Vergleichende Untersuchungen über das Phosphataufnahmevermögen ausgewählter Bakterienarten

The P-uptake by Acinetobacter calcoaceticus, Pseudomonas aeruginosa and Escherichia coli is determined in batch culture with peptone/glucose/sodium chloride or peptone/acetate/sodium chloride as substrate at 60 to 120 mg/1 orthophosphate for 20 h with cell densities of 2 · 10⁷/ml (Acinetobacter) or 1.2 · 10⁹. The measurements were carried out by means of an Na₂HPO₄ (³²P) addition of 95 to 420 kBq. During the stationary phase the bacteria achieved the following P-contents in the biomass in fg/g bacterium: Acinetobacter 6 to 13, Pseudomonas 0.2 to 0.6, Escherichia 0.04 to 0.09; during the phase of growth Acinetobacter achieved 40 to 100 fg/bacterium. Acetate as the substrate did not result in any increase of the P-uptake. The maximum accumulation with Acinetobacter was 13 % P in the dry substance.     

The Carbonate Radical (HCO3·/CO3–·) as a Reactive Intermediate in Water Chemistry: Kinetics and Modelling

Results from kinetic laboratory studies of reactions of the carbonate radical anion (CO₃^–·) with aromatic compounds in aqueous solution at T = 298 K are presented. Data were obtained in using a laser photolysis laser long-path absorption (LP-LPLA) apparatus which was designed for direct time-resolved studies of radical reactions. For the reactions of CO₃^–· with hydroquinone dimethyl ether (2), methyl anisole (3), benzene (4), p-xylene (5), toluene (6), chlorobenzene (7), nitrobenzene (8), and benzonitrile (9), rate coefficients of k₂ = (3.0 ± 0.6)·10⁷ M^–1 s^–1, k₃ = (9.7 ± 1.7)·10⁵ M^–1 s^–1, k₄ = (3.2 ± 0.7)·10⁵ M^–1 s^–1, k₅ = (3.8 ± 0.9)·10⁴ M^–1 s^–1, k₆ = (6.8 ± 2.3)·10⁴ M^–1 s^–1, k₇ = (2.7 ± 0.6)·10⁵ M^–1 s^–1, k₈ = (1.4 ± 0.5)·10⁴ M^–1 s^–1, and k₉ < 1.3·10² M^–1 s^–1 were obtained. In further studies the effect of temperature on the reactions (2), (4), and (5) has been studied. The kinetic data obtained for the reaction of the carbonate radical anion with aromatic compounds were compared to the corresponding reactions of the hydroxyl radical. Finally, these kinetic data were used within a simple model system to investigate the implications of carbonate radical anion kinetics within water treatment processes. It is shown that the degradation of organic pollutants in ^·OH-radical based water treatment may proceed via the CO₃^–·/HCO_3· radical under certain conditions.     

Biodegradation of Crude Oil by Individual Bacterial Strains and a Mixed Bacterial Consortium Isolated from Hydrocarbon Contaminated Areas

A preliminary study was undertaken to determine the optimal conditions for the biodegradation of a crude oil. Among 57 oil‐degrading bacterial cultures isolated from oil‐contaminated soil samples, Bacillus sp. IOS1‐7, Corynebacterium sp. BPS2‐6, Pseudomonas sp. HPS2‐5, and Pseudomonas sp. BPS1‐8 were selected for the study based on the efficiency of crude oil utilization. Along with the selected individual strains, a mixed bacterial consortium prepared using the above strains was also used for degradation studies. The mixed bacterial consortium showed more growth and degradation than did individual strains. At 1% crude oil concentration, the mixed bacterial consortium degraded a maximum of 77% of the crude oil. This was followed by 69% by Pseudomonas sp. BPS1‐8, 64% by Bacillus sp. IOS1‐7, 45% by Pseudomonas sp. HPS2‐5, and 41% by Corynebacterium sp. BPS2‐6. The percentage of degradation by the mixed bacterial consortium decreased from 77 to 45% as the concentration of crude oil was increased from 1 to 12%. Temperature of 35°C and pH 7 were found to be optimum for maximum degradation.     

