二长棘鲷和黄鳍鲷骨骼系统的比较

对二长棘鲷和黄鳍鲷的骨骼系统进行了比较研究。结果表明,二长棘鲷脑颅中的上筛骨、侧筛骨、额骨、眶下骨、蝶耳骨、上枕骨,咽颅中的前上额骨、腭骨、舌额骨、齿骨、鳃盖骨、尾舌骨、咽鳃骨等与黄鳍鲷相应部分的骨骼有明显差异,某些特征可作属间或种间的鉴别依据。     

Molecular Phylogenetic Lineage of Plagiopogon and Askenasia (Protozoa,Ciliophora) Revealed by Their Gene Sequences

Prostomates and haptorians are two basal groups of ciliates with limited morphological characteristics available for taxonomy. Morphologically, the structures used to identify prostomates and haptorians are similar or even identical, which generate heavy taxonomic and phylogenetic confusion. In present work, phylogenetic positions lineage of two rare genera, Plagiopogon and Askenasia, were investigated. Three genes including small subunit ribosomal RNA gene(hereafter SSU r DNA), internal transcribed spacer region(ITS region), and large subunit ribosomal RNA gene(LSU r DNA) were analyzed, 10 new sequences five species each. Our findings included 1) class Prostomatea and order Haptorida are multiphyletic; 2) it may not be appropriate to place order Cyclotrichiida in subclass Haptoria, and the systematic lineage of order Cyclotrichiida needs to be verified further; 3) genus Plagiopogon branches consistently within a clade covering most prostomes and is basal of clade Colepidae, implying its close lineage to Prostomatea; and 4) Askenasia is phylogenetically distant from the subclass Haptoria but close to classes Prostomatea, Plagiopylea and Oligohymenophorea. We supposed that the toxicyst of Askenasia may be close to taxa of prostomes instead of haptorians, and the dorsal brush is a more typical morphological characteristics of haptorians than toxicysts.     

A study on the flora of woody plants of Shanxi and the relationship among the flora of Shanxi and some regions, China

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A study on the flora of woody plants of Shanxi and the relationship among the flora of Shanxi and some regions, China

There are 463 species of woody plant, belonging to 166 genera and 70 families in Shanxi, North China. The floristic composition types of genera and species are abundant, in which the compositions distributed in temperate zones (including North Temperate, Old World Temperate and Temperate Asia), having 62 genera, are dominant and account for 38.27% in total genera (except for Cosmopolitan), and they are major constructive or dominant species of vegetation in Shanxi. The floristic compositions of woody plants of Shanxi, Hebei, Beijing, Shandong, Henan, Jiangxi and Inner Mongolia were studied comparatively by using diversity index, principal component analysis (PCA) and group-average clustering, respectively. The floristic composition of Shanxi was considerably similar to that of Hebei, Beijing and Shandong because they shared a lot of genera, specially genera distributed in temperate regions, such as Rosa, Quercus, Populus, Salix, Acer, Ulmus, Pinus etc. and their eco-environment were considerably similar. However, the flora of Shanxi was comparatively different from that of Henan, Jiangxi and Inner Mongolia by reason that there were distinct differences among their eco-geographic conditions, floristic compositions and constructive or dominant species of dominant vegetation types. Supported by Shanxi Returned Oversea Scholar Foundation, China     

Molecular Grouping of Grateloupia Tissues Collected Along Chinese Coast and Microsatellite Diversity Analysis of G. asiatica

Genus Grateloupia is one of the most speciose genera in family Halymeniales. It is also one of the sources for natural materials, food and medicine. With different environments, Grateloupia change their morphological characteristics, making their morphological identification very difficult. In addition, few of the species diversity in this genus has been described before. In this study, phylogenetic analysis based on rbc L gene sequence was employed to group Grateloupia collected from three locations along Chinese coast. The microsatellites were also used to evaluate their genetic diversity. In total, the tissue parts of 6 putative species were collected from G. asiatica, G. livida, G. lanceolate, G. catenata, G. turuturu and G. filicina. In order to evaluate their genetic diversity and then conserve them better, 40 microsatellites available for Grateloupia were used to evaluate their genetic diversity, and 11 microsatellites were found to be applicable to determine the genetic diversity of G. asiatica. It was found that the genetic diversity of G. asiatica around Qingdao was very rich. We suggested that the species of genus Grateloupia should be identified based on rbc L phylogenetic analysis before the diversity evaluation with microsatellites. The microsatellites should be developed for each species of Grateloupia so that their genetic diversity can be evaluated appropriately.     

