EFFECT OF ARTIFICIAL REGULATIONS OF ARTEMIA n-3 HUFA CONTENT ON GROWTH AND SURVIVAL OF BLACK SEABREAM (SPARUS MACROCEPHLUS) LARVAE

ImODUCTIONStudiesonthenutritionmpuirementSoffishlarvaehavernadesomeprognaabroad,butwearenotawareofreportSonsuchstudiesinChinanow.ltwasdeter-Annedanhythatn-3HU-FA,espedally22f6(n-3)roHA)and2O5(n-3)(EPA)ises-sentialformarinefishlarvaegroWth.ThehighcontentofDHAinmarinefisheggSandthel0wcontent0fDHAinArtrmiashowthatArthetaasliwfndcandsaoothen-3HUFAreq~dmendfishlacae.bosdenistSdriitaln-3HUFAenriedArbotabyboingthemn-3HUFAcontainingbogtanulataldietS,etC.Watanabe,T.(l983)enri~livefe…     

The Response of Bio-Component Production of Nannochloris oculata to the Combinations of Monochromatic Light

Illuminating conditions are crucial factors affecting the microalgal growth and biosynthesis.The effects of illuminating spectral quality on the growth and bio-component production of Nannochloris oculata were investigated.The results indicated that a high proportion of Red-light enhanced the pigments and carbohydrate production but reduced those of the biomass and lipid.Mono-chromatic Blue-light has advantageous effects on lipid production compared with Red-and White-light.The optimal light spectrum for the protein production was the combination of Red-and Blue-light at a ratio of 4:3 or 5:2.Among the seven fatty acids identified from N.oculata,the contents of C16:0,C18:0,and C18:3(n-3)in the lipid were inhibited by the increased proportion of Red-light while those of C18:2(n-9),C16:2(n-6),and C20:0 were inhibited by Blue-light.Monochromatic Red-and Blue-light and their com-binations were proposed as a promising illuminating strategy for the large-scale cultivation aiming for various bio-components.     

大花细辛挥发油化学成分GC-MS分析

通过对大花细辛(Asarummaximum Hemsl)挥发油化学成分研究,探讨其保健和药用机理,为开发和利用大花细辛提供科学依据。用无水乙醇对大花细辛进行提取,并用GC-MS对提取物进行化学成分和相对含量分析共获得86种化合物,其中主要的并具研究价值的有33种,占总化合物的92.68%。其中5-甲基-1,2,3-三甲氧基苯(C10H14O3)的相对含量最高,为23.00%;其次为2-甲氧基-4-乙烯基苯酚(C9H10O2),为10.54%。     

Toxic hexavalent chromium reduction by Bacillus pumilis,Cellulosimicrobium cellulans and Exiguobacterium

Three bacterial strains B acillus pumilis,Cellulosimicrobium cellulans and Exiguobacterium were investigated when grown in Luria-Bertani(LB) medium at 500 μg/m L Cr(VI). The hexavalent chromium reduction was measured by growing the strains in De Leo and Ehrlich(1994) medium at 200 and 400 μg/m L K 2 Cr O 4. The optimal Cr(VI) reduction by strains B. pumilis,Exigubacterium and C. cellulans was 51%,39%,and 41%,respectively,at an initial K2 Cr O 4 concentration of 200 μg/m L at p H 3 and temperature 37°C. At an initial chromate concentration of 400 μg/m L,the Cr(VI) reduction by strains B. pumilis,Exigubacterium and C. cellulans was 24%,19%,and 18%,respectively at p H 3 at 37°C after 24 h. These strains have ability to reduce toxic hexavalent chromium to the less mobile trivalent chromium at a wide range of different environmental conditions and can be useful for the treatment of contaminated wastewater and soils.     

Optimization of dilute acid hydrolysis of Enteromorpha

Acid hydrolysis is a simple and direct way to hydrolyze polysaccharides in biomass into fermentable sugars. To produce fermentable sugars effectively and economically for fuel ethanol, we have investigated the hydrolysis of Enteromorpha using acids that are typically used to hydrolyze biomass:H2SO4, HCl, H3PO4 and C4H4O4 (maleic acid). 5%(w/w) Enteromorpha biomass was treated for different times (30, 60, and 90 min) and with different acid concentrations (0.6, 1.0, 1.4, 1.8, and 2.2%, w/w) at 121°C. H2SO4 was the most effective acid in this experiment. We then analyzed the hydrolysis process in H2SO4 in detail using high performance liquid chromatography. At a sulfuric acid concentration of 1.8% and treatment time of 60 min, the yield of ethanol fermentable sugars (glucose and xylose) was high, (230.5 mg/g dry biomass, comprising 175.2 mg/g glucose and 55.3 mg/g xylose), with 48.6% of total reducing sugars being ethanol fermentable. Therefore, Enteromorpha could be a good candidate for production of fuel ethanol. In future work, the effects of temperature and biomass concentration on hydrolysis, and also the fermentation of the hydrolysates to ethanol fuel should be focused on.     

