Mercury Circulation in Aquatic Bioconeses. Part 1: Mercury (II)-Metabolism in Phytoplankton

The metabolism of inorganic mercury has been studied in stationary cultures of planktonic algae Chlorella kessleri, Scenedesmus obliquus and Microcystis incerta that were exposed for 14 days to the concentration of 2 × 10^?7 M of Hg(II). Individual chemical forms of mercury were determined in algae and in the culture medium at regular intervals. All the studied phytoplankton species started to metabolize mercury immediately after its application, with the exception of the blue-green alga Microcystis incerta. The accumulation of mercury proceeds without interruption till the end of the experiment. After a 14 days cultivation about 60… 80% of added mercury was present in algal cells and the portion of mercury washable from the cell surface did not exceed 2%. It was found that more than 99,5% of mercury in cultivation medium and more than 99,5% of mercury in algae was present in the form of Hg(II). A transformation of inorganic mercury to methyl-, or phenyl-mercury due to the action of algae was not observed.     

The Uptake of 226Ra by Planktonic Algae under Conditions of Continuous Cultivation

The radium cumulation in Coelastrum cambricum, Scenedesmus obliquus and Microcystis pulverea was studied under conditions of continuous cultivation at constant level of radium in the cultivation medium. The algae were exposed to the concentrations of 30, 300 and 3000 Bq/l ²²⁶Ra in the medium for a period of 14 days. It was found that radium started to cumulate immediately after its addition to the culture just as in the stationary type of cultivation, in the continuous cultivation, however, its content decreased with the length of exposure. This is due to the decreasing part of radium adsorbed on the cell surface. While in green algae the portion of radium incorporated into cells increased with the length of exposure, in blue-green algae up to 90% of radium was bound to the surface or gelatinous capsules of the cells through out the experiment. With the three phytoplankton species used, the highest cumulation of radium (nonwashable part) was recorded in Coelastrum cambricum. A dependence of the radium cumulation on its content in the medium was observed in the species Scenedesmus obliquus.     

实时荧光定量PCR方法检测南太湖入湖口产毒微囊藻

南太湖入湖口产毒微囊藻的丰度对于周边县市取水口的水质安全和太湖水质有着重要影响.以藻毒素合成酶基因mcyE/ndaF为靶基因,建立实时荧光定量PCR检测产毒微囊藻的方法,并对南太湖入湖口7个监测点水样中产毒微囊藻的丰度进行检测,结果表明:该实时荧光定量PCR方法的特异性强及准确性、重复性较好.建立铜绿微囊藻(Microcystis aeruginosa)的标准曲线方程为y=-3.454x+49.88,斜率为-3.454,R~2=0.991,扩增效率E为94.6%,定量检测区间为1.689×10~4~1.689×10~8拷贝数/μl.对南太湖入湖口7个监测点检测表明,夹浦和合溪2个监测点的产毒微囊藻数量最高,预测产毒微囊藻浓度分别为(1.99±0.35)×10~5和(1.47±0.23)×10~5cells/ml.7个监测点的产毒微囊藻的种类较为一致,均为铜绿微囊藻.该方法可以快速准确地检测水体中微囊藻毒素产毒藻种种类和数量,为蓝藻水华监测、预警提供技术依据.     

Bioreduction of Hexavalent Chromium by Live and Active Phanerochaete chrysosporium: Kinetics and Modeling

In this work the potential of live and active Phanerochaete chryosporium, a white rot fungi, to remove lower Cr(VI) concentration from aqueous solutions was reported for the first time. A medium pH had significant effect on the growth of the fungus and bioremoval of Cr(VI). Substrate inhibition on the growth of Phanerochaete chrysosporium was evident beyond 20 g L^?1 of dextrose concentration. A maximum biomass concentration of 15.64 g L^?1 was obtained for an initial dextrose concentration of 20 g L^?1 in metal free medium at pH 6.0. An increase in Cr(VI) concentration beyond 10 mg L^?1 inhibited the growth of the fungi, thereby, reducing the chromium bioremoval efficiency. A maximum reduction efficiency of 98.92% was reported for an initial metal concentration of 10 mg L^?1. A mathematical expression for the bioreduction of Cr(VI) considering the organic compounds in the cells was proposed.     

