三门湾网采浮游植物季节变化及影响因素

于2015—2016年春(5月)、夏(8月)、秋(11月)和冬(1月)四个航次对三门湾18个站位进行拖网采集和理化因子测定,分析了三门湾浮游植物群落结构、优势种组成、时空分布和主要影响因素,并结合历史数据,分析了浮游植物群落结构的变化规律,探讨了其主要驱动因子。调查共检出浮游植物8门87属199种(含变种、变型和未定名种),其中硅藻51属149种,甲藻18属27种,绿藻、裸藻、隐藻、定鞭藻、金藻和蓝藻偶有检出。四季共检出优势种8属13种,其中琼氏圆筛藻(Coscinodiscus jonesianus)为全年优势种。浮游植物年均丰度为1632.04×10~4cell/m~3,其中春季最低(53.06×10~4cell/m~3),夏季最高(5548.91×10~4cell/m~3)。聚类和相似性分析结果表明,浮游植物群落季节变化和区域差异明显。典范对应分析表明,影响三门湾海域浮游植物群落的主要因子依次为温度、盐度、DIN和DSi。结合历史资料发现,三门湾浮游植物群落丰度总体呈增加趋势(排除20世纪80年代冬季赤潮影响),主要种类组成仍以硅、甲藻为主,赤潮藻及嗜氮性硅藻种类数及优势度均上升。此外,三门湾浮游植物由大型硅藻向链状硅藻演替。海域富营养化和水温上升可能是主要驱动因子。     

珠江口羽流锋浮游植物群落对大气沉降的生态响应

受季风强迫, 珠江淡水羽在南海北部广泛形成羽流锋面, 而浮游植物群落通常在羽流锋面具有较高的生物量。南海北部毗邻珠江三角洲, 陆源输入的大气沉降对该海区生态系统影响显著。利用2019年3月珠江口西部的现场调查数据, 结合羽流锋面浮游植物群落分布的空间差异与大气沉降影响下的浮游植物群落生态学, 分析两者对南海北部陆架区浮游植物粒径群落以及微微型浮游植物的耦合影响。研究表明, 羽流锋面滨侧以小型(Micro)浮游植物群落为主, 锋面海侧以微微型(Pico)浮游植物群落为主, 而锋面区浮游植物群落粒径分布较为均匀, 且生物量高。锋面区域环境因子差异导致浮游植物群落分布呈现较大的空间差异。在锋面区域, 浮游植物群落生长总体受氮限制, 而聚球藻的生长则受磷限制。锋面区域浮游植物群落内部种群对营养盐的需求和响应有所差异。大气干、湿沉降的添加均能够促进不同粒径浮游植物群落的生长: 在锋面滨侧和锋面海侧, 小型浮游植物群落在添加气溶胶颗粒或雨水后比微型(Nano)和微微型浮游植物群落表现出更大的竞争优势, 生长率最高; 而锋面区域浮游植物群落表现出更强的适应性, 小型、微型、微微型浮游植物群落的生长速率均增加且无显著差异。大气沉降颗粒的添加显著促进了锋面系统微微型真核浮游植物与锋面滨侧聚球藻的生长, 在锋面区域以及锋面海侧则抑制了聚球藻和原绿球藻的生长。     

海南东部沿岸河流和潟湖中溶存甲烷的分布及通量

甲烷(CH4)作为大气中重要的温室气体,直接或间接地影响全球气候变化。于2009年3月27日至4月15日对海南东部河流(文昌河、文教河、万泉河)、潟湖(八门湾、博鳌、小海和老爷海)等进行了调查,采集了表层和部分底层水样,对溶解CH4浓度进行了测定并初步估算了其水-气交换通量。结果表明,文昌河、文教河受人为活动影响较大,表层CH4饱和度较高,分别为(60664±25118)%、(38582±26339)%,污水输入、红树林输入和现场产生是其CH4的主要来源。万泉河表层CH4饱和度为(9472±5594)%,现场产生是其水体溶存CH4的主要源。八门湾、博鳌、小海和老爷海潟湖表层水体溶存CH4均处于过饱和状态,但其饱和度远小于入湖河流,分别为(2471±2937)%、(5692±3435)%、(546±251)%和(6878±4635)%,其中河流输入、地下水和红树林间隙水输入、现场产生是潟湖中CH4的主要来源,水体中CH4的氧化和水-气交换是潟湖中CH4的主要汇。初步估算出文昌河和万泉河CH4的水-气交换通量分别为5967.0±5142.1和496.2±335.9μmol·m 2·d 1,八门湾、博鳌、小海和老爷海潟湖的水-气交换通量分别为528.7±625.0、441.7±473.3、26.6±21.6和1287.8±1453.3μmol·m 2·d 1。海南东部河流、潟湖是近岸水体及大气CH4的净源。     

