福建省主要河口海湾沉积物中甲藻休眠孢囊分类学初步研究

通过对福建省厦门湾、闽江口海域、三沙湾三个主要河口海湾144个站位表层沉积物样品中甲藻休眠孢囊的系统分析,根据甲藻休眠孢囊的形状、外壁结构、颜色、表面修饰物等形态特征共鉴定出9属25种及不定种,并对各孢囊属种的特征进行了分类描述,其中有毒甲藻休眠孢囊2种,即Alexandrium minutun,A. tamarense, 有害种类4 种, 即Alexandrium affine,Lingulodiniumpolyedrum,Scrippsiella trochoide.通过对比表明Alexandrium affine,A. minutum,Diplopelta cf. parva,Polykrikos cf. schwartzii,Protoceratium reticulatum,Protoperidinium minutum,P.cf. minutum,P. cf. americanum以及Alexandrium sp.,Protoperidinium sp.1,P. sp.2等11种甲藻休眠孢囊为本次在福建省海域新发现的种,这些新发现的甲藻种类可能是近年来通过国际间往来的航运船只在我国沿岸海域排放压舱水或贝类引种带来的.     

象山港,三门湾潮间带生态学研究：Ⅰ.种类组成与分布

象山港和三门湾地处浙江沿岸带中部，这两个半封闭型港湾的软相潮间带底栖动物种类组成均以广温广布性种占多数，其次为亚热带性种。种类的分布与盐度、潮流流速、悬沙含量以及港湾屏蔽程度等环境因子有关。象山港种类多于三门湾，同一港湾种类呈现湾底多于湾中多于湾口的特点。     

杭州湾期间带生态学研究──Ⅰ．种类组成与分布

对杭州湾软相潮间带周年调查，共获底栖动物９５种，以甲壳动物（３４种）、鱼类（２８种）、软体动物（２１种）占绝对优势。生态类群以我国沿岸广温广盐性种和半成水特有种为主，生活方式多为活动性和埋栖类型。这与杭州湾水域盐度低且变化幅度大及动力作用强，底质不稳定等因素密切相关。从湾底到湾口，底栖动物种类增多，组成类群逐渐从低盐的河口种向广盐的海洋种演替，表现出明显的湾性分布。种类的垂直分布是中潮区大于低潮区大于高潮区，各类群生物在潮间带呈现随机镶嵌分布，无明显的分带现象。种数的季节分布呈现更、秋两季较多、冬季最少的特点。     

厦门岛东海岸沙滩底栖甲藻的种类组成及优势种的季节变化

2001年8月至2002年7月,对厦门岛东海岸黄厝沙滩底栖甲藻的种类组成和优势种的时空分布进行了为每月一次的调查,共鉴定甲藻种类46种,其中底栖种39种,常见种14种,优势种4种.Amphidinium britannicum和Adenoides sp.为冬春季优势种,Herdmania litoraris和Peri-dinium quinquecorne为夏秋优势种,优势种的季节更替比较明显.夏秋季节的种数多,冬春季节的种数少,细胞数量高峰出现在春夏季,秋冬两季数量很少,由夏季的200多个每克干沙减少到冬末的0~4个/g干沙;多样性指数和均匀度呈双峰型分布,峰值出现在10和2月,谷值出现在12月和7月.空间分布上,底栖甲藻的总细胞密度和优势种的细胞密度呈现由高潮带向中、低潮带增加的趋势.分析了影响厦门东海岸沙滩底栖甲藻时空分布的主要因素,并探讨了沙滩底栖甲藻与近岸赤潮及着色砂现象的关系.     

