胶州湾网采浮游植物群落结构的长期变化

依据20世纪80年代以来胶州湾浮游植物的调查资料,系统分析网采浮游植物群落的长期变化特征。结果表明,自1981年以来,胶州湾浮游植物总量呈现增加的趋势,但空间分布格局没有发生明显改变。胶州湾的浮游植物优势种组成发生改变,洛氏角毛藻、密联角毛藻、波状石鼓藻、叉角藻与梭角藻等成为近5年来新的优势种。胶州湾浮游植物优势类群数...     

2009年夏季胶州湾同步调查浮游植物群落结构特征

根据2009年8月17日14:00胶州湾21个大面站的同步调查资料,研究了夏季胶州湾浮游植物群落结构特征。共检出浮游植物3门35属65种,平均细胞丰度为77.2cell/m L,硅藻在物种数量和细胞丰度上占绝对优势。主要优势种为中肋骨条藻Skeletonema costatum和大洋角管藻Cerataulina pelagica。夏季胶州湾浮游植物表层细胞丰度的平面分布趋势为北部高于南部,垂直分布趋势为从表层至底层细胞丰度明显降低。表层浮游植物的Shannon-Wiener指数和均匀度指数的分布显示胶州湾西北部和南部浮游植物群落结构更加稳定。与环境因子的相关分析表明浮游植物细胞丰度与盐度呈负相关,与硝酸盐、亚硝酸盐、磷酸盐呈正相关。     

胶州湾浮游植物多样性及其与环境因子的关系

2003年6月对胶州湾水域中的浮游植物进行了调查,根据所获得的资料分析了胶州湾水域中浮游植物分布规律及优势种的组成、浮游植物多样性分布及浮游植物与环境因子的关系,结果表明：胶州湾浮游植物细胞数量及多样性分布受西部陆源污染的影响很明显;浮游植物中在细胞数量上占优势的种类有中肋骨条藻、劳氏角毛藻、加氏星杆藻、锤状中鼓藻及扭鞘藻;由于各种原因浮游植物与环境因子的相关性不是很明显。     

胶州湾营养盐及富营养化特征

根据2003—2004年在胶州湾的调查资料,探讨了该湾营养盐及其富营养化状态。通过spss12.0统计分析营养盐和各理化因子之间的两两相关性,认为溶解无机氮、活性磷酸盐和活性硅酸盐之间有一定程度相似的生物地球化学过程,且外源性营养盐是其主要的来源。频数分析显示,胶州湾硅酸盐浓度控制了SiO3-Si/PO4-P和SiO3-Si/DIN的比例,此二者多数情况下小于其各自的化学计量平衡。冬季营养盐和叶绿素a的消长关系及浮游植物赤潮优势种数量变化分析说明,硅酸盐的含量对调查海域赤潮的发生发展和消退起着重要的控制作用。这些表明,SiO3-Si是胶州湾浮游植物生长的潜在限制因子,特别是冬季。利用Matlab建立了人工神经网络富营养化评价模型,结果认为带有SiO3-Si因子的评价结果更好地反映了胶州湾的富营养化状况。     

胶州湾浮游植物研究Ⅱ环境因子对浮游植物群落结构变化的影响

该文依据 1995年 3月～ 1996年 1月间浮游植物的调查资料 ,并结合同期水温和营养盐的资料变化 ,分析不同环境因素对浮游植物群落结构的影响。结果表明 :水温的变化对浮游植物的种类组成和数量变化都有一定影响 ,秋季和冬季优势种交替明显 ;营养盐对浮游植物群落结构的变化影响明显。根据胶州湾营养盐结构的改变状况 ,并结合历史资料对比发现 ,胶州湾网采浮游植物的物种数和细胞数量呈下降趋势 ,优势种类趋单一化 ;但初级生产力并未下降 ,可能与微型浮游植物所占份额增大相关。     

