Hypersalinity During Regional Drought Drives Mass Mortality of the Seagrass <Emphasis Type="Italic">Syringodium filiforme</Emphasis> in a Subtropical Lagoon

Seagrasses are sensitive to local environmental conditions such as salinity, the underwater light environment, and nutrient availability. To characterize seagrass coverage and condition, as well as to relate changes in community structure to local environmental and hydrologic conditions, we monitored seagrass communities in the Upper Laguna Madre (ULM), Texas annually from 2011 to 2015. In 2011 and 2012, the lagoon was dominated primarily by Halodule wrightii, with mixed meadows of H. wrightii and Syringodium filiforme located in the northwest of our study area. By 2013, the expansive S. filiforme meadows had disappeared and the species was restricted to the northernmost reaches of the lagoon. The S. filiforme mortality occurred following an extended period of extremely high salinity (salinities 50–70) during a regional drought. Continuous measurements of underwater photosynthetically active radiation and stable carbon isotopic signatures of seagrass blade tissues did not suggest light limitation, and H. wrightii N/P molar ratios near 30:1 were not indicative of nutrient limitation. Based on the absence of strong evidence for light or nutrient limitation, along with the known tolerance of H. wrightii for higher salinities, we conclude that hypersalinity driven by regional drought was likely the major driver behind the observed S. filiforme mortality. With a substantial portion of the global seagrass distribution threatened by drought in the next 50 years, the increased frequency of hypersaline conditions is likely to exacerbate stress in seagrass systems already vulnerable to the effects of rising water temperatures, eutrophication, and sea level rise.     

Isotopic and Elemental Composition of Marine Macrophytes as Biotracers of Nutrient Recycling Within a Coastal Lagoon in Baja California,Mexico

Nutrient sources of San Quintin Bay, a coastal lagoon affected by coastal upwelling off Baja California (Mexico), were traced using generalized additive (mixed) models (GAMM) to the stable nitrogen isotopic composition, C:N and N content of two co-occurring macrophytes (the macroalgae Ulva spp. and the seagrass Zostera marina). The geochemical tracers followed a spatial trend that partly responded to the long-term nutrient gradient from the ocean towards the interior of the bay. N content in Z. marina and Ulva spp. decreased linearly (while C:N increased) towards the middle section of the bay to concentration levels that indicate potential N limitation for growth. Concurrently midway into the bay (6–9 km), the δ¹⁵N of both macrophytes showed a gradual enrichment in ¹⁵N reflecting progressive denitrification. The spatial pattern of δ¹⁵N and the decrease in C:N of the macrophytes towards the innermost section of the bay indicated an additional nonoceanic source of dissolved nitrogen in this zone. The similarity of the δ¹⁵N pattern of Z. marina and Ulva spp. implies that their δ¹⁵N composition is mainly controlled by the availability of N, in spite of the physiological differences between taxa. A better fit of GAMM to N content and C:N was obtained for Z. marina than for Ulva spp. indicating that the former delineate more steadily and smoothly the influence of upwelling along the spatial gradient. Nonetheless, Ulva spp. may be analyzed in combination with Z. marina to characterize the environmental conditions at the time of sampling.     

Present environmental status of Al-Kharrar Lagoon,central of the eastern Red Sea coast,Saudi Arabia

Al-Kharrar Lagoon is a fossil back-reef basin with hypersaline waters, situated 10 km northwest of Rabigh city, central of the eastern Red Sea coast, Saudi Arabia. About 130 stations were selected for measurements of the lagoon’s water temperature, salinity, pH, dissolved oxygen, and water depths during March 2014. The common macro-algae, flora, and fauna were also sampled and identified. The present study aims to investigate the prevailing environmental parameters and their impact on the macro-fauna/flora of the lagoon. The average water depth of the lagoon was around 5 m and reached maximum values of 8 and 16 at the lagoon centre and inlet, respectively. The results showed that the lagoon’s surface water temperature and salinity have mean values of 25 °C and 40‰, but with extreme values of 30 °C and 45‰ that occurred only at the enclosed intertidal areas, respectively. Their dissolved oxygen (DO) and pH were 6.5 mg/l and 8.3, respectively and the latter showing the highest values up to 8.5 in the intertidal areas dominated by the green cyanobacteria. These physicochemical conditions make the lagoon as a favorite place for the mangrove Avicennia marina, macro-algae, seagrasses (Halophila stipulacea and Cymodocea rotundata), and algal mats (Cyanobacteria) which dominate the intertidal and supratidal areas of the lagoon, tolerating extremely high-salinity and high-temperature conditions. On the other hand, corals were observed alive at the southern part of the lagoon, immediately south of the Al-Ultah Islet. Vertical profiles of temperature, salinity, and density in the lagoon’s water indicated that the water column consists of two layers throughout the year.     

