Biosorption an innovative tool for bioremediation of metal-contaminated municipal solid waste leachate: optimization and mechanisms exploration

Municipal solid waste poses a risk on surrounding environment and public health, mainly because of unscientific disposal and shortage of facilities for proper handling and recycling of leachate. This research article objective is to pinpoint the indigenous fungal isolates of waste leachate samples. Therefore, we carried out biosorption of Cd²⁺ tested the applicability by applying indigenous fungal isolates. The limited number of fungal isolates was found based on their ability for biosorption of Cd²⁺ metal. The fungal strains Trichoderma sp., Aspergillus niger and Aspergillus flavus were reported as potential strains for metal exclusion ability from the leachate. Among them, the Trichoderma sp. was found as excellent fungal agent for Cd²⁺ absorption. The optimum pH was 5.5 ± 1, temperature 45 °C, and spore concentration 10^?5 to achieve the maximum biosorption, and 35 days of incubation period were required by three strains. The maximum metal biosorption achieved was comparable for the three isolates: 56.34% by Trichoderma sp., 44.74 and 42.04% by A. niger and A. flavus, respectively. Concluding, the further intending application to identified potentially fungal isolates is able to improve the efficiency of metal biosorption. These strains are recommended for development of consortia could become a best technique for MSW leachate treatment if its reliability and applicability should be verified prior to technology acceptance.     

Soil bacterial strains with heavy metal resistance and high potential in degrading diesel oil and <Emphasis Type="Italic">n</Emphasis>-alkanes

Four bacterial strains, capable of degrading diesel oil, n-alkanes or hexadecane, were isolated from soils contaminated with petroleum oil and identified. Strains of Pseudomonas sp., Pseudomonas putida TPHK-1 and Pseudomonas aeruginosa TPHK-4, were more efficient in degrading high concentrations of the hydrocarbons than the other two strains, Stenotrophomonas maltophilia TPHK-2 and Acenitobacter sp. TPHK-3. P. putida TPHK-1 exhibited tolerance to very high concentrations of heavy metals such as cadmium, lead, zinc and copper. The innate ability of P. putida TPHK-1, as evidenced by the amplified genes alkB1 and alkB2 that encode alkane hydroxylases, and cat12o and cat23o coding for catechol dioxygenase, in degrading diesel oil in the presence of heavy metals is far greater than that of the strains reported in the literature. Heavy metal tolerance coupled with rapid degradation of hydrocarbons, even at high concentrations, suggests that P. putida TPHK-1 has a great potential in remediating soils contaminated with mixtures of hydrocarbons and heavy metals.     

<Emphasis Type="Italic">Robinia pseudoacacia</Emphasis> and <Emphasis Type="Italic">Melandrium album</Emphasis> in trace elements biomonitoring and air pollution tolerance index study

The accumulation efficiency of selected trace elements in the leaves of Melandrium album and Robinia pseudoacacia grown on heavy metal contaminated sites in comparison with a non-contaminated one was evaluated. The study was undertaken to calculate air pollution tolerance index and to determine the contents of selected metabolites: glutathione, non-protein thiols, ascorbic acid, chlorophyll and the activity of antioxidant enzymes: guaiacol peroxidase and superoxide dismutase. Such estimations can be useful in better understanding of plants defense strategies and potential to grow in contaminated environments. The results in the most contaminated site revealed higher contents of metals in M. album leaves, especially Zn, Cd and Pb (3.4, 6 and 2.3 times higher, respectively) in comparison with the R. pseudoacacia. Better accumulation capacity found in M. album was shown by metal accumulation index values. The plants could be used as indicators of Zn, Cd (both species) and Pb (M. album) in the soil. Glutathione content (in both species) and peroxidase activity (in M. album), general markers of heavy metals contamination, were increased in contaminated sites. In most cases in contaminated areas R. pseudoacacia had decreased ascorbic acid and chlorophyll levels. Opposite tendency was recorded in M. album leaves, where similar or higher contents of the above-mentioned metabolites were found. In our study, M. album and R. pseudoacacia proved to be sensitive species with the air pollution tolerance index lower than 11 and can be recommended as bioindicators.     

