Effects of ultraviolet radiation on the abundance,diversity and activity of bacterioneuston and bacterioplankton: insights from microcosm studies

The effects of ultraviolet-B (0.4 W m⁻²) radiation on the abundance, diversity and heterotrophic metabolism of bacterioneuston and bacterioplankton communities from Ria de Aveiro (Portugal) were assessed and compared to those of freshwater communities from Lake Vela (Portugal) in microcosm experiments. Exposure to 9 h of artificial ultraviolet radiation (UVR) led to 24–33% reduction in bacterial abundance and up to a 70% decrease in bacterial diversity. Maximum extracellular enzyme activity and monomer incorporation rates were reduced by 16–90% and 80–100%, respectively. Recovery of bacterial activity during post-UV dark incubations ranged from 10 to 100% for extracellular enzyme activity and 40% for monomer incorporation rates. In general, the heterotrophic activity of bacterioneuston was more inhibited by UVR than that of bacterioplankton. However, DGGE profiles revealed greater UVR-induced reductions in the diversity of bacterioplankton compared to bacterioneuston. The similarity between bacterioneuston and bacterioplankton communities in samples collected at early morning was lower than at noon (pre-exposed communities) and increased upon experimental irradiation, possibly indicating selection for UV-resistant bacteria. The observation that UV exposure resulted in enhanced reduction of bacterioneuston activity, but a lower reduction in bacterial diversity accompanied by enhanced dark recovery potential compared to bacterioplankton, indicates re-directioning of bacterioneuston metabolism towards stress defence/recovery strategies rather than the sustained heterotrophic metabolism. Our results indicate that UVR can significantly decrease the abundance, diversity and activity of bacteria inhabiting the surface and sub-surface layers of freshwater and estuarine systems with potentially important impacts on the biogeochemical cycles in these environments.     

Ecosystem water use efficiency in an irrigated cropland in the North China Plain

The eddy covariance technique and the cuvette method were used to investigate water use efficiency in an irrigated winter wheat (Triticum asetivum L.)/summer maize (Zea mays L.) rotation system in the North China Plain. The results show that ecosystem water use efficiency (WUE_e) changed diurnally and seasonally. Daily maximal WUE_e appeared in the morning. WUE_e generally peaked in late April in wheat field and in late July/early August in maize field. From 2003 to 2006, seasonal mean WUE_e was 6.7–7.4 mg CO₂ g⁻¹ H₂O for wheat and 8.4–12.1 mg CO₂ g⁻¹ H₂O for maize. WUE_e was much lower than canopy water use efficiency (WUE_c) under small leaf area index (LAI) but very close to WUE_c under large LAI. With the increase in LAI, WUE_e enlarged rapidly under low LAI but slowly when LAI was higher than one. WUE_e was greater on the cloudy days than on the sunny days. Under the same solar radiation, WUE_e was higher in the morning than in the afternoon. The ratio of internal to ambient CO₂ partial pressure (C_i/C_a) decreased significantly with the increase in photosynthetically active radiation (PAR) when PAR was lower than the critical values (around 500 and 1000 μmol m⁻² s⁻¹ for wheat and maize, respectively). Beyond critical PAR, C_i/C_a was approximately constant at 0.69 for wheat and 0.42 for maize. Therefore, when LAI and solar radiation was large enough, WUE_e has negative correlation with vapor pressure deficit in both of irrigated wheat and maize fields.     