Products and Kinetics of the OH‐radical‐induced Dealkylation of Atrazine

Hydroxyl radicals, generated radiolytically in N₂O/O₂‐saturated solutions, yield in their reaction with atrazine equal amounts of deethylatrazine and acetaldehyde (40% of OH radical yield) and deisopropylatrazine and acetone (16%), respectively. The precursors of deethylatrazine and acetaldehyde is their Schiff base which hydrolyzes slowly (OH^–‐catalyzed: k = 5.2 dm³ mol^–1 s^–1). The hydrolysis of the Schiff base of deisopropylatrazine and acetone is too fast to be detected. In a pulse radiolysis experiment, the intermediate formed upon OH‐radical attack (k = 3·10⁹ dm³ mol^–1 s^–1) has a strong absorption at 440 nm. It decays in the presence of oxygen (k = 1.3·10⁹ dm³ mol^–1 s^–1), and upon deprotonation [pK_a(peroxyl radicals) ≈ 10.5] the peroxyl radicals thus‐formed eliminate superoxide radicals (k = 2.9·10⁵ s^–1). s‐Triazine itself reacts much more slowly with OH radicals (k = 9.7·10⁷ dm³ mol^–1 s^–1). This can explain, why in the case of atrazine in comparison to other aromatic compounds, e.g. toluene, the addition of the OH radical to the ring (estimated at ca. 40%) is of relatively little importance as compared to an H‐abstraction from (activated) positions of the side groups.     

The Impact of Biofilm Growth on Transport of Escherichia coli O157:H7 in Sand

Understanding the transport behavior, survival, and persistence of pathogens such as Escherichia coli O157:H7 in the subsurface is essential to protection of public health. In this study, the transport of E. coli O157:H7 in a two‐dimensional bench‐scale sand aquifer system, hereafter referred to as the sandbox, was investigated, with a focus on the impact of biofilm development on E. coli retention and survival. Biofilm growth was initiated through flushing with unsterilized groundwater and addition of glucose, nitrate, and phosphate. Retention of E. coli from an injection test in clean sand, prior to promotion of biofilm growth, was approximately 9%. Subsequent to biofilm growth, 47% of injected E. coli cells were retained under similar flow conditions. After 10 d of no flow, sterile water was flushed through the biofouled sandbox and substantial concentrations (up to 1.5 × 10⁵ cells/mL) of E. coli were measured in the effluent indicating that E. coli had survived the starvation period. Confocal laser scanning microscopy revealed that E. coli were located not only on the surface but also within the biofilm. Imposition of starvation conditions resulted in biofilm sloughing and possible mobilization of biofilm‐associated E. coli.     

Erosion of a cyclic saltmarsh in Morecambe Bay,North-West England

This paper presents results of investigations (1983–1992) into rates of change, morphology and processes occurring during the current erosional phase in a Morecambe Bay cyclic saltmarsh, in which it has narrowed from c. 1000 m (1975) to c. 150 m (1992). Monthly monitoring of marsh edge erosion and creek knickpoint retreat has revealed temporal and spatial variations. Highest frequency changes of low magnitude coincided with non-storm conditions and overmarsh tides above 5·80 m OD, which submerged the whole marsh. Less frequent changes of greater magnitude were associated with both overmarsh tides and strong onshore winds over 15 ms^?1, which generated high energy waves. The lowest frequency change of greatest magnitude occurred during an extreme onshore storm event and surge. Morphologically, during the erosional phase, a low angled landward slope was generated as erosion of the c. 0·5 m high active seaward cliff coincided with vertical accretion of 0·07 ma^?1 of relatively coarse sediment on the marsh surface immediately landward. Tidal hydrodynamics strongly influence the saltmarsh, which is confined to the upper 2·5 m of the macrotidal range (maximum c. 10·5 m). During overmarsh spring tides (maximum creek flood flow rate 0·13 ms^?1, up to bankfull level), flooding begins over lower landward creek banks before submerging the higher marsh edge. During ebb tides, water trapped by this higher edge can escape seaward only via the creeks (maximum ebb velocities 2·07 ms^?1 below bankfull level). Wave erosion also is limited to spring tides. Monthly mapping of the Kent Estuary channel pattern seaward of the saltmarsh showed that medium term higher erosion rates were related to the presence of a large channel, which lowered the adjacent creek base level and allowed larger waves to attack the marsh edge than when a sandbank flanked the marsh. Major River Kent channel shifts appear to initiate accretional or erosional phases of cyclic saltmarsh development.     