Functional,Phylogenetic and Host-Geographic Signatures of <Emphasis Type="Italic">Labyrinthula</Emphasis> spp. Provide for Putative Species Delimitation and a Global-Scale View of Seagrass Wasting Disease

Seagrass meadows form ecologically and economically valuable coastal habitat on every continental margin except the Antarctic, but their areal extent is declining by approximately 2–5 % per year. Seagrass wasting disease is a contributing factor in these declines, with the protist Labyrinthula identified as the etiologic agent. To help elucidate the role of Labyrinthula spp. in global seagrass declines, we surveyed roughly one fourth of all seagrass species to identify Labyrinthula diversity at the strain and/or species level, combining results from culturing methods and two common nuclear DNA markers: the ITS and 18S regions of the ribosomal RNA gene complex. After assaying a subset of the resulting isolates (of which 170 were newly sequenced), we produced a cladogenic context for putative seagrass-pathogenic versus non-pathogenic Labyrinthula while also defining host and geographic ranges. Assays also suggest that pathogenicity is consistently high (when present; and, even when comparing susceptibility of US East- versus West Coast Zostera marina hosts) while virulence is variable, that some isolate-host combinations have the potential for host cross-infection, and that several modes of transmission can be effective. Taken together, these data provide additional means for delimiting putative species of Labyrinthula, suggesting at least five seagrass-pathogenic and perhaps ten or more non-pathogenic marine “species”, yielding a working definition for ecologists and epidemiologists attempting to reconcile the sundry data related to seagrass wasting disease.     

Prevalence of pathogenic microorganisms and their correlation with the abundance of indicator organisms in riverbed sediments

The present study investigated the prevalence of pathogenic organisms (Salmonella spp, Vibrio cholerae, and Shigella spp) and their correlation to the abundance of faecal indicator organisms in water and riverbed sediments in the Apies River, South Africa. In all, 558 water and sediment samples were collected from 10 sites in the river (May 2013–February 2014) and analysed through culture and molecular (real-time PCR) techniques. Concentrations of faecal indicator organisms in sediments reached 1.39 × 10⁵ (±standard deviation) CFU/100 mL. All three pathogens were detected in water and sediments. Pathogens were mostly detected in sediments at sites influenced either by wastewater treatment works or by informal settlements. During the wet and dry seasons (water column), a strong positive correlation was observed between E. coli and all pathogens; C. perfringens only correlated with V. cholerae. Within sediments, strong positive correlations were only observed between E. coli and Salmonella spp, E. coli and V. cholerae (dry season); E. coli and V. cholerae and E. coli and Shigella spp (wet season). No correlation was observed between sediments C. perfringens counts and all the pathogens. Thus, sediments of the Apies River harbour pathogenic organisms. Correlation between E. coli and pathogenic organisms in the sediments suggests that E. coli could also be an indicator of pathogens’ presence. However, the lack of a correlation between E. coli and some pathogens in sediments and between C. perfringens and all the pathogens highlights the need to investigate for more indicators of pathogens’ presence in this complex matrix.     

