogeochemical zones of this groundwater catchment area are mirrowed by distinct DGGE patterns indicating changes in microbial community structure.Analysis of bacterial population structure in the course of artificial groundwater recharge shows identical DGGE patterns comparing surface water samples to samples taken be-fore gravel prefiltration and before sand filtration. In contrast the DGGE pattern of artificial recharged groundwater differs markedly, indicating significant changes in microbial population during underground passage.     

The effects of adsorptive materials on microbial community composition and PAH degradation at the sediment capewater interface

Capping with layers of inert or adsorptive materials is used to control the release of polycyclic aromatic hydrocarbons(PAH)in sediment but little is known about microbial degradation processes in these materials.A rich native microbial community inhabits the sediment bed,and capping media can influence enrichment and biodegradation activity of benthic microorganisms.The aim of this study was to evaluate the effects of capping media(sand,organoclay,and powdered activated carbon[PAC])on microbial communities under oxic conditions typical of the capewater interface,where naphthalene degradation(model PAH)is likely to be maximized.Bench scale experiments compared naphthalene concentrations,nahAc biomarker abundance,microbial community composition,and cellular attachment in systems amended with adsorptive and non-adsorptive capping materials.Results indicate that activated carbon promoted and enhanced bioactivity;PAC treatments showed high biodegradation rates,nahAc biomarker levels,and attached biological growth consistent with enrichment of the PAHdegrading genus Pseudomonae.In contrast,sand did not enhance biological activity compared to media-free systems.Naphthalene strongly influenced microbial community composition at the species level in all treatments except organoclay,which promoted biological signatures commonly associated with impeding degradation activity.Data overall suggest that adsorptive capping materials can both promote(PAC)and inhibit(organoclay)bioactivity in the surficial layer of caps,indicating that media selection is critical to the design of bioactive capping systems.     

PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary, China

Sixteen sediment samples collected from eight transects in a mangrove swamp of the Jiulong River Estuary, Fujian, China were investigated for their content of polycyclic aromatic hydrocarbons (PAHs) and the biodegradation potential of the indigenous microorganisms. The bacterial community structures in the mangrove sediments and in enrichment cultures were also investigated. The results showed that the total PAHs concentration of mangrove sediments ranged from 280 to 1074 ng g(-1) dry weight, that the PAHs composition pattern in the mangrove sediments was dominated by high molecular weight PAH components (4-6 rings), and that Benzo[ghi]perylene and Indeno[1,2,3-cd]pyrene were the most dominant at different stations. Abundant PAH-degrading bacteria were found in all the stations, the values of phenanthrene-degrading bacteria ranged from 5.85 x 10(4) to 7.80 x 10(5) CFU g(-1) dry weight, fluoranthene-degrading bacteria ranged from 5.25 x 10(4) to 5.79 x 10(5) CFU g(-1) dry weight, pyrene-degrading bacteria ranged from 3.10 x 10(4) to 6.97 x 10(5) CFU g(-1) dry weight and the benzo(a)pyrene-degrading bacteria ranged from 5.25 x 10(4) to 7.26 x 10(5) CFU g(-1) dry weight. DGGE analysis of PCR-amplified 16S rDNA gene fragments confirmed that there was a remarkable shift in the composition of the bacterial community due to the addition of the different model PAH compound phenanthrene (three ring PAH), fluoranthene(four ring PAH), pyrene(four ring PAH) and benzo(a)pyrene(five ring PAH) during enrichment batch culture. Eleven strains were obtained with different morphology and different degradation ability. The presence of common bands for microbial species in the cultures and in the native mangrove sediment DNA indicated that these strains could be potential in situ PAH-degraders.     

Formation of Humic-like Substances in Mixed and Pure Cultures of Aquatic Microorganisms

A natural decay of plant and animal biomass in aquatic environments results in the release of different simple structured organic substances into water. In our laboratory experiments we investigated whether some of them, e.g., starch or peptone, and also a complex yeast biomass can be transformed into humic-like substances by natural assemblages and some pure cultures of aquatic microorganisms. After a 6 months incubation most of the cultural media enriched with those natural organic substrates turned dark in color and humic-like substances (HS) could be isolated. However, the original substrate organic carbon was mainly mineralized in microbial cultures, and only about 3 % C was converted into HS. Total yields of HS differed in dependence of the individual substrate used (peptone > yeast > starch), the origin of inoculum (river > lake > groundwater), and the incubation temperature (20°C > 10°C). According to their elemental composition, and their spectroscopical and electrophoretic characteristics the microbially formed HS resembled natural aquatic humic matter, but were higher in aliphatic constituents (carbohydrates, peptides) and lower in aromatic structures. HS-like substances were also obtained from pure cultures of a bacterium Bacillus sphaericus and a fungus Cladosporium cladosporioides. The ¹³C and ¹⁵N isotopic contents of the microbially produced HS differed in dependence on the microbial inoculum and the type of organic substrates used, but in general they indicated anabolic processes to be mainly responsible for the humification of the simple organic substrates used in our experiments.     

