Quantitative aspects of inorganic nutrient fluxes in the Gazi Bay (Kenya): implications for coastal ecosystems

Fluxes of dissolved inorganic nutrients: NH4+, NO2-, NO3-, PO4(3-) and Si(OH)4 from nearshore sediments of Gazi Bay were measured in situ within mangrove, seagrass and coral reef biotopes using benthic flux bell-jar chambers of cross-sectional area 0.066 m2 and volume 0.0132 m3. The objectives were: (1) to determine the influence of benthic fluxes, fluvial discharge and seasonal variations on the nutrient budget in the Bay waters; (2) to determine the effect of tidal and spatial variations on nutrient loads in the water column and (3) to establish the relative importance of the nutrient sources with regard to total community production of the Bay. The directly measured fluxes ranged from -270 to +148 micromol NH4+-N/m2/h; -60 to +63 micromol NO2(-)-N/m2/h; -79 to +41 micromol NO3(-)-N/m2/h; -79 to +75 micromol PO4(3-)-P/m2/h and +30 to +350 micromol Si(OH)4-Si/m2/h for and respectively. It was established that benthic fluxes are the major sources of dissolved inorganic NH4+, NO2- and Si(OH)4 while fluvial sources are important for NO3- and PO4(3-) into Gazi Bay waters. Seasonal variations had an appreciable effect on the PO4(3-) fluxes, N:Si ratio, river nutrient discharge, plankton productivity and important environmental factors such as salinity and temperature. Tidal and spatial variations had no significant effect on nutrient concentrations and net fluxes within the water column. The results imply that benthic fluxes are largely responsible for the nutrient dynamics of the nearshore coastal ecosystems especially where direct terrestrial inputs do not contribute significantly to the nutrient budget.     

Seasonal and spatial dynamics of nutrients and phytoplankton biomass in Victoria Harbour and its vicinity before and after sewage abatement

This study investigated the seasonal and spatial dynamics of nutrients and phytoplankton biomass at 12 stations in Hong Kong (HK) waters during a three year period from 2004 to 2006 after upgraded sewage treatment and compared these results to observations before sewage treatment. Pearl River estuary (PRE) discharge significantly increased NO(3) and SiO(4) concentrations, particularly in western and southern waters when rainfall and river discharge was maximal in summer. Continuous year round discharge of sewage effluent resulted in high NH(4) and PO(4) in Victoria Harbour (VH) and its vicinity. In winter, spring and fall, the water column at all stations was moderately mixed by winds and tidal currents, and phytoplankton biomass was relatively low compared to summer. In summer, the mean surface phytoplankton chl biomass was generally > 9 microL(-1) in most areas as a result of thermohaline stratification, and high nutrients, light, and water temperature. In summer, the potential limiting nutrient is PO(4) in the most productive southern waters and it seldom decreased to limiting levels ( approximately 0.1 microM), suggesting that phytoplankton growth may be only episodically limiting. The mean bottom dissolved oxygen (DO) remained > 3.5 mg L(-1) at most stations, indicating that the eutrophication impact in HK waters was not as severe as expected for such a eutrophic area. After the implementation of chemically enhanced primary sewage treatment in 2001, water quality in VH improved as indicated by a significant decrease in NH(4) and PO(4) and an increase in bottom DO. In contrast, there were an increase in chl a and NO(3), and a significant decrease in bottom DO in southern waters in summer, suggesting that hypoxic events are most likely to occur in this region if phytoplankton biomass and oxygen consumption keep increasing and exceed the buffering capacity of HK waters maintained by monsoon winds, tidal mixing and zooplankton grazing. Therefore, future studies on the long-term changes in nutrient loading from PRE and HK sewage discharge will be crucial for developing future strategies of sewage management in HK waters.     

