疣荔枝螺(Thais clavigera Kuster)人工繁育技术研究

本实验开展了疣荔枝螺（Thais clavigera Kuster）室内全人工繁育技术研究；具体包括亲螺人工促熟蓄养、卵囊采集、孵化、幼虫培育、变态和采苗等技术研究，同时，还开展了后期面盘幼虫对不同附着基的喜好选择性实验，以及不同饵料和光照对幼虫附着的影响研究。研究表明：雌螺分批产卵，单个雌螺平均每次产出受精卵为61 750粒；在水温27~29℃时，面盘幼虫经过20 d左右的生长发育，壳长达到约600 μm，此时开始附着变态，由浮游生活转变为底栖生活；附着后10 d左右变态为稚螺。后期面盘幼虫对附着基的选择性实验表明，固着牡蛎苗的栉孔扇贝壳和附有底栖硅藻的波纹板，是稚螺理想的附着基。不同饵料和光照对幼虫附着影响实验结果表明：在附着变态期间，自然光照有利于幼虫附着变态；同时，连续投喂糠虾肉糜可显著提高幼虫变态率和稚螺成活率。本研究结果为今后疣荔枝螺产业化繁育提供了重要的理论和技术支持。     

象拔蚌人工育苗技术研究

通过对亲贝促熟、人工催产、幼虫培育和采苗等技术环节的研究,建立了象拔蚌(Panopea gen-erosa)的人工繁育技术。研究表明,在盐度28~30条件下,受精卵在水温11、13、16oC时发育至D形幼虫所需时间分别为83、60和46 h;在水温16~18oC,经20 d发育至壳长约380μm的壳顶幼虫时出现足和水管原基,进入附着变态阶段。投放附着基后培育约70 d,可生长至壳长5 mm的幼贝。壳长日增长量在D形幼虫阶段为6.2μm,在壳顶幼虫阶段为16.8μm,附着变态后生长速度加快,外形具成体形态。不同附着基的采苗率依次为：细砂>卵石>聚乙烯网片>波纹板。     

几种化学物质对两种牡蛎幼虫附着和变态的诱导

本文研究5种神经递质和1种神经肽对两种牡蛎幼虫附着和变态的诱导效应。结果表明：L－多巴对牡蛎幼虫附着行为的诱导快速而有效，浓度10μmol/dm^3，2h内有附着行为的幼虫约占50％，并使之正常变态，肾上腺素、去甲肾上腺素和L－多巴均能有效地诱导两种牡蛎幼虫变态，它们的最适诱导浓度分别为50、50和5μmol/dm^3，诱导变态率分别达97．1％、90．6％和70．5％（长牡蛎），74．9％、74．3％和37．2％（僧帽牡蛎）；多巴胺的诱导变态效果不显著，而γ－氨基丁酸和5－羟色胺不能有效诱导变态。两种牡蛎幼虫对上述有效诱导药物的反应，长牡蛎优于僧帽牡蛎。肾上腺素诱导长牡蛎，最适处理时机在眼点幼虫后期，持续处理时间为2h之内。     

微生物粘膜对杂色鲍幼虫附着和变态的影响

为了探讨广泛存在于海中基质表面的微生物粘膜对鲍幼虫附着和变态的诱导作用,研究了不同年龄微生物粘膜 ( 1 ～ 6 d ) 对杂色鲍幼虫附着和变态的影响.结果表明:1 ～ 6 d微生物粘膜均显著地诱导幼虫附着,其中 3, 5 和 6 d 微生物粘膜还显著地诱导幼虫变态;幼虫的附着率和变态率分别与微生物粘膜中的藻类密度和细菌密度呈显著的正相关性,推测藻类和细菌可能在幼虫的附着和变态中起着重要作用.垂直基质选择性试验结果表明,90 % 以上的幼虫附着在具 23 d 微生物粘膜的载玻片表面且变态率达 70 % 以上,进一步证实了微生物粘膜可以诱导杂色鲍幼虫的附着和变态,同时也证实了幼虫具备选择附着基质的能力.     