Zur ökonomischen Bedeutung der biologischen Selbstreinigung

In twelve flowing water (Q 1.04… 30.4 m³/s, 0.5… 1.2 m/s) the self-purification efficiency in 110 river sections (L 1.3… 26.4 km) is determined as the load difference in kg · d^?1 COD-Mn as well as in g · m^?3 referred to the daily passage. In the economical comparison, the self-purification efficiency is valued as substitution for wastewater treatment plants of the same capacity. At a mean specific capacity of 8.6 g · m^?3, the self-purification efficiency of the investigated waters is equivalent to economical values of 124 · 10³… 534 · 10³ M km^?1 investment costs, 11 · 10³… 80 · 10³ M · a^?1km^?1 operating costs and 5… 81 MWh · a^?1 km^?1 expenditure of energy. The specific capacity in g · m^?3 COD-Mn shows an exponential regression to the degree of saprobity (L = 0.015 · exp (1.7358 · S)). From this empirical model the limits of the self-purification capacity of aerobic waters by oxygen input can be detected: in respective examples more than 50% of the required oxygen input are due to weirs.     

Similarities between strike-slip faults at different scales and a simple age determining method for active faults

Abstract Several differently scaled strike‐slip faults were examined. The faults shared many geometric features, such as secondary fractures and linkage structures (damage zones). Differences in fault style were not related to specific scale ranges. However, it was recognized that differences in style may occur in different tectonic settings (e.g. dilational/contractional relays or wall/linkage/tip zones), different locations along the master fault or different fault evolution stages. Fractal dimensions were compared for two faults (Gozo and San Andreas), which supports the idea of self‐similarity. Fractal dimensions for traces of faults and fractures of damage zones were higher (D ～1.35) than for the main fault traces (D ～1.005) because of increased complexity due to secondary faults and fractures. Based on the statistical analysis of another fault evolution study, single event movements in earthquake faults typically have a maximum earthquake slip : rupture length ratio of approximately 10^?4, although this has only been established for large earthquake faults because of limited data. Most geological faults have a much higher maximum cumulative displacement : fault length ratio; that is, approximately 10^?2 to 10^?1 (e.g. Gozo, ～10^?2; San Andreas, ～10^?1). The final cumulative displacement on a fault is produced by accumulation of slip along ruptures. Hence, using the available information from earthquake faults, such as earthquake slip, recurrence interval, maximum cumulative displacement and fault length, the approximate age of active faults can be estimated. The lower limit of estimated active fault age is expressed with maximum cumulative displacement, earthquake slip and recurrence interval as T ? (d_max /u) · I(M).     

