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1.
Biofouling is the undesirable accumulation of microorganisms, plants, algae and animals on submerged structures especially ship hulls. Biofouling also occurs on the surface of living marine organisms. It is also found on membrane systems such as membrane bioreactors and reverse osmosis spiral wound membranes. In the same manner, it is found in cooling water cycles of large industrial equipments and power stations. In the present study, totally 11 isolates were obtained from three ships from Royapuram harbour, Chennai, Tamil Nadu, India. Among the 11 isolates only DR4 showed maximum biofouling activity in the microtiter plate assay with a significant optical density of 0.596. Also an attempt was made to characterize the different biofouling bacterial isolates analyzing their morphological, biochemical and molecular characteristics. The results of the present study based on the above characteristics revealed that the isolate DR4 was similar to Bacillus sp. This study also highlights the need for a safe and natural antifouling agent to control the biofouling bacteria in the marine environment.  相似文献   

2.
The rock mass failure process can be divided into several distinct deformation stages: the compaction stage, elastic stage, stable failure stage, accelerated failure stage, and post-peak stage. Although each stage has been well studied, the relationship among the stages has not been established. Here, we establish two models which are the Strain model Q and Energy density model S by using the renormalization group theory and investigate the mechanical relationship between the volume dilatant point and peak stress point on the rock stress-strain curve. Our models show that the strain ratio (ε f /ε c ) and energy ratio (E f /E c ) at the volume dilatant point and peak stress point are solely functions of the shape parameter m. To verify our models, we further studied the failure process of rock specimens through several uniaxial compression experiments and found that the relationship between ε f /ε c or E f /E c and m shares a notably similar pattern to that from our theoretical model. However, the ε f /ε c and E f /E c values in our experiments are slightly smaller than those predicted by the models. In brief, we demonstrate that our models can be used to predict the failure process of the laboratory-scale hard brittle rock samples.  相似文献   

3.
A technique for IR spectroscopic determination of the total nitrogen content N S in the form of A-and B 1-defects is suggested. It provides for the computer processing and decomposition of IR spectra into constituent bands, calculation of the total absorption band area S N and individual areas S A and S B1 and their normalization with respect to the total area of the diamond intrinsic absorption S 0, with the normalization coefficients K S , K A , and K B1 being calculated. Based on the analysis of the IR spectra of 60 octahedral diamond crystals from the Mir and Yubileinaya pipes (Sakha-Yakutiya), the empirical functions N S = 911.85 K S 0.9919 ppm (R 2 = 0.9859), N A = 1185.6 K A 1.1511 ppm (R 2 = 0.8703), and N B1 = 911.85 K S 0.9919 ? 1185.6 K A 1.1511 ppm have been defined.  相似文献   

4.
Exopolysaccharides (EPS) are polymeric substances composed of sugars and are produced by microbes to form colonies and inhibit the growth of other organisms. In the present study, the bacterial strain Bacillus sp. YP03 was isolated from soil sample using a high-sucrose selective medium, the produced EPS recovered using ethanol precipitation, and lyophilized. In sugar analysis, it was found to be rich in fructose content, amounting to 86.1%, and further displayed appreciable antioxidant, emulsification, and flocculation activities. EPS concentration of 140 µg/ml, pH of 7.5, and the presence of trivalent metal cations augmented its flocculating ability. When applied as a coagulant aid in the treatment of municipal wastewater, up to 47 and 89% reductions of chemical oxygen demand and total suspended solids, respectively, were witnessed. Owing to these encouraging results, Bacillus sp. EPS could be applied as a potentially beneficial flocculant in wastewater treatment, for achieving accentuated TSS and organic load removal during primary sedimentation.  相似文献   