Microbiostratigraphy of the Upper Paleocene to Middle Eocene Jahrum Formation in the Folded Zagros Zone,SW Iran

The Jahrum Formation (Upper Paleocene to Middle Eocene) is composed of carbonate and dolomitic carbonate rocks in the Zagros Basin. The Zagros is located at the boundary between the Arabian and Eurasian lithosphere plates and represent the orogenic response to a collision between Eurasia and advancing Arabia during the Cenozoic. The study area is located in the northern part of Kuh-E-Tudej, ~175 km southeast of Shiraz in the Folded Zagros Zone. The Jahrum Formation at Kuh-E-Tudej, with a thickness of 190 m, consists of medium to massive bedded limestone. The following foraminiferal index species are identified in the studied section: Fallotella alavensis, Kathina sp., Miscellanea sp., Lockhartia sp.,Orbitolites shirazeinsis, Nummulites sp., Opertorbitolites sp., Dictyoconus cf. egyptiensis, Orbitolites cf. complanatus, Dictyoconus sp., Coskinolina sp., Somalina stefaninii, Discocyclina sp., Praerhapydionina sp., Coskinolina cf. liburnica, Nummulites cf. globulus, Nummulites cf. aturicus, and Alveolina sp. The age of the studied sediments ranges from Upper Paleocene to Middle Eocene. The microbiostratigraphic studies revealed four biozones based on the foraminifers identified in the studied section.     

Health risk of heavy metals from vegetables irrigated with sewage water in peri-urban of Dera Ismail Khan,Pakistan

Health risks of heavy metals in vegetables irrigated with sewage water were investigated in the present study. The findings indicated a massive accumulation of heavy metals in soil and vegetables collected from Dera Ismail khan, Pakistan. The concentration levels of heavy metal in vegetables grown on soil irrigated with untreated sewage water were significantly higher at (P ≤ 0.001) than in vegetables grown on fresh-water-irrigated soil and proceeded the recommended limits of World health organization. Moreover, the findings also indicated that the adults and children consuming such vegetables ingested a large proportion of the selected toxic metals. Health risk index was greater than one for Pb and Cd in all the selected vegetables and was greater than one for Ni in three vegetables like Spinacia oleracea, Benincasa fistulosa and Lactuca sativa. Health risk assessment would be a useful tool for information regarding any threats of heavy metals contamination in vegetables.     

Campanian-Lower Paleogene gastropods from the Kharga Oasis,south Western Desert,Egypt

The Campanian-Lower Paleogene successions exposed at the Kharga Oasis contain well-preserved gastropods. Nineteen gastropod species are identified and described in detail. Two new species are established: Gisortia khargaensis from the lower Eocene El Rufuf Formation and Ornopsis? barisensis from the lower Paleocene Kurkur Formation. In addition to, Turritella (Torquesia) derbesi Coquand is recorded herein for the first time from Egypt. The studied gastropods belonging to four clades: Sorbeoconcha (Metacerithium abictiforme (Wanner), Mesalia hofana Mayer-Eymar, Mesalia sp., Turritella (Torquesia) derbesi Coquand, Turritella (Torquesia) forgemoli Coquand, Turritella kaserensis Hewaidy and Azab, Turritella sp.), Littorinimorpha (Cypraedia bullina Oppenheim, Gisortia khargaensis nov. sp., Natica farafrensis Wanner, Aporrhais schweinfurthi (Quaas), Drepanocheilus bicarinatus Abbass, Drepanocheilus? sp., Anchura? unicarinatus (Abbass), Epitonium sp.), Neogastropoda (Ornopsis? barisensis nov. sp., Rostellana daniensis (Quaas), Vouta sp.), and Heterobranchia (Avellana cretacea Quaas).     

Early Triassic palynomorphs from Kuraloi Block,Belphar area,Ib-River coalfield,Mahanadi basin,Odisha

A rich assemblage of palynomorphs including miospores and megaspores has been recorded from the bore core no. IBKAN-2 drilled at a depth of 17.80m in Kuraloi Block-A, south-west part of the Ib-River Coalfield, Jharsuguda district, Odisha. The miofloral assemblage recovered shows prominence of cingulate, zonate, taeniate, non-striate and non saccate palynotaxa which indicate an early Triassic age and is comparable with the palynoassemblages of the same age known from the Damodar basin, India. The megaspore assemblage includes eight genera and fifteen species namely, Banksisporites karanpuraensis, B. indicus, B. utkalensis, Banksisporites sp., Barakarella pantii, Barakarella sp., Biharisporites spinosus, Biharisporites sp., Erlansonisporites triassicus, Erlansonisporites cf. erlansonii, Erlansonisporites sp. Jhariatriletes sp., Singhisporites sp., Ramispinatispora sp. and Talchirella trivedii. Records of megaspores are sparse in the early Triassic of Gondwana basins of India. The present study records the first occurrence of miospores and megaspores from the early Triassic of Mahanadi basin besides substantiating the earlier data.     

