养殖美洲鳗鲡的主要疾病与防治

本文通过对养殖美洲鳗鲡疾病的调查,分别介绍了１１种疾病的病原、流行病学、疾病特征及防治措施。     

Fish community responses to invasive fish removal and installation of an exclusion barrier at Lake Ohinewai,Waikato

Introduced common carp (Cyprinus carpio) are a recognised threat to New Zealand’s freshwater ecosystems. In 2011, an invasive fish removal programme was undertaken in 16.8 ha Lake Ohinewai, a Waikato riverine lake. Active fish removal using netting and boat electrofishing was supported by installation of a one-way exclusion barrier on the outflow to Lake Ohinewai. The barrier was designed to allow fish passage downstream out of the lake, while preventing adult carp from moving upstream. The estimated carp population was reduced from 8,548 fish (5,863–12,937, 95% confidence limits) in 2011–454 fish (251–889, 95% CL) in 2014. However, by 2016 the carp population had recovered to 2,063 fish (1,070–4,328, 95% CL) with increased abundance in invasive brown bullhead catfish (Ameiurus nebulosus) also observed. This research demonstrates that carp populations can be reduced to low abundances in the short to medium term, but continued removal will be required for on-going control.     

The influence of environmental cues on the activity of silver eels (Anguilla dieffenbachii) in Lake Manapouri,New Zealand

Lake Manapouri in the South Island of New Zealand has a significant hydroelectric generation facility, which potentially has a negative impact on the successful emigration of longfin eels (Anguilla dieffenbachii) from the upper Waiau catchment. Consequently, a trap-and-transfer programme has been implemented to allow silver (migratory) eels access to the sea. The present study analysed data on the timing of captures and numbers of silver eels over four seasons, including the influence of possible migration cues. Silver eels were captured over an extended season of seven months (November–May), with peak numbers during December and January. Flow at the lake outflow was found to have a weak relationship with the number of eels caught, as did lake level, but moon phase and rainfall had no significant effect. These results highlight the variability in silver longfin eel behaviour both in terms of timing and response to potential environmental cues.     

Effect of size grading on the growth performance of shortfin eel (Anguilla australis) during its yellow stage

It is generally believed that size grading in a fish farm improves the total biomass output, because it will minimise the stress imposed by larger individuals over small individuals, resulting in higher feed intake and growth rate. The objective of this study was to evaluate the effect of size grading on individual growth performance of yellow New Zealand shortfin eel (Anguilla australis). A 109 day trial was conducted in a recirculation aquaculture system (RAS) with 103 eels of similar initial weight (101 ± 12 g). All eels were individually marked by PIT (passive integrated transponder) tags. On day 42, eels were graded to establish the following groups: only small eels (S-graded ≤ 135 g), only large eels (L-graded > 135 g), and small and large eels together (S-ungraded and L-ungraded). The large eels showed better specific growth rate (SGR), feed intake (FI) and feed conversion ratio (FCR) than the small eels. Nevertheless, no significant differences were found either between L-graded and L-ungraded or between S-graded and S-ungraded. The age of eels was not significantly different between groups. These findings suggest that the individual growth rate performance of the eels is not primarily a consequence of social interaction among tank-mates.     

欧洲鳗鲡胃肠道激素免疫组织化学的研究

用免疫组织化学技术研究了9种哺乳动物胃肠道激素抗体在正常生长欧鳗和僵鳗胃肠道的定位分布,研究结果表明,5－HT,SOM,GAS,α－1－AT和P物质免疫活性内分泌细胞存在于正常鳗和僵鳗胃肠道中,其余4种抗体显阴性反应,文中讨论了胃肠道激素在僵鳗成因中可能的生理作用。     

欧洲鳗鲡的性分化和僵鳗成因的探讨

欧洲鳗鲡性分化时间在白仔鳗转变为黑仔鳗时期体长１０．５－１２．０ｃｍ,性腺切片观察可见雌性卵巢中有原始卵原细胞雄性生精小管中有精原细胞,性别分化明显旱鳗鲡从福建省两个养鳗场采集僵鳗,取甚生腺切片观察有精原除个别僵鳗外,全部为雄性,提示僵鳗成因可能与性别有关。文中讨论了发生僵鳗的复杂因素。     

Trace metals in Japanese eel Anguilla japonica in relation to ecological migratory types and growth stages

In order to understand the metal concentrations in Japanese eel Anguilla japonica, nine elements were analyzed in the livers of different migratory types of eels collected from Tokushima region (south Japan). Migratory types were defined by examining the Sr:Ca ratio in otoliths. The results showed that there were significant differences in V, Cr, Cd, and Pb concentrations among the migratory types. Mature-sea-eels show a higher risk of metal accumulation than other migratory types of eels, and the concentrations of Mn, Cu, and Zn in mature eels were significantly higher than those in immature eels. The study suggests that the eel liver is a valuable bioindicator for trace metals; however, when using the eel as a bioindicator to reveal the pollutants in aquatic systems, life history analysis should be carried out for accurate interpretation of the results.     

