水质酸化对双棘臂尾轮虫种群动态的影响

用人工酸化的东湖水为培养液对双棘臂尾轮虫进行室内单个体培养研究。结果表明,与用未酸化的东湖水培养结果相比,轮虫非混交雌体的平均产卵量在ｐＨ６．５,５．５和４．５时增加,而在ｐＨ３．５时减少在ｐＨ５．５和３．５时轮虫种群的内禀增长率增大,种群中无混交雌体出现;而在ｐＨ６．５和４．５时内禀增长率减小,混交雌体百分率增大。     

密度和温度对萼花臂尾轮虫产卵量和混交雌体的影响

用小球藻(Chlorella sp.)为食培养萼花臂尾轮虫(Brachionus calyciflorus),食物浓度约1×10~6cells/mL,温度25℃,光照强度约40001x,昼长比LD=18:6。在1.0mL、0.5mL和0.25mL培养液中同时进行单个体和群体培养。结果发现:1)单个体培养时每个母体平均产卵量分别为12.0,13.8和7.8个;后代个体中混交雌体的百分比分别是46.38％,53.49％和55.83％。2)群体培养时,每个母体平均产卵量分别为8.7、3.1和2.65个,随密度增加而减少;后代个体中混交雌体百分比分别为41.70％,53.59％和54.26％,群体培养的密度为4.0ind./mL与2.0ind./mL,2.0ind./mL与1.0ind./mL混交雌体的百分比差异不明显。母体密度高低与后代混交雌体百分比间具有明显相关性。在上述培养条件下,轮虫为1.0ind./mL时,进行单个体培养,分别用急剧冷休克(SS)、逐渐冷休克(GS)和恒温对照(C)进行处理,结果表明:个体平均产卵量分别为10.8、9.2和12.6个。后代混交雌体百分比分别为32.85、20.7和16.87,SS和C处间后其混交雌体比例具显著差异,而SS和GS,GS和C处理间没有显著差异。     

培养液更换对萼花臂尾轮虫(Brachionus calyciflorus)休眠卵形成的影响

在种群累积培养状态下研究了培养液更换对萼花臂尾轮虫（ Ｂｒａｃｈｉｏｎｕｓ ｃａｌｙｃｉｆｌｏｒｕｓ）休眠卵形成的影响,结果表明,在培养液不予更换的情况下,萼花臂尾轮虫休眠卵的产量和形成效率分别为 ７２４ ３３３３± ４０． ５２５７ｉｎｄ（６ｄ．２０ｍＬ）－１和 ６ ．０３６１± ０． ３３７７ｉｎｄ（５ × １０６ ｃｅｌｌｓ）－１（ａｌ－ｇａｅ ｄｉｅｔ）与之相比,当培养液每 ２天更换一次时,其休眠卵的产量和形成效率分别下降至６３７．３３３３±１６． ８０２８ｉｎｄ．（６ｄ·２０ｍＬ）－１和５ ．３１１１±０ ．１４００ｉｎｄ（５×１０６ｃｅｌｌｓ）－１（ａｌｇａｅ　ｄｉｅｔ）．随着培养液更换频率增大至每天一次时,其休眠卵的产量和形成效率下降的幅度进一步增大培养液更换对轮虫种群中的混交雌体百分率和混交雌体受精率无显著的影响,最后探讨了培养液更换对萼花臂尾轮虫休眠卵形成影响的机理．     