底栖硅藻生物膜附着基对扇贝幼虫附着和变态的影响

为了建立生态、高效的扇贝幼虫附着和变态诱导技术,采用底栖硅藻生物膜附着基对栉孔扇贝和海湾扇贝开展了附着和变态诱导的现场实验。实验围绕底栖硅藻在扇贝幼虫培育池内的数量变动、存活状态、在栉孔扇贝食谱组成中的贡献以及其对两种扇贝附着和变态的诱导效果开展。结果表明,底栖硅藻附着基能极显著提高海湾扇贝和栉孔扇贝幼虫的附苗量和变态率(P<0.01)。在海湾扇贝实验中,底栖硅藻处理组比对照组附苗量提高220.19%(P<0.01),变态率和壳长两组差异不显著(P>0.05)。在栉孔扇贝实验中,底栖硅藻处理组比对照组附苗量提高43.02%(P<0.01),变态率提高87.31%(P<0.01),底栖硅藻处理组壳长和对照组差异不显著(P>0.05)。对照组附着基比对照组早3d检查到变态的稚贝。底栖硅藻附着基在进入幼虫培育池的黑暗环境后光合作用受限,对于扇贝幼虫的日常管理导致底栖硅藻脱落,数量有一定的下降,但丰度最终能保持为56.0-183.9个/mm2。使用基于混合模型对栉孔扇贝稚贝食物来源进行分析结果显示金藻Isochrysis galbana对稚贝的食物贡献较高,底栖硅藻的贡献较低,其0.95水平的置信区间的贡献率为0%-44%,表明底栖硅藻也是扇贝物来源之一。本研究为底栖硅藻生物膜在贝类幼虫附着变态过程中作用的研究奠定了良好的基础,并为生态、高效的商业化苗种培育提供理论和技术支撑。     

大西洋浪蛤(Spisulasolidissima)人工繁殖 初步试验

1999年5～6月对大西洋浪蛤进行人工繁殖试 验。结果表明,大西洋浪蛤一年能达到性成熟,一个繁殖季节产卵一次,卵为球形,卵径为 50～60μm;在23℃左右水温下,受精卵经16～20h发育成D形幼虫,幼虫浮游期为16～20d; 壳长260～290μm时为附着变态阶段。幼虫培育盐度要求在16以上。     

海洋无脊椎动物幼虫附着变态研究进展 II:附着变态模型及人工诱导物在经济贝类苗种生产中的应用

１海洋无脊椎动物幼虫附着变态机理模型目前为止 ,对一些重要经济贝类如鲍和牡蛎的附着变态机理研究比较深入 ,已经到达神经生物学和细胞生物学水平。目前 ,有关海洋无脊椎动物幼虫附着变态机理模型主要有３个 :以太平洋牡蛎（Ｃｒａｓｓｏｓｔｒｅａｇｉｇａｓ）幼虫附着变态为代表的双调控模型、以红鲍（Ｈａｌｉｏｔｉｓｒｕｆｅｓｃｅｎｓ）幼虫附着变态为代表的上行调节模型以及以多毛类Ｐｈｒａｇｍａｔｏｐｏｍａｃａｌｉｆｏｒｎｉｃａ幼虫附着变态为代表的脂肪酸调控模型。Ｂｏｎａｒ等１９９０年和Ｃｏｏｎ和Ｆｉｔｔ等１９９０年…     

大竹蛏的繁殖生物学

2005年1月至2007年12月,采用组织学和实验生态学方法对大竹蛏(Solen grandis Dunker)的性腺发育、生殖周期、肥满度、胚胎发育、幼虫发育及变态等进行了研究.结果表明,大竹蛏性腺发育过程分为增殖期、生长期、成熟期、排放期、休止期5个阶段;在浙南沿海大竹蛏繁殖期为4月下旬至5月中旬(水温21～24℃);肥满度最高出现在5月份,为31.2%;最低出现在2月份,为21.2%.大竹蛏卵径为85～95 μm;受精卵在水温22℃,经20～24 h孵化成D形幼虫;初孵D形幼虫平均大小为125 μm,浮游幼虫经5～6 d培养进入附着变态期,壳长为250 μm,发育变态为稚贝.     

温度和盐度对海湾扇贝幼虫附着及变态的影响

本文研究了在室内控制温度和盐度的条件下,海湾扇贝幼虫附着与变态的适宜范围和最适范围;幼虫附着、变态时间及附着行为与温、盐度的关系等。     

氯化钾诱导仿刺参(Apostichopus japonicus)幼虫附着变态的研究

以不同浓度的氯化钾(KCl)溶液处理仿刺参(Apostichopus japonicus)樽形幼虫,处理不同时间后,分析KCl对仿刺参幼虫附着变态的诱导作用。结果表明,使用较低浓度的KCl溶液(10、30和50 mmol/L)分别诱导仿刺参樽形幼虫6、12和24 h均可以显著提高其附着变态率,诱导24 h附着变态率分别提高27.67%、32.67%和19.67%。较高浓度的KCl溶液(100、300和500 mmol/L)也可以诱导仿刺参幼虫附着变态,但随着KCl浓度的升高和诱导时间的延长,幼虫死亡率逐渐升高。低浓度KCl溶液,特别是在30 mmol/L诱导仿刺参幼虫12 h时,可以显著提高幼虫的附着变态率,增加发育的同步性,降低死亡率,对仿刺参幼虫变态的诱导效果较好。     