Temporal-spatial variations of euphotic depth of typical lake regions in Lake Taihu and its ecological environmental significance

Euphotic depth can be defined as the portion of wa- ter column that supports the net primary productivity. Its lower end is the critical depth, namely, the depth measured when the daily net primary productivity is zero[1]. In the ecosystems of oceans, lakes and rivers, phytoplankton live in the euphotic depth and euphotic depth is usually taken as the lower boundary, when studying the primary productivity and biomass of phytoplankton; therefore the corresponding depth is sometimes called the t…     

Effect of nutrient supply on photosynthesis and pigmentation to short-term stress (UV radiation) in Gracilaria conferta (Rhodophyta)

The effects of increased photosynthetic active radiation (PAR), UV radiation (UVR), and nutrient supply on photosynthetic activity, pigment content, C:N ratio and biomass yield were studied in tank cultivated Gracilaria conferta (Rhodophyta). Electron transport rate (ETR) and biliprotein content were higher under high nutrient supply (HNS), obtained from fishpond effluents, compared to low nutrient supply (LNS), in contrast to mycosporine-like amino acids (MAAs) dynamic. The high MAA content in LNS-algae could be explained by higher UVR penetration in the thallus and by the competition for the use of nutrients with other processes. Effective quantum yield decreased after short-term exposure to high irradiance whereas full recovery in shade was produced only under slightly heat shock. UVA radiation provoked an additional decrease in photosynthesis under high water temperature. UVB radiation reversed UVA’s negative effect mainly with HNS. Results support that nutrient-sufficiency help G. conferta to resist environmental changes as short-term temperature increase.     

Attenuation of UVR and PAR in a clear and deep lake: Spatial distribution and affecting factors

Light attenuation is considered as a sentinel for environmental change in lakes and has a profound influence on aquatic ecosystems. However, the spatial distribution of ultraviolet radiation (UVR) and photosynthetically active radiation (PAR) attenuation, and the underlying mechanisms are still not fully understood. We carried out a field investigation with 60 sampling sites covering the entire Lake Qiandaohu from November 29 to December 1, 2013, during the weak stratification period to elucidate the spatial pattern and driving mechanisms. The diffuse attenuation coefficient of UVB (K_d(313)), UVA (K_d(340)) and PAR (K_d(PAR)) varied from 1.48 to 4.63 m⁻¹, 1.09 to 3.43 m⁻¹, and 0.26 to 0.94 m⁻¹, respectively. The corresponding ranges for the 1% attenuation depths were from 0.10 to 3.11 m, 1.34–4.21 m and 4.87–17.58 m, respectively. Total suspended matter (TSM) concentration was highly significantly correlated with K_d(313), K_d(340) and K_d(PAR) indicating that TSM was the main driver of UVR and PAR attenuation in Lake Qiandaohu in the late autumn and early winter. TSM concentration, K_d(313), K_d(340) and K_d(PAR) had obvious horizontal spatial heterogeneity presenting a decreasing trend from the estuary area to the center area in the lake. These results suggested that the spatial distribution of TSM from the inflow drived the spatial distribution of UVR and PAR attenuation. Significantly positive correlations were also observed between the chromophoric dissolved organic matter (CDOM) absorption coefficient and K_d(313). TSM and CDOM absorption spectra showed that in the UVR waveband (350–400 nm), the mean relative contribution rates of CDOM (a_g(λ)), non-algal particles (a_nap(λ)), phytoplankton (a_ph(λ)) and pure water (a_w(λ)) to the total absorption were 67.5 %, 24.0 %, 5.0 % and 3.5 %, respectively. In the PAR waveband, the mean relative contribution rates of a_g(λ), a_nap(λ), a_ph(λ) and a_w(λ) to the total absorption were 25.4 %, 18.6 %, 9.4 % and 46.6 %, respectively. Our findings could provide support for ecological environment protection in Lake Qiandaohu considering the importance of UVR and PAR attenuation in aquatic ecosystems.     

