多鳞鱚Sillago sihama Forskál人工繁殖研究

以天然捕获的多鳞鱚作为亲鱼进行人工繁殖。结果表明:在水温27.5～31.5℃、盐度18～31、pH 8.0～8.5的条件下,经促熟培育自然产卵,10批次共获得受精卵67×104粒,孵出仔鱼42.6×104尾,受精率37.2%～89.3%,孵化率24.8%～97.6%;在水温29～30℃、盐度28、pH 8.4的海水中多鳞鱚胚胎历时14 h 40 min完成整个胚胎发育孵化出膜,经35 d培育出平均全长35 mm的幼鱼约2.8×104尾,育苗成活率达24.6%。     

双棘黄姑鱼的人工繁殖及育苗技术

研究了2002-2003年双棘黄姑鱼(Nibea diacanthus)的人工繁殖、育苗过程。成熟亲鱼注射LRH-A HCG就可产卵，产卵率55．6％～83．3％，受精率71．0％～87．1％，孵化率77．9％～89．3％。苗种培育期间加强水质管理、饵料生物的营养强化和病害防治工作，在水温24．5～29℃、盐度26～32条件下，仔、稚、幼鱼生长迅速。     

双棘黄姑鱼卵巢发育的周年变化

观察了双棘黄姑鱼卵巢发育的周年变化,结果表明,双棘黄姑鱼雌鱼性腺成熟系数(GSI)一年有两个峰值,分别出现在5月份(9.38%±0.32%)和10月份(7.56%±0.43%);HSI与GSI密切相关[R=0.76];卵巢发育水温在19～29℃范围,繁殖水温为24～28℃,在广东省饶平海区,双棘黄姑鱼繁殖时间在每年的5月和10月,属于春、秋二季产卵类型的鱼类。     

引进印度尼西亚大珠母贝人工育苗试验

在雷州半岛海域水温24.0～30.0℃、盐度30.8～32.5、pH8.0～8.3条件下,以牟氏角毛藻,亚心形扁藻,牟氏角毛藻、亚心形扁藻和小球藻,天然混合藻等4种饵料,进行印度尼西亚产大珠母贝亲贝促熟培育试验。结果表明:不同饵料组合在亲贝促熟百分率、排精产卵率和受精率之间差异不显著(P>0.05);阴干温差流水刺激可以有效诱导亲贝排精产卵,优选幼虫和投放合适的采苗器可以提高幼虫的变态率。     

凡纳对虾人工繁殖的初步研究

研究了海水比重的变化对凡纳对虾亲虾人工繁殖活动的影响及换水对亲虾交配率的影响。试验采用剪眼柄、强化培育、控光、换水等技术措施 ,将凡纳对虾培育成熟并使之自然交配产卵 ,孵化出无节幼体 ;亲虾的平均产卵量可达 1 6万粒左右 ,卵的受精孵化率可达 80 %以上。结果表明 :适时换水可将亲虾的交配率从 4 6 %提高到 79% ;凡纳对虾人工繁殖的适宜海水相对密度为 1 0 1 6以上。     

方斑东风螺不同批次罗同质量的比较

2005年5月～8月在雷州市覃斗镇海威水产种苗基地的室内水池进行方斑东风螺亲螺培育．对亲螺不同批次产出的卵囊、卵、及其幼虫进行比较。结果表明：随着亲螺产卵批次的增加，亲螺产卵囊数、卵囊含卵数、产卵量逐次减少；卵囊长、宽逐次变小．厚逐次增加，形状由葵花子片状的比例大变为棒状的比例大；幼虫浮游期逐次延长；变态期幼虫壳长逐次变小；各批次之间的变态率逐次降低；卵径、孵化率、出囊幼虫大小差异不显著。     

不同天然饵料对凡纳滨对虾亲虾性腺发育的影响

分别以双齿围沙蚕(Perinereis aibuhitensis)、杜氏枪乌贼(Loligo duvaucelii)、近江牡蛎(Ostrea riuularis)及三者的混合饵料(1∶1∶1)培育凡纳滨对虾(Litopenaeus vannamei)亲虾至性腺发育成熟,通过分析亲虾性腺发育时间、蜕壳间期、交配率、产卵量、变态率,确定培育亲虾的优质饵料。结果表明:投喂双齿围沙蚕或混合饵料的组与投喂杜氏枪乌贼、近江牡蛎组在产卵量、卵巢发育时间、蜕壳间期、交配率、孵化率、变态率方面具有显著差异(P<0.01)。投喂双齿围沙蚕或混合饵料的亲虾平均产卵量可达21.2×104粒/尾,卵巢平均发育时间为101 h,平均蜕壳间期为218 h,平均交配率可达80%,平均孵化率可达62.99%,平均变态率可达82.44%。表明在凡纳滨对虾亲虾培育的过程中,沙蚕单独或与其他天然饵料混合投喂,较乌贼、牡蛎单独投喂对亲虾性腺发育具有更好的促进效果。     