Mercury Circulation in Aquatic Environment. Part 2: Metabolism of Methyl and Phenyl Mercury in Phytoplankton

Biotransformation of phenyl and methyl mercury chloride was studied in experiments with planktonic algae Chlorella kessleri, Scenedesmus obliquus and blue-green algae Microcystis incerta. To algal cultures cultivated in an inorganic medium the labelled phenyl or methyl mercury hydroxide was added separately in the phase of the logarithmic growth, the resultant concentration being 3.2 × 10^?7 M. In intervals of 0, 1, 3, 7, 10 and 14 days samples of the algae and medium were analysed for the total amount of mercury and for the presence of phenyl or methyl mercury. It was found that both methyl and phenyl mercury had accumulated very rapidly in the algae and that up to 70% of ²⁰³Hg added was present in the algae during the first 72 hours of the experiment. From the 2nd day onwards, however, methyl and phenyl mercury changed rapidly into inorganic mercury so that by the end of the experiment almost all of the mercury present in algae and the medium was in the form of inorganic mercury.     

辽河太子河流域藻类植物群落结构及其季节变化

2010年9月到2011年9月分4次对太子河流域的藻类植物群落组成、丰度及优势种进行调查分析.共设置17个采样点,经鉴定共有藻类植物8门120属328种(包括21种变种).藻类植物群落以硅藻最多,有36属144种,占总体的43.77%;其次是绿藻,有49属117种,占总体的35.56%;蓝藻居第3位,有22属38种,占总体的11.85%.调查期间,太子河流域藻类植物细胞丰度在夏、秋季较高,丰度平均值分别为6.80×10⁷和3.29×10⁷cells/L;在春季较低,丰度平均值为1.36×10⁷cells/L.优势度分析显示,太子河流域各季节的藻类植物优势种都在5种以上,主要优势种有巨颤藻(Oscillatoria princeps Vauch.)、链丝藻(Hormidium flaccidum(Kütz.)Br.)、偏生毛枝藻(Stigeoclonium subsecundum(Kütz.)Haz.)等.藻类植物的Jaccard种类相似性指数在0.19~0.46之间.太子河流域藻类植物种类组成及细胞丰度均有明显的季节变化.     

Bioremediation of Tannery Effluents Using a Consortium of Blue–Green Algal Species

Trivalent chromium in the form of basic chromium sulfate (BCS) is used for tanning hides/skins and is a strong pollutant of the soil and water bodies. Significant quantities of unutilized chemicals, such as sulfates, chlorides, are also discharged, contributing to high levels of total dissolved solids (TDS), biochemical oxygen demand (BOD), chemical oxygen demand (COD), etc. Though many treatment techniques are being practiced, biotechnological methods are gaining importance. Biosorption is recognized as a cost‐effective technology worldwide; one potential sorbent being blue–green algae (BGA), for treating metal‐bearing effluents. This work studies the feasibility of using a species each of Spirulina, Oscillatoria, and Synechocystis, individually and as a consortium, as sorbents to remove Cr³⁺ from a segregated stream, viz. exhaust chrome liquor (ECL) and synthetic BCS solution. The species studied were found to be effective in removing Cr³⁺ considerably at varying concentrations, besides reducing sulfates, BOD, COD, etc. The results of ECL experiments were more encouraging than those for BCS solution. The kinetic data on Cr³⁺ sorption onto algal biomass fit well into the pseudo‐second order model. The equilibrium data were analyzed using the classic Langmuir and Freundlich isotherm models, yielding good fits. The results of the experiments indicate that algal consortia could be good alternatives to the conventional treatment methods for leather and other industrial wastewaters containing chromium.     

Chemical Reduction of Hexavalent Chromium Present in Contaminated Soil using a Packed-bed Column Reactor

Soil samples extracted from a contaminated landfill with hexavalent chromium, Cr(VI), were treated in a packed-bed column reactor applying different reductant solutions under controlled flow conditions. In all cases, the kinetic study concerning removal of Cr(VI) from packed soil revealed the overall process (leaching and redox reaction) is described by pseudo-first order kinetics. The complexity of the process taking place in non-isotropic medium (packed soil) was evidenced by dependence of the overall pseudo-first order kinetic rate constant, k*, on treatment time, volumetric flow rate, and composition and concentration of the reductant solution. A phenomenological equation was proposed in order to represent k* in non-ideal conditions. The comparative study concerning the use of different reductants revealed application of ferrous sulphate, Fe(II), under flow conditions furnished the best results, since in this case the redox process reached a considerable extent and the reduced chromium, Cr(III), was immobilized in packed soil as an insoluble mixed compound.     