Energy balance and evaporation loss of an agricultural reservoir in a semi‐arid climate (south‐eastern Spain)

A typical agricultural water reservoir (AWR) of 2400 m² area and 5 m depth, located in a semi‐arid area (southern Spain), was surveyed on a daily basis for 1 year. The annual evaporation flux was 102·7 W m^?2, equivalent to an evaporated water depth of 1310 mm year^?1. The heat storage rate G exhibited a clear annual cycle with a peak gain in April (G ～ 45 W m^?2) and a peak loss in November (G ～ 40 W m^?2), leading to a marked annual hysteretic trend when evaporation (λE) was related to net radiation (R_n). λE was strongly correlated with the available energy A, representing 91% of the annual AWR energy loss. The sensible heat flux H accounted for the remaining 9%, leading to an annual Bowen ratio in the order of 0·10. The equilibrium and advective evaporation terms of the Penman formula represented 76 and 24%, respectively, of the total evaporation, corresponding to a annual value of the Priestley–Taylor (P–T) coefficient (α) of 1·32. The P–T coefficient presented a clear seasonal pattern, with a minimum of 1·23 (July) and a maximum of 1·65 (December), indicating that, during periods of limited available energy, AWR evaporation increased above the potential evaporation as a result of the advection process. Overall, the results stressed that accurate prediction of monthly evaporation by means of the P–T formula requires accounting for both the annual cycle of storage and the advective component. Some alternative approaches to estimating R_n, G and α are proposed and discussed. Copyright © 2009 John Wiley & Sons, Ltd.     

Processes controlling rapid temperature variations on rock surfaces

In arid environments, thermal oscillations are an important source of rock weathering. Measurements of temperature have been made on the surface of rocks in a desert environment at a sampling interval of 0·375 s, with simultaneous measurements of wind speed, air temperature, and incoming shortwave radiation. Over timescales of hours, the temperature of the rock surface was determined primarily by shortwave radiation and air temperature, while rapid temperature variations, high dT/dt, at intervals of seconds or less, were determined by wind speed. The maximum values of temperature change and time spent above 2°C min^?1 increased at high measurement rates and were much higher than previously reported. The maximum recorded value of dT/dt was 137°C min^?1 and the average percentage time spent above 2°C min^?1 was ～70 ± 13%. Maximum values of dT/dt did not correlate with the maximum values of time spent above 2°C min^?1. Simultaneous measurements of two thermocouples 5·5 cm apart on a single rock surface had similar temperature and dT/dt values, but were not correlated at sampling intervals of less than 10 s. It is suggested that this is resulting from rapid fluctuations due to small spatial and timescale wind effects that are averaged out when data is taken at longer sampling intervals, ～10 s or greater. Published in 2010 by John Wiley & Sons, Ltd.     

Untersuchungen zum Vorkommen von R+-Bakterien im Flußwasser

With colony numbers of 2 · 10⁴… 3 · 10⁵ and coliform contents of 2 · 10³… 1 · 1 · 10⁴ per ml, in the investigated river section 2.1% of colonies were resistant to tetracycline, 0.3% to chloramphenicol, 7.5% to kanamycin, 5.4% to trimethoprim, 12.7% to ampicillin and 0.14% to gentamycin. For the coliforms the resistances were 5.1% to tetracycline, 2.1% to chloramphenicol, 2.7% to kanamycin and 0.15% to gentamycin. There were isolated 63 different resistance types. Of the resistant Klebsiella strains 75% were resistant to three or more antibiotics. Of the coliform bacteria (Escherichia coli, Klebsiella, Enterobacter, Citrobacter) 31.6… 51.9% of the strains transferred their antibiotics-resistance to Escherichia coli K-12, for the antibiotics-resistant strains of Pseudomonas and Aeromonas a transfer of resistance was not observed at all.     