Impacts of Holocene and modern sea-level changes on estuarine mangroves from northeastern Brazil

Projections of the impacts of modern Relative Sea Level (RSL) rise on estuarine mangroves should be supported by coastal topographic data and records of mangrove dynamics under past RSL change. This work identified inland and seaward mangrove migrations along the Jucuruçu River (Bahia, Northeastern Brazil), during the Holocene based on sedimentary features, palynological and geochemical (δ¹³C, δ¹⁵N, C/N) data integrated with digital elevation models. During the Middle Holocene, in response to RSL rise, the estuary saw mangrove forest establish up to ~37 km inland. RSL stood between -1.4 (+0.36/-2.2 m) and +1 (2.19/0.2 m) around 7400 cal yr BP, and rose to a highest position of +3.25 (4.22/2.45 m) reached around 5350 cal yr BP. That marine incursion caused the inland replacement of freshwater vegetation by mangroves on tidal flats. Since then, the estuary experienced RSL fall, reducing inland tidal water salinity towards the Late Holocene, making that the mangroves were replaced by freshwater floodplain vegetation. Today, in the seaward part of the estuary near its mouth, mangroves occupy an area of ~10 km² along tidal channels. Considering a RSL rise of 98 cm up to the end of the 21^st century, at a rate significantly higher than that of Middle Holocene RSL rise (1.5 mm/yr) and fall (0.6 mm/yr), the current mangrove substrates are expected to drown and/or eroded near the coast, while new mangroves may establish inland, at topographically higher tidal flats in nowadays freshwater-tidal zones. Mangrove area could expand over 13 km² of coastal and flood plain. Following the same interaction between RSL/climate changes and Holocene mangrove dynamics, such upstream mangrove migration may be attenuated or intensified by changes in fluvial discharge. © 2019 John Wiley & Sons, Ltd.     

A method to determine which nutrient is limiting for plant growth in estuarine waters--at any salinity

A method, utilising overlaid graphs for nutrients vs salinity, was developed in order to determine which nutrient is limiting for plant growth in estuarine waters-at any salinity. Dissolved inorganic nitrogen (DIN=NO(3)(-)+NO(2)(-)+NH(4)(+)) and o-phosphate (PO(4)(-)) are the main forms of N and P that are readily bio-available for plant growth in waters and these have a Redfield atomic ratio of N:P=16:1 (i.e. aquatic plants absorb N and P in the average ratio of 16 atoms of N to 1 atom of P). Graphs are prepared for (i) DIN vs salinity and (ii) o-phosphate vs salinity with the vertical scales for DIN and o-phosphate set at a ratio of N:P=16:1; when these graphs are overlaid on each other then the lowermost trendline denotes the limiting nutrient for plant/algal growth-at any salinity. The graphs also indicate the extent by which one or other of the nutrients is limiting--at any salinity. Furthermore, if there is a transition from P to N limitation somewhere along the salinity gradient, then this occurs at the salinity where the trendlines intersect. The concept was applied to three estuaries in the southeast of Ireland and the results show that, in all of these circumstances, P is the limiting nutrient throughout--except for the higher salinities (i.e. salinities 30 per thousand), where either (i) N and P may become equally limiting at salinity approximately 35 per thousand or (ii) N may become limiting at salinity 30 per thousand. Overlaid nutrients vs salinity graphs were also used to demonstrate that, in the estuaries in southeast Ireland, carbon (as dissolved inorganic carbon, DIC=CO(2)+H(2)CO(3)+HCO(3)(-)+CO(3)(2-)) is not the limiting nutrient--at any salinity.     

The sources of the upper and lower halocline water in the Canada Basin derived from isotopic tracers

The Arctic Ocean is almost entirely surrounded by land, with shallow openings to the Pacific through Bering Strait (~ 45 m deep) and to the Atlantic through the Barents Sea (~50—450 m deep) and Fram Strait where the sill depth is around 2500 m. The bathy…     

Dissolved metal contamination in the East River-Long Island sound system: potential biological effects

A suite of dissolved trace metals (Ag, Cd, Cu and Pb), inorganic nutrients (NO(3), PO(4)), and chlorophyll a was measured along a 55 mile transect from the East River into western and central Long Island Sound. The main objectives of this study were to determine the relative levels of contamination from sewage, and to assess its possible biological impact on local waters. The East River-Long Island Sound system receives large volumes of treated sewage and industrial effluent as a result of the heavy urbanization of the area. Despite these strong environmental pressures, this study is among the first to report dissolved metal levels from that region. Consistent with the locations of anthropogenic sources, a strong east-west concentration gradient was observed for Ag, Pb, NO(3) and PO(4) with the highest levels found in the East River. In contrast, dissolved Cd and Cu were relatively constant throughout the area of study, suggesting that sewage sources have a more limited influence on the levels of those metals. Remobilization from contaminated sediments may represent the primary source of Cd and Cu to the Long Island Sound under low-runoff conditions in summer. Chlorophyll a concentrations, used as an indicator of total biomass, were also low in the East River. These low chlorophyll concentrations could not be explained by nutrient or light limitation, water column stratification, or to advection of phytoplankton out of the river during tidal flushing. These preliminary results suggest a potential toxic effect of sewage on the biological communities of the East River.     