Some aspects of vanadium and chromium chemistry in the English Channel

Dissolved and particulate vanadium and chromium concentrations along a transect between Cherbourg and the Isle of Wight were investigated in the English Channel. Seawater samples were collected at two different depths (surface and bottom) at five sampling stations during five cruises carried out between September 1994 and September 1995. Calculated mass flows through this Channel section were 7600 T yr⁻¹ for vanadium (about 66% was in the dissolved phase) and 1650 T yr⁻¹ for chromium (about 50% was in the dissolved phase). Dissolved chromium concentrations do not vary significantly along the transect. Seasonal variations in chromium distribution linked to biotic parameters were noted during the September 1994 cruise, when a significant relationship between particulate chromium and algal organic carbon was found. In addition, dissolved Cr (III) and Cr (VI) were well correlated with both algal and terrestrial organic matter. In the May cruise, during phytoplanktonic decay, particulate chromium was correlated with the detritic and bacterial organic fractions. These observations suggest interactions between chromium and biotic material. During the winter period, no relationship between chromium and POC was found. Dissolved and particulate vanadium concentrations varied, respectively, between 15 and 28 nmol l⁻¹ and 2–32 nmol l⁻¹. Values of dissolved vanadium showed depletion in the Channel with respect to oceanic waters. This loss in dissolved vanadium was higher near the English coast, but was compensated for by the increase in the particulate vanadium. There was no clear relationship between dissolved vanadium and algal organic carbon and it is inferred that the vanadium transfer cannot be explained by trapping with biotic material. On the other hand, the dissolved vanadium depletion could be attributed to the presence of ferric oxyhydroxide phases in particles, which have strong adsorbing properties for a range of dissolved metal ions.     

Growth of Plankton (Scenedesmus acutus (Chlorophyceae) and Moina macracopa (Cladocera)) on Domestic Wastewater

The population growth of the freshwater chlorophyte, Scenedesmus acutus and the cladoceran Moina macrocopa cultured in the laboratory and in microcosms of a wastewater treatment plant was studied. S. acutus was cultured in a defined algal medium and mixed with wastewater. On diets of S. acutus cultured using Bold medium or a mixture with wastewater, the peak densities of M. macrocopa were 6–7 ind. mL^?1, while on a diet of algae cultured on wastewater alone the cladoceran density fluctuated around 2 ind. mL^?1. Population growth rates of M. macrocopa, which ranged from 0.3 to 0.4 d^?1, were significantly lower on alga cultured in wastewater alone. The body size structure of M. macrocopa was larger on algae cultured in Bold medium than in wastewater. Peak densities of M. macrocopa were around 700 ind. L^?1 when the organic matter in the containers was supplemented with algae but it reached lower densities (400 ind. L^?1) when cultured on the organic matter in wastewater alone. Our study indicates that the nutrients in the final stages of wastewater treatment can support the growth of both phytoplankton and zooplankton.     

Removal of Chromium from Synthetic Effluent using Nymphaea rubra

In the present work, biosorption of Cr(VI) by Nymphaea rubra was investigated in batch studies. Batch experiments were conducted to study the effect of initial sorbent dosage, solution pH and initial Cr(VI) concentration. The results showed that the equilibrium uptake capacity was increased with decrease in biomass dosage. The Cr(VI) removal was influenced by the initial chromium compound concentration. Langmuir and Freundlich adsorption isotherm models were used to represent the equilibrium data. The Freundlich isotherm model was fitted very well with the equilibrium data when compared to Langmuir isotherm model. The sorption results were analyzed for pseudo‐first order and pseudo‐second order kinetic model. It was observed that the kinetic data fitted very well with the pseudo‐second order rate equation when compared to the pseudo‐first order rate equation. Fourier transform infrared spectrum showed the presence of different functional groups in the biomass. The surface morphology of the sorbent was exemplified by SEM analysis. Aquatic weeds seem to be a promising biosorbent for the removal of chromium ions from water environment. This paper reports the research findings of a laboratory‐based study on the removal of Cr(VI) from the synthetic solution using the dried stem of N. rubra as a biosorbent.     

三峡水库澎溪河典型优势藻原位生长速率的初步研究

本文提出了两种原位培养装置(培养笼、培养桶)并采用这两种装置在澎溪河高阳平湖断面进行原位培养实验,旨在研究澎溪河典型优势藻的原位生长速率.研究期间主要生境特征为营养物丰足、光热条件优越、生境条件相对稳定,适宜于藻类生长,为水华形成的敏感期;在此期间代表CR型生长策略的湖北小环藻和兼具C、R、S生长策略的卵形隐藻的原位生长速率总体呈逐渐下降趋势,代表CS型生长策略的水华鱼腥藻和空球藻的原位生长速率则先增加后又有所下降,代表S型生长策略的铜绿微囊藻的原位生长速率虽然前期都为负,但总体呈现逐渐增加的趋势;并测得培养笼和培养桶中各藻种的最大比生长速率分别为湖北小环藻0.31、0.21 d-1;铜绿微囊藻0.09、0.03 d-1;空球藻0.16、0.42 d-1;水华鱼腥藻0.30、0.26 d-1;卵形隐藻0.49、0.95 d-1.本研究中,不同生长策略藻种生长速率的变化在一定程度上体现了不同生长策略藻种的生长趋势.另外,就装置性能实验及原位培养实验结果综合分析,认为两种原位培养装置均能够满足对天然水域藻类原位生长速率的测试要求,但装置的构造对藻类实际生长过程仍存在一定影响.     

