Comparative tolerance of two estuarine annelids to fluoranthene under normoxic and moderately hypoxic conditions

The tolerance of the oligochaete Monopylephorus rubroniveus and the polychaete Streblospio benedicti to sediment-associated fluoranthene was characterized under normoxic (>80% dissolved oxygen saturation) and moderately hypoxic ( approximately 50% dissolved oxygen saturation) conditions. Under both conditions, M. rubroniveus was highly tolerant of fluoranthene. Streblospio benedicti was considerably less tolerant of fluoranthene compared with M. rubroniveus. In addition, S. benedicti was less tolerant to fluoranthene under moderately hypoxic conditions, although no differences in sensitivity between the two oxygen conditions were observed based upon median lethal tissue residues. Bioaccumulation factors were higher for S. benedicti exposed to moderate hypoxia, suggesting that behavioral adaptations to compensate for the lower dissolved oxygen increased its bioaccumulation of fluoranthene. The results of the present laboratory study demonstrate that (1). changes in annelid tolerance to fluoranthene under varying oxygen conditions is a species-dependent phenomenon and (2). the differential tolerance of these two annelids to these combined stressors is consistent with their relative abundances in the field.     

Effects of sediment organic matter quality on bioaccumulation, degradation, and distribution of pyrene in two macrofaunal species and their surrounding sediment

Sediment dwelling macrofauna (infauna) are important vectors for the transfer of sediment-associated contaminants to higher trophic levels. Sedimenting organic matter constitutes an important food source for all benthic organisms and changes seasonally in terms of quantity and quality. Sediment organic matter (SOM) quality affects organism activity and feeding behaviour, and is therefore also likely to affect contaminant fate in benthic systems. We investigated the impact of SOM quality (enrichment with either labile Tetraselmis sp. or refractory lignin) on the accumulation and metabolism of sediment-associated pyrene in Nereis diversicolor (Annelida) and Amphiura filiformis (Echinodermata), as well as the combined effect of SOM quality and infaunal bioturbation on pyrene distribution and metabolism in the sediment. After 45 d of exposure, SOM quality almost doubled pyrene bioaccumulation in both species, while pyrene metabolism remained unaffected. Metabolites comprised approximately 80% of the total tissue pyrene in N. diversicolor and approximately 40% in A. filiformis. A. filiformis arms contained one fifth of the disk pyrene concentration. Approximately 20% of the pyrene found in A. filiformis arms was found to be covalently bound to, e.g. DNA, RNA or proteins, thus reducing pyrene bioavailability to arm-cropping predators. The sedimentary pyrene distribution and metabolism was species-dependent, but correlated poorly with prevailing knowledge on species-specific bioturbation patterns. This was attributed to the comparably high biodegradability of the contaminant thus altering its sorptive characteristics and function as inert tracer. Subduction of pyrene and metabolites occurred, and the fraction of pyrene covalently bound to SOM increased with depth, thereby removing pyrene from the bioavailable pool. Our results imply that bioaccumulation and trophic transfer of sediment-associated PAH should increase following fresh organic matter input, e.g. after sedimentation of phytoplankton blooms. We stress the importance of considering behavioural characteristics of infauna and the trophic situation of the system when assessing fate and effects of sediment-associated contaminants.     

Exposure of grass shrimp to sediments receiving highway runoff: Effects on reproduction and DNA

A grass shrimp bioassay was carried out on sediments from three estuarine stations which were different distances from a highway storm drain. Total polycyclic aromatic hydrocarbon (PAH) concentrations were 29, 1.5 and 0.1 μg/g sediment at stations A (next to drain), B (100 m from drain) and C (500 m from drain), respectively. Lower embryo production and embryo hatching rates and a higher level of DNA strand breaks (comet assay) were observed in grass shrimp exposed to stations A and B sediments. There appeared to be an association between reproduction abnormalities and increased DNA strand breaks as a result of grass shrimp exposure to estuarine sediments receiving highway runoff.     