Water quality in the Great Barrier Reef region: responses of mangrove, seagrass and macroalgal communities

Marine plants colonise several interconnected ecosystems in the Great Barrier Reef region including tidal wetlands, seagrass meadows and coral reefs. Water quality in some coastal areas is declining from human activities. Losses of mangrove and other tidal wetland communities are mostly the result of reclamation for coastal development of estuaries, e.g. for residential use, port infrastructure or marina development, and result in river bank destabilisation, deterioration of water clarity and loss of key coastal marine habitat. Coastal seagrass meadows are characterized by small ephemeral species. They are disturbed by increased turbidity after extreme flood events, but generally recover. There is no evidence of an overall seagrass decline or expansion. High nutrient and substrate availability and low grazing pressure on nearshore reefs have lead to changed benthic communities with high macroalgal abundance. Conservation and management of GBR macrophytes and their ecosystems is hampered by scarce ecological knowledge across macrophyte community types.     

The characteristics of nutrients and eutrophication in the Pearl River estuary,South China

In the spring of 1998, 24-h time series and synchronization of vertical profiles of NO(3)-N, NO(2)-N, NH(3)-N, PO(4)-P, chlorophyll a, suspended substance, salinity, temperature and other chemical parameters were taken at 10 stations in the Pearl River estuary in order to analyze the status and characteristics of nutrients and eutrophication. The results indicated that dissolved inorganic nitrogen (DIN) mainly came from the four river channels in the main estuary, and NO(3)-N was the main form of DIN in most area. The concentration of DIN was general above 0.30 mg l(-1) in the estuary, and more than 0.50 mgl(-1) in most part. Phosphate from four river channels was not the main sources, but land-based sources from the area near Shenzhen Bay or along the estuary were obvious, and other land-based sources outside the estuary brought by coastal current and flood tide current were also the main contributions. The concentration of phosphate was generally about 0.015 mg l(-1) except the area near Shenzhen Bay. The ratio of N:P was generally high, and it was higher in the north than in the south. The highest ratio was higher than 300, and the lowest one was over 30. The concentration of chlorophyll a was about 0.8-7.8 mg m(-3), and turbidity and phosphate may be the main two limiting factors for algal bloom in the estuary. The concentration of nutrients decreased slightly in the past decade, but still stayed at a high level. The nutrients mainly came from domestic sewage, industrial wastewater, agriculture fertilizer and marine culture in the Pearl River estuary.     

The impact of shrimp farming effluent on bacterial communities in mangrove waters, Ceará, Brazil

The effects of shrimp farm effluents on bacterial communities in mangroves have been infrequently reported. Classic and molecular biology methods were used to survey bacterial communities from four mangroves systems. Water temperature, salinity, pH, total heterotrophic bacteria and maximum probable numbers of Vibrio spp. were investigated. Genetic profiles of bacterial communities were also characterized by polymerase chain reaction (PCR) amplification of eubacterial and Vibrio 16S rDNA using denaturing gradient gel electrophoresis (DGGE). Highest heterotrophic counts were registered in the mangrove not directly polluted by shrimp farming. The Enterobacteriaceae and Chryseomonas luteola dominated the heterotrophic isolates. Vibrio spp. pathogenic to humans and shrimps were identified. Eubacterial genetic profiles suggest a shared community structure independent of mangrove system. Vibrio genetic profiles were mangrove specific. Neither microbial counts nor genetic profiling revealed a significant decrease in species richness associated with shrimp farm effluent. The complex nature of mangrove ecosystems and their microbial communities is discussed.     