乐清湾大型底栖动物种类和数量组成特征及变化

为了解和掌握乐清湾大型底栖动物种类和数量组成特征及变化情况,本研究于2015年1月、3月、5月、8月和10月进行5个航次的大型底栖动物调查。结果表明,乐清湾共有大型底栖动物116种,环节动物为主要优势类群;种类组成、栖息密度和生物量在不同航次之间均存在较大幅度的波动;相同航次之间的种类组成、栖息密度和生物量在空间分布也存在明显斑块化。通过对比分析历史研究数据发现乐清湾的大型底栖动物种类和数量组成变化明显,其中小个体环节动物的种类数和栖息密度明显升高,而大个体软体动物和棘皮动物的种类数和栖息密度则下降明显。这说明乐清湾底栖生境处于不稳定状态,需要在长期稳定的生境中生长的大个体动物不能得到充分的生长,取而代之的是出生率高,生长周期短的小个体r-对策者的快速繁殖。     

浙江海岸带表层沉积物中有孔虫的分布及其影响因子

本文通过对浙江沿岸潮间带和水深小于20m浅海区的102个表层沉积样品的有孔虫分析,发现研究区内有孔虫的数量和组合存在明显差异。潮间带底栖和浮游有孔虫的数量明显低于浅水区,底栖有孔虫数量分布趋势是由北向南逐渐增加,而浮游有孔虫恰好与底栖有孔虫相反。除杭州湾外,潮间带的浮游有孔虫相当稀少。根据有孔虫动物群的数量及分布特点,研究区的底栖有孔虫可分为3个组合和5个亚组合。文中还叙述了玻璃状壳,似瓷状壳和胶结壳等各类壳的分布特点。最后讨论了有孔虫分布与水深、温度、盐度、搬运作用和“稀释”作用等的相互关系。     

象山港,三门湾潮间带生态学研究：Ⅲ.群落结构

本文应用数理统计的方法研究了象山港、三门湾软底相潮间带中７个断面的底栖动物群落结构。根据断面的位置及其群落的变化特点，可以比较明显地分为开敞性程度高的湾口群落和屏蔽性程度高的湾底群落。湾口群落不稳定、群落结构季节变化较大，湾底群落则相反，并且形成代表群落的特征种。从湾底到湾口，群落的Ｓｈａｎｎｏｎ－Ｗｉｅｎｅｒ多样性指数Ｈ＇（Ｓ）和Ｐｉｅｌｏｕ均匀度Ｊ均增加，而Ｍａｒｇａｌｅｆ种类丰度ｄ和ＭｃＮａｕｈｏｎ优势度Ｄ均减少。对环境诸因子的聚类分析结果基本上与相似性群落数量的聚类分析结果相同，表明群落的形成与环境因子的影响有一定的关系。     

长江口海域春季浮游植物的年际变化

于2014年5月在长江口海域采集海水样品,分析了浮游植物种类组成、细胞丰度、优势种等群落结构特征。共鉴定浮游植物105种,其中硅藻30属56种,甲藻19属41种,以及隶属于金藻、蓝藻等的8个种。浮游硅藻以近岸半咸水种为主,优势种类是中肋骨条藻(Skeletonema costatum);浮游甲藻以沿岸广布种为主,典型代表是东海原甲藻(Prorocentrum donghaiense)。为了更好地了解长江口海域浮游植物的年际变化趋势,本文收集整理了该海域1986—2014年间的春季数据,分析了浮游硅藻、浮游甲藻主要群落特征的年际变化,以及浮游硅藻优势种类细胞粒径的变化趋势。结果表明:(1)浮游硅藻、浮游甲藻的种类数均呈现不同程度的上升趋势,浮游甲藻的上升幅度更为明显;(2)浮游甲藻种类数在浮游植物种类数中的占有比例呈上升趋势,而浮游硅藻所占比例呈下降趋势;(3)浮游硅藻优势种类的细胞粒径呈现小型化趋势。     

南极长城湾全新世有孔虫研究

南极长城湾９３Ｉ（６２°１７．７４′Ｓ，５８°４９．９１′Ｗ）柱样含有较丰富的有孔虫动物群，计鉴定统计５１９００个体，归属于８６种，有孔虫总丰度以柱样中段较低，浮游类含量较低（〈５％）钙质类底栖有孔虫成占优势，全柱底栖有孔虫分异度变化范围不大，底栖有孔虫五个优势种的优势度占决量的８３．３％，优势种及其组合变化显著一定的古海洋学意义，探讨了长城湾海区晚第四纪环境变迁，为南大洋晚第四纪环境与全球变化关     