Large roughness element effects on sand transport,Oceano Dunes,California

The effect of large roughness elements on sand transport efficiency was evaluated on a coastal sand sheet by measuring sand flux with two types of sand traps [Big Spring Number Eight (BSNE) and the Cox Sand Catcher (CSC)] at 30 positions through a 100 m‐long × 50 m‐wide roughness array comprised of 210 elements each with the dimensions 1·17 m long × 0·4 m high × 0·6 m wide. The 210 elements were used to create a roughness density (λ) of 0·022 (λ = n bh/S, where n is the number of elements, b the element breadth, h the element height, and S is the area of the surface that contains all the elements) in an area of 5000 m². The mean normalized saltation flux (NSF) values (NSF = outgoing sand flux/incoming sand flux) at the furthest downwind distance for the two trap types were 0·44 and 0·41, respectively. This is in excellent agreement with an empirical model prediction of 0·5. The reduction in saltation flux is similar to an earlier separate study for an equivalent λ composed of elements of similar height (0·36 m), even though the roughness element forms were different (rectangular in this study as opposed to circular) as were the horizontal porosity of the arrays (49% versus 16%). This corroborates earlier results that roughness element height is a critical parameter that enhances reduction in sand transport by wind for similar λ configurations. The available data suggest the form of the relationship between transport reduction efficiency and height is likely a power relationship with two limiting conditions: (1) for elements ≤ 0·1 m high the effect is minimized, and (2) as element height matches and then exceeds the maximum height of the saltation layer (≥ 1 m), the effect will stabilize near a maximum of NSF ≈ 0·32. Copyright © 2012 John Wiley & Sons, Ltd.     

The sanitary state of Pomeranian Bay and Gulf of Gdańsk waters during the flood of 1997

Summary Due to the 1997 summer flood, the Pomeranian and Gdańsk Bays were polluted with a significant amount of bacteriologically contaminated Oder and Vistula rivers waters. The purpose of this study was to determine the sanitary state of both bays during the flood and directly after it, the range of direction of distribution of the bacteriological contamination brought in by Vistula and Oder waters. On the basis of examination results, the sanitary state of the bays waters was found to be worse, particularly with regard to indicators: total bacterial number (at 20 °C and 37 °C) and Most Probable Number (MPN) of coliform and faecal coliform bacteria. Bacteria of the Pseudomonas group(Pseudomonas aeruginosa, Pseudomonas fluorescens), Enterobacteriaceae group (m.in.Proteus vulgaris), as well as enterococci(Streptoccus) and staphylococci(Staphylococcus) were found in water samples from Pomeranian and Gdańsk Bays. In the Pomeranian Bay the waters spread to the west (due to weather conditions), whereas in Gdańsk Bay they remained mainly in the coastal zone and then moved to the east. An improvement in the sanitary state of both bay waters was observed at the end of August. Zusammenfassung W?hrend des Sommer-Hochwassers 1997 wurden die Gew?sser der Pommerschen und der Danziger Bucht mit kontaminiertem Wasser aus den Flüssen Oder und Weichsel verunreinigt. Ziel dieser Arbeit ist es, den Grad der bakteriellen Verunreinigungen durch einstr?mendes Flu?wasser aus Oder und Weichsel in die Pommersche und die Danziger Bucht w?hrend des Hochwassers und direkt danach zu bestimmen. Auf der Basis von Messungen wurde eine Vermehrung von Colibakterien im Wasser beider Buchten unter Berücksichtigung folgender Indikatoren festgestellt: 1. Gesamtzahl an Bakterien (Wassertemperatur 20 °C and 37 °C) und 2. vermuteter Anteil von gesamtcoliformen und f?kalcoliformen Bakterien. Die Wasserproben aus der Danziger und der Pommerschen Bucht enthielten sowohl F?ulnisbakterien(Pseudomonas aeruginosa, Pseudomonas fluorescens) als auch Bakterien aus der Gruppe der Enterobacteriaceae(Proteus vulgaris), Staphylokokken(Staphylococcus) und F?kalstreptokokken(Streptococcus). Infolge Wetterver?nderung verlagerte sich das kontaminierte Wasser aus der Pommerschen Bucht nach Westen. In der Danziger Bucht hingegen blieb das kontaminierte Wasser zun?chst im Küstenbereich und verlagerte sich dann ostw?rts. Ende August wurde eine Verbesserung der Wasserqualit?t in beiden Buchten ermittelt.     