Identification of Microplankton in South China Sea with Image-Matching Individual PCR

In this study, marine microplankton were identified by combining standard light microscopy with Sanger 18S rRNA gene sequencing. The image-matching individual PCR technique was applied to identify the image collectable unicellular microplankton to genera. Instead of pure strain culture and morphological identification, microplankton individual cells were isolated and fixed with glutaraldehyde, frozen and stored for months. Finally, they were imaged under a microscope and molecularly identified via phylogenetic analysis of their 18S ribosomal RNA gene(18S rDNA). Microplankton cells were collected at 30 locations in South China Sea, and were assigned to 21 known and 4 unidentified genera(2 uncultured fungi and 2 uncultured stramenopiles) with phylogenetic analysis in parallel to the morphological identification.     

Monophyly or polyphyly? Possible conflict between morphological and molecular interpretations of the wellknown genus Zoothamnium(Ciliophora,Peritrichia)

In this paper,we explore possible conflict between morphological and molecular interpretations of phylogenetic relationships within the well-known peritrichous genus Zoothamnium. On the basis of morphological evidence,for a long time this genus has been believed to be a well-defined monophyletic taxon. Nonetheless,Z oothamnium exhibits higher genetic diversity than the gross morphology of its species. Here,we used all available genetic information for the small subunit of ribosomal RNA(SSU r RNA) and internal transcribed spacer region(ITS1-5.8S-ITS2) for this genus to reconstruct phylogenies for four datasets(SSU rRNA,ITS1,ITS2,and ITS1-5.8S-ITS2) and a combined dataset(SSU rRNA+ITS1-5.8SITS2) using different phylogenetic methods and with consideration of the secondary structure of the genes. Confidence in phylogenetic tree selection was assessed with the approximately unbiased test. The molecular results showed both that Zoothamnium is more likely to be polyphyletic,and morphologically similar genera Zoothamnopsis and Myoschiston were always nested among Zoothamnium species. Accordingly,as with some other groups of ciliates,to understand more fully the correct phylogeny of Zoothamnium there remains a need for additional data from both morphological and molecular studies,covering additional Zoothamnium spp. and members of closely related genera(e.g. Zoothamnopsis,Myoschiston,and Epistylis).     

Establishment of Alloptilella splendida gen.et sp.nov.and resurrection of Scytalium veneris(Thomson & Henderson,1906),two sea pens(Cnidaria:Pennatulacea) from seamounts in the tropical Western Pacific

Sea pens are a highly specialized and morphologically distinct group of octocorallians,but many taxa were poorly described and their phylogenetic relationships are still poorly known.We describe two species of sea pens collected from the tropical Western Pacific:Alloptilella splendida gen.et sp.nov.and Scytalium veneris(Thomson Henderson,1906).Alloptilella splendida gen.et sp.nov.accords with the definition of the recently resurrected genus Ptilella Gray,1870 in most characters,but differs from the latter in the arrangement of autozooids and the location of mesozooids.The new species mostly resembles Pennatula naresi K(o|")lliker(1880),but differs by the location of me sozooids and the reddish color of sclerite s surrounding siphonozooids.The generic separation is also well supported by their genetic distances and the molecular phylogenetic trees constructed by the concatenated mtMutS-COI-28 S,where Alloptilella clustered with Scytalium Herklots,1858 and formed a clade with the cluster of Ptilella and Distichoptilum.Scytalium veneris,usually considered as a junior synonym of Scytalium sarsii Herklots,1858,is resurrected by recognizing their distinctive differences.This is the first record of Scytalium veneris outside its type locality,and the phylogenetic analysis indicates that Scytalium is a monophyletic group.Both the families Pennatulidae and Virgulariidae are polyphyletic,and more morphological and molecular data are needed to clarify the phylogenetic positions of pennatulacean families and genera.     