胶州湾海域浮游植物和赤潮物种的生物多样性研究进展

胶州湾是我国的一个典型近海海湾,也是近海生态系统的缩影。历史上胶州湾海域赤潮频发,因此也是研究赤潮暴发机制(包括赤潮物种组成、时空动态变化及对环境因子响应)的理想实验场所。迄今,几代科学家对胶州湾浮游植物和赤潮物种开展了长期而系统的调查研究,获得了大量的研究成果。为了系统跟踪胶州湾海域浮游植物群落组成,尤其是赤潮物种的组成变化,本文对近84年间(1936—2019年)胶州湾海域涉及浮游植物调研的54个调查数据结果进行了系统的统计和比较分析。文献共报道了549种浮游植物,包括硅藻326种、甲藻164种、绿藻21种、金藻9种、隐藻9种、淡色藻6种、定鞭藻5种、蓝藻3种、黄藻3种、下睫虫2种、裸藻1种和针胞藻1种。其中只有不到一半(40.29%)的浮游植物的全长18S rDNA序列得到解析,表明分子标记数据库需要继续完善,促进宏条形码分析的有效应用。胶州湾海域的优势浮游植物随时间的推移发生了较大变化,但是报道的甲藻与硅藻物种数的比例没有显著的变化。统计表明,研究者在胶州湾共鉴定到153种赤潮物种,包括硅藻79种、甲藻67种、定鞭藻2种、金藻2种、淡色藻1种、黄藻1种和针胞藻1种。胶州湾海域的典型优势浮游植物均为赤潮物种,包括中肋骨条藻、短角弯角藻、旋链角毛藻、加氏星杆藻、日本星杆藻、星脐圆筛藻和尖刺拟菱形藻,其中短角弯角藻和中肋骨条藻是胶州湾海域的"常驻"赤潮物种。本文通过系统综述胶州湾浮游植物和赤潮物种的生物多样性,为利用宏条形码和宏基因组等方法跟踪研究胶州湾和其他海域的浮游植物和赤潮物种的演化奠定了基础。     

2001年夏季胶州湾浮游植物群落结构的特征

本文根据 2 0 0 1年 8月 2 0～ 2 2日期间对胶州湾海域进行的大面积调查资料 ,对浮游植物的群落结构进行了初步研究。结果表明 ,浮游植物群落主要由沿岸暖水性种类组成 ,以硅藻为主 ,还有少量的甲藻、金藻和绿藻。湾中央出现的种类最多 ,湾口其次 ,湾边缘最少。细胞数量的高值区出现在B断面和 A5站周围的水域 ,低值区出现 D断面和 E断面。此外 ,对群落多样性的研究表明 ,Shannon指数在湾北部的多数站位高于湾南部水域 ,在 D2和 B1站出现 2个低值区 ;Margalef指数在湾内东部和 D4站周围水域明显高于湾内西部和湾口水域 ;均匀度则在 B1和 D2站周围水域偏高。浮游植物群落同营养盐的相关分析表明 ,调查期氨盐和磷酸盐是影响浮游植物生长的主要营养盐 ,中肋骨条藻、浮动弯角藻和旋链角毛藻等几种优势种类决定了这一关系     

胶州湾海域浮游植物和赤潮物种的生物多样性研究

胶州湾是我国一个典型的近海海湾,也是近海生态系统的缩影。历史上胶州湾海域赤潮频发,因此也是研究赤潮暴发机制(包括赤潮物种组成、时空动态变化及对环境因子响应)的理想实验场所。迄今,几代科学家对胶州湾浮游植物和赤潮物种开展了长期而系统的调查研究,获得了大量的研究成果。为了系统跟踪胶州湾海域浮游植物群落组成,尤其是赤潮物种的组成变化,解读基于分子标记的宏条形码分析结果,本文对近84年间(1936年-2019年)胶州湾海域涉及浮游植物调研的54个调查数据进行了系统的统计和比较分析。文献共报道了549种浮游植物,包括硅藻326种、甲藻164种、绿藻21种、金藻9种、隐藻9种、淡色藻6种、定鞭藻5种、蓝藻3种、黄藻3种、下睫虫2种、裸藻1种和针胞藻1种。其中只有不到一半(40.29%)的浮游植物的全长18S rDNA序列得到解析,表明分子标记数据库需要继续完善,促进宏条形码分析的有效应用。胶州湾海域的优势浮游植物随时间发生了较大变化,但是甲藻与硅藻数目的比例没有显著的变化。统计表明,胶州湾共鉴定到153种赤潮物种,包括硅藻79种、甲藻67种、定鞭藻2种、金藻2种、淡色藻1种、黄藻1种和针胞藻1种。胶州湾海域典型的优势赤潮物种包括中肋骨条藻、短角弯角藻、旋链角毛藻、加氏星杆藻、日本星杆藻、星脐圆筛藻和尖刺拟菱形藻。本文通过系统综述胶州湾浮游植物和赤潮物种的生物多样性,为利用宏条形码和宏基因组等方法跟踪研究胶州湾和其他海域的浮游植物和赤潮物种的演化奠定了基础。     