GIS‐assisted modelling for debris flow hazard assessment based on the events of May 1998 in the area of Sarno,Southern Italy: Part I. Maximum run‐out

Based on the debris flow events that occurred in May 1998 in the area of Sarno, Southern Italy, this paper presents an approach to simulate debris flow maximum run‐out. On the basis of the flow source areas and an average thickness of 1·2 m of the scarps, we estimated debris flow volumes of the order of 10⁴ and 10⁵ m³. Flow mobility ratios (ΔH/L) derived from the x, y, z coordinates of the lower‐most limit of the source areas (i.e. apex of the alluvial fan) and the distal limit of the flows ranged between 0·27 and 0·09. We performed regression analyses that showed a good correlation between the estimated flow volumes and mobility ratios. This paper presents a methodology for predicting maximum run‐out of future debris flow events, based on the developed empirical relationship. We implemented the equation that resulted from the calibration as a set of GIS macros written in Visual Basic for Applications (VBA) and running within ArcGIS. We carried out sensitivity analyses and observed that hazard mapping with this methodology should attempt to delineate hazard zones with a minimum horizontal resolution of 0·4 km. The developed procedure enables the rapid delineation of debris flow maximum extent within reasonable levels of uncertainty, it incorporates sensitivities and it facilitates hazard assessments via graphic user interfaces and with modest computing resources. Copyright © 2007 John Wiley & Sons, Ltd.     

Quantifying periglacial erosion: insights on a glacial sediment budget,Matanuska Glacier,Alaska

Glacial erosion rates are estimated to be among the highest in the world. Few studies have attempted, however, to quantify the flux of sediment from the periglacial landscape to a glacier. Here, erosion rates from the nonglacial landscape above the Matanuska Glacier, Alaska are presented and compare with an 8‐yr record of proglacial suspended sediment yield. Non‐glacial lowering rates range from 1·8 ± 0·5 mm yr^?1 to 8·5 ± 3·4 mm yr^?1 from estimates of rock fall and debris‐flow fan volumes. An average erosion rate of 0·08 ± 0·04 mm yr^?1 from eight convex‐up ridge crests was determined using in situ produced cosmogenic ¹⁰Be. Extrapolating these rates, based on landscape morphometry, to the Matanuska basin (58% ice‐cover), it was found that nonglacial processes account for an annual sediment flux of 2·3 ± 1·0 × 10⁶ t. Suspended sediment data for 8 years and an assumed bedload to estimate the annual sediment yield at the Matanuska terminus to be 2·9 ± 1·0 × 10⁶ t, corresponding to an erosion rate of 1·8 ± 0·6 mm yr^?1: nonglacial sources therefore account for 80 ± 45% of the proglacial yield. A similar set of analyses were used for a small tributary sub‐basin (32% ice‐cover) to determine an erosion rate of 12·1 ± 6·9 mm yr^?1, based on proglacial sediment yield, with the nonglacial sediment flux equal to 10 ± 7% of the proglacial yield. It is suggested that erosion rates by nonglacial processes are similar to inferred subglacial rates, such that the ice‐free regions of a glaciated landscape contribute significantly to the glacial sediment budget. The similar magnitude of nonglacial and glacial rates implies that partially glaciated landscapes will respond rapidly to changes in climate and base level through a rapid nonglacial response to glacially driven incision. Copyright © 2009 John Wiley & Sons, Ltd.     

Ratio of neonate to adult size explains life history characteristics in cladoceran zooplankton