Annelid assemblages in soft bottoms subjected to human impact in the Urías estuary (Sinaloa,Mexico)

The annelid fauna inhabiting soft bottoms was analysed during February and April, 1997 in the Urías estuary which is mostly surrounded by mangrove swamps, but is also subjected to the effect of several anthropogenic activities. Classification and ordination techniques were used to perform the characterisation of the system based on the annelid assemblages according to environmental variables (mainly organic matter content and percentage of mud). Three zones were found: (1) ‘very polluted zone’, in front of a slaughterhouse and a fish processing factory with a total lack of macrofauna due to the strong enrichment of the sediment in organic matter; (2) ‘polluted zones’, characterised by the sporadic presence of oligochaetes in front of a domestic sewage effluent, by the dominance of Capitella capitata close to a thermoelectric plant, and by the dominance of Streblospio benedicti and oligochaetes in the surroundings of an upstream shrimp farm; (3) ‘unpolluted zones’, in the mouth of the estuary and an area surrounded by mangrove trees, with low organic matter content in sediment, high diversity and no dominant species.     

Altered feeding habits and strategies of a benthic forage fish (Fundulus heteroclitus) in chronically polluted tidal salt marshes

Responses in feeding ecology of a benthic forage fish, mummichogs (Fundulus heteroclitus), to altered prey resources were investigated in chronically polluted salt marshes (the Arthur Kill-AK, New York, USA). The diet niche breadth of the AK populations of mummichogs was significantly lower than that of the reference population, reflecting reduced benthic macroinfaunal species diversity. Most of the AK populations also had 2-3 times less food in their gut than the reference population. This disparity in gut fullness among the populations appeared to be partly due to ingested prey size shifts; some of the AK populations ingested fewer large prey than the reference population. Furthermore, benthic assemblages were strongly associated with sediment-associated mercury; gut fullness of the AK populations also significantly decreased with increasing mercury body burdens. These results indicate that chronic pollution may have directly (chemical bioaccumulation) and indirectly (reduced prey availability) altered the feeding ecology of mummichogs.     

Impact of sediment organic matter quality on the fate and effects of fluoranthene in the infaunal brittle star Amphiura filiformis

Hydrophobic contaminants, such as polycyclic aromatic hydrocarbons (PAHs) readily adsorb to organic matter. The aim of this study was to determine the importance of the quality of sedimentary organic matter for the uptake, biotransformation and toxicity of the PAH, fluoranthene (Flu), in the infaunal brittle star Amphiura filiformis. Brittle stars were exposed to a base sediment covered by a 2 cm Flu-spiked top layer (30 microg Flu/g dry wt. sed.), enriched to the same total organic carbon content with either refractory or labile organic matter. The labile carbon source was concentrated green flagellate: Tetraselmis spp. The refractory carbon source was lignin from a paper mill. Tissue concentrations of Flu both in disk and arm-fractions were determined as total Flu, parent Flu (i.e. untransformed), aqueous Flu-metabolites, polar Flu-metabolites and tissue residue Flu (i.e. unextractable). Our results showed that sediment particle ingestion is a pathway by which Flu can enter benthic food webs. Flu toxicity (measured as arm-regeneration), but not net accumulation, was dependent on the nutritional quality of the ingested sediment particles. Flu bioaccumulation could not be attributed solely to equilibrium partitioning between organism lipid content and organic content of the sediment. Biotransformation of Flu by brittle stars was very limited and unaffected by organic matter quality. A. filiformis contributed to the downward transport of Flu from the surface sediment to the burrow lining. The limited breakdown of parent Flu by brittle stars and/or microorganisms was relatively higher in burrows compared to surface sediment, and highest in the presence of labile organic matter. Tissue concentrations were higher in disk than in arms, but the proportion of metabolic products relative to parent Flu was higher in arms than in the disk fraction. We estimate that the yearly mobilization of sediment-associated Flu by arm-regeneration in A. filiformis is in the range of 3.8-29.4 microg total Flu eq. m(-2) year(-1) at a sediment concentration of 30 microg Flu/g dry wt. sed.     

Stress protein expression in Ampelisca abdita (Amphipoda) exposed to sediments from San Francisco Bay

Stress proteins (heat shock proteins, hsps) form part of the cellular protein repair system, and are induced by a wide variety of Stressors. To determine their suitability as tools for assessing sublethal sediment toxicity, we measured levels of members of the stress protein families hsp60 and hsp70 in benthic estuarine amphipods (Ampelisca abdita) exposed to sediments from 23 different sampling sites in San Francisco Bay for 10 d. Concentrations of sediment-associated xenobiotics were determined. Per cent survival was recorded and surviving animals were analysed for stress proteins using western blotting techniques. An inverse correlation (r² = 0.44) was seen between amphipod survival and hsp64 levels, and hsp64 levels were positively correlated with concentrations of total polycyclic aromatic hydrocarbons (PAHs) (r² = 0.5). Principal component analysis revealed that amphipod mortality was linked to a combination of several PAHs (phenanthrene, fluoranthene, pyrene, benzo(a)pyrene) and di-n-butylphthalate at southern San Francisco Bay sites. At northern San Francisco Bay sites, negative correlations were found between hsp64 levels and organotin compounds (MBT, DBT, TBT), and between hsp71 levels and the PAHs, benzo (b,k)fluoranthene and benzo(G,H,I)perylene, suggesting an inhibitory effect of these compounds on stress protein expression.     