粉煤灰污染影响萼花臂尾轮虫(Brachionus calyciflorus)种复合体空间分布的原因

为探讨粉煤灰污染影响萼花臂尾轮虫(Brachionus calyciflorus)种复合体在灰湖、汀棠湖和凤鸣湖分布的生态学机制,本文研究了曝气自来水(对照)、20％、40％、60％、80％和100％的澄清灰湖水对采自三湖泊中的轮虫姐妹种Ⅰ和汀棠湖中的轮虫姐妹种Ⅱ的种群增长率、混交雌体数/非混交雌体数、携卵雌体数/不携卵雌体数和休眠卵产量的影响.结果显示,无论实验用水中的澄清灰湖水含量如何,来自三湖泊的轮虫姐妹种Ⅰ均具有较高的种群增长率(0.52～0.85 d-1)和休眠卵产量(1.60～12.11ind./(4d·5ml)),这为轮虫的扩散、种群的建立和种群间的基因交流提供了可能.汀棠湖轮虫姐妹种Ⅱ种群中的混交雌体数/非混交雌体数和休眠卵产量均极低,对照组中的混交雌体数/非混交雌体数和休眠卵产量均为0,这是存在于汀棠湖和凤鸣湖中的轮虫姐妹种Ⅱ无法通过扩散而出现在灰湖中的主要原因.澄清灰湖水的含量仅对汀棠湖中两轮虫姐妹种的种群增长率具有显著的影响.各浓度的澄清灰湖水使轮虫姐妹种Ⅰ的种群增长率显著高于对照组,60％、80％和100％的澄清灰湖水使轮虫姐妹种Ⅱ的种群增长率显著高于对照组,这表明粉煤灰污染导致已报道的相关水体中轮虫种群密度的下降并非由于其中所含的污染物(较高浓度的碱性氧化物、硫化物和金属元素等)和水体pH值的改变等对轮虫的直接毒性作用.     

武汉东湖沉积物中轮虫休眠卵的分布

报道了１９９２年８月￣１９９３年７月间武汉东湖营养类型不同的３个湖区沉积物中轮虫休眠的卵数量和分布。不论湖区营养类型如何,沉积物中的休眠卵数量均呈“Ｖ”形垂直分布,表层高,底层低,由表向底数量逐渐递减。在３个湖区中,休眠卵的数量随水体营养水平的增高而增加。营养水平最高的Ⅰ站其休眠卵数量年平均为１．３２个／ｃｍ＾３,营养水平较低的Ⅱ、Ⅲ站分别为０．７４个／ｃｍ＾３、０．７１个／ｃｍ＾３。休眠卵的数量     

一秋龄性成熟中华绒螫蟹的生物学──1.外部形态特征及性腺变化

本文描述了一秋龄性腺成熟中华绒螯蟹（简称河蟹）的外部特征并与一秋龄未成熟及二秋龄成熟河蟹进行比较,同时给出了体重与壳宽的回归方程．保安湖的研究结果表明,对于一秋龄雌蟹,体重大于２９．８ｇ（壳宽为４．０３ｃｍ）全部成熟,而体重小于１３．１ｇ（壳宽为３．００ｃｍ）均不成熟,体重在１３、１－２９．８ｇ之间,既有成熟又有未成熟．对于一秋龄雄蟹,体重大于２６．０ｇ（壳宽３．９１ｃｍ）,全部成熟,而于１２．０ｇ壳宽２．６６ｃｍ）均未成熟,体重在１２．０－２６．０ｇ之间,既有成熟者又有未成熟个体．对一秋龄成熟河蟹成熟系数逐月变化的观察表明,在翌年３月份达到峰值,雌蟹为１２． ３２± １． ７５％,雄蟹为 ４． ２４± ０． ０６％;此后,性腺迅速退化,至翌年七月上旬,卵巢退变成细索状结构．己无完整的卵粒．     

富蕴地震断裂带的若干定量特征

对富蕴地震断裂带９处不同地段的平板地质测量结果表明：北部引张段地震断裂 带宽约２２０ｍ,断坎平均高０．４ｍ;中部主体走滑段地震断层长达５３５ｍ以上,断层水平位移为１．８￣７．８ｍ断坎高０．４￣１．２ｍ,水平位移与垂直位移之比为５．７￣８．６;南部末端破裂段地震抛掷巨石距离达４０ｍ,地震断层水平位移为０．４￣９．９ｍ,断坎平均高０．２ｍ,末端及副断层水平位移与垂直位移之比高达２５￣２８。     