滇池东北部沿岸带生态修复技术研究及工程示范——生态修复目标的确定及其可行性分析

连续6年对星湖仙女湖区水生植被种类、分布、生物量、水体状况进行了调查研究;共采集到水生植物24种,隶属于18科23属,可分为挺水植被、漂浮植被、浮叶植被和沉水植被4种生活型和8个植物群丛,其中苦草群丛占水生植被生物量70％以上,密齿苦草为绝对优势种;2004年与1999年比较,星湖仙女湖水体叶绿素a增加142％,透明度下降54％,水生植被盖度和生物量减少99％,水生植被群落结构趋于简化、多样性下降;星湖仙女湖水生植被发生逆向演替的主要原因是水体污染负荷增加和渔业生产的影响,削减污染负荷和控制渔业生产是促进星湖仙女湖水生植被恢复的重要途径．     

Effect of bioemulsificant exopolysaccharide (EPS2003) on microbial community dynamics during assays of oil spill bioremediation: A microcosm study

Microcosms experiments were carried out to evaluate the effect of bioemulsificant exopolysaccharide (EPS₂₀₀₃) on microbial community dynamics. An experimental seawater microcosm, supplemented with crude oil and EPS₂₀₀₃ (SW + OIL + EPS₂₀₀₃), was monitored for 15 days and compared to control microcosm (only oil-polluted seawater, SW + OIL). Determination of bacterial abundance, heterotrophic cultivable and hydrocarbon-degrading bacteria were carried out during all experimentation period. The microbial community dynamic was monitored by isolation of total RNA, RT-PCR amplification of 16S rRNA, cloning and sequencing. Oil degradation was monitored by GC–MS analysis. Bioemulsificant addition stimulated an increase of the total bacterial abundance, change in the community structure and activity. The bioemulsificant also increased of 5 times the oil biodegradation rate.The data obtained from microcosm experiment indicated that EPS₂₀₀₃ could be used for the dispersion of oil slicks and could stimulate the selection of marine hydrocarbon degraders thus increasing bioremediation process.     

Sustained Ability of a Natural Microbial Community to Remove Nitrate from Groundwater

Microbial-mediated nitrate removal from groundwater is widely recognized as the predominant mechanism for nitrate attenuation in contaminated aquifers and is largely dependent on the presence of a carbon-bearing electron donor. The repeated exposure of a natural microbial community to an electron donor can result in the sustained ability of the community to remove nitrate; this phenomenon has been clearly demonstrated at the laboratory scale. However, in situ demonstrations of this ability are lacking. For this study, ethanol (electron donor) was repeatedly injected into a groundwater well (treatment) for six consecutive weeks to establish the sustained ability of a microbial community to remove nitrate. A second well (control) located upgradient was not injected with ethanol during this time. The treatment well demonstrated strong evidence of sustained ability as evident by ethanol, nitrate, and subsequent sulfate removal up to 21, 64, and 68%, respectively, as compared to the conservative tracer (bromide) upon consecutive exposures. Both wells were then monitored for six additional weeks under natural (no injection) conditions. During the final week, ethanol was injected into both treatment and control wells. The treatment well demonstrated sustained ability as evident by ethanol and nitrate removal up to 20 and 21%, respectively, as compared to bromide, whereas the control did not show strong evidence of nitrate removal (5% removal). Surprisingly, the treatment well did not indicate a sustained and selective enrichment of a microbial community. These results suggested that the predominant mechanism(s) of sustained ability likely exist at the enzymatic- and/or genetic-levels. The results of this study demonstrated the in situ ability of a microbial community to remove nitrate can be sustained in the prolonged absence of an electron donor.     

Microbial water quality of the Georgian coastal zone of the Black Sea

From 2006 to 2008, microbial water quality was monitored along the Georgian coast of the Black Sea. Temperature, pH, salinity, and dissolved oxygen were measured, along with a variety of aquatic microbial parameters, including heterotrophic plate count (HPC), total culturable bacterial count (TCBC), and chlorophyll a (Chl-a) concentration. Total and fecal coliforms and total enterococci counts were recorded as indicators of fecal pollution. Vibrio bacteria, and Escherichia coli- and Vibrio-specific bacteriophages were isolated and enumerated to determine their relationships to standard marine pollution indicators.Persistent microbial pollution was observed, particularly in the summer months, with a higher rate of contamination in estuaries. Microbial indicators generally showed seasonal dependence, suggesting that temperature may influence bacterial dynamics in this environment. No correlation was apparent between fecal pollution indicators and physical-chemical and aquatic microbial parameters, although there were significant relationships amongst the indicators themselves, as well as with the prevalence of Vibrio bacteria and phage.     

丹江口库区表层沉积物细菌多样性及功能预测分析

湖泊沉积物微生物是水生态系统的重要组成部分,目前关于丹江口库区沉积物细菌群落和功能研究鲜见报道.于2017年5月对丹江口库区内5个典型生态点位表层沉积物进行采集,采用高通量测序技术对表层沉积物细菌群落组成进行研究,结果表明细菌群落主要由变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)、疣微菌门(Verrucomicrobia)和硝化螺旋菌门(Nitrospirae)等33个门和318个属组成,具有丰富的群落组成.沉积物细菌群落和理化指标冗余分析(RDA)结果表明,pH值、总磷、有机质和铵态氮含量均能显著影响沉积物细菌群落组成.细菌属分类单元与环境因子Spearman相关分析表明,变形菌门中11个属的细菌与环境因子显著相关,为与环境因子显著相关细菌的主要组成(占47.62%).采用细菌群落功能预测软件PICRUSt(Phylogenetic Investigation of Communities by Reconstruction of Unobserved States)对表层沉积物细菌群落进行分析,结果表明沉积物细菌功能主要涉及能量产生和转换、信号转导机制、氨基酸运输和代谢、细胞壁/细胞膜/膜结构的生物合成等24个基因功能家族,表现出功能上的丰富性.基因功能家族预测基因拷贝数表现为台子山宋岗库心渠首黑鸡嘴.