Assessment of nitrogen maintenance requirement in juvenile Black Porgy Acanthopagrus schlegeli

Nitrogen balance method and nitrogen-free diet were used in this study to determine nitrogen maintenance requirement (NM) and nitrogen maintenance requirement per unit metabolism body weight (NM’) of black porgy Acanthopagrus schlegeli. Fish with body weight (BW) of 50, 80, 120, 160 and 200 g were fed by the diets containing three graded levels of crude protein (380, 420 and 460 g/kg). The results from nitrogen balance experiment showed that the amount of nitrogen deposition varied from 0.15 to 0.31 mg/g BW...     

Effects of Dietary Protein Level on Growth and Utilization of Protein and Energy by Juvenile Mangrove Red Snapper （ Lutjanus argentimaculatus ）

A feeding trial was conducted in a recirculating water system to investigate the effects of dietary protein levels on growth, feed utilization, hepatosomatic index and liver lipid deposition of juvenile red snapper, Lutjanus argentimaculatus (average initial wet weight 8.0 ± 0.39 g and total length 3.14 ± 0.3 cm). In the experiment, six fishmeal-based diets were formulated to contain various protein levels (20% to 45% in 5% increments), with dietary energy ranging from 2210.7kJ lOOg to 2250.2kJlOOg dry matter. The protein to energy ratios of diets ranged from 8.58 mg protein kJ⁻¹ to 20.03 mg protein kJ⁻¹. Diets were fed for 90d to triplicate groups of fish stocked in 0.128m³ seawater tanks, 25 individuals each. The daily ration of 2% wet body weight was offered to the fish thrice a day. The fish at the end of the study had more than ten-fold (77.0g) increase in weight compared to the initial (8.0g). Fish fed diets of 40% and 45% protein produced significantly (P<0.05) higher weight gain of 77.2g and 76.5g, and specific growth rate (SGR) of 2.65% and 2.62% than those of 67.0 g and 68.3g, and 2.49% and 2.51% of the other diets. The broken-line regression of SGR against dietary protein level yielded an optimum dietary protein requirement of 42.6% (Y=−1.6295 + 0.1114 X ²,P<0.05). Survival remained 100% among groups. Feed conversion ratio decreased from 0.45 for fish fed 20% dietary protein to 0.35 for fish fed 45% dietary protein. Nitrogen intake increased with an increase in dietary protein, which in turn resulted in an increase in nitrogen gain of fish whole body. Fish fed 40% and 45% protein diets showed higher (P<0.05) nitrogen gain (0.27g and 0.26g) than those (0.23g and 025g) fed all other diets. Gross energy intake (GEI) in fish fed 45% protein was lower (600.67kJ) than that (607.97 kJ) of 40% protein diet, though the differences were not statistically significant (P>0.05); GEI ranging from 677.31 kJ to 663.20 kJ at remaining four diets (20% to 35% protein) did not appear to differ significantly (P>0.05). The highest energy gain of 518.33 kJ was obtained with fish fed 40% protein, resulting in the highest energy retention efficiency of 85.26%. The hepatosomatic index of fish fed diets of 20%, 25%, 30% and 35% protein were significantly (P<0.05) higher (2.09% to 2.57%) than those (1.44% and 1.41%) of fish fed diets containing 40% and 45% protein. Liver lipid contents decreased from 8.72% to 7.0% in fish fed dietary protein of 20% to 45% in 5% increments. Results suggest that the diet containing 40% to 42.6% protein with a P/E ratio of 17.6 mg protein kJ⁻¹ is required for good growth of L. argentimaculatus weighing between 8.0 g and 85.2 g under the culture conditions of the present study.     

Effects of diet and body size on phosphorus utilization of Liza haematocheila T. ＆ S.

A 21-d laboratory experiment was conducted to study, the phosphorus （P） utilization of two different diets by redlip mullet Liza haematocheila T. ＆ S. Sand-filtered water in salinity 30 and temperatare 25℃ was used. Twenty-nine fish individuals were divided into three groups： 11 to group 1 （G1） fed on diet 1, 11 to group 2 （G2） fed on diet 2, and 7 to contrast group. Diet 1 was a commercial feed, more valuable in nutrition than diet 2 that similar to natural detritus. The results show the intake phosphorus （IP） of G1 was significantly higher than that of G2, and both increased linearly with body size at a certain amount of diet. The retention phosphorus （RP） in fish of G1 was lower than G2. The relationship between retention phosphorus and body size was positive and stronger in G2. Significant difference in faecal phosphorus （FP） was found between G1 and G2. Body size significantly impacted the excretion phosphorus （EP） in G1 but G2. The loss of intake phosphorus in G1 was 10.83-20.27 mg per g fish weight gain, higher than that in G2 for 6.63-9.56. Of the phosphorus, about 10% was allocated into growth, 50% in faeces, and the rest lost in excretion. The main part of phosphorus was lost in faeces but excretion. The phosphorus budget of the fish could be described as 100IP = 7.40RP ＋ 47.39FP ＋ 36.63EP （Diet 1） or 100IP = 11.93RP ＋ 56.64FP ＋ 21.76EP （Diet 2）.     

Effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta

The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion ratio and protein efficiency rate. Nitrogen retention was found to be the highest in fish fed the diet containing 1.06% of phosphorus; however, this was not significantly different from other diets. Fish fed the diet containing 0.93% of phosphorus showed the highest phosphorus retention; similar phosphorus retention rates were found in fish fed the diets containing 0.77% and 1.06% of phosphorus. Fish fed the diet containing the highest percentage of dietary phosphorus were found to contain the least whole body lipid, lower than fish fed other diets (P<0.05). The protein content increased from 18.59% to 19.55% (although not significant) with the decrease of body lipid content (P>0.05). The contents of the whole body ash, whole body phosphorus and vertebrae phosphorus increased with dietary phosphorus percentage up to 1.06 (P<0.05), reaching a plateau after that. Dietary phosphorus did not significantly influence the muscle components (protein, lipid and moisture). Condition factor and hepatosomatic index were the highest in fish fed the diet containing 0.63% of dietary phosphorus; however, this was not significantly different from those of other diets. The second-order polynomial regression of phosphorus retention against dietary phosphorus identified a breakpoint at 0.88% of dietary phosphorus. However, the dietary requirement of phosphorus for maintaining maximum phosphorus storage determined by broken-line analysis of the contents of whole body phosphorus, and ash and vertebrae phosphorus was 1.06% of the diet.     

Effects of Dietary Protein Level on Growth and Utilization of Protein and Energy by Juvenile Mangrove Red Snapper (Lutjanus argentimaculatus )

1Introduction The mangrove red snapper,Lutjanus argentimacu-latus(Forsskal,1775)is a carnivorous,warm-watereuryhaline fish that is considerably cultured in South-east Asia,Southern China and the Middle East(Le-ung et al.,1999;Estudillo et at.,2000;Ng et al.,2000;Catacutan et al.,2001).In Pakistan,it isknown for its good quality meat and also for its highconsumption rate.Although it fetches a premiumprice at local markets(Anonymous,2002),the in-creasing demand has generated interest towar…     

Dietary leucine requirement for juvenile large yellow croaker <Emphasis Type="Italic">Pseudosciaena crocea</Emphasis> (Richardson, 1846)

Dietary leucine requirement for juvenile large yellow croaker, Pseudosciaena crocea Richardson 1846 (initial body weight 6.0 g ± 0.1 g) was determined using dose-response method. Six isonitogenous (crude protein 43%) and isoenergetic (19 kJ g⁻¹) practical diets containing six levels of leucine (Diets 1–6) ranging from 1.23% to 4.80% (dry matter) were made at about 0.7% increment of leucine. Equal amino acid nitrogen was maintained by replacing leucine with glutamic acid. Triplicate groups of 60 individuals were fed to apparent satiation by hand twice daily (05:00 and 17:30). The water temperature was 26–32°C, salinity 26–30 and dissolved oxygen approximately 7 mg L⁻¹ during the experimental period. Final weight (FW) of large yellow croaker initially increased with increasing level of dietary leucine but then decreased at further higher level of leucine. The highest FW was obtained in fish fed diet with 3.30% Leucine (Diet 4). FW of fish fed the diet with 4.80% Leucine (Diet 6) was significantly lower than those fed Diet 4. However, no significant differences were observed between the other dietary treatments. Feed efficiency (FE) and whole body composition were independent of dietary leucine contents (P > 0.05). The results indicated that leucine was essential for growth of juvenile large yellow croaker. On the basis of FW, the optimum dietary leucine requirement for juvenile large yellow croaker was estimated to be 2.92% of dry matter (6.79% of dietary protein).     