Comparative toxicity of drilling muds: role of chromium and petroleum hydrocarbons

Samples of used drilling muds collected during the course of a single well drilling operation exhibited different degrees of acute toxicity to sheepshead minnows and grass shrimp. For moulting grass shrimp, Palaemonetes pugio, the 96-h LC₅₀'s were 360 to 14 560 ppm (μl/litre); many of these values were considerably lower than those reported from previous drilling mud assays. However, when some of the muds used in this study were tested on sheepshead minnows, Cyprinodon variegatus, the resulting 96-h LC₅₀'s (6300 to 100 000ppm) were well within the range of previously reported values.Although a number of the drilling mud samples had relatively high amounts of chromium due to the addition of sodium chromate, there was a low correlation between chromium concentration and toxicity. In only three drilling muds could chromium content alone account for the observed toxicities. Furthermore, chemical analyses revealed the presence of No. 2 fuel oil like petroleum hydrocarbons in the mud samples. Based on the results of toxicity tests with No. 2 fuel oil and the concentrations of oil present in the muds, the toxicity of the mud samples to grass shrimp appears to be largely attributable to the petroleum hydrocarbon content.     

Cadmium toxicity to three species of estuarine invertebrates

Three species of estuarine invertebrates, Palaemonetes pugio (grass shrimp), Pagurus longicarpus (hermit crab) and Argopecten irradians (bay scallop), were exposed to Cd in flowing seawater at concentrations of 0·06, 0·12, 0·25, 0·5 and 1·0 mg/litre. Incipient LC₅₀ values of 0·53 and 0·07 mg/litre were estimated for bay scallop and hermit crab, respectively. The toxicity curve for grass shrimp had not stabilised, but the incipient LC₅₀ value was estimated to fall within a range of 0·2 to 0·3 mg/litre. Short-term response, as measured by time to 50% mortality at the highest Cd concentration, was 10, 21 and 23 days for the bay scallop, hermit crab and grass shrimp, respectively. Scallop growth was inhibited at all exposure concentrations with a measured 42-day EC₅₀ value of 0·078 mg/litre Cd. Byssal thread detachment precedes death in bay scallops. An EC₅₀ value of 0·54 mg/litre Cd for byssal detachment was measured on day 8 of the bioassay before appreciable mortality. This compared favourably with the incipient LC₅₀ value of 0·53 mg/litre Cd. Cadmium accumulation occurred at all concentrations in bay scallop and grass shrimp.     

利用老化虾塘进行斑节对虾与锯缘青蟹混养试验

在老化虾塘中进行斑节对虾(Penaeus monadom Fabricus)与锯缘青蟹(Scyll serrata)混养试验，结果如下：斑节对虾的产量为1225kg／hm2，养成存活率为65．3％：锯缘青蟹的产量为422．5kg／hm2，存活率为40．2％；纯收入42105元／hm2，投入产出比达到1：2，取得了较好的经济效益。     

Bioenhancement of cadmium transfer along a multi-level food chain

Previous studies have shown that metal partitioned to a subcellular compartment containing trophically available metal (TAM) is readily available to predators and may be enhanced by increased binding of metal to heat-stable proteins (HSP - e.g., metallothioneins). The aim of the current investigation was to determine the influence of TAM on the trophic transfer of Cd along an experimental, three-level food chain: Artemia franciscana (brine shrimp)-->Palaemonetes pugio (grass shrimp)-->Fundulus heteroclitus (mummichog). P. pugio were fed for 7 days on A. franciscana exposed to Cd in solution (including (109)Cd as radiotracer) and subjected to subcellular fractionation or fed to F. heteroclitus. An HSP-driven increase in the percentage of Cd associated with TAM (TAM-Cd%) in A. franciscana exposed to 1 muM Cd resulted in a bioenhancement (i.e., a greater than linear increase with respect to A. franciscana exposure) of Cd trophic transfer to P. pugio. Increased dietary Cd exposure did not affect TAM-Cd% in P. pugio nor trophic transfer to F. heteroclitus.     