PAHs contamination and bacterial communities in mangrove surface sediments of the Jiulong River Estuary, China

Sixteen sediment samples collected from eight transects in a mangrove swamp of the Jiulong River Estuary, Fujian, China were investigated for their content of polycyclic aromatic hydrocarbons (PAHs) and the biodegradation potential of the indigenous microorganisms. The bacterial community structures in the mangrove sediments and in enrichment cultures were also investigated. The results showed that the total PAHs concentration of mangrove sediments ranged from 280 to 1074 ng g(-1) dry weight, that the PAHs composition pattern in the mangrove sediments was dominated by high molecular weight PAH components (4-6 rings), and that Benzo[ghi]perylene and Indeno[1,2,3-cd]pyrene were the most dominant at different stations. Abundant PAH-degrading bacteria were found in all the stations, the values of phenanthrene-degrading bacteria ranged from 5.85 x 10(4) to 7.80 x 10(5) CFU g(-1) dry weight, fluoranthene-degrading bacteria ranged from 5.25 x 10(4) to 5.79 x 10(5) CFU g(-1) dry weight, pyrene-degrading bacteria ranged from 3.10 x 10(4) to 6.97 x 10(5) CFU g(-1) dry weight and the benzo(a)pyrene-degrading bacteria ranged from 5.25 x 10(4) to 7.26 x 10(5) CFU g(-1) dry weight. DGGE analysis of PCR-amplified 16S rDNA gene fragments confirmed that there was a remarkable shift in the composition of the bacterial community due to the addition of the different model PAH compound phenanthrene (three ring PAH), fluoranthene(four ring PAH), pyrene(four ring PAH) and benzo(a)pyrene(five ring PAH) during enrichment batch culture. Eleven strains were obtained with different morphology and different degradation ability. The presence of common bands for microbial species in the cultures and in the native mangrove sediment DNA indicated that these strains could be potential in situ PAH-degraders.     

Effects of environmental stresses on the responses of mangrove plants to spent lubricating oil

The influence of different environmental stresses, including salinity (5-35‰), tidal cycle (6/6, 12/12 and 24/24 h of high/low tidal regimes) and nutrient addition (1-6 times background nitrogen and phosphorus content) on Bruguiera gymnorrhiza and Aegiceras corniculatum grown in sediment contaminated with spent lubricating oil (7.5 L m(-2)) were investigated. The oil-treated 1-year-old mangrove seedlings subject to low (5‰) and high (35‰) salinity had significantly more reduction in growth, more release of superoxide radical (O2·-) and higher activity of superoxide dismutase (SOD) than those subject to moderate salinity (15‰). Extended flooding (24/24 h of high/low tidal regime) enhanced O2·- release and malondialdehyde (MDA) content in both oil-treated species but had little negative effects on biomass production (P>0.05) except the stem of A. corniculatum (P=0.012). The addition of nutrients had no beneficial or even posed harmful effects on the growth and cellular responses of the oil-treated seedlings.     

Multivariate Analysis of Species Distribution: A Survey on Occurence of Mangrove Molluscs in the Bonny and New Calabar Rivers of the Niger Delta Multivariate Analyse von Verbreitungsmustern: Ein Überblick über das Vorkommen von Mangroven-Mollusken im Bonny River und New Calabar River des Niger-Deltas

Studies on distribution and habitats of mangrove molluscs of the Bonny and New Calabar rivers (Niger Delta) were made at ten shore stations from the river mouth to the tidal fresh water zone. Forty-three species were collected and thirty-nine were identified. Investigations into tidal zonation patterns showed that molluscs inhabited the high, mid and low intertidal stretches of the shore. In the tidal fresh water and low-salinity brackish water zone, the low intertidal is poor in molluscs. Many species have restricted salinity ranges occuring either in the high or low salinity limits. The collected data on occurence of species were analyzed by different methods from multivariate data analysis, namely cluster analysis, principal component analysis and partial least squares. These allowed to visualize structures among mollusc species and stations according to distribution patterns and to estimate the degree of relation between these patterns and salinity.     

Contamination and potential biodegradation of polycyclic aromatic hydrocarbons in mangrove sediments of Xiamen, China

Five stations were established in the Fenglin mangrove area of Xiamen, China to determine the concentrations of polycyclic aromatic hydrocarbons (PAHs) and the numbers of PAH-degrading bacteria in surface sediments. Assessing the biodegradation potential of indigenous microorganisms and isolating the high molecule weight (HMW)-PAH degrading bacteria was also one of the aims of this work. The results showed that the total PAH concentration of sediments was 222.59 ng g(-1) dry weight, whereas the HMW-PAH benzo(a)pyrene (BaP) had the highest concentration among 16 individual PAH compounds. The variation in the numbers of PAH-degrading bacteria was 2.62 x 10(2)-5.67 x 10(4)CFU g(-1) dry weight. The addition of PAHs showed a great influence in increasing the microbial activity in mangrove sediments. A bacterial consortium, which could utilize BaP as the sole source of carbon and energy, and which was isolated from mangrove sediments and enriched in liquid medium for nearly one year degraded 32.8% of BaP after 63 days incubation.     