锯缘青蟹繁殖生物学的研究

为解决锯缘青蟹的种苗生产问题 ,较系统地研究了其繁殖生物学的有关问题。结果表明 ,锯缘青蟹的繁殖季节是 3～ 1 0月 ,体重 1 3 0g以上的青蟹均有交配、生殖能力 ,雌雄比例近 1∶1 ,采用促熟措施可达到雌蟹早产卵的目的 ;适宜胚胎发育水温是 2 0～ 3 0℃ ,当水温 2 5～ 3 0℃时 ,幼体发育随水温增高而加快。并描述了幼体各期的主要特征     

硇洲大黄鱼资源生物学资料分析

1、硇洲——徐闻近海用刺网捕获的大黄鱼主要体长组和主要体重组春汛为351～460毫米和801～1350克,秋汛为381～470毫米和701～1250克。最小体长、体重春汛为275毫米、301克,秋汛为241毫米、251克。最大体长、体重春汛为510毫米、2300克,秋汛为503毫米、2150克。 2、体重与体长的关系式: 春汛 Wt=1.6345×10~(-4)lt~(2.6008) 秋汛 Wt=5.5012×10~(-4)lt~(2.4065) 计算结果表明,同一体长,秋汛的个体比春汛的个体稍重。 3.大黄鱼的生长型适合于Von Bertalanffy生长方程,所求得的各参数值为: 春汛 1~∞=510.67 K=0.3605,t_0=-0.5071。W~∞=2388.41,K=0.2375,t_0=-1.8234。秋汛 1~∞=563.81,K=0.3186,t_0=-0.0623。W~∞=2300.46,K=0.3540,t_0=-0.2642。 4.大黄鱼的生长速度、体长的增长速度以0龄为最大,随着年龄的增大,增长速度逐年减少;体重的增长速度,开始缓慢,随后逐年增大,至增大到最大增长速度之后则逐年减少,拐点年龄春汛为2.80龄、秋汛为2.84龄。 5.年龄组成,春汛为2～5龄,以3、4龄为主,占75％;秋汛为2—6龄,以3、4、5龄居多,占76％。 6.摄食强度,春、秋两汛均以O.I级居多。这可能与产卵期间不食或少食的习性有关。 7.从采样的性腺观察,大部分均为正在产卵的个体,产过卵的也占一定比例。雌雄比,春汛为1:1.52,秋汛为1:0.78。     

中山海域浮式试验鱼礁效果研究

广东省中山市所属海域位于珠江口伶仃洋西侧,属热带和亚热带河口浅海区半咸淡水域,是多种海洋生物种类和多种江河生物种类的产卵场所和幼体成长场所.     

Preliminary study on plasma membrane fluidity of Psychrophilic Yeast Rhodotorula sp.NJ298 in low temperature

The ability of cell to modulate the fluidity of plasma membrane was crucial to the survival of microorganism at low temperature. Plasma membrane proteins, fatty acids and carotenoids profiles of Antarctic psychrophilc yeast Rhodotorula sp. NJ298 were investigated at -3 ℃, 0 ℃ and 8 ℃. The results showed that plasma membrane protein content was greater at -3 ℃ than that at 8 ℃, and a unique membrane polypeptide composition with an apparent molecular mass of 94.7 kDa was newly synthesized with SDS-PAGE analysis; GC analysis showed that the main changes of fatty acids were the percentage of unsaturated fatty acids (C18∶ 1 and C18∶ 2) and shorter chain saturated fatty acid (C10∶ 0) increased along with the decrease of the culture temperature from 8 ℃ to -3 ℃; HPLC analysis indicated that astaxanthin was the major functional carotenoids of the plasma membrane, percentage of which increased from 54.6±1.5% at 8 ℃ to 81.9±2.1% at -3 ℃. However the fluidity of plasma membrane which was determined by measuring fluorescence anisotropy was similar at -3 ℃, 0 ℃ and 8 ℃. Hence these changes in plasma membrane's characteristics were involved in the cellular cold-adaptation by which NJ298 could maintain normal plasma membrane fluidity at near-freezing temperature.     