5.
This paper investigates, using the random field theory and Monte Carlo simulation, the effects of random field discretization on failure probability, p f, and failure mechanism of cohesive soil slope stability. The spatial sizes of the discretized elements in random field Δx, Δy in horizontal and vertical directions, respectively, are assigned a series of combinational values in order to model the discretization accuracy. The p f of deterministic critical slip surface (DCSS) and that of the slope system both are analyzed. The numerical simulation results have demonstrated that both the ratios of Δy/λ y (λ y  = scale of fluctuation in vertical direction) and Δx/λ x (λ x  = scale of fluctuation in horizontal direction) contribute in a similar manner to the accuracy of p f of DCSS. The effect of random field discretization on the p f can be negligible if both the ratios of Δx/λ x and Δy/λ y are no greater than 0.1. The normalized discrepancy tends to increase at a linear rate with Δy/λ y when Δx/λ x is larger than 0.1, and vice versa for p f of DCSS. The random field discretization tends to have more considerable influence on the p f of DCSS than on that of the slope system. The variation of p f versus λ x and λ y may exhibit opposite trends for the cases where the limit state functions of slope failure are defined on DCSS and on the slope system as well. Finally, the p f of slope system converges in a more rapid manner to that of DCSS than the failure mechanism does to DCSS as the spatial variability of soil property grows from significant to negligible.  相似文献   

6.
In this study, we are reporting bacterial diversity in crude oil-contaminated soil of Assam, India. Integration of physiological community profiling, culture-dependent and culture-independent (metagenome) approaches, was employed to obtain a complete picture of the total bacterial diversity. Samples collected from 10 sites contaminated with crude oil ranging from 0.22 to 89.36% were analysed, and altogether 160 culturable bacteria were isolated (117 Gram-positive and 43 Gram-negative bacteria). Molecular identification showed the predominance of genera Lysinibacillus, Alcaligenes, Bacillus, Clostridium, Enterobacter and Pseudomonas. Conversely, denaturing gradient gel electrophoresis (DGGE) profiles of 16S rDNA phylotypes showed the predominance of Sphingomonas, Ralstonia, Sphingobium, Massilia, Acinetobacter and Pseudomonas. Both culture-dependent and culture-independent approaches resulted in 11 genera of which Bacillus and Pseudomonas were the key inhabitants creating most favourable bacterial milieu in the presence of crude oil contaminants. Metabolic fingerprints data depicted in PCA plot demonstrated that sites CTF-D-1 and Core-10 were most diverged. It was further confirmed that variations of bacterial species dominance in different sites were due to origin of hydrocarbon contamination. We here claim that the present findings is a first-hand report on combined physiological community profiling, culture-based and culture-independent approaches in assessing total bacterial diversity in crude oil-contaminated soil of Assam.  相似文献   

7.
Previously, similarity of source spectra of Kamchatka earthquakes with respect to the common corner frequency fc1 and the expressed deviations from similarity for the second fc2 and the third fc3 corner frequencies were revealed. The value of fc3 reflects the characteristic size Lis of fault surface; correspondingly, LisvrTis, where vr is the rupture speed and Tis ≈ 1/fc3 is characteristic time. The estimates of fc3 are used for normalizing fc1 and fc2. In this way one obtains dimensionless rupture temporal parametres τ1 and τ2 and can further study the dependence τ21). The growth of a rupture is considered as a process of aggregation of elementary fault spots of the size Lis. The dimensionless width of the random front of aggregation is on the order of τ2. The relationship τ21) approximately follows power law with exponent β. The estimates of β derived from earthquake populations of Kamchatka, USA and Central Asia (β = 0.35–0.6) agree with values expected from the known Eden’s theory of random aggregation growth and from its generalizations.  相似文献   

8.
The improvement in the capabilities of Landsat-8 imagery to retrieve bathymetric information in shallow coastal waters was examined. Landsat-8 images have an additional band named coastal/aerosol, Band 1: 435–451 nm in comparison with former generation of Landsat imagery. The selected Landsat-8 operational land image (OLI) was of Chabahar Bay, located in the southern part of Iran (acquired on February 22, 2014 in calm weather and relatively low turbidity). Accurate and high resolution bathymetric data from the study area, produced by field surveys using a single beam echo-sounder, were selected for calibrating the models and validating the results. Three methods, including traditional linear and ratio transform techniques, as well as a novel proposed integrated method, were used to determine depth values. All possible combinations of the three bands [coastal/aerosol (CB), blue (B), and green (G)] have been considered (11 options) using the traditional linear and ratio transform techniques, together with five model options for the integrated method. The accuracy of each model was assessed by comparing the determined bathymetric information with field measured values. The standard error of the estimates, correlation coefficients (R 2 ) for both calibration and validation points, and root mean square errors (RMSE) were calculated for all cases. When compared with the ratio transform method, the method employing linear transformation with a combination of CB, B, and G bands yielded more accurate results (standard error = 1.712 m, R 2 calibration = 0.594, R 2 validation = 0.551, and RMSE =1.80 m). Adding the CB band to the ratio transform methodology also dramatically increased the accuracy of the estimated depths, whereas this increment was not statistically significant when using the linear transform methodology. The integrated transform method in form of Depth = b 0  + b 1 X CB  + b 2 X B  + b 5 ln(R CB )/ln(R G ) + b 6 ln(R B )/ln(R G ) yielded the highest accuracy (standard error = 1.634 m, R 2 calibration = 0.634, R 2 validation = 0.595, and RMSE = 1.71 m), where R i (i = CB, B, or G) refers to atmospherically corrected reflectance values in the i th band [X i  = ln(R i -R deep water)].  相似文献   