泉州湾蟳埔潮间带大型底栖动物功能群研究

为了比较不同生境的大型底栖动物功能群,根据在泉州湾蟳埔潮间带3种生境类型获得的大型底栖动物数据进行了分析。2011年4月-至2012年1月在泉州湾蟳埔潮间带获得大型底栖动物101种,其中浮游生物食者(Pl)、植食者(Ph)、肉食者(C)、杂食者(O)和碎屑食者(D)物种数分别为21种、18种、21种、26种和15种。光滩(沙滩)、互花米草和牡蛎石三种生境大型底栖动物物种数、平均栖息密度、平均生物量、多样性指数的优势功能群多样化,表明泉州湾蟳埔潮间带大型底栖动物功能群的复杂化和多样化,这种特征是潮汐、生境、底质粒径等环境因子共同作用的结果。潮汐导致潮间带的空间异质性(沉积物粒径的差异),空间异质性导致大型底栖动物功能群组成的差异。互花米草、牡蛎石构成了多种小生境,有利于众多大型底栖动物的栖息。还讨论了大型底栖动物物种鉴定水平和功能群划分标准不同对功能群研究结果的影响。     

Dinoflagellates in a mesotrophic,tropical environment influenced by monsoon

The changes in dinoflagellate community structure in both – the water column and sediment in a mesotrophic, tropical port environment were investigated in this study. Since the South West Monsoon (SWM) is the main source of climatic variation, observations were made during two consecutive post-monsoon periods (2001 and 2002) and the intervening pre-monsoon period (2002). The pre-monsoon period supported a more diverse dinoflagellate community in the water column compared to both post-monsoon periods. Heterotrophic dinoflagellates were abundant in the water column as well as sediment. A seasonal cycling between vegetative and resting cysts of autotrophic and heterotrophic dinoflagellates governed by the environmental characteristics of the study area was observed. Temperature, salinity and suspended particulate matter were the main factors affecting dinoflagellate community structure in both the water column and sediment. The dominant dinoflagellates in the water column differed during both post-monsoon periods that followed two dissimilar monsoon events. Prorocentroids and gonyaulacoids dominated the water column subsequent to the 2001 SWM, whereas dinophysoids and unidentified tiny dinoflagellates dominated during the next post-monsoon period. The 2001 SWM started in May, peaked during June–July and reduced gradually to end in October. The 2002 SWM was erratic; it started late (in June) and ended earlier (in September). These observations highlight the potential of the SWM to influence the community structure of dinoflagellates in tropical waters and points to the importance of long-term studies to discern robust variations in dinoflagellate communities in response to fluctuating monsoon regimes.     

甲藻赤潮与水体中营养盐的关系初探

作者在国家海洋局第三海洋研究所的陆基水池中，应用围隔实验技术，排除了水动力、盐度等环境因子的变动性的干扰，人工诱发甲藻赤潮．在这个围隔实验的两个围隔体中，分别发生了一种裸甲藻（Gymnodinium sp.）和海洋原甲藻（Prorocentum micans）的赤潮．在赤潮发生和发展过程中所监测到的营养盐数据表明有些甲藻增殖不一定需要水体中高营养盐；营养盐供给的多寡会导致甲藻赤潮优势种的差异和赤潮规模．     

台湾海峡浮游甲藻的分布特征和变化趋势

根据2006-2007年“国家908”专项台湾海峡4个航次调查,分析该海域浮游甲藻的种类组成、群落结构和时空分布特征。共鉴定甲藻18属131种,其中高温高盐种为主体,占总种数的72．52％,其次为广温广盐种,占总种数的25．19％,近岸种仪占2．29％。夏季甲藻的种类最丰富,而春季甲藻的丰度最高。平均丰度为404．96×10^2cells／m3,其平面分布呈现从近岸向外海、从北向南递增的趋势。与1984-1985年的调查结果相比较,甲藻丰度的平面分布格局和季节变化趋势没有明显变化,但丰度增加3．01倍。从季节变化看,冷季甲藻丰度增加较为显著;从平面分布看,台湾海峡北部增加较多。此外,该海域甲藻群落结构也发生变化,种类多样性指数和均匀度上升,其丰度占浮游植物总丰度的比例由0．55％上升到1．02％。     