Morphology and SSU rDNA sequence analysis of two hypotrichous ciliates (Protozoa,Ciliophora, Hypotrichia) including the new species <Emphasis Type="Italic">Metaurostylopsis parastruederkypkeae</Emphasis> n. sp.

The morphology and phylogeny of two hypotrichous ciliates, Metaurostylopsis parastruederkypkeae n. sp. and Neourostylopsis flavicana (Wang et al., 2011) Chen et al., 2013 were investigated based on morphology, infraciliature and the small subunit (SSU) ribosomal RNA gene (rRNA) sequence. The new species, M. parastruederkypkeae n. sp. was identified according to its characteristics: body shape ellipsoidal, size about (165–200) × (45–60) μm in vivo, cell color reddish; two types of cortical granules including wheat grain-like and yellow-greenish larger ones along the marginal cirri rows and dorsal kineties and dot-like and reddish smaller ones, grouped around marginal cirri on ventral side and arranged in short lines on dorsal side; 26–41 adoral membranelles; three frontal and one parabuccal, five to seven frontoterminal, one buccal, and three to six transverse cirri; seven to thirteen midventral pairs; five to nine unpaired ventral cirri, five to seven left and three to five right marginal rows; and three complete dorsal kineties. Phylogenetic analysis based on SSU rDNA sequences showed that both Metaurostylopsis and Neourostylopsis are monophyletic. As the internal relationship between and within both genera are not clear, further studies on the species in these two genera are necessary. The key characteristics of all known twelve Metaurostylopsis-Apourostylopsis-Neourostylopsis species complex were updated.     

Fauna and standing crop of freshwater mussels in Poyang Lake, China

Freshwater mussels are a major component of the zoobenthos and they therefore play an important role in freshwater ecosystems. The community composition and the standing crop of freshwater mussels in Poyang Lake, the largest freshwater lake in China and one of two lakes connected to the Changjiang (Yangtze) River, were investigated from February 2008 to May 2009. Mussels were sampled in hydrologically and geographically distinct sampling areas. A total of 42 species and 13 genera were identified, of which 32 species were endemic to China. Two species, Unio douglasiae and Lamprotula caveata, dominated all sampling areas. The number of genera and species, and the number of species within each genus, differed significantly among sampling areas. Freshwater mussels were very abundant, with an overall density of 0.28 ± 0.22 ind./m2 and biomass of 4.08 ± 3.96 g/m2. Significant variation observed among sampling areas and among genera was probably due to the variety of habitats among the areas sampled and to the adaptability of different freshwater mussels to environments. This study suggests that Poyang Lake and other freshwater lakes in the region are important sites of freshwater mussel biodiversity and conservation.     

Morphological Studies on the Infraciliature of a Planktonic Ciliate, Tintinnopsis brasiliensis （ Ciliophora ： Tintinina）

A poorly-described marine planktonic ciliate, Tintinnopsis brasiliensis Kofoid ＆ Campbell, 1929, collected from the Taiping Cape of Qingdao, China, was morphologically investigated based on permanent preparation after protargol impregnation and was compared with other related congeners. According to the infraciliature, three ciliary groups can be recognized, which reveals a very stable structure among specimens and denotes that the pattern of infraciliature is, apart from the features of the lorica, a highly reliable criterion for species identification.     