Early Eocene planktonic and benthic foraminifera from the Khangiran formation (northeast of Iran)

The Kopet-Dagh sedimentary basin is located in northeast of Iran and southeast of Turkmenistan in the Middle East. The Khangiran formation represents the last marine deposition in the Kopet-Dagh sedimentary basin. The early Eocene planktonic and benthic foraminifera from the Khangiran formation which belongs to the lower 376 m thickness of this formation are identified and biostratigraphically evaluated. Due to rarity of Morozovella species, planktonic foraminiferal zonation was difficult to determine in this formation. The determination of upper part of the late Paleocene for the lower 124 m of this formation is according to the lowest occurrence of Acarinina sibaiyaensis species. From E₅ to near middle of E₇ biozone, increasing trend of Acarinina frequency and a peak in Morozovella species and decreasing trend of frequency of Subbotina and Pseudohastigerina species indicate the warm and oligotrophic condition of the seawater during sedimentation of the studied interval. Toward the Ypresian-Lutetian boundary, the increasing trend of Pseudohastigerina and Subbotina species and decreasing in frequency of Acarinina species suggest the low-oxygen level, eutrophic and intermediate condition of the seawater. In this formation, the high abundance of the epifaunal taxa such as Anomalinoides spp., Cibicidoides spp., Gyroidinoides spp., and Lenticulina spp. from the base (late Paleocene sediments) up to E₆ biozone reflects oligotrophic and oxic shallow water conditions. The occurrence of several peaks in abundance of Bulimina and Uvigerina species at the Ypresian/Lutetian boundary suggests eutrophic condition. These paleoecological conditions could be correlated with fluctuations in the numbers of the planktonic foraminifera.     

Permian Calcareous algae from the Khachik Formation at the Ali Bashi Mountains,NW of Iran

Micropaleontological investigations on Permian successions in the NW of Iran based on algae led to the determination of taxa belonging to some families such as Ungdarellaceae (Ungdarella uralica), Gymnocodiaceae (Gymnocodium bellerophontis and Permocalculus sp.), Dasycladales (Mizzia cf. M. yabei), and some microproblematics (Vermiporella nipponica and Pseudovermiporella sodalica). These investigations are mainly focused on the Khachik Formation at the Ali Bashi Mountains. According to the algal community, a lagoon environment is offered for the studied successions.     

Principal component analysis for interaction of nitrifiers and wastewater environments at a full-scale activated sludge plant

This study investigated the relationship between wastewater environments and the nitrifiers at a full-scale plant using principal component analysis. Ammonia-oxidizing bacteria and nitrite-oxidizing bacteria were detected by florescent in situ hybridization, polymerase chain reaction, phylogenetic analysis, real-time quantitative polymerase chain reaction. Pyrosequencing was also used in profiling the ammonia monooxygenase locus of ammonia-oxidizing bacteria community. It was found that the dominant ammonia-oxidizing bacteria sequences were related to uncultured ammonia-oxidizing bacterium, uncultured Nitrosomonadaceae bacterium, Nitrosomonas sp., and uncultured bacterium. In addition, Nitrobacter clones were related to uncultured alpha proteobacterium, uncultured bacterium, uncultured Nitrobacter sp., and uncultured Bradyrhizobium sp., whereas Nitrospira clones were similar to uncultured bacterium, Candidatus Nitrospira defluvii, uncultured Nitrospira sp., and uncultured Nitrospirae bacterium. The ammonia-oxidizing bacteria and nitrite-oxidizing bacteria ranged 2.83 × 10⁸–1.33 × 10¹⁰ and 1.25 × 10¹⁰–1.13 × 10¹¹ copies L^?1, respectively, equivalent to nitrite-oxidizing bacteria: ammonia-oxidizing bacteria ratio of 10:1. The first three parts of the principal components analysis accounted for 76.8% of the explained variance. The first principal component (44.4%) designated that ammonia-oxidizing bacteria and Nitrospira were mainly influenced by seasonal variations, followed by chemical oxygen demand concentration and nitrogen species (i.e., ammonia, nitrite, and nitrate). The second principal component (19.1%) showed no information about the nitrifiers’ interaction with environmental factors, whereas Nitrobacter demonstrated a high correlation with ammonia on the third principal component (13.3%). These results revealed that the species of Nitrobacter were less influenced by environmental conditions than ammonia-oxidizing bacteria and Nitrospira spp.     