大亚湾大鹏澳海域表层沉积物中浮游植物休眠体集群的季节变化

于2007年4月至2008年3月对大亚湾大鹏澳海域表层沉积物中浮游植物休眠体进行了每月1次的周年调查.本调查分析鉴定出浮游植物休眠体79种,包括甲藻孢囊50种,硅藻休眠体26种.浮游植物休眠体丰度没有明显的季节变化规律,变化范围为217～6 764 cells/cm3,平均为1 376 cells/cm3;鱼类养殖区休眠体丰度较高,平均丰度为1 999 cells/cm3.浮游植物休眠体在数量上以硅藻为主,甲藻次之,而在鱼类养殖区也出现了较高的蓝藻休眠体.硅藻休眠体的平均丰度为783 cells/cm3,百分比含量为56.9%,从而反映了该海域硅藻型浮游植物群落结构.甲藻孢囊丰度的变化范围在79～1 237 cells/cm3,位于湾口对照区丰度较高,年平均为733 cells/cm3,而鱼类养殖区和贝类养殖区平均值分别为502、388 cells/cm3.异养型甲藻孢囊的种类和数量均比较丰富,其百分比含量为26.7%～82.4%.研究结果显示,在富营养化程度较高的养殖区海域,硅藻休眠体和异养型甲藻孢囊百分率均较高,而鱼类养殖区还出现了较高蓝藻孢子丰度.本次调查中还出现了易碎藻和艉杆藻等在本海域浮游植物调查中未记录种类的孢囊,有毒有害赤潮生物的休眠体如亚历山大藻、链状裸甲藻、旋沟藻、巴哈马梨甲藻孢囊等也在此次调查中频繁出现,说明大亚湾海域存在较大的有毒有害赤潮发生的风险.     

Blooms of Prorocentrum donghaiense reduced the species diversity of dinoflagellate community

Most of reported harmful algal blooms(HABs)of microalgae(75%)have been caused by dinoflagellates.Studies on the negative effects of HABs have generally focused on animals,valuable organisms in particular,and environmental factors such as dissolved oxygen and nutrients,but relatively fewer on community level,particularly that using metagenomic approach.In this study,we reported an investigation on the effects of a HAB caused by the dinoflagellate Prorocentrum donghaiense on the species diversity and community structure of the dinoflagellate sub-community via a pyrosequencing approach for the samples taken before,during,and after the bloom season of P.donghaiense in the East China Sea.We sequenced partial 28S rRNA gene of dinoflagellates for the field samples and evaluated the species richness and diversity indices of the dinoflagellate community,as a sub-community of the total phytoplankton.We obtained 800185 valid sequences(categorized into 560 operational taxonomic units,OTUs)of dinoflagellates from 50 samples and found that the biodiversity of dinoflagellate community was significantly reduced during the blooming period in comparison to that in pre-and after-blooming periods,as reflected in the four diversity indices:the species richness expressed as the number of OTUs,Chao1 index,Shannon index(evenness),and Gini-Simpson index.These four indices were all found to be negatively correlated to the cell density of the bloom species P.donghaiense.Correlation analyses also revealed that the P.donghaiense cell abundance was correlated negatively with NO3--N,and NO2--N,but positively with total nitrogen(TN)and total phosphorus(TP).Principal coordinates analysis(PCoA)showed that the community structure of dinoflagellates was markedly different among the different sampling periods,while the redundancy analysis(RDA)revealed P.donghaiense abundance,salinity,NO3--N,and SiO32-were the most four significant factors shaping the dinoflagellate community structure.Our results together demonstrated that HABs caused by the dinoflagellate P.donghaiense could strongly impact the aquatic ecosystem on the sub-community level which the blooming species belongs to.     