Phytoremediation potential and morphological changes of plants growing in the vicinity of lead smelter plant

With the increasing industrialization, heavy metals concentration in soils has greatly increased. Phytoremediation is a low-cost, non-intrusive and aesthetically harmonious technology that uses plants to remediate contaminated sites by heavy metals. The aim of the study was to determine Cd, Pb and Zn concentration in the biomass of plant species growing on a multi-metal-contaminated site of lead smelter processing, to assess the workability of using these plants for phytoremediation purposes and highlight possible damage in morphological leaf changes. Two plant species, i.e., Ipomoea asarifolia and Urochloa decumbens and the associated soil samples were collected and analyzed Cd, Pb and Zn concentrations and then calculating the bioconcentration factor and translocation factor parameters for each element. Leaves and roots samples were observed by light microscopy. Metal concentrations varied greatly and majorly depend on site sampled, plant species and tissue. Cd, Pb and Zn in tissue ranged from 0 to 102.48, 0 to 381.04 and 12.84 to 295.02 mg Kg^?1. However, none of the plant showed potential for hyperaccumulation. Both plants showed bioaccumulation factor more than one, where it was 7.66 and 6.82 for Pb and Zn in U. decumbens, respectively. Translocation factor was calculated below one for both plants and all metals. Morphological studies revealed development of adaptive features that strengthen the U. decumbens to grow in contaminated soil. Our study suggests that I. asarifolia and U. decumbens have potential for phytostabilization at multi-metal-contaminated site.     

Lower Bathonian Belemnites and Biostratigraphy of the Central and Southern Parts of the East European Platform: Part 1. Megateuthididae

Belemnites from the lower Bathonian of the Russian Plate are revised on the basis of the study of two reference sections—Pletnyovka and Sokur quarries. The first part of the study deals with the members of the family Megateuthididae. They include eight species of the genus Barskovisella gen. nov., neoendemic to the territory of the Russian Plate and originating from high Boreal taxa—species of the genus Paramegateuthis Gustomesov, 1960, which immigrated to the Middle Russian Sea in the early Bathonian via a short-lived meridional strait. The new genus includes six new species described in the present paper—Barskovisella pseudoishmensis sp. nov., B. issae sp. nov., B. variabilis sp. nov., B. barskovi sp. nov., B. gracilis sp. nov., and B. renegata sp. nov. The beds with Barskovisella, a new belemnite-based unit, corresponding to the Oraniceras besnosovi ammonite zone and including four successive phylogenetic biohorizons well comparable in resolution with ammonite-based infrazonal subdivisions, are introduced.     

Smaller foraminifers from type sections of the Bolorian stage,the Lower Permian of Darvaz

Smaller foraminifers from Upper Yakhtashian and Bolorian deposits of the stratotype area (Pamir, Darvaz, Tajikistan) are investigated. Four assemblages are defined. The first assemblage is from Chalaroschwagerina vulgaris-Pamirina darvasica Zone. The second assemblage found in the transitional Yakhtashian-Bolorian beds includes Globivalvulina, Palaeotextulariidae, Hemigordiidae, and Glomospira, associated with the first Pachyphloia and Langella forms. Characteristic taxa of third assemblage from the Misellina (Brevaxina) dyhrenfurthi Zone are Geinitzinidae, Globivalvulina, Palaeotextulariidae, Glomospira, and rare Pachyphloia. The forth assemblage of Hemigordiidae, Pachyphloia, Palaeotextulariidae, Geinitzinidae, Pseudoagathammina is identified in the M. (Misellina) parvicostata Zone. The assemblages were compared with concurrent analogs from China, Japan, and Russia. New species and subspecies Glomospira paleograndis sp. nov., G. darvasica sp. nov., Agathammina darvasica sp. nov., Pachyphloia darvasica sp. nov., Nodosinelloides cubanicus elongatus subsp. nov., and Hemigordius saranensis darvasicus subsp. nov. are described.     