The Bloom-Forming Macroalgae, <Emphasis Type="Italic">Ulva</Emphasis>, Outcompetes the Seagrass, <Emphasis Type="Italic">Zostera marina</Emphasis>, Under High CO<Subscript>2</Subscript> Conditions

While multiple species of macroalgae and seagrass can benefit from elevated CO₂ concentrations, competition between such organisms may influence their ultimate responses. This study reports on experiments performed with a Northwest Atlantic species of the macroalgae, Ulva, and the seagrass, Zostera marina, grown under ambient and elevated levels of pCO₂, and subjected to competition with each other. When grown individually, elevated pCO₂ significantly increased growth rates and productivity of Ulva and Zostera, respectively, beyond control treatments (by threefold and 27%, respectively). For both primary producers, significant declines in tissue δ¹³C signatures suggested that increased growth and productivity were associated with a shift from use of HCO₃^? toward CO₂ use. When grown under higher pCO₂, Zostera experienced significant increases in leaf and rhizome carbon content as well as significant increases in leaf carbon-to-nitrogen ratios, while sediments within which high CO₂ Zostera were grown had a significantly higher organic carbon content. When grown in the presence of Ulva; however, above- and below-ground productivity and tissue nitrogen content of Zostera were significantly lower, revealing an antagonistic interaction between elevated CO₂ and the presence of Ulva. The presence of Zostera had no significant effect on the growth of Ulva. Collectively, this study demonstrates that while Ulva and Zostera can each individually benefit from elevated pCO₂ levels, the ability of Ulva to grow more rapidly and inhibit seagrass productivity under elevated pCO₂, coupled with accumulation of organic C in sediments, may offset the potential benefits for Zostera within high CO₂ environments.     

Palynology of Triassic–Jurassic boundary sections in northern Switzerland

A first palynostratigraphic scheme of Upper Triassic deposits in northern Switzerland was established based on spore-pollen associations and dinoflagellate cyst records from the upper part of the Upper Triassic Klettgau Formation and the lower part of the Lower Jurassic Staffelegg Formation. Drill cores from the Adlerberg region (Basel Tabular Jura) and from Weiach (northern part of Canton Zurich) as well as from an outcrop at the Chilchzimmersattel (Basel Folded Jura) were studied and five informal palynological associations are distinguished. These palynological associations correlate with palynological association of the Central European Epicontinental Basin and the Tethyan realm and provide a stratigraphic framework for the uppermost Triassic sediments in northern Switzerland. Throughout the uppermost Triassic to Jurassic palynological succession a remarkable prominence of Classopollis spp. is observed. Besides Classopollis spp. the three Rhaetian palynological associations A to C from the Upper Triassic Belchen Member include typical Rhaetian spore-pollen and dinoflagellate taxa (e.g., Rhaetipollis germanicus, Geopollis zwolinskae, Rhaetogonyaulax rhaetica, and Dapcodinium priscum). Association B differs from association A in a higher relative abundance of the sporomorph taxa Perinopollenites spp. and the consistent occurrence of Granuloperculatipollis rudis and Ricciisporites tuberculatus. Spore diversity is highest in the late Rhaetian palynological association C and includes Polypodiisporites polymicroforatus. A Rhaetian age for the Belchen Member is confirmed by palynological associations A–C, but there is no record of the latest Rhaetian and the earliest Jurassic. In contrast to the Rhaetian palynological associations the Early Jurassic associations W and D include Pinuspollenites spp., Trachysporites fuscus (in association W), and Ischyosporites variegatus. In the view of the end-Triassic mass extinction and contemporaneous environmental changes the described palynofloral succession represents the pre-extinction phase (associations A and B) including a distinct transgression, the extinction phase (association C) associated with a regression, and the post-extinction phase (association W).     