鲐鱼灯光围网渔船水上集鱼灯水中照度分布及优化配置的理论计算

采用叠加法照度计算模式,根据一组鲐鱼灯光围网渔船中3艘不同渔船的水上集鱼灯布置参数,使用自行开发的水上集鱼灯水下光场计算系统,对各船的水中照度分布进行了计算,获得船舷右侧中部水深方向200 m范围内不同深度的照度,并使用Surfer 8.0软件绘制等值曲线图,结果表明:主灯船在总功率为180 kW的情况下,10 lx的等照度曲线水平方向最远在56 m左右,水深方向不超过20 m;离船水平距离40 m、垂直距离40 m处的照度约为0.01 lx。副灯船在总功率为120 kW的情况下,10 lx的等照度曲线水平方向最远在46 m左右,水深方向不超过18 m;离船水平距离33 m、垂直距离38 m处的照度约为0.01 lx。网船在总功率为40 kW的情况下,10 lx的等照度曲线水平方向最远在45 m左右,水深方向不超过15 m;离船水平距离30 m、垂直距离35 m处的照度为0.01 lx左右。从集鱼灯的配置情况来看,主灯船、副灯船与网船目前的配置均较好,但主灯船在灯距增大到0.26 m、灯高增大到5 m时可增加1.6%的有效水体体积,副灯船在灯距减少到0.28 m、灯高增大到5 m时可增加2.1%的有效水体体积,网船在灯距增大到0.64 m、灯高增大到9 m时可增加1.7%的有效水体体积。计算结果还表明,大幅度的增加光诱渔船的集鱼灯功率并不能很有效的提高该船的光诱范围。     

Distribution of Living Larger Foraminifera NW of Sesoko-Jima, Okinawa, Japan

Abstract. Living, symbiont-bearing, larger foraminiferids restricted to the photic zone incorporate mechanisms blocking solar radiation and water current transportation. They can be divided in two groups according to their wall structure: 1, Families with opaque test walls, reducing light penetration: Peneroplids inhabit shallow-water environments from the intertidal ( Peneroplis in large numbers) down to 40 m ( Dendritina , preferring sandy substrates). Soritids are restricted to subtidal environments with less water turbulence and range from the reef moat down to 60 m. Amphisorus and Marginopora are common down to 30 m, whereas Parasorites is restricted to deeper parts of the reef slope. Alveolinella can be found in the upper 40 m of the reef slope. 2. Families with hyaline test walls, adjusted for light penetration: amphisteginids inhabit the whole photic zone, demonstrating a correlation of test flattening with increasing depth. Calcarinids cling with their spines to firm substrates such as algal thalli or macroids. Baculogypsina is restricted to high energetic regions on the reef flat. Calcarina inhabits similar environments, extending its distribution range within fore reefs down to 80 m. Baculogypsinoides is a dominant calcarinid genus from 30 to 70 m depth. Within nummulitids, Heterostegina prefers hard substrates on the reef slope, while Assilina (= Operculina ) is frequent in deeper parts and is independent of substrates. Sandy bottoms from 30 to 60 m are inhabited by the last living representative of the genus Nummulites, N. venosus . The giant foraminifer Cycloclypeus , with test sizes up to 7 cm, is restricted to fore reef areas below 50 m down to the base of the photic zone.     

Age at first breeding and change in plumage of kelp gulls Larus dominicanus in South Africa

In South Africa, kelp gulls Larus dominicanus first breed when 4 years old. At least 50% of 4-year-olds breed; virtually all older birds breed annually. Most chicks leave natal colonies in the austral autumn and winter, when about 6 months old, and 60% of them may not return until aged 2 or 3 years. One 3-year-old showed signs of defending territory, but birds only attain full adult plumage when aged 4. The plumage of 3-year-olds is similar to that of adults, but the white spots on the outer primaries are less well developed and the secondaries and inner primaries have no white trailing edge. Chicks are dark, whereas birds aged 1 and 2 years gain increasing amounts of pale or white plumage on the face, neck, underparts and tail.     