The size ratio of adults to neonates varies greatly in cladocerans across taxa. In this study we tested the impact of this ratio (using length basis) on the selected life history parameters of nine species of freshwater cladocerans (Alona rectangula, Pleuroxus aduncus, Scapholeberis mucronata, Moina macrocopa, Moina wierzejskii, Ceriodaphnia dubia, Daphnia laevis, Diaphanosoma birgei and Daphnia pulex). Experiments were conducted on a mixed diet of Chlorella vulgaris (1.0·10⁶ cells mL^–1) and Scenedesmus acutus (0.5·10⁶ cells mL^–1) at (22 ± 1)°C. Using a cohort life table experimental design, we quantified age‐specific reproductive output. Body sizes of neonates and adults as well as dry weight were determined using standard procedures. We found that the ratio of neonate to adult body length decreased with increasing body size of the adult. In terms of clutch size, however, smaller taxa produced lower numbers of offspring than larger ones. The influence of ratio neonate/adult size on average lifespan, age at first reproduction and at the maximum clutch showed U‐shaped curves. In smaller taxa (< 700 μm) and larger taxa (> 1500 μm) the ratio between the neonate and adult body size was less than 0.4 or more than 0.6. In medium sized taxa (1000?1500 μm), the ratio of neonate to adult size was between 0.4 and 0.6. The results have been discussed in relation to the life history strategies of cladocerans.     

Sediment-transport competence of rain-impacted interrill overland flow

Laboratory experiments to determine the maximum size of sediment transported in shallow, rain-impacted flow were conducted in a recirculating flume 4·80 m long and 0·50 m wide. Rainfall intensities were varied between 51 and 138 mm h⁻¹, flow was introduced from a header tank into the flume at rates ranging from 0 to 0·64 l s⁻¹, and experiments were conducted on gradients between 3·5 and 10°. The following equation was developed: ML = (REFE)^1·6363 in which M is particle mass, L is distance moved in unit time (cm min⁻¹), RE is rainfall energy (J m⁻² s⁻¹) and FE is flow energy (J m⁻² s⁻¹). This equation can be used to predict sediment-transport competence of interrill overland flow. The equation is limited in its utility insofar as it has been developed using quartz grains and takes no account of variations in absorption of rain energy by natural ground surfaces. © 1998 John Wiley & Sons, Ltd.     

Excrétion de carbone organique par le phytoplancton lémanique

The release of soluble organic carbon by the phytoplankton in the trophogenic layer of Lake Geneva has been investigated by¹⁴C method from March to November 1984. The carbon excretion rate varied from 0.01 to 0.5 mg C·m⁻³·h⁻¹ and depended primarily upon the rate of its photosynthetic assimilation. The carbon excretion rate varied generally between 2 and 5% of photoassimilated carbon and exceptionally exceeded 6%. These values were underestimated from part of heterotrophic reassimilation. The study of the carbon balance over 24 hours has shown that night excretion represents less than 10% of losses due to respiration. Heterotrophic activity measured by¹⁴C glucose assimilation exhibited seasonal variations following the same general pattern as excretion. The quantity of phytoplankton excretion products appeared to be superior to the needs in organic compounds of heterotrophic organisms in the trophogenic layer.      

Untersuchungen über die Beeinflussung der planktischen Photosynthese durch anorganische Metallsalze im eutrophen Alpnachersee und der mesotrophen Horwer Bucht

The effects on phytoplankton photosynthesis of inorganic metal salts HgCl₂, CuSO₄, Cd(NO₃)₂, ZnCl₂ and Pb(NO₃)₂ were studied over monthly intervals. In experiments with individual metals, phytoplankton photosynthesis was not adversely affected if the concentration increase above background levels did not exceed 10^?9 mole Hg/l, 5·10^?9 mole Cu/l, 2·10^?8 mole Cd/l, 5·10^?8 mole Zn/l and 2·10^?7 mole Pb/l, respectively. However when the concentration was increased by 5·10^?10 mole Hg/l+5·10^?9 mole Cu/l+5·10^?9 mole Cd/l+5·10^?8 mole Zn/l+5·10^?8 mole Pb/l photosynthesis was significantly reduced, due to a synergetic effect of the combined metals. The influence of phytoplankton density, pH-value concentration of calcium, dissolved organic nitrogen and allochthonous debris on heavy metal toxicity was investigated. Changes in phytoplankton composition are believed to the main reason for the seasonal variation in the toxic effects of heavy metals.