9.
The lipA gene, encoding a solvent-tolerant extracellular lipase from Proteus sp. SW1, was displayed on the cell surface of Escherichia coli by fusing it to an antigen 43 anchoring motif. The display of LipA on the Escherichia coli cell surface was directly confirmed by immunofluorescence microscopy and flow cytometry. After 6 days of incubation in media containing 1 % used cooking oil, an Escherichia coli strain expressing surface displayed lipase was able to degrade 27 % of the oil. The biosurfactant, pseudopyronine B, was purified from culture supernatants of Pseudomonas sp. SL31. Its critical micelle concentration was determined to be 1400 mg/l, and the surfactant was stable within a temperature range from 0 to 120 °C and a pH range of 3–11. Pseudopyronine B-containing crude media extracts efficiently removed up to 51 % of the cadmium from contaminated water. We demonstrated the oil degradation ability of the mixed culture of four bacterial strains, namely the recombinant Escherichia coli expressing cell surface displayed lipase (pKKJlipA), His-tagged lipase (pETlipA), extracellular lipase-producing Proteus sp. SW1, and pseudopyronine B-producing Pseudomonas sp. SL31 by culturing in LB media containing 1 % oil. The consortium degraded 29 % of oil in one day and reached 84 % after 7 days.  相似文献   

10.
The effect of bicarbonate (HCO3?) on the growth and development of plants varies by species. To better understand inorganic carbon and nitrogen assimilation changes of karst-adaptable plants under different HCO3? treatments, we conducted experiments on seedlings and in vitro plantlets of Orychophragmus violaceus (Ov). We found that the vital photosynthesis potential (as measured by net photosynthetic rate, actual photochemical efficiency of photosystem-II, photochemical quenching coefficient, and the instantaneous carbon isotope ratio of 3-phosphoglycerate) was consistent under different HCO3? treatments of Ov. Bicarbonate’s lack of effect on carbon assimilation of Ov may be related to carbonic anhydrase in Ov converting HCO3? to H2O and CO2. In this way, Ov could prevent HCO3? ion toxicity and high pH from harming its growth and development under HCO3? stress. This study also found that high HCO3? concentrations could promote nitrogen assimilation and utilization of Ov through changes in related indexes (foliar nitrogen isotope fractionation ratio, stable nitrogen isotope assimilation ratio, foliar stable nitrogen isotope fractionation, nitrate nitrogen utilization efficiency, and nitrate utilization share) under different HCO3? treatments. Bicarbonate has different effects on photosynthesis and on inorganic nitrogen assimilation of Ov, which may be connected to photosynthesis providing electrons for nitrate/nitrite reduction through the photosynthetic chain.  相似文献   

11.
Absorption of the synchrotron emission of the quasar 3C 345 in the continuum and H(93–95)α and H(78–79)α radio recombination lines is studied. The upper limit for absorption in the H(93–95)α lines is Tal/Tac < 0.7%; absorption in the H(78–79)α lines with antenna temperature Tal = 25 mK, linewidth Δf = 5.3 ± 0.08 MHz, and Tal/Tac ≥ 0.3% has been detected. A correction to the redshift Δz = 0.00135 ± 0.00008 (z = 0.59365) has been determined.  相似文献   