Extracting energy while reducing hydroelastic responses of VLFS using a modular raft wec-type attachment

This paper presents the use of a modular raft Wave Energy Converter (WEC)-type attachment at the fore edge of a rectangular Very Large Floating Structure (VLFS) for extracting wave energy while reducing hydroelastic responses of the VLFS under wave action. The proposed modular attachment comprises multiple independent auxiliary pontoons (i.e. modules) that are connected to the fore edge of the VLFS with hinges and linear Power Take-Off (PTO) systems. For the hydroelastic analysis, the auxiliary pontoons and the VLFS are modelled by using the Mindlin plate theory while the linear wave theory is used for modelling the fluid motion. The analysis is performed in the frequency domain using the hybrid Finite Element-Boundary Element (FE-BE) method. Parametric studies are carried out to investigate the effects of pontoon length, PTO damping coefficient, gap between auxiliary pontoons, and incident wave angle on the power capture factor as well as reductions in the hydroelastic responses of the VLFS with the modular attachment. It is found that in oblique waves, the modular attachment comprising multiple narrow pontoons outperforms the corresponding rigid attachment that consists of a single wide pontoon with respect to the power capture factor and the reduction in the deflection of the VLFS. In addition, it is possible to have a considerable gap between pontoons without significantly compromising the effectiveness of the modular attachment.     

不同树龄桐花树茎上白条地藤壶分布特征的研究

研究了广西英罗港红树林区向海林带不同树龄的桐花树茎上白条地藤壶的分布特征。结果表明，白条地藤壶在茎上的附着高度（h)随树龄和树高增大而增大。在最大树龄17龄植株上平均达到182cm，此高度为潮高约512cm海水才能浸及，在一年中约有将近90％的时间暴露在空气中。附着高度（h)与树高（H）比值h/H以5龄树最大，达91．9％。树层最大密度达46600Ind/m^2，最小为172Ind/m^2。在同一树龄的不同树层，密度表现为先随树高增大逐渐增大，达到最大密度后又下降。在不同树龄的同一树层，密度高低变化，分布一般出现多个峰。白条地藤壶最适宜附着和生长在桐花树茎上100－120cm树层（浸及此树层的海水潮高约为430－450cm)。树层最大生物量为1719．05g/m^2，最小为5．39g/m^2。生物量分布与密度的相似。     

氧含量及附着面积对海洋底栖硅藻生长和胞外多聚物的影响

胞外多聚物(extracellular polymeric substance, EPS)是海洋底栖硅藻在水下表面附着时分泌的物质,环境因素对胞外多聚物的组成和分泌行为有直接影响,本文重点探讨了氧含量和附着面积对胞外多聚物的影响。采用热溶剂浸提法提取硅藻胞外多聚物,利用苯酚-硫酸法定量其中的多糖成分,利用考马斯亮蓝G-250法定量蛋白质成分,通过血球计数板法和紫外分光光度法计算细胞数量。结果表明,在氧含量高、可附着面积大的条件下,硅藻的生长繁殖速率快,且单个细胞的胞外多聚物分泌量大。氧含量对胞外多聚物主要成分中的水溶性多糖含量影响大,而对其余成分含量影响小。可附着面积对硅藻附着和生长的影响比氧含量影响小。     

闽南—台湾浅滩渔场多毛类的分布

1987年12月至1988年11月在闽南-台湾浅滩渔场获得83种多毛类，隶属于35科67属。欧努菲虫（Onuphis eremita）、锥唇吻沙蚕（Glycera o-nomichiensis）、拟特须虫（Paralacydonia paradoxa）和滑指矶沙蚕（Eunice indica）等出现频率较高。马氏独毛虫（Tharyx marioni）、奇异稚齿虫（Paraprionospio pin-nata）、多齿全刺沙蚕（Nectoneanthes multignatha）和梯额虫（Scalibregma inflatum）密集地分布于局部水域。闽南-台湾浅滩渔场多毛类年平均生物量为1.13g/m^2，年平均密度为25个/m^2，均低于台湾海峡北部、东山湾湖下带和大亚湾等水域。闽南-台湾浅滩渔场多毛类表现为低多样性特征。这些分布特征取决于底质类型，该海区沉积颗粒较粗，不利于多毛类繁衍。海流和水团也有重要作用。     