Uptake of metals by plants in urban areas

In the present study, bulk contents of Ni, Zn, Cu, Pb and Mn in urban area of Tehran city are determined. Subsequently, the chemical bonds of metals with various soil fractions are brought out. Chemical partitioning studies revealed that various percentile of Ni, Zn, Cu, Pb and Mn is found in anthropogenic portion of soils. Zinc, Ni, Cu, Pb and Mn fall within “low pollution” class in accordance with index of pollution (I _POLL). The trend of anthropogenic share of studied metals in soils of Tehran is Zn (55 %) > Cu (31 %) > Ni and Pb (30 %) > Mn (12 %). The overall potential of studied plants in metal removal from soil is Salvia > Viola > Portulaca. It should be pointed out that roots have higher potential in metal removal from soil when compared with leaf and stem. Lithogenic portion of metals remains intact before and after pot analysis. Thus, phytoremediation is highly dependent on the chemical bonds of metals. Present study showed that metal contents of loosely bonded ions, sulfide bonds and organometallic bonds are reduced after 90 days of plant cultivation. The overall removal trend of studied metals is Zn (16 %) > Cu (14 %) > Ni (11 %) > Pb (7 %) > Mn (6 %). The obtained results show that the anthropogenic portion of metals is reduced after the phytoremediation practice. For instance, the initial anthropogenic portion of Zn (55 %) is changed to 39 % showing an overall reduction of about 16 %. The anthropogenic portions of Cu, Ni, Pb and Mn are also reduced by 14, 11, 7 and 6 %, respectively.     

Late Carboniferous lycopsids of the Karantrav locality (the Southern Urals)

Numerous plant remains as the imprints of lycopsid strobili and stems and the sporadic imprints of articulate stems were first found in the Upper Carboniferous deposits from the Western Slope of the Southern Urals (Karantrav Settlement area). The brief stratigraphic characteristic of the studied locality is given. The studied plant assemblage is represented by Lepidodendron ophiurus Brongniart, L. vaselgense Anikeeva et O. Orlova, sp. nov., Lepidodendron sp., Lepidostrobus tevelevii O. Orlova, Mamontov et Anikeeva, sp. nov., L. ronnaensis Bek et Oplustil, Knorria sp., Calamites sp., and Mesocalamites ramifer (Stur) Hirmer. In-situ microspores of the Lycospora type were discovered in the sporangia of some strobili of the genus Lepidostrobus. Four lycopsid species (two are new) were described.     

The Bajocian–Bathonian plant fossil from the Hojedk formation,Lenjan section,Kerman, Iran

The Jurassic successions represent a wide distribution in North of the Kerman province. These successions include Ab-Haji, Badamu, and Hojedk formations. The Hojedk Formation contains the plant fossils. The Lenjan section is one of the suitable areas for paleontological studies on the Hojedk Formation. The study section is mostly composed of green sandstone and shale with several interbedded coal veins with different thicknesses. The thickness of the Hojedk Formation is about 200 m in the Lenjan section. In this study, seven genera and 13 species of macro plant fossils were identified and described, including Nilssonia undulata, Nilssonia bozorga, Nilssonia berriesi, Nilssonia sp., Klukia cf. exilis, Klukia exilis, Cladophlebis antarctica, Coniopteris lobata, Coniopteris murrayana, Elatocladus confertus, Podozamites sp., Equisetites sp., and Coniopteris sp. The Bajocian–Bathonian can be attributed to the Lenjan section based on the recognized flora.     

Evaluation of the use of legumes for biodegradation of petroleum hydrocarbons in soil

The aim of this research was to evaluate the potential of six legumes: Medicago sativa L., Glycine max, Arachis hypogea, Lablab purpureus, Pheseolus vulgaris and Cajanus cajan to restore within a short period of time soil contaminated with 3% crude oil. The legumes in five replications were grown in crude oil-contaminated and crude oil-uncontaminated soil in a completely randomized design. Plants were assessed for seedling emergence, plant height and leaf number. GC–MS was used to analyze the residual crude oil from the rhizosphere of the legumes. Plant growth parameters were reduced significantly (P < 0.05) for legumes in contaminated soil compared to their controls. In the 4th week after planting (WAP), shoot height increased across the species up to the 8th WAP. However, in the 12 WAP no significant increase in the shoot of all species was observed. Two WAP legumes planted in contaminated soil had significantly (P < 0.05) higher leaf number than these planted in uncontaminated soil with the exception of M. sativa. In the 4th WAP, only A. hypogea and P. vulgaris had increased leaf number, while in the 6th WAP, only L. purpureus had increased leaf number and survived up to the 12th WAP while most of the legumes species died. Chromatographic profiles indicated 100% degradation of the oil fractions in C. cajan and L. purpureus after 90 days. For other legumes however, greater losses of crude oil fractions C₁–C₁₀ and C₁₀–C₂₀ were indicated in rhizosphere soil of P. vulgaris and G. max, respectively. The most effective removal (93.66%) of C₂₁–C₃₀ components was observed in G. max-planted soil even though vegetation was not established. The legumes especially C. cajan, L. purpureus and A. hypogea are promising candidates for phytoremediation of petroleum hydrocarbon-impacted soil.     