Effect of Dietary Lipid on the Growth,Fatty Acid Composition and Δ5 Fads Expression of Abalone(Haliotis discus hannai Ino) Hepatopancreas

This study investigated the effect of dietary lipid on the growth, fatty acid composition and Δ5 fatty acyl desaturase genes(Fads) expression of juvenile abalone(Haliotis discus hannai Ino) hepatopancreas. Six purified diets were formulated to contain tripalmitin(TP), olive oil(OO, 72.87% 18:1n-9), grape seed oil(GO, 68.67% 18:2n-6), linseed oil(LO, 70.48% 18:3n-3), ARA oil(AO, 41.81% ARA) or EPA oil(EO, 44.09% EPA and 23.67% DAH). No significant difference in survival rate was observed among abalone fed with different diets. Weight gain rate(WGR) and daily growth rate of shell length(DGRSL) were significantly increased in abalone fed with diets containing OO, AO and EO, but decreased in abalone fed with LO diet(P 0.05) in comparison with those fed with TP. High level of dietary 18:2n-6 resulted in higher content of n-6 polyunsaturated fatty acids(PUFAs) in abalone fed with GO than those fed with TP, OO, LO and EO(P 0.05). n-3 PUFAs in abalone fed with LO was significantly higher than those in abalone fed with TP, OO, GO and AO(P 0.05). The highest contents of 20:1n-9 and 22:1n-9 were observed in abalone fed with OO. The expression of Δ5 Fads in hepatopancreas of abalone was enhanced by high concentration of 18:3n-3 and suppressed by dietary LC-PUFAs; however it was not affected by dietary high concentration of 18:1n-9 or 18:2n-6. These results provided valuable information for understanding the synthesis of LC-PUFAs and nutritional regulation of Δ5 Fads expression in abalone.     

西施舌生长的研究

于 1998年 11月至 2 0 0 2年 12月 ,在福建长乐对西施舌 (Coelemactraantiguata)的生物学特征进行测定 ,以人工养殖方法观察其周年生长情况。观测结果表明 ,长乐沿海西施舌的群体组成以体长 6 1～ 110mm占优势 ,其频率 ( fn)为 0 .14 0～ 0 .2 2 2～ 0 .12 5。不同大小的西施舌 ,软体部肥满度指数 (CI)具有明显的季节差异。 1龄西施舌月平均体长增长 4 .0 5mm ;2龄的为 2 .31mm ;3龄的为 1.83mm ;1～ 3龄为成体生长发育阶段 ,3龄之后趋向衰老。不同发育生长阶段的壳高与壳长直线回归关系 :幼虫期H =0 .994L - 13.0 7;稚贝期H =0 .90 5L - 4.76 ;幼贝期H =0 .0 14 +0 .82 4L ;成贝期H =0 .36 7+0 .72 4L。     

改进重铬酸钾法快速测定水样CODcr

研究了用硫酸锰代替贵金属硫酸银作催化剂,在H2SO4-H3PO4介质中,重铬酸钾回流法测定水样中CODcr的新方法.通过实验,确定了最佳测定条件:MnSO4浓度为1×10-1mol.L-1;混酸溶液(H2SO4:H3PO4)=5:1(体积比);回流时间10min.并与标准方法进行了对比,准确度和精密度同标准法相近,大大节约了分析时间.     

有机组分二次离子质谱特征及其裂解机理

二次离子质谱(SIMS)技术近年来被广泛用于分析煤和油气烃源岩有机组分化学成分和结构特征.在介绍SIMS质谱图解析方法的基础上,分析了有机组分二次离子组成特征;在综合研究有机组分SIMS质谱图特征的基础上,应用化学动力学原理对有机组分SIMS质谱裂解机理进行了探讨.结果表明:有机组分以芳香核为基本结构单元,且含有杂环并带有多种含氧基团和支链;有机组分SIMS质谱裂解由其复杂大分子结构中烷基苯不同化学键的解离能大小决定的;有机质结构中α碳和β碳之间的α—p键和支链碳的C—C键解离能最弱,有机化学结构中的α-β键和支链碳的C—C键容易断裂;与苯环相连支链中,(C6 H5) CH2—C2H5结构中的α-β键和支链碳的C—C键容易断裂而形成(C6H5)CH2-(91)、C2H5-(29)和CH3-(15)等碎片离子,这也是有机组分SIMS质谱图中最主要的碎片离子峰.     