Information for authors

正1 Aims and Scope Advances in Polar Science(APS)is an international,peer-reviewed journal jointly sponsored by the Polar Research Institute of China and the Chinese Arctic and Antarctic Administration.APS is a comprehensive academic journal dedicated to presentation of multi-disciplinary achievements in Arctic and Antarctic expeditions and research.Its primary purpose     

JOURNAL OF OCEAN UNIVERSITY OF CHINA

<正>Copyright Submfnion of a roanusatpt inks:that the work described has not been published before(exoept In the form of an abstrW or as part of a published lecture,review,or thesis);that it is not under consideration for publication elsewhere that its publication has been approved by ail     

Information for Authors

<正>The Journal of Ocean University of China(Oceanic and Coastal Sea Research)is a comprehensive academic quarterly sponsored by the Ocean University of China,published one volume per year.This journal is devoted to the publication of the theoretical and applied research results on oceanography and marine fisheries.The fieldcovered include the physical,chemical,biological,geo-     

Hydraulics and Hydrology in Mountain Areas

<正>The 35th IAHR World Congress was held in Chengdu,China from September 8 to September 132013.The title of the conference was:"The Wise Finds Pleasure in Water:Meandering through Water Science and Engineering".During the congress,1239 papers were presented in 139sessions for oral presentations and 7 sessions for poster presentations.After the Congress,a Committee was appointed to select some of the best papers presented at the Congress for publication,after a     

Sea ice thickness measurement in spring season in Bothnian Bay using an electromagnetic induction instrument

As an important component of the cryosphere,sea ice is very sensitive to the climate change.The study of the sea ice physics needs accurate sea ice thickness.This paper presents an electromagnetic-induction(EM) technique which can be used to measure the sea ice thickness distribution efficiently,and the successful application in Bothnian Bay.Based on the electromagnetic field theory and the electrical properties of sea ice and seawater,EM technique can detect the distance between the instrument and the ice/water interface accurately,than the sea ice thickness is obtained.Contrastive analysis of the apparent conductivity data obtained by EM and the value of drill-hole at same positions allows a construction of a transformable formula of the apparent conductivity to sea ice thickness.The verification of the sea ice thickness calculated by this formula indicates that EM technique is able to get reliable sea ice thickness with average relative error of only 12%.The statistic of all ice thickness profiles shows that the level ice distribution in Bothnian Bay was 0.4-0.6 m.     

Thermohaline structure inhomogeneity associated with polynia at the northern margin of Emery Ice-shelf

Based on the hydrographic data in austral summer during the 22nd Antarctic Expedition of China(2005/2006),some features can be found about the northern margin of Emery ice shelf as follows.The heat content in the surface layer(0-50 m) at the eastern end and the western end of the ice-shelf margin is much higher than that at the middle.The upper mixing-layer depth and the seasonal thermocline depth at the middle of the ice-shelf northern margin are much shallower than those at the both ends.However there is much less difference between the middle and the ends in the bottom layer.The remote sensing photos show that the inhomogeneity in the surface-layer water is closely related to the spatial distribution of the floes and polynia in the area.     

Phylogenetic analysis of cultivable bacteria isolated from Arctic sea-ice

Phylogenetic analysis based on 16S rDNA of 8 strains of cultivable bacteria isolated from Arctic sea-ice was studied.The results showed that strain BJ1 belonged to genus Planococcus,which was a genus of low mole percent G C gram-positive bacteria;strain BJ6 belonged to genus Burkholderia of β-proteobacteria and the rest 6 strain all belonged to γ-proteobacteria,of which strain BJ8 was a species of Pseudoalteromonas,strain BJ2-BJ5 and BJ7 were members of genus Psychrobacter.Phylogenetic analysis also indicated that bacteria of genus Psychrobacter of the isolates formed a relatively independent phylogenetic cluster in comparison with other bacteria belonged to genus Psychrobacter.     

Information for authors

正1 Aims and Scope Advances in Polar Science(APS)is an international,peer-reviewed journal jointly sponsored by the Polar Research Institute of China and the Chinese Arctic and Antarctic Administration.APS is a comprchensive academic journal dedicated to presentation of multi-disciplinary achievements in Arctic and Antarctic expeditions and research.Its primary purpose is to publish achievements in fundamental research,applied