Photosynthetically active radiation retrieval based on HJ-1A/B satellite data

Photosynthetically active radiation (PAR) is essential for plant photosynthesis and carbon cycle, and is also important for meteorological and environmental monitoring. To advance China’s disaster and environmental monitoring capabilities, the HJ-1A/B satellites have been placed in Earth orbit. One of their environmental monitoring objectives is the study of PAR. We simulated direct solar, scattered and environment radiation between 400 and 700 nm under different atmospheric parameters (solar zenith angle, atmospheric water vapor, atmospheric ozone, aerosol optical thickness, surface elevation and surface albedo), and then established a look-up table between these input parameters and PAR. Based on the look-up table, we used HJ-1A/B aerosol and surface albedo outputs to derive the corresponding PAR. Validation of inversed instantaneous and observed PAR values using HJ-1 Heihe experimental data had a root mean square error of 25.2 W m^?2, with a relative error of 5.9%. The root mean square error for accumulated daily PAR and observed values was 0.49 MJ m^?2, with a relative error of 3.5%. Our approach improved significantly the computational efficiency, compared with using directly radiation transfer equations. We also studied the sensitivity of various input parameters to photosynthetically active radiation, and found that solar zenith angle and atmospheric aerosols were sensitive PAR parameters. Surface albedo had some effect on PAR, but water vapor and ozone had minimal impact on PAR.     

Photosynthetically active radiation retrieval based on HJ-1A/B satellite data

Photosynthetically active radiation (PAR) is essential for plant photosynthesis and carbon cycle, and is also important for meteorological and environmental monitoring. To advance China’s disaster and environmental monitoring capabilities, the HJ-1A/B satellites have been placed in Earth orbit. One of their environmental monitoring objectives is the study of PAR. We simulated direct solar, scattered and environment radiation between 400 and 700 nm under different atmospheric parameters (solar zenith angle, atmospheric water vapor, atmospheric ozone, aerosol optical thickness, surface elevation and surface albedo), and then established a look-up table between these input parameters and PAR. Based on the look-up table, we used HJ-1A/B aerosol and surface albedo outputs to derive the corresponding PAR. Validation of inversed instantaneous and observed PAR values using HJ-1 Heihe experimental data had a root mean square error of 25.2 W m⁻², with a relative error of 5.9%. The root mean square error for accumulated daily PAR and observed values was 0.49 MJ m⁻², with a relative error of 3.5%. Our approach improved significantly the computational efficiency, compared with using directly radiation transfer equations. We also studied the sensitivity of various input parameters to photosynthetically active radiation, and found that solar zenith angle and atmospheric aerosols were sensitive PAR parameters. Surface albedo had some effect on PAR, but water vapor and ozone had minimal impact on PAR.

     

太湖水面太阳辐射中的光合有效成份

本文利用睛空大气辐射传输分光参数化模式,讨论了到达太湖水面太阳总辐射中光合有效成分所占比例的日变化状况。辐射传输计算所涉及的气象参数采用无锡地区气象站资料;水体反射仅考虑有风浪状况下的水平Fresnel反射。两次湖面实测太阳总辐射日变化曲线与计算值对比分析表明,计算结果基本上能反映晴空条件下太湖水面辐射状况。本文结果对利用总辐射值估算水体生态研究中感兴趣的光合有效成份有一定参考价值。     

Arbuscular mycorrhizal fungi on developing islands within a dynamic river floodplain: an investigation across successional gradients and soil depth

Most vascular plants form symbioses with mycorrhizal fungi that associate with roots and provide nutrients to hosts in exchange for carbohydrates, as well as serve a range of other functions. Mycorrhizal fungi have been studied extensively in upland ecosystems, but we know less about their ecology at aquatic-terrestrial interfaces, especially their distributions at depth. Our objectives were to determine whether abundances of arbuscular mycorrhizal fungi (AMF) change as floodplain islands develop and to describe vertical distributions of AMF from the forest floor to the water table along the freely flowing Tagliamento River in northeastern Italy. We sampled surface sediments (0–10 cm) from three stages of island development on the floodplain—fresh deposits, pioneer islands, and established islands. We also sampled sediments vertically (0–150 cm) from the ground surface to the water table on an established island. We characterized abundance of AMF propagules (colonized roots, spores, and hyphae) within sediments. Roots available to host fungi were absent on fresh deposits; however, some viable spores and hyphae were available at these sites. Pioneer and established islands each had similar hyphal lengths (~860 cm cm⁻³) and colonized root lengths (~3 cm cm⁻³). Abundance of spores increased from depositional (3.5 ± 0.9 (±SE) cm⁻³) to pioneer (17 ± 6.1 cm⁻³) to established (32 ± 6.3 cm⁻³) islands. On an established island, AMF propagules were present at all depths sampled, including at the water table, providing first documentation of these symbionts to such depths in a riparian setting. Mycorrhizal fungi likely link aquatic and terrestrial habitats by connecting plants, soil, and ground water and may influence nutrient transfers among these subsystems.     