A laboratory and field comparison of sediment polycyclic aromatic hydrocarbon bioaccumulation by the cosmopolitan estuarine polychaete Streblospio benedicti (Webster)

Polycyclic aromatic hydrocarbon (PAH) bioaccumulation by the polychaete worm Streblospio benedicti (Webster) was measured under exposure to PAH-contaminated sediments in the field and for 28 d in the laboratory. Streblospio benedicti collected from field sediments contaminated at 2.94, 1.07, and l.52 μg g⁻¹ fluoranthene (FLU), benz[a]anthracene (BAA), and benzo[a]pyrene (BAP), bioaccumulated those PAHs at 1.53, 0.215, and 0.332 μg g⁻¹, while worms isolated from less contaminated field sediments (0.399, 0.228, 0.288 μg g⁻¹ FLU, BAA, and BAP) had FLU, BAA, and BAP body burdens of 0.543, 0.236, and 0.083 μg g⁻¹. Worms incubated for 28 d in PAH-spiked sediments (1.52, 0.991, 0.504 μg g⁻¹ FLU, BAA, and BAP) bioaccumulated those PAHs at 0.382, 0.966, and 0.602 μg g⁻¹, respectively. Data normalization to organism lipid and sediment organic carbon (biota-sediment accumulation factors [BSAFs] strongly suggest that Streblospio PAH bioaccumulation was directly related to percent sediment organic carbon, but BSAFs were substantially lower than predicted by equilibrium partitioning theory. BSAFs decreased with increasing PAH log K_ow, in worms collected from field sediments, but in spiked sediments BSAFs increased with increasing PAH hydrophobicity. This disparity may have been caused by insufficient spiked-sediment equilibration time (1.5h) in the case of the laboratory test sediments.     

Behavior and brain chemistry correlates in mummichogs (Fundulus heteroclitus) from polluted and unpolluted environments

The ability of Fundulus from a polluted environment to capture a prey organism, the grass shrimp (Palaemonetes pugio), was compared to that of Fundulus from an unpolluted reference site. Fish from the unpolluted site took significantly more prey (p < 0.1) during the first 5–10 min of each trial than fish from the polluted site.Analysis of the neurotransmitters dopamine (DA) and serotonin (5-HT) and their metabolites, DOPAC, homovanillic acid (HVA), and 5-hydroxy-indolacetic acid (5-HIAA) in cerebellums and medullas was performed by HPLC. Mummichogs from the polluted site had significantly lower concentrations of 5-HT and its metabolite 5-HIAA in their medullas, but not cerebellums, than fish from the reference site (p < 0.05). Concentrations of DA, DOPAC and HVA were not different between populations in either part of the brain. Neurotransmitter concentrations were shown to be independent of size and therefore, presumably, age.Analysis of Hg in livers and brains of these mummichogs by atomic absorption spectrophotometry showed significant differences between the two populations for both organs. Earlier work showed that the behavioral deficit could be elicited by Hg; thus, this was the putative neurotoxic agent. However, there are other candidates which may contribute to neurotoxicity in the study site.     

Behavioral effects in mummichog larvae (Fundulus heteroclitus) following embryonic exposure to methylmercury

Embryos of the mummichog, Fundulus heteroclitus, were exposed to concentrations of methylmercury (meHg) below those that can cause morphological abnormalities. After hatching, larvae were kept in clean water and tested periodically for ability to capture prey (Artemia salina) or for their swimming performance and predator avoidance. Embryonic exposure to 10 μg/liter meHg resulted in reduced prey capture ability by early larvae, measured as total Artemia caught in 1 and 5 min. However, older larvae recovered from the behavioral deficits, perhaps owing to a compensation for early CNS insult. Fish from a chronically polluted environment (Piles Creek (PC), a tributary of the Arthur Kill), previously shown to have high tolerance to the teratogenic effects of higher concentrations of meHg, proved to be less susceptible to these behavioral effects than fish from a relatively uncontaminated site (EH). Also, the PC control larvae captured prey far more effectively than the EH larvae, although they made more miscues. When tested for swimming performance after embryonic exposure to 10 μg/liter, EH larvae had increased performance when compared to controls. This effect was also transitory. The improved swimming performance did not correspond to improved ability to avoid predation by grass shrimp (Palaemonetes pugio). The increased swimming performance may correspond to greater total activity, making them more likely to attract the predator's attention and resulting in increased capture. Thus, negative, though temporary, effects were observed in both prey capture and predator avoidance after embryonic exposure to meHg.     