Spatial patterning of water quality in Biscayne Bay, Florida as a function of land use and water management

An objective classification analysis was performed on a water quality data set from 25 sites collected monthly during 1994-2003. The water quality parameters measured included: TN, TON, DIN, NH4+, NO3-, NO2-, TP, SRP, TN:TP ratio, TOC, DO, CHL A, turbidity, salinity and temperature. Based on this spatial analysis, Biscayne Bay was divided into five zones having similar water quality characteristics. A robust nutrient gradient, driven mostly by dissolved inorganic nitrogen, from alongshore to offshore in the main Bay, was a large determinant in the spatial clustering. Two of these zones (Alongshore and Inshore) were heavily influenced by freshwater input from four canals which drain the South Dade agricultural area, Black Point Landfill, and sewage treatment plant. The North Bay zone, with high turbidity, phytoplankton biomass, total phosphorus, and low DO, was affected by runoff from five canals, the Munisport Landfill, and the urban landscape. The South Bay zone, an embayment surrounded by mangrove wetlands with little urban development, was high in dissolved organic constituents but low in inorganic nutrients. The Main Bay was the area most influenced by water exchange with the Atlantic Ocean and showed the lowest nutrient concentrations. The water quality in Biscayne Bay is therefore highly dependent of the land use and influence from the watershed.     

Dredging-induced nutrient release from sediments to the water column in a southeastern saltmarsh tidal creek

Dredging is a large-scale anthropogenic disturbance agent in coastal and estuarine habitats that can profoundly affect water quality. We examined the impact of a small-scale dredging operation in a salt marsh in South Carolina by comparing nutrient levels (NH(4)(+), NO(x), PO(4)(-)) and total suspended solid concentrations before and during dredging activities. Nutrient enrichment was evaluated within the context of tidal, seasonal, and inter-annual variability by using long-term water chemistry data provided by the North Inlet-Winyah Bay National Estuarine Research Reserve. The conditions of the dredging permit (i.e., its relatively small scale), the season chosen for the work (fall-winter), the nature of the sediments dredged (coarse-grained), and the amount of natural variability in the estuary's water chemistry (even on a daily time-scale) all minimized the impact of the dredging activities. Results of this study will add to the limited body of empirical data that should be considered in evaluating future dredging permit applications related to shallow estuarine waterways.     

Effects of spilled oil on microbial communities in a tidal flat

Effects of spilled oil on microbial communities in tidal flats were examined by use of a simulator for a tidal flat ecosystem. The simulator is composed of a wave generator, a tide control device, and a tidal flat. Sediment for the tidal flat was obtained at a natural tidal flat in Hiroshima Bay, Japan. After stabilizing the benthic organisms, fuel oil C was added to the surface of the flat at 1 lm(-2). Although the total number of micro-organisms remained at 1.5-3.5 x 10(9) cells g(-1) dry sediment irrespective of the addition of oil, bacterial communities which were analyzed based on the 16S rDNA showed clear changes after the addition of fuel oil C and after a subsequent recovery period. Bacterial colonies were randomly isolated from the oil-supplemented sediment during the experiments, and the isolates were examined for susceptibility to hydrocarbons in order to screen the oil-susceptible bacteria. The proportion of oil-susceptible bacteria in the isolates decreased with the addition of the oil. Oil-susceptible bacteria showed an inability to assimilate petroleum compounds as well as an inhibition of growth. The possibility of using oil-susceptible bacteria as an indicator of bioremediation in tidal flats was discussed.     

The Gladstone (Australia) oil spill - impacts on intertidal areas: baseline and six months post-spill