Seasonal Factors Influencing Copepod Abundance in the Maryland Coastal Bays

Maryland Coastal Bays differ in hydrography from river-dominated estuaries because of limited freshwater inflow from tributary creeks and more marine influence. Consequently, the copepod community structure may be different from that of the coastal ocean and river-dominated estuaries in the mid-Atlantic region. A 2-year study was conducted to describe copepod species composition and seasonal patterns in abundance and factors influencing the community structure. Seven copepod genera, Acartia, Centropages, Pseudodiaptomus, Parvocalanus, Eurytemora, Oithona, and Temora, in addition to harpacticoids were found. The copepod community was dominated by Acartia spp. (64%), followed by Centropages spp. (30%), unlike in river-dominated estuaries in the region where the copepod community is usually dominated by Acartia spp. followed by Eurytemora affinis. Acartia tonsa was the most abundant in summer and fall whereas Centropages spp., Temora sp., Oithona similis, E. affinis, and harpacticoids were most abundant in winter and early spring. Parvocalanus crassirostris and Pseudodiaptomus pelagicus were present in fall and winter but at relatively low densities. The highest mean density of copepods occurred in winter 2012 (36,437 m^?3) and the lowest in spring 2013 (347 m^?3). Low densities occurred through early summer (614 m^?3) coinciding with peak spawning by bay anchovy (Anchoa mitchilli). Bottom-up control via low phytoplankton biomass coupled with top-down control by ctenophores (Mnemiopsis sp.), mysids (Neomysis americana), and bay anchovy was probably responsible for the low copepod densities in spring and early summer. Temperature and salinity were also important factors that influenced the seasonal patterns of copepod species occurrence. The observed seasonal differences in the abundance of copepods have important implications for planktivorous fishes as they may experience lower growth rates and survival due to food limitation in spring/early summer when copepod densities are relatively low than in late summer/fall when copepod abundance is higher.     

Distribution and abundance of hydrobiid snails in a mixed estuary and a coastal lagoon,Argentina

This study examined the environmental factors influencing the distribution and abundance of hydrobiid snails in two estuaries on the northeastern coast of Argentina in a coastal lagoon (Mar Chiquita, 37°40′S, 57°20′W) and a partially mixed estuary (Quequén Grande, 38°30′S, 58°45′W). Five intertidal study sites in each estuary represented a gradient in environmental conditions. Variations in the main environmental factors and in the abundance of hydrobiids were assessed both spatially and seasonally. The three species wereHeleobia australis, Heleobia conexa, andHeleobia parchappii, and they were primarily distributed across a salinity gradient. This pattern was clearly recognizable in the partially mixed estuary, where the abundance ofH. australis decreased as salinity decreased, and the abundance ofH. conexa gradually increased towards the inner reaches of the estuary.H. parchappii was restricted to areas far away from the influence of the tide. Slight differences in the distribution patterns of these species between Quequén Grande and Mar Chiquita were refated to the different dynamics of environmental factors in each estuary.     

Spatial and Temporal Patterns in <Emphasis Type="Italic">Thalassia testudinum</Emphasis> Leaf Tissue Nutrients at the Chandeleur Islands,Louisiana, USA

Seagrasses are submerged marine plants that are anchored to the substrate and are therefore limited to assimilating nutrients from the surrounding water column or sediment, or by translocating nutrients from adjacent shoots through the belowground rhizome. As a result, seagrasses have been used as reliable ecosystem indicators of surrounding nutrient conditions. The Chandeleur Islands are a chain of barrier islands in the northern Gulf of Mexico that support the only marine seagrass beds in Louisiana, USA, and are the sole location of the seagrass Thalassia testudinum across nearly 1000 km of the coastline from west Florida to central Texas. Over the past 150 years, the land area of the Chandeleur Islands has decreased by over half, resulting in a decline of seagrass cover. The goals of this study were to characterize the status of a climax seagrass species at the Chandeleur Islands, T. testudinum, in terms of leaf nutrient (nitrogen [N] and phosphorus [P]) changes over time, from 1998 to 2015, and to assess potential drivers of leaf nutrient content. Thalassia testudinum leaf nutrients displayed considerable interannual variability in N and P content and molar ratios, which broadly mimicked patterns in annual average dissolved nutrient concentrations in the lower Mississippi River. Hydrological modeling demonstrated the potential for multiple scenarios that would deliver Mississippi River water, and thus nutrients, to T. testudinum at the Chandeleur Islands. Although coastal eutrophication is generally accepted as the proximate cause for seagrass loss globally, there is little evidence that nutrient input from the Mississippi River has driven the dramatic declines observed in seagrasses at the Chandeleur Islands. Rather, seagrass cover along the Chandeleur Islands appears to be strongly influenced by island geomorphological processes. Although variable over time, the often elevated nutrient levels of the climax seagrass species, T. testudinum, which are potentially driven by river-derived nutrient inputs, raises an important consideration of the potential loss of the ecosystem functions and services associated with these declining seagrass meadows.     