Effects of pollen leaching and microbial degradation on organic carbon and nutrient availability in lake water

Nutrient fluxes across terrestrial-aquatic boundaries and their subsequent integration into lake nutrient cycles are currently a major topic of aquatic research. Although pollen represents a good substrate for microorganisms, it has been neglected as a terrestrial source of organic matter in lakes. In laboratory experiments, we incubated pollen grains of Pinus sylvestris in water of lakes with different trophy and pH to estimate effects of pollen input and its subsequent microbial degradation on nutrient dynamics. In this ex situ experiment, we measured concentrations of organic carbon, phosphorus and nitrogen in the surrounding water as well as microbial dynamics (bacteria and fungal sporangia) at well-controlled conditions. Besides leaching, chemical and microbial decomposition of pollen was strongest within the first week of incubation. This led to a marked increase of soluble reactive phosphorus and total dissolved nitrogen (up to 0.04 and 1.5 mg L⁻¹, respectively, after 5 days of incubation) in the ambient water. In parallel, pollen grains were rapidly colonized by heterotrophic bacteria and aquatic fungi. Leaching and microbial degradation of pollen accounted for ≥80, ≥40, ≥50% for organic C, N and P, respectively, and did not significantly differ among water samples from the studied lakes. Thus, pollen introduces high amounts of bio-available terrestrial organic matter and nutrients into surface waters within a short time. A rough calculation on P input into oligotrophic Lake Stechlin indicates that pollen plays an important ecological role in nutrient cycling of temperate lakes. This requires further attention in aquatic ecology.     

The effects of UVR irradiance and spectral composition on yellow perch (<Emphasis Type="Italic">Perca flavescens</Emphasis>) larvae survival

The purpose of this study was to determine experimentally the effects of the quality (UV-A/UV-B ratio) and quantity (irradiance) of natural ultraviolet radiation (UVR) on the survival of yellow perch (Perca flavescens) larvae and on the oxidative stress in their cytoplasm, estimated by the activity of the superoxide dismutase (SOD). We also estimated the potential accumulation of photodamage in DNA by using UV dosimeters. Freshly-hatched yellow perch and UV dosimeters were incubated in controlled conditions under a factorial combination of selective and non-selective filters offering different levels of UVR protection and exposed to natural solar light. Larval survival was inversely related to the UVR intensity gradient, and responded similarly to the presence of both UV-A and UV-B or UV-A only. In contrast, the responses of SOD activity and UV dosimeter were stronger in the presence of both UV-A and UV-B than UV-A only, leading to a partial mismatch with the results on survival. Our results, obtained under natural solar light, suggest that incident UV-A radiation, despite its lower energy per unit photon than UV-B, could be a serious threat for yellow perch larvae. They also show that UV dosimeters and SOD activity predict only some components of UVR risk for yellow perch larvae. The partial mismatch between UV dosimeters results and larval survival cautions against the use of UV dosimeters alone as a proxy for UVR risk in the field.     