Identification of diverse bacterial communities for potential bio-aids capable of troubleshooting for wastewater biological treatment processes

Three commercial bio-aid samples have shown good troubleshooting capabilities earlier during several biological wastewaters processing, but their microbial contents were still unclear. This study was aimed to assess eubacteria contents for the three bio-aids based on sequence comparisons of 16S ribosomal deoxyribonucleic acid (rDNA). Majority of target 16S rDNA sequences of bacteria within or cultured from bio-aids were obtained via pyrosequencing-based approach, and few sequences were from isolation- or cloning-sequencing approaches. Results showed that a total of 8114, 2327, and 5402 sequences were retrieved from pyrosequencing-based methods for the three bio-aids, respectively, which were affiliated with 100, 80, and 59 bacterial species based on sequence comparison using Ribosome Data Project (RDP) Classifier and Basic Local Alignment Search Tool (BLAST). Target sequences matched Bacillus sp., Enterococcus sp., and Clostridium sp. of Firmicutes phylum were the most abundant for the bio-aids, while some sequences identical to bacteria of phyla Actinobacteria, Bacteroidetes, Chloroflexi, Cyanobacteria, and Proteobacteria were also found. Additionally, most of sequences from the isolation- or cloning-sequencing-based methods were not retrieved by the pyrosequencing-based methods. Culture media appeared to potentially select several bacteria species within phyla Firmicutes and/or Proteobacteria, but sequences from the culture-based methods did fulfill some absences for the uncultured method. Based on principal coordinate analysis, bacteria community structures of the three bio-aids were different. This study provided insights into bacteria contents for the three bio-aids as fundamental information in attempts for future selection or formulation of suitable bio-aids for solving various troubles during wastewater biological processing.     

In situ formation of AgNPs on <Emphasis Type="Italic">S. cerevisiae</Emphasis> surface as bionanocomposites for bacteria killing and heavy metal removal

Novel bionanocomposites, S. cerevisiae–AgNPs, were synthesized by in situ formation of AgNPs on S. cerevisiae surface using fulvic acids as reductants under simulated sunlight. S. cerevisiae–AgNPs were characterized using UV–Vis spectroscopy, scanning electron microscope, transmission electron microscope and Fourier transform infrared spectroscopy. These analyses showed that AgNPs were distributed on the surface of S. cerevisiae. The application of S. cerevisiae–AgNPs in bacteria killing and heavy metal removal was studied. S. cerevisiae–AgNPs effectively inhibited the growth of E. coli with increasing concentrations of S. cerevisiae–AgNPs. E. coli was killed completely at high concentration S. cerevisiae–AgNPs (e.g., 100 or 200 µg mL^?1). S. cerevisiae–AgNPs as excellent heavy metal absorbents also have been studied. Using Cd²⁺ as model heavy metal, batch experiments confirmed that the adsorption behavior fitted the Langmuir adsorption isotherms and the Cd²⁺ adsorption capacity of S. cerevisiae–AgNPs was 15.01 mg g^?1. According to adsorption data, the kinetics of Cd²⁺ uptake by S. cerevisiae–AgNPs followed pseudo second-order kinetic model. Moreover, S. cerevisiae–AgNPs possessed ability of different heavy metals’ removal (e.g., Cr⁵⁺, As⁵⁺, Pb²⁺, Cu²⁺, Mn²⁺, Zn²⁺, Hg²⁺, Ni²⁺). The simulated contaminated water containing E. coli, Cd²⁺ and Pb²⁺ was treated using S. cerevisiae–AgNPs. The results indicated that the bionanocomposites can be used to develop antibacterial agents and bioremediation agents for water treatment.     