洪湖日本沼虾的种群繁殖生物学

１９９４年５月至１９９５年６月对洪湖日本沼虾的种群繁殖生物学进行了研究。洪湖日本沼虾的繁殖期为４月中旬至１０月上旬,５月中旬至８月中旬为日本沼虾的繁殖高峰期,其中６月、８月雌虾抱卵率均在７０％以上;日本 虾群体中雄性总是少于雌性,月性比（♀／♂）变化于１．１０４－５．７８０之间,３－６月性比逐渐增高,７－１０月性比则逐渐降低;在整个繁殖期,４－７月的抱卵是均为前一年出生的大、中规格的个体,之后当年     

广东沿海地区现代地壳垂直运动研究

对３０多年来１万多ｋｍ精密水准复测数百个重合点资料进行了综合研究，结果表明，广东沿海岸段的地壳平均以－０．５ｍｍ／ａ幅度沉降，表现出粤西抬升，粤东沉降，珠江三角洲大面积下沉的格局。粤西岸段平均以＋０．６ｍｍ／ａ速率上升，粤东沿海岸段平均以－０．７ｍｍ／ａ速率下降；珠江三角洲以－１．８ｍｍ／ａ速率下沉，桂东南岸段则以＋３．４ｍｍ／ａ的速上升。     

区域地震台网的动态监测能力

提出了一种区域台网监测能力的动态估计方法,结果表明,平均台距减小１倍,台风密度增加至４倍,但台网监测能力仅提高０．３￣０．４级,为使监测能力提高１级,平均台距需减至１／８,台网密度则增大６４倍,若台站短周期仪器放大率一律为５万倍,每万平方公里１个台的平均台网密度,其台网可监测最小震级为ＭＬ２．０（ｍｌｇ标度）。     

草鱼、团头鲂对伊乐藻的消化与利用

本文分析了伊乐藻的生化成分和能值,测定了草鱼、团头鲂对伊乐藻生化成分的消化率,草鱼对伊乐藻的最大摄食量、蛋白质利用率和饲料系数。结果表明,伊乐藻含水率为89.8％,干物质中粗蛋白质、粗脂肪、粗纤维素和无氮浸出物含量分别为23.52％、2.88％、26.71％和29.27％,含有17种常见氨基酸,总量为198.04mg/g(干重)。在平均水温20.4℃的室外条件下,草鱼,团头鲂对伊乐藻的干物质消化率分别为56.02％、54.97％,对粗蛋白质消化率分别为79.05％、74.96％,对氨基酸总量的消化率均为77.00％,对粗脂肪消化率分别为73.96％,64.63％,对碳水化合物消化率分别为58.46％、51.36％。在伊乐藻饲养试验中,草鱼每天最大摄食量(Y)与试验鱼体重(X)相关显著,相关系数为0.9154,关系式为Y=3.9449X~(0.8357)。在网箱中饲养草鱼,伊乐藻的粗蛋白质利用率为17.1％,饲料系数为48.2。在小型围栏中饲养草鱼,伊乐藻的粗蛋白质利用率为13.1％,饲料系数为63.4。     

Calcium isotope fractionation in coccoliths of cultured Calcidiscus leptoporus, Helicosphaera carteri, Syracosphaera pulchra and Umbilicosphaera foliosa