Facies analysis of Pleistocene limestones from Neil West Coast Formation,Neil Island,Ritchie’s archipelago of South Andaman,India

Petrographic thin section analysis of the samples collected from the type section of Neil West Coast Formation, situated in the west coast of Neil Island yielded moderately preserved coralline red algae, benthic and planktic foraminifers, coral fragments, echinoid spines and gastropod shells. The coralline red algae are represented by both non-geniculate and geniculate forms. The non-geniculate forms belong to melobesids, lithophylloids and mastophoroides. The geniculate forms are represented by species of Amphiroa, Corallina, and Jania. However, the diversity and abundance of coralline algal forms are less in comparison to the benthic foraminifers those are represented by Amphistegina, Neorotalia, Ammonia, Elphidium, Operculina, Assilina, Amphisorus and texularids. Planktic foraminifers like Globigerinoides and other biogenic components viz., gastropod shells, echinoid spines and coral fragments are also common. A foraminiferal-algal grainstone facies has been recognized as observed in the field as well as in thin section analysis. The overall assemblage of the biogenic components and facies analysis indicate intertidal to near shore environment of deposition with high energy condition and increased hydrodynamic activity.     

New record of coralline algae from the Holocene sediments of Agatti Island,Lakshadweep, India

The present paper examines the coralline algal assemblage recovered from the Holocene sediments of Agatti Island, Lakshadweep, India. The assemblage comprises nine species of coralline algae which include Amphiroa fragilisma, Lithophyllum nitorum, Lithophyllum incrustans, Lithoporella melobesioids, Spongites sp., Porolithon craspedium, Aethesolithon problematicum, Clathromorphum parcum, Melobesoideae gen. et spec. indet. Clathromorphum parcumis an endophytic coralline alga reported for the first time from the Indian subcontinent. Out of these, seven species belong to the family Corallinaceae and the two represent the family Hapalidiaceae of the class Rhodophyceae. Coralline algal association of this area is dominated by Lithophylloideae, Melobesioideae and Mastophoroideae which include encrusting to fragmented growth forms. The associated branching corals are well developed and adapted to shallow, warm water, low turbid, protected lagoonal environment.     

Investigation into the change of the porosity parameter during transition from laboratory to reservoir conditions

The purpose of the investigation is to reveal the dependences of P _p = f(C _p) on reservoir conditions and the lithological composition of rocks. The samples were studied using a set of lithological-petrographic investigations. To obtain the dependences on thermobaric conditions, 90 samples of different porosities (C _p) of 15, 20, and 25% were collected. As a result, the general pattern of the change in the rock resistivity during the transition from atmospheric conditions of measuring to reservoir conditions was established. Dependences of porosity parameter P _p on porosity coefficient C _p were obtained for three values of formation water salinity and three reservoir conditions. The measurement errors of the porosity parameter P _p were calculated using dependences obtained under atmospheric conditions.     

Response of Mollusk Assemblages to Environmental Conditions: A Case Study from the Burullus Lagoon,Egypt

The identified mollusks from the Burullus lagoon of Egypt (Eastern Mediterranean) are represented by four bivalve species and four gastropod species. Sources of heavy metals pollution in the Burullus lagoon include phosphate fertilizers, sewage and oil spills from fishing boats.The Cerastoderma glaucum species has a wide distribution in the central part of the studied area with satisfying criteria required for good bio-monitors of pollution. High concentrations of heavy metals are found in water, at the eastern side of the lagoon, while low concentrations were detected in the central and western sides of the lagoon. Pirenella conica constitutes the total gastropods assemblages, reflecting this species tolerance to the high concentration of heavy metals. Meanwhile, the total pelecypods in bottom lagoon sediments are completely absent in the eastern parts of the lagoon, which may be due to the sensitivity of pelecypods to the accumulation of heavy metals.     