Reconstructing the largest explosive eruptions of Mt. Ruapehu,New Zealand: lithostratigraphic tools to understand subplinian–plinian eruptions at andesitic volcanoes

We analysed the tephra record of Mt. Ruapehu for the period 27,097 ± 957 to ~10,000 cal. years BP to determine the largest-scale explosive eruptions expected from the most active New Zealand andesitic volcano. From the lithostratigraphic analysis, a systematic change in the explosive behaviour is identified from older deposits suggesting dry magmatic eruptions and steady eruptive columns, characterised by frothy to expanded pumice fabrics, to younger deposits that are products of unsteady conditions and collapsing columns, characterised by microvesicular, fibrous, and colour-banded pumice fabrics. The end-members were separated by eruptions with steady columns linked to water–magma interaction and highly unstable conduit walls. Dry magmatic eruptions producing steady plinian columns were most common between 27,097 ± 957 and shortly after 13,635 + 165 cal. years BP. Following this time, activity continued with eruptions that produced dominantly oscillating unsteady columns, which engendered pyroclastic density currents, until ~10 ka when there was an abrupt transition at Mt. Ruapehu since which eruptions have been an order of magnitude lower in intensity and volume. These data demonstrate long-period transitions in eruption behaviour at an andesitic stratovolcano, which is critical to understand if realistic time-variable hazard forecasts are to be developed.     

Spectral attenuation of solar radiation in Patagonian fjord and coastal waters and implications for algal photobiology

The spectral attenuation of solar irradiation was measured during summer in two types of coastal waters in southern Chile, a north Patagonian fjord (Seno Reloncaví) and open coast (Valdivia). In order to relate the light availability with the light requirements of upper subtidal seaweeds, the saturating irradiance for photosynthesis (E_k) from P–I curves was measured. In addition the UV risk was assessed. Based on the z_1% of PAR, the lower limit of the euphotic zone in the studied systems averaged 21 m (K_d 0.24 m^?1) in Seno Reloncaví and 18 m (K_d 0.27 m^?1) in the coast of Valdivia. Photosynthesis of the studied seaweeds was saturated at markedly lower irradiances than found in their natural depths at the time of the study. Solar radiation penetrating into these depths at both locations largely supports the light requirements for the photosynthesis of subtidal species: 50–160 μmol m^?2 s^?1 for seaweeds from Seno Reloncaví (7 m tidal range) and 20–115 μmol m^?2 s^?1 for Valdivia assemblages (2 m tidal range). Optimal light conditions to saturate photosynthesis (E_k) were present at 10–16 m water depth. The attenuation of solar irradiation did not vary significantly between the fjord and coastal sites of this study. However, the underwater light climates to which seaweeds are exposed in these sites vary significantly because of the stronger influence of tidal range affecting the fjord system as compared with the open coastal site. The patterns of UV-B penetration in these water bodies suggest that seaweeds living in upper littoral zones such as the intertidal and shallow subtidal (<3 m) may be at risk.     

Optical properties and light penetration in a deep,naturally acidic,iron rich lake: Lago Caviahue (Patagonia,Argentina)

The optical properties and light climate in the deep and extremely acid Lake Caviahue have been studied in order to better understand its characteristics and the possible influence upon the phytoplankton community. The absorption coefficients for the dissolved fraction were maximal in the ultraviolet (UV) region and the water absorption spectra showed a shoulder around 300 nm, which was attributed to the concentration of Fe(III). No radiation was detected in the water column below 360 nm. The depth of the 1% incident radiation was dependent of wavelength, showing its maximum of 13.3 m at 565 nm, compared to 1.7 m and 4.8 m at 400 nm and 700 nm, respectively. Phytoplankton biomass was low and showed an almost constant profile with depth despite the relative darkness of the water column. Optical climate of Lake Caviahue is not typical of high elevation lakes but is more similar to low elevation shallow lakes of the Andean region. The chemical composition of the water, mainly Fe oxidation state and concentration, is the responsible for the high attenuation of the UV radiation (UVR). Living organisms are protected of UVR because Lake Caviahue waters are a shield against UV-B.