舟山港4艘商船压舱箱沉积物中甲藻孢囊种群结构的分析

为了解商船压舱箱沉积物在海洋水生生物入侵过程中扮演的潜在媒介作用,本文利用光学显微镜观察法,研究了停泊于我国舟山港4艘商船压舱箱沉积物中甲藻孢囊的种类组成、孢囊密度、香农-威纳指数(H′)和PSP原因种,并对甲藻孢囊种群结构进行了相关性分析。结果表明,鉴定出的甲藻孢囊隶属于5大类,共24种,4份样品中甲藻孢囊的种群结构各有差异,但均检测出能产生麻痹性贝毒(Paralytic Shellfish Poisons,PSP)的甲藻孢囊,如亚历山大藻Alexandriumspp.和链状裸甲藻Gymnodium catenatum。在4艘商船的压舱箱沉积物中均检测出有硅藻分布,其中在富成轮压舱箱沉积物中还检测出3种未知种孢囊。通过相关性分析可知,甲藻孢囊H′值与PSP甲藻孢囊密度占总甲藻孢囊密度的比率呈负相关。甲藻可以通过商船压舱箱沉积物为传播媒介,以孢囊形式来扩大其生物地理分布,提高甲藻物种多样性可有助于降低PSP甲藻的破坏风险。因此,有效处理船只压舱箱沉积物、积极保护当地海洋生物物种多样性,对于降低外来甲藻物种(特别是一些有毒有害种)的赤潮风险和保持当地海洋生态系统的平衡,具有十分重要的意义。     

Contribution of benthic and epiphytic diatoms to clam and oyster production in the Akkeshi-ko estuary

The species composition and dynamics of phytoplankton in the water column and its contribution to clam, Ruditapes philippinarum, and oyster, Crassostrea gigas, production were studied in the Akkeshi-ko estuary, eastern part of Hokkaido, Japan. A total 128 taxa of diatoms were identified, with 103 and 102 species occurring on the surface sediments and in the water column, respectively. Amphora sp., Bacillaria paradoxa var paxilifer, Cocconeis scutellum, Navicula sp., Nitzschia sigma, Paralia sulcata, Rhoicosphenia curvata, Synedra ulna and Thalassiosira sp. were most common and dominant in all stations in both water column and surface sediment. Benthic diatoms were most dominant in both water column and surface sediment. The species composition of epiphytes was, in part, similar to diatom assemblage of the water column and also to that of the surface sediment. The benthic and pelagic ratio in the diatom assemblage of the water column was very high. Benthic diatoms were able to resuspend into water column by turbulence stimulated by wind. Chlorophyll a concentration in the water column increased as wind speed increased. Seventy and 67% of the gut contents oysters consisted of benthic diatoms in 2003 and 2004 and also 78 and 87% of clams in 2003 and 2004, respectively. The availability of benthic and epiphytic diatom assemblages contributes significantly to food resources for clam and oyster mariculture due to resuspension by disturbance in the Akkeshi-ko estuary.     

快速光响应曲线的优化及在底栖甲藻光适应特性研究中的应用

利用快速光响应曲线(RLCs)技术研究了不同光背景下的底栖甲藻Cooliatropicalis的光合作用特性,比较了RLCs的不同序列(正序、反序和非序)和不同光照时间(10, 30和60 s)对光合参数的影响。结果表明:正序RLCs由于序列内非光化学淬灭(NPQ)的快速累积,造成曲线的不饱以及光合参数最大相对电子传递速率(rETRmax)和半饱和光强(Ek)的高估。反序和非序RLCs均出现光饱和或光抑制,步长30s和60s的光合参数在不同光背景下的趋势基本一致。在底栖甲藻的光合生理研究中,可考虑采用步长30 s的反序RLCs进行测定。     

On an extremely dense bloom of the dinoflagellate Alexandrium tamarense in lagoons of the PO river delta: Impact on the environment

An extremely dense bloom of the potentially toxic dinoflagellate Alexandrium tamarense was observed in the lagoons of Cà Pisani (Veneto, Italy) in summer 1993. The lagoons were experiencing a significant eutrophication impact, receiving waste waters from intensive fish culture plants. During their bloom dinoflagellates in the lagoons reached densities of 2 to 4 × 10⁶ cells·dm⁻³ and a biomass of over 100 g·m⁻³. The bloom produced drastic ecological changes in the lagoons. It caused nocturnal anoxia, mortality of macrophytes and the build-up of labile organic matter in the water column. Grazing by the tintinnid Favella sp. contributed to the termination of the bloom of the flagellates. The results show that coastal aqua culture probably stimulates dinoflagellate blooms in shallow brackish lagoons.