应用2H、18O同位素示踪华北平原石家庄包气带土壤水入渗补给及年补给量确定

采集2个不同深度包气带土壤水2H、18O同位素剖面ZK1,ZK2,应用天然稳定同位素2H、18O示踪的方法,研究了华北
山前冲积平原石家庄地区包气带土壤水入渗补给的历史演化特征。结果显示,研究区内,以0.05m 为取样间隔,δ2H、δ18O值可
以明显指示出大气降水及灌溉水入渗补给时间—剖面深度位置的年际对应关系。ZK2的δ2H、δ18O值随着埋深的增大出现周期
性的波动,具有分层现象的岩性差异并不明显,说明ZK2剖面以活塞流的入渗方式补给地下水。在0~3.90m深度,δ2H、δ18O值
显示降水和灌溉水的入渗补给时间为2011年10月至2001年11月。18O峰值位移法计算补给量的结果显示,降水、灌溉水通过包
气带补给地下水的垂向运移速度为38.5~65.0cm/a,年均入渗补给量为131.3~185.3mm。同时,对比2003年及2005-2007
年降水量数据,说明少雨年份农业灌溉用水量的大小对当地地下水的入渗补给量起着关键性作用。      

Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp.NJ298 in low temperature

The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.     

Information for authors

正1 Aims and Scope Advances in Polar Science(APS)is an international,peer-reviewed journal jointly sponsored by the Polar Research Institute of China and the Chinese Arctic and Antarctic Administration.APS is a comprehensive academic journal dedicated to presentation of multi-disciplinary achievements in Arctic and Antarctic expeditions and research.Its primary purpose     

JOURNAL OF OCEAN UNIVERSITY OF CHINA

<正>Copyright Submfnion of a roanusatpt inks:that the work described has not been published before(exoept In the form of an abstrW or as part of a published lecture,review,or thesis);that it is not under consideration for publication elsewhere that its publication has been approved by ail     

Information for Authors

<正>The Journal of Ocean University of China(Oceanic and Coastal Sea Research)is a comprehensive academic quarterly sponsored by the Ocean University of China,published one volume per year.This journal is devoted to the publication of the theoretical and applied research results on oceanography and marine fisheries.The fieldcovered include the physical,chemical,biological,geo-     

Hydraulics and Hydrology in Mountain Areas

<正>The 35th IAHR World Congress was held in Chengdu,China from September 8 to September 132013.The title of the conference was:"The Wise Finds Pleasure in Water:Meandering through Water Science and Engineering".During the congress,1239 papers were presented in 139sessions for oral presentations and 7 sessions for poster presentations.After the Congress,a Committee was appointed to select some of the best papers presented at the Congress for publication,after a     

Sea ice thickness measurement in spring season in Bothnian Bay using an electromagnetic induction instrument

As an important component of the cryosphere,sea ice is very sensitive to the climate change.The study of the sea ice physics needs accurate sea ice thickness.This paper presents an electromagnetic-induction(EM) technique which can be used to measure the sea ice thickness distribution efficiently,and the successful application in Bothnian Bay.Based on the electromagnetic field theory and the electrical properties of sea ice and seawater,EM technique can detect the distance between the instrument and the ice/water interface accurately,than the sea ice thickness is obtained.Contrastive analysis of the apparent conductivity data obtained by EM and the value of drill-hole at same positions allows a construction of a transformable formula of the apparent conductivity to sea ice thickness.The verification of the sea ice thickness calculated by this formula indicates that EM technique is able to get reliable sea ice thickness with average relative error of only 12%.The statistic of all ice thickness profiles shows that the level ice distribution in Bothnian Bay was 0.4-0.6 m.     

Thermohaline structure inhomogeneity associated with polynia at the northern margin of Emery Ice-shelf

Based on the hydrographic data in austral summer during the 22nd Antarctic Expedition of China(2005/2006),some features can be found about the northern margin of Emery ice shelf as follows.The heat content in the surface layer(0-50 m) at the eastern end and the western end of the ice-shelf margin is much higher than that at the middle.The upper mixing-layer depth and the seasonal thermocline depth at the middle of the ice-shelf northern margin are much shallower than those at the both ends.However there is much less difference between the middle and the ends in the bottom layer.The remote sensing photos show that the inhomogeneity in the surface-layer water is closely related to the spatial distribution of the floes and polynia in the area.     

Phylogenetic analysis of cultivable bacteria isolated from Arctic sea-ice

Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter.     

Information for authors

正1 Aims and Scope Advances in Polar Science(APS)is an international,peer-reviewed journal jointly sponsored by the Polar Research Institute of China and the Chinese Arctic and Antarctic Administration.APS is a comprchensive academic journal dedicated to presentation of multi-disciplinary achievements in Arctic and Antarctic expeditions and research.Its primary purpose is to publish achievements in fundamental research,applied