Phytoplankton Distribution in an Oligotrophic Pond and a Eutrophic Pond

Summary: An oligotrophic pond and a eutrophic pond of Bhagalpur, India, were studied for phytoplankton distribution and to establish the relationship between this distribution and the physico-chemical properties of the water. Altogether, 114 algal taxa were identified. In Rekabganj pond, diatoms constituted 54.4 % of the algal species followed by green algae (29.4 %), blue greens (11.7 %) and euglenoids (2.9 %). In Ramna pond, blue greens were the most dominant comprising 35.7 % of algal species followed by green algae (32.8 %), diatoms (18.5 %) and euglenoids (8.5 %). The members of Dinophyceae, Chrysophyceae and Xanthophyceae were poorly represented in both the water-bodies. Aphanizomenon flos-aquae, Microcystis aeruginosa, Chlorella vulgaris, Cladophora crispata, Volvox aureus and Euglena spp. were recorded only in Ramna pond, indicating its higher nutrient status. Dinobryon calciformis, many pennate diatoms and desmids, indicators of oligotrophy, were observed in Rekabganj pond only. Measurements of water properties in the two ponds showed that transparency, dissolved oxygen and silicate were higher in Rekabganj pond than in Ramna pond, while total alkalinity, hardness, total dissolved solids, dissolved organic matter, different cations, phosphate, nitrate and chloride were lower in Rekabganj pond than in Ramna pond.     

基于遥感反射率的太湖优势藻识别方法

我国淡水湖库频发水华,不同类群形成的水华特征、危害及其治理方法差异显著,因此,如何区分不同藻种的遥感反射率特征,获取湖泊优势种信息是一个亟待解决的科学问题。研究基于室内藻种培养实验,培养了富营养化湖泊中的典型蓝藻和绿藻藻种,其中,蓝藻包括铜绿微囊藻（Microcystis aeruginosa）、假鱼腥藻（Pseudanabaenasp．）和束丝藻（Aphanizomenonsp．）,绿藻包括小球藻（Chlorellasp．）以及四尾栅藻（Scenedesmus quadricauda）;基于实测的遥感反射率,经归一化处理后,分析了不同藻种的遥感反射率特征,构建了DI(difference index)指数以及ADI(algae distinguish index)指数,建立了藻种分类模型,利用验证集数据进行检验,整体识别精度达77.55%,Kappa系数为0.7178。将分类方法应用于太湖野外实测遥感反射率数据集中,结果与实测的生物量数据有较好的匹配;将模型应用于太湖OLCI(ocean and land colour instrument)影像数据,获得了2019年12月和2020...     

Semicontinuous Outdoor Cultivation of Scenedesmus acutus in Waste Water

The algae were cultivated in an outdoor cultivation unit in waste water from sewage treatment plant processing city sewage and largescale hoggery effluent. The cultivation area (2m²) had a slant of 3% and the suspension layer thickness was about 5 cm. The total suspension volume in the cultivation device was 150 I. Mass balance served us for derivation of formulas for the average rate of algal biomass production and for the extent of nutrient removal from waste water. Experiments showed a considerable effect of dilution rate on individual parameters in these formulas. The removal of nitrogen and phosphorus is optimal at a dilution rate of 0.3 day^?1 whereas optimum biomass production lies at about 0.1 day^?1. The nitrogen and phosphorus yield coefficient Y (g biomass/g element) are practically identical, both of them depending on dilution rate. The effect of the dilution rate on other characteristics of the effluent water has not yet been unambiguously proved. The results are shown of bacteriological and mycological examinations, in addition, of the chemical analysis of resulting algal biomass.     

Cumulation of Zinc,Cadmium and Mercury on the Alga Scenedesmus obliquus

In batch experiments with the metals labelled with their radionuclides the cumulation of zinc, cadmium and mercury on Scenedesmus obliquus in dependence on the pH-value is measured, because the original pH-value of the solution is changed by the algae due to their absorption of hydrogen-ions. For the uptake of zinc and cadmium the logarithm of the cumulation factor in the range of 2 … 5 is a linear function of the pH-value in the range of 5 … 9, cumulation increasing with rising pH-value. In contrast, the mercury cumulation is pH-independent. If the changed pH-value is taken into account, the cumulation factors are independent of the metal concentration in the range = 10^?4 mol/l. The degree of cumulation is not changed by the presence of other metals. The cumulation factor is reduced by substances forming negatively charged complexes with the metals.     