12.
The results of hydrodynamical calculations of radially pulsating helium stars with masses 0.5MM≤0.9M, bolometric luminosities 600L≤5×103L, and effective temperatures 1.5×104 K≤Teff≤3.5×104 K are presented. The pulsation instability of these stars is due to the effects of ionization of iron-group elements in layers with temperatures T~2×105 K. The calculations were carried out using opacities for the relative mass abundances of hydrogen and heavy elements X=0 and Z=0.01, 0.015, and 0.02. Approximate formulas for the pulsation constant Q over the entire range of pulsation instability of the hot helium stars in terms of the mass M, radius R, effective temperature Teff, and heavy-element abundance Z are derived. The instability of BX Cir to radial pulsations with the observed period Π=0.1066 d occurs only for a mass M≥0.55M, effective temperature Teff≥23000 K, and heavy-element abundance Z≥0.015. The allowed mass of BX Cir is in the range 0.55MM≤0.8M, which corresponds to luminosities 800LM≤1400L and mean radii 1.7R?R?2.1R.  相似文献   

13.
A high-pressure single-crystal X-ray diffraction study has been carried out on a P21/c natural Mg-rich pigeonite sample with composition ca. Wo6En76Fs18 using a diamond anvil-cell. The unit-cell parameters were determined at 14 different pressures to 7.14 GPa. The sudden disappearance of the b-type reflections (h + k = odd) and a strong discontinuity (about 2.8%) in the unit-cell volume indicated a first-order P21/cC2/c phase transition between 4.66 and 4.88 GPa. The P(V) data of the P21/c phase were fitted to 4.66 GPa by a third-order Birch–Murnaghan equation of state (BM3 EoS), whereas the limited number of experimental data collected within the C2/c phase between 4.88 and 7.14 GPa were fitted using the same equation of state but with K′ constrained to the value obtained for the P21/c fitting. The equation of state coefficients are V 0 = 424.66(6) Å3, K T0 = 104(2) GPa and K′ = 8(1) for the P21/c phase, and V 0 = 423.6(1) Å3, K T0 = 112.4(8) GPa, and K′ fixed to 8(1) for the C2/c phase. The axial moduli for a, b, and c for the P21/c phase were obtained using also a BM3-EoS, while for the C2/c phase only a linear calculation could be performed, and therefore the same approach was applied for comparison also to the P21/c phase. In general the C2/c phase exhibits axial compressibilities (β c > β a >> β b) lower than those of the P21/c phase (β b > β c ≈ β a; similar to those found in previous studies in clinopyroxenes and orthopyroxenes). The lower compressibility of the C2/c phase compared with that of the P21/c could be ascribed to the greater stiffness along the b direction. A previously published relationship between P c and M2 average cation radius (i.r.) has been updated using all the literature data on P21/c clinopyroxene containing large cations at M2 site and our new data. The following weighted regression was obtained: P c (GPa) = 26(4) ? 28(5) ×  i.r (Å), R 2 = 0.97. This improved equation can be used to predict the critical pressure of natural P21/c clinopyroxene samples just knowing the composition at M2 site.  相似文献   

14.
The strong adaptability of Broussonetia papyrifera (L.) Vent. to low phosphorus (P) conditions can be attributed to the large amount of root-exuded organic acids and the high efficiency of P extraction. However, microelement contents are influenced by low-P stress, and their effects on the photosynthetic capability of B. papyrifera remain unknown. In this study, we investigated the effects of low-P treatment on net photosynthetic rate (P N); chlorophyll a fluorescence (ChlF) characteristics; and Fe, Mn, Cu, and Zn contents of B. papyrifera and Morus alba L. seedlings. Results show that B. papyrifera exhibited better photosynthetic capability under moderate P deficiency (0.125, 0.063, and 0.031 mmol/L P treatments), whereas the photosynthetic capability of M. alba decreased under moderate and severe P deficiency (0.016 and 0 mmol/L P treatments). Under moderate P deficiency, the decrease in Cu and Zn contents in B. papyrifera was lower than that in M. alba. Under severe P deficiency, a considerable decrease of photosynthetic capability in B. papyrifera and M. alba was associated with low Cu and Zn contents. The P N of the two Moraceae species exhibited a better correlation with Cu and Zn contents than with Fe or Mn content. P deficiency could not only decrease cyclic photophorylation and photosynthetic efficiency, but could also affect the stability of thylakoid membrane structure and electron transport efficiency by influencing the contents of Cu or Zn, thereby affecting photosynthesis.  相似文献   