东太平洋赤道公海茎柔鱼生物学特性

根据2013年4~6月在赤道公海探捕期间采集茎柔鱼样本,研究赤道公海茎柔鱼渔业生物学特性。结果表明,不同性别、不同月份,茎柔鱼胴长和体质量组成不同。雌、雄性样本的胴长范围分别为93~495 mm和94~406 mm,优势胴长组分别为260~340 mm和240~320 mm,分别占样本总数72.53%和70.26%;雌、雄性样本体质量范围分别为25~3 900 g和33~2170 g,优势体质量组分别为400~1 200 g和200~1 000 g,分别占样本总数82.71%和87.82%。雌、雄性茎柔鱼净体质量比例分别介于31.69%~80.51%和37.05%~81.35%之间,平均值分别为60.45%和62.73%。协方差分析表明,茎柔鱼胴长与体质量、胴长与净体质量的生长均存在性别间差异,胴长与体质量的生长都最适合用幂函数表示,而胴长与净体质量的生长,雌、雄性样本分别最适合用幂函数和指数函数表示。不同月份茎柔鱼性别比例构成不同,4月、5月和6月性别比例分别为1.87∶1、1.61∶1和1.17∶1,全部样本的性别比例为1.61∶1。不同月份茎柔鱼性腺成熟度的组成不同。不同性别、不同月份间茎柔鱼胃含物等级组成存在差异。     

Effects of diet and body size on phosphorus utilization of Liza haematocheila T.& S.

A 21-d laboratory experiment was conducted to study,the phosphorus (P) utilization of two different diets by redlip mullet Liza haematocheila T.& S.Sand-filtered water in salinity 30 and temperature 25℃ was used.Twenty-nine fish individuals were divided into three groups:11 to group 1 (G1) fed on diet l,11 to group 2 (G2) fed on diet 2,and 7 to contrast group.Diet 1 was a commercial feed,more valuable in nutrition than diet 2 that similar to natural detritus.The results show the intake phosphorus (IP) of G1 was significantly higher than that of G2,and both increased linearly with body size at a certain amount of diet.The retention phosphorus (RP) in fish of G1 was lower than G2.The relationship between retention phosphorus and body size was positive and stronger in G2.Significant difference in faecal phosphorus (FP) was found between G1 and G2.Body size significantly impacted the excretion phosphorus (EP) in G1 but G2.The loss of intake phosphorus in G1 was 10.83-20.27mg per g fish weight gain,higher than that in G2 for 6.63-9.56.Of the phosphorus,about 10% was allocated into growth,50% in faeces,and the rest lost in excretion.The main part of phosphorus was lost in faeces but excretion.The phosphorus budget of the fish could be described as 100IP=7.40RP+47.39FP+36.63EP (Diet 1) or 100IP=11.93RP+56.64FP+21.76EP (Diet 2).     

Disease resistance and humoral immunomodulatory effects of vitamin C on grouper,Epinephelus awoara

The disease resistance and humoral immunomodulatory effects of vitamin C administered orally to grouper,Epinephelus awoara maintained on a frozen fish diet supplemented with vitamin C at 500, 1000, 1500 and 2000 mg/kg were investigated. After 20 weeks, the growth rates of the groups with high level of vitamin C apparently increased. The untreated fish had symptoms of vitamin C deficiency. The endogenous liver tissue vitamin C levels were found to reflect well the dietary treatments. After intraperitoneal injection or bath challenge with a virulent strain ofVibrio vulnificus, fish fed with high level vitamin C showed significantly higher survival rate compared with the normal control group. Vaccination with formalin inactivatedV. vulnificus significantly enhanced the specific antibody production in fish treated with vitamin C, and completely protected from strong bacterial challenge the groups fed on fish with vitamin C 1500 and 2000 mg/kg diet. Project 39500113 supported by NSFC and also by Guangdong Science Research Foundation (Grant No. 950711).     

Effect of Dietary Phosphorus on the Growth and Body Components of Juvenile Synechogobius hasta

The effect of dietary phosphorus on the growth and body components of juvenile Synechogobius hasta was determined. Different percentages of dietary phosphorus (0.63, 0.77, 0.93, 1.06, 1.22 and 1.36) were tested by feeding the fish (body weight, 15.81 g ± 0.32 g; 20 individuals each group; 3 groups each percentage) at a surplus of 5%–10% above satiation for 35 d. Dietary phosphorus did not significantly affect the specific growth rate, feed intake, feed conversion ratio and protein efficiency rate. Nitrogen ...     