Distribution and bioturbation effects of the tropical alpheid shrimp Alpheus macellarius in sediments impacted by milkfish farming

The impact of milkfish fish pens on the distribution of the alpheid shrimp Alpheus macellarius was studied in the Bolinao area, Philippines. In addition, the impact of the alpheid shrimp on sediment biogeochemistry, including organic matter mineralization, nutrient and sulfur cycling was compared in sediments affected and unaffected by fish farming. The fish farming activity had negative impact on the distribution of shrimps by reducing the abundance up to 60% in the vicinity of the net pens, and to almost absence inside the net pens. At a farm site abandoned seven month prior to sampling the abundance of shrimps was still low and sediment pools of reduced sulfur high, indicating a slow benthic recovery. The shrimp had large physical impact on the sediments, and the mineralization rates were stimulated by the presence of the shrimp in pristine sediments, whereas the fish farm impacted sites showed high mineralization rates both in sediments with and without the presence of the shrimp suggesting that the organic matter enrichment stimulated the mineralization in these low-organic sediments, whereas the shrimp had less impact. Sulfate reduction rates and in particular pools of sulfides increased in the farm impacted sediments, and sulfate reduction was an important process for organic matter mineralization (>67%) in all sediments. Although the shrimp had minor effect on the organic matter mineralization and nutrient fluxes in the farm impacted sediments, they appeared to increase the oxidation of sulfides by increasing the zone of oxidized surface sediment and thereby improving the sediment conditions for recolonization after fish farming has ceased.     

Differential selection for vegetative structure between juvenile brown shrimp (Penaeus aztecus) and white shrimp (P. setiferus), and implications in predator-prey relationships

The distributions of juvenile brown shrimp, Penaeus aztecus, and white shrimp, P. setiferus, (57–72 mm TL) were monitored in partially vegetated laboratory enclosures to examine selection for vegetative structure. Simulated Spartina culms, composed of green straws, were used as structure in the experiments. Brown shrimp selected for this structure during the day but not at night. White shrimp showed no strong selective preference for structure during the day or night.Selection in the field was measured by examining densities of the two species of shrimp (40–80 mm TL) in a Galveston Bay, Texas, salt marsh. Brown shrimp were more abundant in vegetated habitats (Spartina alterniflora) than in adjacent non-vegetated areas. Differences in white shrimp densities between the two habitats were variable, and no consistent pattern was observed.laboratory predation experiments were conducted in non-vegetated and vegetated cages using Atlantic croaker (Micropogonias undulatus) as predators. In non-vegetated cages there was no significant selection for either species of shrimp by the fish. In partially and completely vegetated cages, fish selected white shrimp over brown shrimp. These data suggest that the presence of vegetation alters prey selection by Atlantic croaker, and this interaction may result in greater mortality of white shrimp compared with brown shrimp in salt marshes.     

DNA strand breaks in grass shrimp embryos exposed to highway runoff sediments and sediments with coal fly ash

Embryo production was reduced in female grass shrimp exposed to sediments with added coal fly ash and to sediments collected from an estuarine station containing high PAH concentrations due to its proximity to a highway storm drain. Grass shrimp embryos exposed to pore water from the high PAH and high metal sediments showed both reduced hatching and increases in DNA strand breaks (comet assay). Sediments with added coal fly ash had high concentrations of vanadium and selenium which may have contributed to effects similar to those observed with sediments with high PAH. The embryo pore water bioassay (hatching/DNA strand breaks) gave results comparable to those observed for reproduction effects (reduced embryo production/embryo hatching) with female grass shrimp exposed to whole sediment.     