Four species of marine calcifying algae, the coccolithophores Calcidiscus leptoporus, Helicosphaera carteri, Syracosphaera pulchra and Umbilicosphaera foliosa were grown in laboratory cultures under temperatures varying between 14 and 23 °C, and one species, C. leptoporus, under varying [CO₃²⁻], ranging from 105 to 219 μmol/kg. Calcium isotope compositions of the coccoliths resemble in both absolute fractionation and temperature sensitivity previous calibrations of marine calcifying species e.g. Emiliania huxleyi (coccolithophores) and Orbulina universa (planktonic foraminifera) as well as inorganically precipitated CaCO₃, but also reveal small species specific differences. In contrast to inorganically precipitated calcite, but similar to E. huxleyi and O. universa, the carbonate ion concentration of the medium has no statistically significant influence on the Ca isotope fractionation of C. leptoporus coccoliths; however, combined data of E. huxleyi and C. leptoporus indicate that the observed trends might be related to changes of the calcite saturation state of the medium. Since coccoliths constitute a significant portion of the global oceanic CaCO₃ export production, the Ca isotope fractionation in these biogenic structures is important for defining the isotopic composition of the Ca sink of the ocean, one of the key parameters for modelling changes to the marine Ca budget over time. For the present ocean our results are in general agreement with the previously postulated and applied mean value of the oceanic Ca sink (Δ_sed) of about − 1.3‰, but the observed inter- and intra-species differences point to possible changes in Δ_sed through earth history, due to changing physico-chemical conditions of the ocean and shifts in floral and faunal assemblages.     

黑藻(Hydrilla verticillata)对铜绿微囊藻(Microcystis aeruginosa)抑制作用

采用黑藻(Hydrilla verticillata)和铜绿微囊藻(Microcystis aeruginosa)共同培养的实验方法,追踪测定微囊藻的生长量、光合速率等相关生理生化指标以及观察藻细胞超微结构变化,研究黑藻对铜绿微囊藻的抑制作用及其机制．结果表明,黑藻对铜绿微囊藻的生长有明显的抑制作用,表现为藻细胞生长量显著降低,细胞超微结构进行性损伤直至细胞解体,藻体叶绿素a含量和光合速率急剧下降,呼吸速率、超氧物歧化酶(SOD)活性均呈现先升高后下降的趋势,膜脂过氧化产物丙二醛(MDA)的积累量与膜的损伤程度相一致．在黑藻养殖水中初步分离得到黑藻分泌的抑藻物质．     

The empirical relationships between M s, m b and M L for China and vicinity

Data from 753 earthquakes are used to determine a relationship between surface-wave magnitude (M _s) and bodywave magnitude (m _b), and from 541 earthquakes to determine a relationship between surface-wave magnitude (M _s) and local magnitude (M _L) for China and vicinity: M _s=0.9883 m _b-0.0420, M _s=0.9919 M _L-0.1773. The relationship of M _s versus m _b is obtained for 292 events occurred in the Chinese mainland in the time period from 1964 to 1996, 291 events occurred in Taiwan in the time period from 1964 to 1995 and 170 events occurred in the surrounding area. Standard deviation of the fitting is 0.445. Relationship of M _s versus M _L is obtained for 36 events occurred in the Chinese mainland, 293 events occurred in Taiwan, China and 212 events occurred in the surrounding area. The total amount is 541 events. Standard deviation of the fitting is 0.4673. The uncertainties of the converted M _s in different magnitude intervals can be estimated using complementary cumulative distribution function (CCDF). In the relationship of M _s versus m _b, taking ±0.25 as a range of uncertainties, in magnitude interval m _b 4.0–4.9, the probabilities for the converted M _s taken value less than (M _s-0.25) and more than (M _s+0.25) are 17% and 27% respectively. Similarly, we have probabilities for m _b 5.0–5.9 are 34% and 20% and that for m _b 6.0–6.9 are 11% and 47%. In the relationship of M _s versus M _L, if the range of uncertainties is still taken as ±0.25, the corresponding probabilities for magnitude interval M _L 4.0–4.9 are 22% and 38%, for M _L 5.0–5.9 are 20% and 15% and for magnitude interval M _L 6.0–6.9, are 15% and 29%, respectively. The relationships developed in this paper can be used for the conversion of one magnitude scale into another magnitude scales conveniently. The estimation of uncertainties described in this paper is more accurate and more objective than the usual estimation expressed by deviation. The estimations described in this paper indicate various dispersions in different magnitude intervals of original data. The estimations of uncertainties described by probabilities can be well connected with the total estimations of uncertainties in seismic hazard assessment.     