<Emphasis Type="Italic">Suraqalatia brasieri</Emphasis> n.gen., n.sp. (larger foraminifera) from the Maastrichtian of Sulaimani area in northern Iraq

Suraqalatia brasieri n.gen., n.sp. from the family Dicyclinidae Loeblich and Tappan 1964 occurs on the Maastrichtian carbonate platform of northern Iraq. The new genus is recognizable by its large very compressed conical test, up to 55–70 mm in diameter, to 0.3–1.6 mm in thickness, planspiral cooling having very small proloculus in the initial part and later circular chambers including numerous chamberlets with an agglutinated wall. Suraqalatia brasieri n.gen., n.sp. is associated with textulariids, miliolids and rotaliids as Loftusia elongata Cox, L. morgani Douvillé, Orbitoides medius d’Archiac, O. megaloformis Papp & Kupper, O. gruenbachensis Papp, O. apiculatus Schlumberger, Omphalocyclus macroporus (Lamarck), Siderolites calcitrapoides Lamarck, Sirtina orbitoidiformis Brönnimann & Wirz. The associated macrofauna comprises large and rich giant rudists (Preradiolites sp.), other bivalves (Gryphaea sp. and Glycymeris sp.), gastropods (Acteonella sp.), echinoderms and corals. The fauna indicates shallow marine carbonate platform conditions within the Maastrichtian green house. It is also worth mentioning that the new genus has only been recorded from the Maastrichtian age.     

Distribution,enrichment and sources of trace metals in the topsoil in the vicinity of a steel wire plant along the Silk Road economic belt,northwest China

The West Development Program, initiated in 2000 by the central government of China, has attracted huge investments in the arid and semiarid regions of northwest China. As a consequence of this development, environmental pollution and ecological degradation have been widely reported. The Silk Road economic belt proposed by China promotes further economic development in the regions, but rational planning and regular monitoring are essential to minimize any additional negative impacts of the anthropogenic activities. This article reports an investigation on the distribution, enrichment and sources of trace metals in the topsoil in and around the Ningxia Hengli Steel Wire Plant (HSWP) situated along the Silk Road economic belt. The concentrations of Cd, Pb, Cr, Cu, Zn, Ni, Mn, V and Co in the surface soils of the study area vary, respectively, in the following ranges: 0.083–18.600, 21.9–2681.0, 58.0–100.0, 14.6–169.9, 59.0–4207.3, 19.3–40.8, 411–711, 55.2–76.6 and 7.46–25.21 mg/kg. The concentrations of Cd, Pb, Cr, Cu, Zn and Co are significantly higher than their local background values. Pollution levels of these trace metals in the surface soils were assessed using contamination index (C _f ⁱ ), geo-accumulation index (I _geo), modified contamination degree (mC _d) and pollution load index. The potential ecological risks caused by the metal pollution were assessed by means of potential ecological risk factor (E _f ⁱ ) and potential ecological risk index. The Spearman correlation and cluster analysis were applied to determine the contamination sources. The HSWP zone, associated with very high potential ecological risk caused by Pb and Cd, is more seriously contaminated by trace metals than the residential zone. This study indicates that Cd, Pb, Cu, Zn and Co mainly originate from industrial pollution, whereas Cr, Mn, Ni and V result from both industrial activities and natural processes.     

Tunisian landfill leachate treatment using <Emphasis Type="Italic">Chlorella</Emphasis> sp.: effective factors and microalgae strain performance

The potential of the autoclaved Tunisian landfill leachate treatment using microalgae (Chlorella sp.) cultivation was investigated in this study. Landfill leachate was collected from Borj Chakir landfill, Tunisia. A full factorial experimental design 2² was proposed to study the effects of the incubation time and leachate ratio factors on the organic matter removal expressed in chemical oxygen demand (COD) and ammoniacal nitrogen (NH₄─N) and on the biological response of Chlorella sp. expressed by the cell density and chlorophyll content. All experiments were batch runs at ambient temperature (25 ± 2 °C). The Chlorella sp. biomass and chlorophyll a concentrations of 1.2 and 5.32 mg L^?1, respectively, were obtained with 10% leachate spike ratio. The obtained results showed that up to 90% of the ammoniacal nitrogen in landfill leachate was removed in 10% leachate ratio spiked medium with a residual concentration of 40 mg L^?1. The maximum COD removal rate reached 60% within 13 days of incubation time indicating that microalgae consortium was quite effective for treating landfill leachate organic contaminants. Furthermore, with the 10% leachate ratio spiked medium, the maximum lipid productivity was 4.74 mg L^?1 d^?1. The present study provides valuable information for potential adaptation of microalgae culture and its contribution for the treatment of Tunisian landfill leachate.