15.
The Al-rich region of the CaO-MgO-Al2O3-SiO2 system was experimentally studied at pressures of 1.0–2.8 GPa and temperatures of 1300–1535°C. The slopes of the lines of the monovariant reactions An + Sp = Cpx + Cor + (Ga) and L = Cpx + Ga + Cor + Sp and the compositions of the phases involved in these reactions are determined. The results are utilized in the topological analysis of the aluminous region of the CaO-MgO-Al2O3-SiO2 system. On this basis, the principal structure of the phase diagram is analyzed, and a phase diagram is constructed for the junction region of the quaternary system and the CaO-Al2O3-SiO2 ternary system. A continuous series of the monovariant eutectic: L = Cpx + Opx + Fo + An, L = Cpx + Opx + An + Sp, L = Cpx + (Ga) + An + Sp, L = Cpx + Cor + (Ga) + An, L = An + Ga + Cpx + Ky and L = Ga + Cpx + Ky + Qz is examined within the pressure range from atmospheric to 3.0 GPa and higher. Analogous “telescoped” eutectic series are of fundamental character for interpreting the evolution of magmatic melts. A physicochemical model is suggested for the evolution of magmatic melts that produce rocks of the calc-alkaline series, with this model underlain by the fact that a change in the composition of magmatic melt at a pressure decrease should correspond to the minimum melting temperatures, i.e., to melts in the fundamental series of eutectic reactions. The comparison of our physicochemical model and rocks of the calc-alkaline series shows that the compositions of rocks of the calc-alkaline series are close to the compositions determined for the eutectic equilibria, and the mineralogical composition of xenoliths and megacrysts in volcanic and dike varieties of the rocks are similar to the subsolidus phases of the established fundamental eutectic system.  相似文献   

16.
In the present study, four different heuristic techniques viz. multi-layer perceptron (MLP), radial basis function (RBF), self-organizing maps (SOM), and co-active neuro-fuzzy inference system (CANFIS) with hyperbolic tangent and sigmoid transfer functions and two regression-based techniques, i.e., multiple linear regression (MLR) and sediment-rating curve (SRC), were used for suspended sediment modeling. Gamma test (GT), correlation function (CF), M test, and trail–error procedure were applied for estimation of appropriate input variables as well as training data length. The results of the GT and CF suggested the five input variables (Qt, Qt?1, Qt?2, St?1, and St?2, where Qt?1 and St?1 indicate the discharge and sediment values of one previous day) as the best combination. The optimal training data length (75% of total data) was estimated by M test and trail–error procedure for development of the applied models. The MLP with sigmoid transfer function (M-2) performed better than the all other models. The results of sensitivity analysis indicated that the present-day discharge (Qt), 1-day lag discharge (Qt?1) and 1-day lag suspended sediment (St?1) are the most influenced parameters in modeling current day suspended sediment (St).  相似文献   

17.
Pyroxenes of general stoichiometry Mg(Ge x Si1?x )O3 were encountered in attempts to synthesise Ge-substituted talcs at 0.2 GPa, 650–700 °C. Orthopyroxenes (Pbca) of compositions x = 0.21, 0.30, and 0.34 were identified, and also a P21/c clinopyroxene of composition x = 0.63, and C2/c clinopyroxenes of compositions x = 0.91 and 1. End-member clinoenstatite MgSiO3-P21/c synthesised at 16 GPa, 1300 °C and transformed from C2/c was also included in the study. Crystal structure refinements using single-crystal XRD data showed that unit-cell parameters vary linearly with Si–Ge for the Pbca and P21/c pyroxenes, both of which have two symmetrically non-equivalent tetrahedral chains. Refinement of Si–Ge occupancies at tetrahedral sites showed that the two chains of all primitive pyroxenes have very different compositions, with XGe(TB) ? XGe(TA). This difference arises from the greater flexibility of the B-chain to rotate in response to tetrahedral expansion due to increasing Ge content. The TA-M2 shared polyhedral edge imposes significant constraints on the flexibility of the A-chain, which can accommodate much less Ge than the B-chain. Linear trends of cell parameters, site occupancies, and structural parameters for the primitive pyroxenes, when extrapolated to published data for MgGeO3Pbca, extend across the entire Si–Ge join.  相似文献   