The antitumor effect of bromophenol derivatives <Emphasis Type="Italic">in vitro</Emphasis> and <Emphasis Type="Italic">Leathesia nana</Emphasis> extract <Emphasis Type="Italic">in vivo</Emphasis>

To investigate the antitumor effect of bromophenol derivatives in vitro and Leathesia nana extract in vivo, six bromophenol derivatives 6-(2,3-dibromo-4,5-dihydroxybenzyl)-2,3-dibromo-4,5-dihydroxy benzyl methyl ether (1), (+)-3-(2,3-dibromo-4,5-dihydroxyphenyl)-4-bromo-5,6-dihydroxy-1,3-dihydroisobenzofuran (2), 3-bromo-4-(2,3-dibromo-4,5-dihydroxybenzyl)-5-methoxymethyl-pyrocatechol (3), 2,2′,3,3′-tetrabromo-4,4′,5,5′-tetrahydroxy-diphenylmethane (4), bis(2,3-dibromo-4,5-dihydroxybenzyl) ether (5), 2,2′,3-tribromo-3′,4,4′,5-tetrahydroxy-6′-ethyloxymethyldiphenylmethane (6) were isolated from brown alga Leathesia nana, and their cytotoxicity were tested by MTT assays in human cancer cell lines A549, BGC-823, MCF-7, B16-BL6, HT-1080, A2780, Bel7402 and HCT-8. Their inhibitory activity against protein tyrosine kinase (PTK) with over-expression of c-kit was analyzed also by ELISA. The antitumor activity of ethanolic extraction of Leathesia nana (EELN) was evaluated on S₁₈₀-bearing mice. All compounds showed very potent cytotoxicity against all of the eight cancer cell lines with IC₅₀ below 10 μg/mL. In PTK inhibition study, all bromophenol derivatives showed moderate inhibitory activity and compounds 2, 5 and 6 showed significant bioactivity with the inhibition ratio of 77.5%, 80.1% and 71.4%, respectively. Pharmacological studies reveal that EELN could inhibit the growth of Sarcoma 180 tumor and increase the indices of thymus and spleen to improve the immune system remarkably in vivo. Results indicated that the bromophenol derivatives and EELN can be used as potent antitumor agents for PTK over-expression of c-kit and considered in a new therapeutic strategy for treatment of cancer. Supported by the National High Technology Research and Development Program of China (863 Program, No. 2007AA09Z410) and Knowledge Innovation Program of Chinese Academy of Sciences (No. KZCX2-YW-209)     

Bioaccumulation of Pb<Superscript>2+</Superscript> and its effects on growth,morphology and pigment contents of <Emphasis Type="Italic">Spirulina</Emphasis> (<Emphasis Type="Italic">Arthrospira</Emphasis>) <Emphasis Type="Italic">platensis</Emphasis>

A laboratory experiment was conducted to assess the bioaccumulation of Pb²⁺ and its effects on growth, morphology and pigment contents of Spirulina (Arthrospira) platensis. The specimen cultured in Zarrouk liquid medium was treated with various initial metal concentrations (0, 5, 10, 30, 50 and 100 μg mL⁻¹). The growth of S. platensis was adversely affected by Pb²⁺ at high concentrations (30, 50 and 100 μg mL⁻¹). However, at low concentrations (5 μg mL⁻¹), Pb²⁺ could stimulate its growth slightly. The pigment contents (chlorophyll α and β carotene) were decreased in a dose-dependent manner. The highest reductions (67% and 53% respectively in chlorophyll α and β carotene) were observed in 100 μg mL⁻¹ treatment group. The LC₅₀ (96 h) of Pb²⁺ was measured as 75.34 μg mL⁻¹. Apart from a few cases of filament breakages at elevated concentrations (50 and 100 μg mL⁻¹), morphological abnormalities are not specific. Metal bioaccumulation increased with Pb²⁺ concentrations, but decreased with exposure time. The maximum accumulated amount was 188 mg g⁻¹ dry weight. The bioconcentration factor (BCF) reached to a peak at day 2, followed by a gradual reduction for all the exposure concentrations. S. platensis is able to tolerate considerably high Pb²⁺ concentrations. Consequently it can be used as a potential species to remove heavy metal from contaminated waters.     

Analysis of SSRs derived from an EST library of half-smooth tongue sole <Emphasis Type="Italic">Cynoglossus semilaevis</Emphasis>

A complementary DNA (cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag (EST) database was generated, containing 3100 cDNA clones, of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats (SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats (51.4%) are the most abundant class of SSR in the dataset. The dimeric, trimeric, tetrameric and hexameric repeats are represented in decreasing proportions of 27.2%, 16.0%, 2.8% and 1.9%, respectively. The frequency of pentameric repeats was observed the least (only 0.7%). Most of the monomeric and dimeric repeats are distributed in 3′ and 5′ un-translation region. If translation regions are considered merely, trimeric repeats are the highest, accounting for 57% of the total microsatellites.     