Stable carbon and oxygen isotope study on benthic foraminifera: Implication for microhabitat preferences and interspecies correlation

Stable isotopes of benthic foraminifera have widely been applied in micropalaeontological research to understand vital effects in foraminifera. Isotopic fractionations are mainly controlled by ontogeny, bottom/pore water chemistry, habitat preference, kinetic effect and respiration. Discontinuous abundance of a species for isotopic analysis has forced us to select multiple species from down-core samples. Thus standardisation factors are required to convert isotopic values of one species with respect to other species. The present study is pursued on isotopic values of different pairs of benthic foraminifera from the Krishna–Godavari basin and Peru offshore to understand habitat-wise isotopic variation and estimation of isotopic correction factors for the paired species (Cibicides wuellerstorfi–Bulimina marginata, Ammonia spp.–Loxostomum amygdalaeformis and Bolivina seminuda–Nonionella auris). Infaunal species (B. marginata, Ammonia spp. and N. auris) show a lighter carbon isotopic excursion with respect to the epifaunal to shallow infaunal forms (C. wuellerstorfi, L. amygdalaeformis and B. seminuda). These lighter \(\updelta ^{13}\) \(\hbox {C}\) values are related to utilisation of \(\hbox {CO}_{2}\) produced by anaerobic remineralisation of organic matter. However, enrichment of \(\updelta ^{18}\) \(\hbox {O}\) for the deeper microhabitat (bearing lower pH and decreased \({\hbox {CO}_{3}}^{2-})\) is only recorded in case of B. marginata. It is reverse in case of N. auris and related to utilisation of respiratory \(\hbox {CO}_{2}\) and internal dissolve inorganic carbon pool. Estimation of interspecies isotopic correction factors for the species pairs (\(\updelta ^{13}\) \(\hbox {C}\) of C. wuellerstorfi–B. marginata, L. amygdalaeformis–Ammonia spp., N. auris–B. seminuda) and \(\updelta ^{18}\) \(\hbox {O}\) of C. wuellerstorfi–B. marginata are statistically reliable and may be used in palaeoecological studies.     

Simplified method for calculating inflow into a deep excavation with consideration of the effects of cutoff walls

Water inflow into deep excavations is a key parameter in the evaluation of environmental impact, and a simplified method is needed to calculate the inflow with adequate accuracy, especially for a deep excavation with a cutoff wall around it. This paper presents a model for calculating inflow based on the method of fragments, assuming two-dimensional sectional flow. The effects of cutoff wall thickness (w), wall penetration, and excavation width on the inflow are well considered in this model. Explicit formulas for the form factor are given. The accuracy and scope of this method are discussed. The method can be applied in both flooding and no flooding cases. Extensive verification shows that, for the flooding case and when one of four conditions is satisfied, the error of the proposed method will be within 10%. These conditions are b/d?≥?0.8 or w/d?≥?0.03 or s/d?≥?0.2 or s₁/d?≥?0.2, for which b is the half width of the excavation, d is the thickness of the soil layer underlying the wall, and s and s₁ are the wall penetration depths on the non-excavation side and excavation side, respectively. For the case of no flooding and when the ratio of the distance from the recharge boundary to the aquifer thickness (a/T) is?≥2, the error does not exceed 15%.     

Detailed stratigraphy of the Jurassic-Cretaceous boundary beds of the Lena River lower reached based on ammonites and Buchiids

The Jurassic-Cretaceous (Volgian-Lower Valanginian) boundary deposits in the lower reaches of the Lena River (near the village of Chekurovka and on the Cape Chucha, North Yakutia) are described bed-by-bed. The taxonomic composition of ammonites and bivalves (mainly Buchia) is determined. The species assemblages are biostratigraphically analyzed and biostratigraphic units of the bed and zone ranks are recognized based on by Buchia and ammonites. Analogues of the Buchia Zones (buchiazones) Unschensis, Okensis, Tolmatschewi, Inflata and ammonite zones Exoticus, Okensis, Sibiricus, Analogus, Mesezhnikowi and ? Klimovskiensis are established in this region for the first time. A correlation of the distinguished buchiazones with the buchiazones of the Nordvik Peninsula, New Siberian Islands (Stolbovoy Island), the basin of the Anyuy River, and Northern California is proposed. The ammonite assemblages constantly contain phylloceratids; some stratigraphic intervals also contain lytoceratids; this is probably connected with a Paleo-Pacific influence. Unlike the ammonite assemblages of the same age of the Khatanga depression, the subzones cannot be distinguished in the Sibiricus and Kochi Zones of the Lower Lena due to differences in the stratigraphic ranges of some ammonite species. Hectoroceras in the Kochi zone is very rare and Praetollia predominates here.