Effect of mixed toxic diets (Microcystis and Chlorella) on the rotifers Brachionus calyciflorus and Brachionus havanaensis cultured alone and together

Brachionus havanaensis is a common planktonic rotifer sometimes found together with Brachionus calyciflorus in Microcystis-infected waterbodies in México. Here, we evaluate the impact of mixed diets (dry weight basis, 0%, 25%, 50%, 75% and 100% Microcystis or Chlorella) on the growth of and competition between B. calyciflorus and B. havanaensis. In general, a higher proportion of Microcystis aeruginosa in the diet resulted in decreased population growth rate of both rotifer species. Whether grown separately or together, B. havanaensis was always numerically more abundant than B. calyciflorus, regardless of the proportion of Microcystis in the diet. However, when both species were grown together, the impact of M. aeruginosa in the diet had a more adverse effect on B. calyciflorus than on B. havanaensis. When grown alone, both rotifer species showed better population growth on a diet of 75% Chlorella and 25% Microcystis than either 100% Chlorella or Microcystis.     

路南海菜花的核型分析及其海菜花属的演化探讨

海菜花属(Ottelia Pers.)为沉水单子叶植物,只生长于淡水生境中,具有重要的生态价值、环境指示作用以及植物系统学研究意义.本文首次报道了特产于我国云南石林湖泊中的路南海菜花(Ottelia acuminata var.lunanensis H.Li)的体细胞染色体数目为22条,核型属"1B"型,核型公式2n=2x=22=18m+4sm.结合已研究的花部特征和核型资料讨论海菜花属种间的亲缘关系及演化,为海菜花(Ottelia)的利用和保护提供细胞生物学证据.     

太湖鲚鱼和鲢、鳙鱼的食物组成及相互影响分析

通过分析和比较太湖鲚鱼、鲢、鳙鱼的食物组成,研究了它们间的食物关系;并结合太湖历年鱼类渔获量资料,对渔获量变化间可能存在的相互影响进行了相关分析.结果表明:在9-12月份期间,鲢鱼、鳙鱼食物中微囊藻约占体积比的90%以上、浮游动物不足10%;用Pianka提出的公式计算鲢鱼和鳙鱼间的食物重叠系数为＞0.99,相同食物组成比例为95.40%.而同期调查0 鲚鱼食物中微囊藻约占体积的15%-35%;如不计算微囊藻仅计算浮游动物的个数组成比则为:枝角类占89.77%±13 69%,桡足类占7.84%±11.53%,轮虫占2.39%±4.95%.而简单相关、偏相关分析表明:鲚鱼和鲢、鳙鱼数量变化间的负相关关系均达到显著水平.分析认为:在富营养化条件下,太湖的鲢鱼和鳙鱼以微囊藻为主要食物;而鲚鱼和鲢、鳙鱼食物中浮游动物的种类基本相同,可能存在对浮游动物资源的激烈竞争;它们种群数量变化间的相互影响是负向的,可能存在互相抑制.     

铜绿微囊藻和四尾栅藻光合利用碳酸氢盐探讨

为了探讨羹类光合过程对碳酸氧盐的利用,研究不同pH值下铜绿微囊藻和四尾栅藻的净光合速率,并着重研究这两株藻的碳酸氢盐摄取速率.结果表明,从弱酸性到弱碱性,四尾栅藻的净光合速率都比铜绿微囊藻高.由pH值超过pH转折点后的无机碳摄取速率计算得到的碳酸氢盐摄取速率对铜绿微囊藻和四尾栅藻分别为142.14和314.08μmo1DIC/(g·h,FW),故这两株藻都可利用碳酸氢盐作为碳源进行光合,且均为强碳酸氧盐利用者;但是,四尾栅藻对碳酸氲盐的利用能力强于铜绿微囊藻.另外,与四尾栅藻相比,铜绿微囊藻的碳酸氧盐摄取速率更易受抑制剂的抑制.