Effects of dietary soy isoflavones on feed intake, growth performance and digestibility in juvenile Japanese flounder (Paralichthys olivaceus)

An 8-week feeding trial was conducted to investigate the effects of dietary soy isoflavones on feeding intake,growth performance,and digestion of juvenile Japanese flounder(Paralichthys olivaceus).Four isonitrogenous(49% crude protein) and isoenergetic(20.1 MJ kg-1) diets were formulated to contain four graded levels of soy isoflavones,namely,0,1,4 and 8 g soy isoflavones in 1 kg of diet.Each diet was randomly fed to triplicate tanks of fish(Initial average weight:2.58 g ± 0.01 g),and each tank was stocked with 35 fish.No significant difference was observed among diets with levels of 0,1 and 4 g kg-1 soy isoflavones in feed intake,weight gain,feed efficiency ratio(FER),proximate composition of fish whole body and apparent digestibility coefficients(ADC) of nutrients and energy(P>0.05).However,high dietary soy isoflavones level(8 g kg-1) significantly depressed weight gain,FER,whole-body crude lipid content of fish and ADC of nutrients(P<0.05).These results indicate that high level of dietary soy isoflavones(above 4 g kg-1) significantly depresses growth responses and FER of Japanese flounder.However,as the content of soy isoflavones in soybean meal is around 1 to 3 g kg-1,the adverse effects might be neglected when soybean products are used as a fish feed ingredient.     

Metabolism of polychaete Neanthes japonica Izuka： relations to temperature, salinity and body weight

Polychaete Neanthes japonica is a species geographically specific in China and Japan with important scientific implication and commercial value. In this study, the relations of body weight, salinity and temperature to oxygen consumption and ammonia excretion of N. japonica were determined. Three different groups in body weight (large: 2.34±0.36 g, middle: 1.50±0.21 g and small: 0.62±0.12 g) were set for all experiments. Results show that the body weight is negatively related to the rates of oxygen consumption and ammonia excretion; and the relationship is significant. The oxygen consumption and ammonia excretion at 24°C decreased at salinity from 5 to 30 and increased above 30, indicating that both lower and higher salinity are adverse and certain degree of salinity stress is necessary for enhancing the energy demand. At salinity 30, rising temperature from 18°C to 30°C, the oxygen consumption increased before 27°C and then decreased. However, the relation of ammonia excretion and temperature seems more complex. Two-way ANOVA shows that salinity, temperature and body weight all have a significant effect on the oxygen consumption and ammonia excretion of the worm. Moreover, interaction between salinity/temperature and body weight is also significant. O:N (oxygen/nitrogen) ratio varies greatly in this case from 5.97 to 463.22, indicating that N. japonica can regulate the type of metabolic substrate against environment changes. Supported by the National Natural Science Foundation of China (No. 30490233) and the Three-Gorge Project and the Estuarine Environment (No. SX2004-018) from the Three Gorges Project Construction Committee     

摄食水平对卵形鲳鲹幼鱼的生长和能量收支的影响

水温28±1℃条件下,投喂人工配合饲料,研究摄食水平1%、2%、3%、4%、5%(每日投喂量占初始实验鱼湿重的质量百分比)及饱食对卵形鲳鲹幼鱼(初始体重5.77±0.45g)生长及能量收支的影响。结果表明,卵形鲳鲹幼鱼特定生长率、转化效率、摄食率随着摄食水平的提高而增长,摄食水平达4%时干重特定生长率与饱食组差异无显著性(P>0.05),摄食水平达5%时,湿重、蛋白质和能量生长率与饱食组差异无显著性(P>0.05);摄食水平达4%时能值转化效率与饱食组差异无显著性(P>0.05),摄食水平达5%时,干重、蛋白质转化效率与饱食组差异无显著性(P>0.05);摄食水平达2%时干重、蛋白质表观效率与饱食组差异无显著性(P>0.05),摄食水平达4%时,能量表观效率与饱食组差异无显著性(P>0.05)。生长能分配率随摄食水平升高而显著增加(P<0.05),代谢能、排粪能及排泄能分配率的变化则相反。建立了6种摄食水平条件下卵形鲳鲹幼鱼的能量收支方程。     