胶州湾冬季浮游植物光合作用特征原位研究

目前关于中国近海浮游植物光合作用特征的研究较少,尤其是海洋浮游藻类冬季光合生理生态方面的研究少见报道。本实验利用浮游植物荧光仪Phyto-PAM测量了胶州湾浮游植物最大光化学量子产量、快速光曲线、光化学淬灭以及非光化学淬灭,通过显微镜镜检获得浮游植物群落结构组成与丰度结果,结合相应的环境因子,对胶州湾冬季浮游植物进行了原位光合作用特征研究。结果表明:调查期间胶州湾浮游植物具有较高的光合活性与潜力,最大光化学量子产量保持在0.69左右,光能利用效率大部分处于0.2—0.3之间,快速光曲线十分典型,当光强超过1000μmol/(s·m2)后依然能保持较好的光合活性,光化学淬灭值较高;当光强超过1500μmol/(s·m2)后,相对电子传递速率开始下降,实际光量子收益达到最低,非光化学淬灭升高,此时海洋浮游植物将光合机构吸收的过量光能以热能的形式耗散掉,有效地保护了胶州湾浮游植物的光合器官不因高光强的照射而受到损伤。冬季大部分时期胶州湾浮游植物丰度极低,但是湾内浮游植物具有较强的光合活性,加上相对稳定的水文、生物等环境以及较丰富的营养盐和相对有利的营养盐结构,是推动冬季浮游植物高峰期出现的关键生理生态因素。     

胶州湾营养盐结构的长期变化及其对生态环境的影响

根据 1 962年— 1 998年 81个航次的调查和历史资料 ,讨论了胶州湾营养盐及其结构的长期变化和对生态环境的影响。近 40年来 ,胶州湾无机N和P浓度分别增加了 3.9倍和1 .4倍 ,DIN/PO4 P摩尔比从 1 5 .9± 6.3增加到 37.8± 2 2 .9,SiO3 Si浓度保持在一个很低的水平。高的DIN/PO4 P比例和很低的SiO3 Si/PO4 P比例 ( 7.6± 8.9)及SiO3 Si/DIN比例 ( 0 .1 9±0 .1 5 )表明 ,胶州湾营养盐结构已经从比较平衡到不平衡。根据化学计量营养盐限制的标准 ,DIN和PO4 P作为胶州湾浮游植物限制因子的可能性已经减少或消失 ,SiO3 Si限制已经增加。营养盐结构的变化已经导致大型硅藻的减少和浮游植物优势种组成的变化 ,大型硅藻可能趋向于小型化。胶州湾生态系统的这些变化是人类活动影响的直接结果。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Assessing the Validity of Seismo-Acoustic Predictor Equations for Obtaining Seabed and Sub-Surface Sediment Physical Properties

The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.     

A Review of Some Environmental Issues Affecting Marine Mining

This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested.     

Increased Particle Fluxes at the INDEX Site Attributable to Simulated Benthic Disturbance

Particle fluxes were measured 7 m above the sea bottom during the predisturbance, disturbance, and postdisturbance periods by using time series sediment traps attached to seven deep-sea moorings deployed in the INDEX experiment site in the Central Indian Basin. The predisturbance particle fluxes varied between 22.3 to 55.1 mg m -2 day -1 . Increased and variable particle fluxes were recorded by the sediment traps during the disturbance period. The increase observed was 0.5 to 4 times more than the background predisturbance fluxes. The increases in particle fluxes (~4 times) recorded by the sediment trap located in the southwestern direction (DMS-1) were the greatest, which could be the result of preferential movement of resuspended particles generated during the deep-sea benthic disturbance along the general current direction prevailing in this area during the experimental period. Also, the traps located closer to the disturbance area recorded greater fluxes than did the traps far away, across the Deep Sea Sediment Resuspension System path. This variability in recorded particle fluxes by the traps around the disturbance area clearly indicates that physical characteristics such as grain size and density of the resuspended particles produced during the disturbance had an important effect on particle movement. The postdisturbance measurements during ~5 days showed a reduction in particle fluxes of ~50%, indicating rapid particle settlement.     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Determination of In Situ Sediment Shear Strength from Advanced Piston Corer Pullout Forces

The advanced piston corer (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments.     

Interannual Sea Level Variations and Annual Tides in the Northwestern Pacific

Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.     

Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects

The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.