Molecular phylogeny of the genus Clibanarius(Decapoda: Anomura: Diogenidae) based on mitochondrial and nuclear DNA sequences

Clibanarius,a genus of hermit crabs,is one of the most diverse genera within Diogenidae.However,studies on the phylogeny within Clibcanarius is very limited.We reconstruct the molecular phylogeny of the genus Clibanarius based on two mitochondrial(16 S rRNA and COI)and two nuclear protein-coding(NaK and PEPCK)genes using a multigene phylogenetic approach.Eleven selected Clibanarius species from the Indo-West Pacific are analyzed and the Bayesian and maximum likelihood analyses produced identical results in topology.Results suggest that C.rutilus and C.infraspinatus are more closely related to each other than to C.snelliusi,and C.striolatus shows a close relationship to C.longitarsus.The close association of C.merguiensis with C.englaucus and C.humilis is strongly supported by the divergence of C.virescens from them.Our phylogenetic results contradict the morphological classification scheme proposed for Clibanarius and indicate that the relative length of dactyls and propodi is not phylogenetically significant in Clibanarius.In addition,we speculate that the morphological characteristic of the median cleft of the telson might be phylogenetically important for Clibanarius.     

A comparative study of fish community in four main estuaries of China southeastern coastal areas and their adjacent waters

Based on literature and survery data of fish composition collected by bottom trawl investigation from 2006 to 2007 infour main estuaries of China southeastern coastal areas and their adjacent waters,changes of the taxonomic diversity across spatialand temporal scales of fish community were analyzed by taxonomic diversity indices.The results are as follows:a total number of1397 fish species(including some freshwater species),belonging to 2 classes,42 orders,186 families and 593 genera,were collected inthe studied sea areas.The species richness increased with lower latitudes,particularly so with Perciformes.There were 339 fish spe-cies in the Yangtze River Estuary and adjacent waters,belonging to 2 classes,31 orders,101 families and 231 genera.There were 535fish species in the Minjiang River Estuary and adjacent waters,belonging to 2 classes,33 orders,133 families and 323 genera.A totalnumber of 803 fish species were collected in the Jiulongjiang River Estuary and adjacent waters,which belonged to 2 classes,35 orders,155 families and 419 genera.And 1021 fish species which belonged to 2 classes,32 orders,153 families,and 466 genera were collectedin the Pearl River Estuary and adjacent waters.The numbers of orders and families of fish species from the northern to the southern wa-ters first increased and then decreased.The average variation in taxonomic distinctness(Λ+)gradually decreased with lower latitudes inthe four estuaries and adjacent waters.There were no significant differences in the average taxonomic distinctness(△+)among the fourestuaries and adjacent waters,and fish fauna were closely related with each other,and all of them belonged to the same zoogeographicalfauna(Indian-Malaysia fauna).Fish composition in the Minjiang River Estuary and adjacent waters was more similar to that in the Jiu-longjiang River Estuary and adjacent waters among the four estuaries.Compared with the historical data,the average taxonomic dis-tinctness of fish community showed a great decrease in the Minjiang River Estuary and the Jiulongjiang River Estuary and their adjacentwaters.     

Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam, the Philippines and Indonesia. Combined with morphological characteristics, all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition, the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds, while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%. Eucheumatoideae algae cultivated in China consisted of three clades, K. alvarezii, Kappaphycus sp., and E. denticulatum. The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.     

A new member of Sphenopsida,Neolobatannularia gen. nov. from Late Triassic of western Liaoning,China

A new genus Neolobatannularia Sun et Li gen. nov. with the type species Neolobatannularia liaoningensis Sun et Li sp. nov. from the Upper Triassic Yangcaogou Formation of Beipiao, Liaoning, China, is described in this paper. The new taxon is attributed to Equisetales of Sphenopsida based on its branch with internodes, node and leaves borne in whorls on nodes. It is a new member of the Mesozoic Sphenopsida. Although the new taxon shares some morphological characters with the genus Lobatannularia Kawasaki, a genus widely distributed in the Permian Cathaysia flora in East Asia, and the genus Lobatannulariopsis Yang from the Late Triassic of southwestern China, its unique branching pattern is markedly distinguished from the two known genera. Some Triassic known species of Lobatannularia may belong to the present new genus Neolobatannularia.