基于微卫星(SSR)分子标记的中国对虾渤海湾增殖亲虾群体及回捕群体的遗传多样性分析

Eight microsatellite markers were used to analyze genetic diversity, level of inbreeding, and effective population size of spawner and recaptured populations of Chinese shrimp(Fenneropenaeus chinensis) during stock enhancement in the Bohai Bay in 2013. A total of 254 and 238 alleles were identified in the spawner and recaptured populations, respectively, and the numbers of alleles(N_a) were 8–63 and 6–60, respectively. The numbers of effective alleles(N_e) were 2.52–21.60 and 2.67–20.72, respectively. The polymorphism information content ranged from 0.529 to 0.952. The observed heterozygosity(H_o) values(0.638–0.910 and 0.712–0.927) were lower than the expected heterozygosity(H_e) values(0.603–0.954 and 0.625–0.952), which indicated that the two populations possessed a rich genetic diversity. In 16 tests(2 populations×8 loci), 13 tests deviated from the HardyWeinberg equilibrium. F_(is) values were positive at seven loci and the inbreeding coefficients(F) of the two populations estimated by trio ML were 13.234% and 11.603%, suggesting that there was a relatively high degree of inbreeding. A certain level of inbreeding depression had occurred in the Chinese shrimp population. F_(st) values ranged from 0 to 0.059, with a mean of 0.028, displaying a low level of genetic differentiation in the two populations. Effective population sizes(3 060.2 and 3 842.8) were higher than the minimum number suggested for retaining the evolutionary potential to adapt to new environmental conditions. For enhancement activity in 2014,the ideal number of captured shrimp spawners should have ranged from 7 686 to 19 214 to maintain genetic diversity and effective population size. Further strategies to adjust the balance of economic cost, fishing effort and ideal number of shrimp spawners to maintain a satisfactory effective population size for ensuring the sustainability of Chinese shrimp are proposed.     

Pattern and annual rates of Scrobicularia plana mercury bioaccumulation in a human induced mercury gradient (Ria de Aveiro, Portugal)

Due to the lack of knowledge regarding annual bioaccumulation rates in estuarine and marine fauna, the main aim of this work was to study the annual mercury bioaccumulation in the well-documented bivalve species Scrobicularia plana along a human induced mercury gradient in the Ria de Aveiro coastal lagoon (Portugal) and in a nearby, non-polluted system (Mondego estuary), parallel to the risks associated with its consumption by humans.Minimum total mercury concentration was as low as 0.019 mg kg⁻¹ (wwt) in 4+ year old organisms in the reference site, where a significant negative correlation (p < 0.05) was found between total mercury concentrations and size, resulting in negative bioaccumulation rates (detoxification). On the other hand, values reached 1.8 mg kg⁻¹ (wwt) in 3+ year old bivalves from the most contaminated area, where a strong positive correlation with size was found (p < 0.01) and annual bioaccumulation rates were as high as 0.25 mg kg⁻¹ yr⁻¹. Annual bioaccumulation rates were highly correlated with suspended particulate matter mercury concentrations. Even though the levels of organic mercury contents increased parallel to the contamination gradient, at each sampling station, no increment was found with age, which corresponded to a decrease in organic mercury percentage with age.In terms of ecological management and public health, the ratio of 0.01 consistently found between Scrobicularia plana annual mercury accumulation rates and SPM mercury levels for most sites may permit to roughly estimate S. plana contamination of commercial sized individuals (>2.5 cm) and, if verified and confirmed in other systems, be used as a simple management tool.     

Relationship between environment and the occurrence of the deep-water rose shrimp Aristeus antennatus (Risso, 1816) in the Blanes submarine canyon (NW Mediterranean)

We performed a multidisciplinary study characterizing the relationships between hydrodynamic conditions (currents and water masses) and the presence and abundance of the deep-water rose shrimp Aristeus antennatus in a submarine canyon (Blanes canyon in the NW Mediterranean Sea). This species is heavily commercially exploited and is the main target species of a bottom trawl fishery. Seasonal fluctuations in landings are attributed to spatio-temporal movements by this species associated with submarine canyons in the study area. Despite the economic importance of this species and the decreases in catches in the area in recent years, few studies have provided significant insight into the environmental conditions driving shrimp distribution. We therefore measured daily A. antennatus catches over the course of an entire year and analyzed this time series in terms of daily average temperature, salinity, mean kinetic energy (MKE), and eddy kinetic energy (EKE) values using generalized additive models and decision trees. A. antennatus was captured between 600 and 900 m in the Blanes canyon, depths that include Levantine Intermediate Water (LIW) and the underlying Western Mediterranean Deep Water (WMDW). The greatest catches were associated with relatively salty waters (38.5–38.6), low MKE values (6 and 9 cm² s⁻²) and moderate EKE values (10 and 20 cm² s⁻²). Deep-water rose shrimp occurrence appears to be driven in a non-linear manner by environmental conditions including local temperature. A. antennatus appears to prefer relatively salty (LIW) waters and low currents (MKE) with moderate variability (EKE).