18.
The high-pressure behavior of a vanadinite (Pb10(VO4)6Cl2, a = b = 10.3254(5), = 7.3450(4) Å, space group P63/m), a natural microporous mineral, has been investigated using in-situ HP-synchrotron X-ray powder diffraction up to 7.67 GPa with a diamond anvil cell under hydrostatic conditions. No phase transition has been observed within the pressure range investigated. Axial and volume isothermal Equations of State (EoS) of vanadinite were determined. Fitting the PV data with a third-order Birch-Murnaghan (BM) EoS, using the data weighted by the uncertainties in P and V, we obtained: V 0 = 681(1) Å3, K 0 = 41(5) GPa, and K′ = 12.5(2.5). The evolution of the lattice constants with P shows a strong anisotropic compression pattern. The axial bulk moduli were calculated with a third-order “linearized” BM-EoS. The EoS parameters are: a 0 = 10.3302(2) Å, K 0(a) = 35(2) GPa and K′(a) = 10(1) for the a-axis; c 0 = 7.3520(3) Å, K 0(c) = 98(4) GPa, and K′(c) = 9(2) for the c-axis (K 0(a):K 0(c) = 1:2.80). Axial and volume Eulerian-finite strain (fe) at different normalized stress (Fe) were calculated. The weighted linear regression through the data points yields the following intercept values: Fe a (0) = 35(2) GPa for the a-axis, Fe c (0) = 98(4) GPa for the c-axis and Fe V (0) = 45(2) GPa for the unit-cell volume. The slope of the regression lines gives rise to K′ values of 10(1) for the a-axis, 9(2) for the c-axis and 11(1) for the unit cell-volume. A comparison between the HP-elastic response of vanadinite and the iso-structural apatite is carried out. The possible reasons of the elastic anisotropy are discussed.  相似文献   

19.
This paper presents the first boundary equations describing the relationship between earthquake parameters (magnitude M S and macroseismic intensity I P at the observation point on the MSK-64 scale) and clastic dikes (having maximal thickness m cd , visible height h cd , and the index of manifestation intensity of dikes in the cross section I cd ). As was expected, the maximal size of dikes grows with an increase in the earthquake magnitude and macroseismic intensity. Analysis of the dependences showed that it is better to use all three parameters for estimation of the minimal threshold M S or I P from clastic dikes, and, in the absence of data on seismogenic rupture, the maximal calculated value should be used. Some limitations in application and the advantages of the equations obtained are discussed with respect to characterizing earthquakes of the pre-instrumental period.  相似文献   

20.
A mechanism for the separation of chemical elements and isotopes in the atmospheres of chemically peculiar (CP) stars due to light-induced drift (LID) of ions is discussed. The efficiency of separation due to LID is proportional to the relative difference of the transport frequencies for collisions of ions of heavy elements located in the excited state (collision frequency ν e ) and ground state (collision frequency ν g ) with neutral buffer particles (hydrogen and helium), (ν e ? ν g )/ν g . The known interaction potentials are used to numerically compute the relative difference (ν e H ? ν g H )/νg H for collisions between the ions Be+, Mg+, Ca+, Sr+, Cd+, Ba+, Al+, and C+ and hydrogen atoms. These computations show that, at the temperatures characteristic of the atmospheres of CP stars, T = 7000?20 000 K, values of |ν e H g H |/ν g H ≈ 0.1?0.4 are obtained. With such relative differences in the transport collision frequencies, the LID rate of ions in the atmospheres of coolCP stars (T < 10000 K) can reach ~0.1 cm/s,which exceeds the drift rate due to light pressure by an order of magnitude. This means that, under these conditions, the separation of chemical elements under the action of LID of ions could be an order of magnitude more efficient than separation due to light pressure. Roughly the same manifestations of LID and light pressure are also expected in the atmospheres of hotter stars (20 000 > T > 10 000 K). LID of heavy ions is manifest only weakly in very hot stars (T > 20 000 K).  相似文献   

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