Population Growth of Zooplankton (Rotifers and Cladocerans) Fed Chlorella vulgaris and Scenedesmus acutus in Different Proportions

In the present work we tested the effect of Chlorella vulgaris and Scenedesmus acutus in different proportions on the population growth of Brachionus calyciflorus, Brachionus patulus, Ceriodaphnia dubia, and Moina macrocopa. In general, both rotifer species grew well on either type of algae. Regardless of algal mixture, B. calyciflorus had a shorter initial phase, while B. patulus needed more than a week to begin the exponential phase of growth. Both the rotifer species showed consistently better population growth with Chlorella than on Scenedesmus, or the mixture. At any given algal combination, B. patulus had higher peak values than B. calyciflorus. The rate of population increase (r) for both the rotifers varied from 0.18 to 0.48 d^—1 depending on the algal type and combination. Regardless of algal type and combination, B. calyciflorus had a much higher value of r than B. patulus. Both C. dubia and M. macrocopa grew on the algal types, whether offered separately or in mixture. Regardless of the treatment type, C. dubia needed a longer period (about 2 weeks) than M. macrocopa to reach peak abundances. Thus, our study did not support the view that Scenedesmus is consistently superior to Chlorella as a basic diet to the tested species of zooplankton.     

Chromium Removal through Biosorption and Bioaccumulation by Bacteria from Tannery Effluents Contaminated Soil

Four bacterial isolates (two resistant and two sensitive to chromium) were isolated from soil contaminated with tannery effluents at Jajmau (Kanpur), India, and were identified by 16S rDNA gene sequencing as Stenotrophomonas maltophilia, Exiguobacterium sp., Pantoea sp., and Aeromonas sp. Biosorption of chromium by dried and living biomasses was determined in the resistant and sensitive isolates. The effect of pH, initial metal concentration, and contact time on biosorption was studied. At pH 2.5 the living biomass of chromium resistant isolate Exiguobacterium sp. ZM‐2 biosorbed maximum amount of Cr⁶⁺ (29.8 mg/g) whereas the dried biomass of this isolate biosorbed 20.1 mg/g at an initial concentration of 100 mg/L. In case of chromate sensitive isolates, much difference was not observed in biosorption capacities between their dried and living biomasses. The maximum biosorption of Cr³⁺ was observed at pH 4.5. However, biosorption was identical in resistant and sensitive isolates. The data on chromium biosorption were analyzed using Langmuir and Freundlich isotherm model. The biosorption data of Cr⁶⁺ and Cr³⁺ from aqueous solution were better fitted in Langmuir isotherm model compared to Freundlich isotherm model. Metal recovery through desorption was observed better with dried biomasses compared to the living biomasses for both types of chromium ions. Bioaccumulation of chromate was found higher in chromate resistant isolates compared to the chromate sensitive isolates. Transmission electron microscopy confirmed the accumulation of chromium in cytoplasm in the resistant isolates.     

Effect of Single Species or Mixed Algal (Chlorella vulgaris and Scenedesmus acutus) Diets on the Life Table Demography of Brachionus calyciflorus and Brachionus patulus (Rotifera: Brachionidae)

We tested the effect of two algal diets (Chlorella vulgaris at 1 · 10⁶ cells mL^?1and Scenedesmus acutus at 0.57 · 10⁶ cells mL^?1) offered alone or in combination (50 % each, 75 % or 25 % of either alga), at a total dry weight of 14.2 μg mL^?1on the life table demography of two rotifer species, Brachionus calyciflorus and Brachionus patulus. Data on fecundity indicated that, for any given algal food type or combination of food types, B. calyciflorus had a higher rate of offspring production than B. patulus. Regardless of the rotifer species, the average lifespan varied from 4 to 6 days. The gross and net reproductive rates were higher for B. calyciflorus than for B. patulus. These values ranged from 45 to 71 offspring female^?1 lifespan^?1 and 15 to 24 offspring female^?1 lifespan^?1, respectively, for B. calyciflorus. Generation times (around 4…5 days) were similar for both rotifer species in most of the treatments. Whatever the algal food combination, the rate of population increase (d^?1) of B. patulus was less than half (i.e. 45 %) of that for B. calyciflorus. The observed differences in the life history variables of Brachionus probably explain species‐specific adaptations to the changing phytoplankton composition occurring seasonally in natural waterbodies.