长江口12.5米深水航道南坝田挡沙堤加高工程总平面方案研究

长江口12.5米深水航道2010年贯通后,发挥了巨大社会经济效益,同时航道回淤量大、维护压力大、维护费用高的问题突出。本研究基于北槽四边界水沙通量观测成果,分析提出了北槽航道回淤泥沙来源;针对回淤原因,在已建减淤工程经验总结的基础上,提出了本次减淤的研究思路,优化了减淤工程方案的比选指标体系;采用三维潮流泥沙数模、清水动床物模、经济技术综合分析等手段,通过"加高范围"、"加高高程"及"加高位置"比选,研究推荐了减淤工程方案。利用实测回淤量分析了工程减淤效果。研究结果表明,南导堤越沙是洪季北槽的重要泥沙来源,对北槽高浓度含沙量场有一定贡献。提出了可通过加高北槽南侧的导堤,实现减少通过南导堤越堤进入北槽的泥沙量,从而减小北槽含沙量水平,同时改善北槽下段流态,降低水沙横向输移,进而降低航道回淤的减淤思路。研究推荐的长江口12.5米深水航道减淤工程为南坝田挡沙堤加高工程及先期工程方案。先期工程位于S4～S9丁坝坝田,在现有南坝田挡沙堤的基础上加高S4～S8区段,并延长至S9丁坝,工程全长约23.8 km,高程+3.5 m。工程于2015年11月开工建设,2016年7月主体工程完工,工程减淤效果显著,2016—2018年年均减淤量约954×105m3/a,近三年已节省航道维护疏浚费用约5亿元。     

Cold deep water in the South China Sea

Two deep channels that cut through the Luzon Strait facilitate deep (>2000 m) water exchange between the western Pacific Ocean and the South China Sea. Our observations rule out the northern channel as a major exchange conduit. Rather, the southern channel funnels deep water from the western Pacific to the South China Sea at the rate of 1.06 ± 0.44 Sv (1 Sv = 10⁶ m³s⁻¹). The residence time estimated from the observed inflow from the southern channel, about 30 to 71 years, is comparable to previous estimates. The observation-based estimate of upwelling velocity at 2000 m depth is (1.10 ± 0.33) × 10⁻⁶ ms⁻¹, which is of the same order as Ekman pumping plus upwelling induced by the geostrophic current. Historical hydrographic observations suggest that the deep inflow is primarily a mixture of the Circumpolar Deep Water and Pacific Subarctic Intermediate Water. The cold inflow through the southern channel offsets about 40% of the net surface heat gain over the South China Sea. Balancing vertical advection with vertical diffusion, the estimated mean vertical eddy diffusivity of heat is about 1.21 × 10⁻³ m²s⁻¹. The cold water inflow from the southern channel maintains the shallow thermocline, which in turn could breed internal wave activities in the South China Sea.     

Field Observation and Analysis of Wave-Current-Sediment Movement in Caofeidian Sea Area in the Bohai Bay, China

In order to study the mechanism of flow-sediment movement, it is essential to obtain measured data of water hydrodynamic and sediment concentration process with high spatial and temporal resolution in the bottom boundary layer (BBL). Field observations were carried out in the northwest Caofeidian sea area in the Bohai Bay. Near 2 m isobath (under the lowest tidal level), a tripod system was installed with AWAC (Acoustic Wave And Current), ADCP (Acoustic Doppler Current Profilers), OBS-3A (Optical Backscatter Point Sensor), ADV (Acoustic Doppler Velocimeters), etc. The accurate measurement of the bottom boundary layer during a single tidal period was carried out, together with a long-term sediment concentration measurement under different hydrological conditions. All the measured data were used to analyze the characteristics of wave-current-sediment movement and the BBL. Analysis was performed on flow structure, shear stress, roughness, eddy viscosity and other parameters of the BBL. Two major findings were made. Firstly, from the measured data, the three-layer distribution model of the velocity profiles and eddy viscosities in the wave-current BBL are proposed in the observed sea area; secondly, the sediment movement is related closely to wind-waves in the muddy coast area where sediment is clayey silt: 1) The observed suspended sediment concentration under light wind conditions is very low, with the peak value generally smaller than 0.1 kg/m3 and the average value being 0.03 kg/m3; 2) The sediment concentration increases continuously under the gales over 6-7 in Beaufort scale, under a sustained wind action. The measured peak sediment concentration at 0.4 m above the seabed is 0.15-0.32 kg/m3, and the average sediment concentration during wind-wave action is 0.08-0.18 kg/m3, which is about 3-6 times the value under light wind conditions. The critical wave height signaling remarkable changes of sediment concentration is 0.5 m. The results show that the suspended load sediment concentration is mainly influenced by wave-induced sediment suspension.