鳗鲡目鱼类线粒体蛋白质编码基因易位及系统演化关系分析

鳗鲡目鱼类线粒体基因组的基因组成较为保守,在58个线粒体基因组中,仅有3个物种存在基因数量的差异。在19个鳗鲡目鱼类线粒体基因组中存在主编码基因的重排。主编码基因的变异位点分析结果支持nad5、nad4和nad2基因作为cox1和lrRNA基因辅助的分子标记。鳗鲡科Anguillidae的20个种(亚种)聚在一起,强烈支持鳗鲡科为单系群(BPP=100)。鳗鲡科下属的3个类群(大洋洲类群、大西洋类群和印度洋-太平洋类群)也同时得到有力的验证(BPP均为100)。线鳗科Nemichthyidae和锯齿鳗科Serrivomeridae亲缘关系最近,二者聚类后,与鳗鲡科Anguillidae构成姊妹群(BPP=100)。在囊喉鱼亚目Saccopharyngoidei中,宽咽鱼科Eurypharyngidae与囊鳃鳗科Saccopharyngidae聚类(BPP=100),同时,单颌鳗科Monognathidae与月尾鳗科Cyematidae聚类(BPP=100),4个科聚在一支,支持囊喉鱼亚目为单系群(BPP=100)。在囊喉鱼亚目线粒体基因组中,3个物种(吞鳗Eurypharynx pelecanoides、拉文囊鳃鳗Saccopharynx lavenbergi和杰氏单颌鳗Monognathus jesperseni)存在主编码基因的重排。16种鳗鲡(细美体鳗Ariosoma shiroanago、短吻颈鳗Derichthys serpentinus、凯氏短尾康吉鳗Coloconger cadenati、鸭颈鳗Nessorhamphus ingolfianus、龟草鳗Thalassenchelys sp.、粗犁齿海鳗Cynoponticus ferox、百吉海鳗Muraenesox bagio、巨斑花蛇鳗Myrichthys maculosus、大吻沙蛇鳗Ophisurus macrorhynchos、几内亚副康吉鳗Paraconger notialis、哈氏异康吉鳗Heteroconger hassi、小头鸭嘴鳗Nettastoma parviceps、弱头鳗Leptocephalus sp.、斑点长犁齿鳗Hoplunnis punctata、尖吻小鸭嘴鳗Facciolella oxyrhyncha和星康吉鳗Conger myriaster),与鳗鲡目线粒体主编码基因的原始排列相比,共享nad6基因的易位。同时,基于线粒体基因组13个蛋白质编码基因构建的系统演化树,强烈支持这16个物种聚为一支(BPP=100)。然而,由此而带来的海鳗科Muraenesocidae、拟鯙科Chlopsidae和糯鳗科Congridae是否为单系群的问题,值得今后深入探究。     

九龙江口鳗苗溯河生态与资源研究

九龙江口Ｌｉ苗１９９４￣１９９６年溯河期为１９９４年１１月２０至１９９５年４月底。１９９５年１２月２０日至１９９６年５月底。鳗苗溯河期水温范围１１．５￣２２．５℃,盐度１．９８￣２５．００,透明度０．２￣１．１ｍ,九龙江口打石坑－嵩屿断面溯河鳗苗资源量１９９５年１９３ｋｇ,１９９６年２０８ｋｇ;海澄断面１９９５年３５ｋｇ,１９９６年５５ｋｇ。     

欧鳗(Anguilla anguilla)饲料

本文初步概述饲养欧洲鳗鲡（Anguillaanguilla）的食性特征，对蛋白质的表现消化率和日粮蛋白质的需求量、驯饵、饲料形态、给饲率以及实用的欧鳗配合饲料。建议采用膨化颗粒饲料，制订欧级饲料实验方法和条件的规范。     

Blood oxygenation in shortfin eels during swimming and hypoxia: Influence of the root effect

Blood samples were withdrawn from dorsal aortic cannulae in shortfin eels before, during, and after acute exposure to hypoxia and to forced swimming. Both hypoxia (PIO₂ < 40 mm Hg) and forced swimming at 24 cm s^‐1 abolished apnoeic periods and increased ventilation. The fall in PaCO₂with hyperventilation, raised pH and thus increased the oxygen carrying capacity of arterial blood. The Root effect influences shortfin eel blood up to pH 8.0, at 17°C and has an effect on arterial oxygen content. It cannot be responsible for all of the increased oxygen content during swimming and in recovery from swimming and hypoxia; ventilatory and/or circulatory changes must account for the rest. Hypoxia, but not swimming, produced a rise in dorsal aortic blood pressure. The bradycardia of hypoxia was deeper than that which accompanies apnoeic periods in air‐saturated water.     

台湾屏东县东港溪拦溪对其周边地下水水质及水产养殖的影响

为了解设于屏东县新园乡界河以东港溪上的拦河堰对当地地下水的影响进行一系列研究.首先对台湾西南沿海的屏东县新园乡1989年10月东港溪拦溪建堰前后地下水水质及等水位线数据进行了分析,同时逐月收集东港溪拦溪堰周边的佳冬乡养殖池所用的井水、池水、浮游生物、日本鳗鲡(Anguilla japonica)及虾等数据,进行分析与比较.此外,用以上2个乡的地下水进行了鳗鲡幼苗水族箱养殖试验,并检验分析试验后的鳗鱼.各项分析结果表明,拦溪堰的兴建使新园乡的地下水水位降低,附近污染河川的水渗入了地下,对养殖生物的健康产生不同程度的影响.因此建议,在旱季应减少对东港溪拦溪堰水的抽取量;台湾西部尤其是新园乡的水产养殖活动应予以减少.     

"合信"牌欧鳗配合饲料精养缓长型欧鳗的试验

本文报告了"合信"牌欧鳗配合饲料精养缓长型欧鳗的试验.结果表明，试验组的平均饲料效率为48.11％，比对照组提高了10.71％；欧鳗的存活率达99.9％，且有较好的肉质，符合出口烤鳗的要求.