In January 2006, 25 tonnes of heavy fuel oil spilled into the Port of Gladstone in Queensland, Australia, from the breached hull of a bulk carrier ship. While approximately 18 tonnes of the oil was recovered, a certain amount of oil was deposited in the intertidal areas of Port Curtis leaving a highly visible, viscous residue. The objectives of this research were to assess the short-term (one month post-spill) and medium-term (six months post-spill) impacts on the intertidal habitat.Sediment polycyclic aromatic hydrocarbons (PAHs) and metal concentrations, mangrove communities and intertidal macroinvertebrates were assessed at oil impacted sites, adjacent sites which were not visibly impacted and reference sites which were located outside the recorded distribution of the oil spill.At one month post-spill, highest PAH concentrations were found at the impacted sites, with concentrations of some PAHs exceeding Australian and New Zealand sediment quality guidelines (SQG) [ANZECC/ARMCANZ, 2000. Sediment Quality Guidelines. Australia and New Zealand Environment and Conservation Council and Agriculture and Resource Management Council of Australia and New Zealand]. However, by six months post-spill PAH concentrations had significantly decreased. PAH concentrations tended to be higher in the back (upper) intertidal zone than at the front of the mangrove stand, and sediment cores indicated that PAH contaminants had remained in the top 4 cm of the sediment. These results indicate that the overall decreased PAH concentrations are likely to be due to evaporation, photoxidation and tidal flushing of the residual oil in these impacted sites.During the initial survey, the impact site contained very few or no crabholes in the high intertidal area, indicating a low crab density in comparison to reference sites. However, at six months post-spill mangrove crab communities appeared to be fully recovered with crabhole densities in impact sites similar to reference sites.While little immediate impact was evident on mangrove parameters, significantly higher seedling mortality and defoliation was apparent at six months post-spill, particularly in the upper intertidal zone. Intertidal macrobenthic communities did not appear to be impacted, either immediately or at six months post-spill. Monitoring of these oil impacted intertidal areas will continue to examine the long-term impacts/recovery from the oil spill.     

The role of urbanization in the flooding and surface water chemistry of Puerto Rico’s mangroves

ABSTRACT

In this study, water level models are constructed to characterize mangrove flooding across urban gradients in Puerto Rico. The most urban sites exhibited 95% longer hydroperiods, 23% lower flood frequencies, and 110% lower depths than the least urban sites. Rainfall importance was explained more by geomorphology and tidal connectivity than by urbanization, but there was evidence for changes in tidal amplitudes along the urban gradient. Relationships between surface water chemical metrics and land cover contradicted previous studies by suggesting lower nutrients and biochemical oxygen demand with increasing urbanization. However, much of this changed with the exclusion of potential outlier sites, as well as under different statistical comparisons. These results reinforce the understanding that the most important drivers of urban mangrove hydrology and water quality in Puerto Rico are likely geomorphology and tidal connectivity, with some influence from surrounding land cover. Results should be considered alongside the reported errors stemming from digital elevation and rainfall response models.     

Microbial Quality in Coastal Waters of Dardanelles in Relation to the Pollution Sources and Transport Pathways

The coastal water quality of Çanakkale Dardanelles (Turkey) was assessed based on bacteriological data and physical–chemical parameters. Total coliform (TC), fecal coliform (FC), and enterococci (ENT) bacteria along with physicochemical parameters were measured monthly up to a year at 12 different sampling sites located through both coastal lines of the strait. The fecal bacteria were abounded at several sampling sites such as B1, B3, G3, and G4 due to direct wastewater discharges and septic leakages or overloading. Storm water runoff and sediment resuspension were important pathways for the transport of coliform and ENT bacteria to the Dardanelles, as the region receives high precipitation and strong winds for most part of the year. The values of pH, temperature, and salinity were in typical ranges for the studied area. The microbiological and physicochemical data were correlated individually and in combination. The best fit correlations for bacterial data were attained between TC and FC or TC and ENT (R = 0.67 or ?0.68), while those for the combined data were obtained for TC with temperature (R = 0.94) and TC with salinity (R = ? 0.70).     

Benthic denitrification and organic matter mineralization in intertidal flats of an enclosed coastal inlet, Ago Bay, Japan

Denitrification (as N(2) flux) and organic matter mineralization (as O(2) uptake) were simultaneously measured in the same set of core sediments from a natural sandy and a constructed muddy tidal flat of Ago Bay, Japan. Denitrification rates at both tidal flats fluctuated between ca. 2-20 micromol N(2)m(-2)h(-1) without showing a clear seasonal pattern, and appeared to be substrate limited as NO(3)(-) enrichment (final concentration ca. 225 microM) caused prompt and similar enhancements of ca. 10-folds. Organic matter mineralization rates were markedly higher at constructed muddy flat compared to those of natural sandy flat, especially in summer, and exhibited pronounced temperature dependent (p<0.01) seasonality for both tidal flats. O(2) uptake rates were generally ca. 2-3 order greater than respective denitrification rates indicating dominance of mineralization processes over N(2) losses.