Water Intake and Utilization in Mithun （Bosfrontalis）： Effect of Environmental Temperature,Rearing System and Concentrate Feed Supplement

Seasonal and sexual variations as well as the effect of dry feed supplement on total drinking water intake and its utilization were observed in mithun （Bosfrontalis） - a semi-wild animal found in North Eastern Hill Region （NEHR） of India. In a completely randomized design, twelve adult mithuns （B. frontalis） as per their sex and body weight were assigned in two different rearing systems （free grazing and free grazing with dry concentrate feed supplementation）, and ten growing male mithuns as per their body weight assigned in two different levels of dry concentrate feed supplementation （1.o kg and 2.0 kg dry concentrate feeds on green forage based diet） and in two different seasons （summer and winter）. It was observed that the environmental temperature had a significant effect on drinking water intake by mithuns. Drinking water consumption （per unit of body weight） was significantly （P 〈 0.05） higher in summer than in winter. Supplementation of concentrate feed on free grazing animals resulted in increase in water consumption. Total water consumption （drinking as well as performed water） was found to be 15.18 litres per 100 kg body weight by growing mithun. Feed dry matter and digestible nutrient intakes by growing mithun were observed to be increased with the increase of supplementation of dry concentrate feed. Roughage to concentrate ratio did not affect the nutrient digestibility. Mithun calves drank an average of 4.30 litres water for each kg of dry matter intake. Metabolic water was significantly （P〈0.01） increased with the increase of supplementation of concentrate feed whereas water turn over, which depends upon the body weight of the animals, did not differ significantly on offering of lower or higher level of dry feed. Faecal water loss of growing mithun was decreased with the increase in intake of concentrate feed and was estimated to be 33 - 46 % of total water intake. Excretion of water through faeces of mithun was about 3.8 % of body weight. It could, there     

Effects of supplemental coated or crystalline methionine in low-fishmeal diet on the growth performance and body composition of juvenile cobia Rachycentron canadum （Linnaeus）

We evaluated the effects of supplemental coated and crystalline methionine （Met） on the growth performance and feed utilization of juvenile cobia （Rachycentron canadum Linnaeus） in a 60-d feeding trial. Fish groups were fed one of six isonitrogenous and isolipidic diets： 1） fishmeal control; 2） un- supplemented experimental （low-fish-meal diet deficient in Met）; or 3） one of four Met diets supplemented with crystalline L-Met, cellulose-acetate-phthalate coated L-Met, acrylic-resin coated L-Met, or tripalmitin- polyvinyl alcohol coated L-Met. The test diets were fed to triplicate groups of cobia （initial body weight 5.40-4-0.07 g） twice a day. The weight gain and specific growth rate of the fish fed the RES diet were highest among the Met-supplemented groups and were 23.64% and 7.99%, respectively, higher than those of the fish fed with the un-supplemented experimental diet （P〈0.05）. The protein efficiency ratio of the fish fed the MET diet was significantly higher than that of the fish fed the un-supplemented experimental diet and the fish in the other methionine supplementation groups （P〈0.05）. Our results suggest that supplementation of crystalline Met in low-fish-meal diets promotes the growth performance of juvenile cobia.     

广东流沙湾网箱养鱼区水体氮磷的时空变化及负荷估算

研究流沙湾网箱养鱼区水体氮、磷含量的月变化和水平分布,并运用质量平衡方程估算其氮、磷负荷。结果表明:总氮变化在0.212~0.892 mg/L之间,平均0.498 mg/L;总磷变化在0.009~0.036 mg/L之间,平均0.020mg/L;氮磷比变化在23.6~26.1之间,平均为25。在5-9月,氮、磷含量变化呈逐渐增加趋势,到9月达到最大,10月又减少。网箱内部氮、磷的含量高于网箱外部,并随着与网箱距离的增大而逐渐减小。红鳍笛鲷(Lutjanus argentimaculatus)、紫红笛鲷(Trachinotus ovatus)和卵形鲳鲹(Lutjanus erythopterus)全鱼的氮质量分数平均为2.42%,磷质量分数平均为0.26%,饵料系数为6.67,流沙湾网箱养鱼养殖期产生的氮、磷负荷平均分别为135.8和19.4 kg/t。     

EFFECTS OF FISH OIL,DHA OIL AND LECITIHIN IN MICROPARTICULATE DIETS ON STRESS TOLERANCE OF LARVAL GILTHEAD SEABREAM（SPARUS AURATA）

The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.