印度尼西亚北部苏拉维斯蓝碧海峡2012-2015年东南季风期间浮游植物的变化

2013年4月,2014年5月,2012年6月和2015年10月在印度尼西亚北部苏拉维斯蓝碧海峡（Lembeh Strait）进行了4个航次的海洋生态调查,研究了蓝碧海峡的浮游植物种类组成,以及浮游植物群落结构的季节变化及其驱动因子。四个航次的调查中记录了5类浮游植物和416个种（类）。调查区浮游植物密度平均为2348cells/L,硅藻和甲藻种类最多。蓝藻种类最少,但丰度很高。季风转换期的4月份和10月份浮游植物丰度总量较低,东南季风期间5月份和6月份的浮游植物丰度较高。除了束毛藻（Trichodesmium）之外,常见种是浮游硅藻。浮游植物的丰度和多样性的季节性变化明显。海链藻（Thalassionema）、拟菱形藻（Pseudo-nitzschia）和蓝藻类的束毛藻对群落的不相似性贡献最大。由于蓝碧海峡南部营养盐浓度较高,南部水域的硅藻和甲藻密度稍高于海峡北部。虽然,南北两个水体中蓝藻的分布趋于均匀,但蓝碧海峡南部也有较高密度。与雅加达湾以及中国的一些海湾相比,蓝碧海峡的浮游植物丰度相当低。分析表明,SE季风期间上升流营养盐是左右浮游植物丰度月变化的重要因素。蓝碧海峡浮游生态系原始,且接近天然本底,人类活动背景噪声低,能够清晰地响应大环境的变化,是研究浮游生态系统的理想区域。     

大亚湾夏季浮游植物群落结构及对淡澳河输入的响应特征*

浮游植物是水生生态系统的基础生产者, 其群落结构直接影响到生态系统的健康和安全。河流输入是人类活动影响大亚湾水体环境最重要的途径之一, 淡水输入改变了水体温度、盐度、浊度和营养盐等环境因子, 对浮游植物群落结构产生影响。文章调查研究了2015年河流输入最强的夏季丰水期大亚湾的水体环境因子和浮游植物群落结构, 分析了在较强河流输入影响下浮游植物群落结构的动态变化及其对环境因子的响应。结果发现, 夏季大亚湾淡澳河的输入使湾顶淡澳河口区域形成层化的低盐、高温、低透明度、高营养盐的水体, 湾中部表层水体则受一定强度河流羽流影响, 而湾口和湾中部底层水体主要受外海水影响。淡澳河淡水输入是夏季大亚湾外源性氮、磷营养盐的主要来源, 而硅酸盐除河流输入外, 外海水也输入较多的营养盐使得底层水体硅酸盐浓度较高。夏季大亚湾水体营养比例失衡较严重, 溶解无机磷是限制浮游植物生长的重要因子。硅藻是大亚湾夏季浮游植物的优势类群, 调查发现3种优势种[极小海链藻(Thalassiosira minima)、中肋骨条藻(Skeletonema costatum)和圆海链藻(Thalassiosira rotula)]均为硅藻。通过聚类分析, 可将大亚湾夏季浮游植物群落主要分为3种类型, 分别为: 浮游植物丰度较大的极小海链藻藻华暴发的群落, 位于淡澳河口, 受河流输入影响明显; 中肋骨条藻占据优势的群落, 分布在受一定强度的河流及其羽流影响的湾顶和湾中部区域; 浮游植物丰度较低的群落, 无明显优势种, 主要分布在湾口海水影响区域。淡澳河口的水体环境有利于小型链状硅藻极小海链藻的快速繁殖并暴发了藻华, 藻华发生时的海水环境条件为: 温度30~31°C, 盐度17‰~31‰, 水体透明度0.45~1.2m。硅藻对不同营养盐利用的差异以及随后的生物碎屑和颗粒沉降过程导致藻华发生区域Si∶N值略降低, N∶P值显著升高。河流输入影响下, 单一物种大量生长使得浮游植物群落种类组成丰度分布极不均匀, 从而导致淡澳河口浮游植物群落的种类多样性和均匀度指数降低, 种类多样性和均匀度指数均从淡澳河口向湾口逐渐增大。     

珠江口海域营养盐比及与浮游植物的关系

根据2004-2006年珠江口海域的调查资料,对营养盐含量和比值与浮游植物群落结构的关系进行分析.结果显示,调查海域维持丰富的氮、磷、硅,其中氮、硅以东宝河口最高,磷以深圳湾最高;丰富的氮、磷、硅和较高的N:P均可能是形成调查海域多年来浮游植物群落以硅藻类占优势的主要原因;但是在深圳湾、桂山岛、万山列岛及横琴岛附近海域(主要是4月),营养盐含量及其之间的比例发生变化(深圳湾主要是 N:P,后三个岛屿附近海域主要是Si:N),对浮游植物群落结构变化产生影响;并且近年来珠海高速发展的养殖业造成其海域赤潮的频发.     

三峡水库蓄水前后长江口及其邻近海域浮游植物群落结构的变化及原因分析

通过对长江口及其邻近海域2002-2006年13个航次的综合调查,研究了三峡水库蓄水前后长江口及其邻近海域浮游植物群落结构的变化,并应用相关性分析、典范对应分析(CCA)等方法对浮游植物群落结构变化的原因进行了探讨。结果表明:三峡水库蓄水后,长江口及其邻近海域的浮游植物群落结构、盐度及营养盐结构已发生显著变化。硅藻种类比例显著下降,由2002年秋季的77.09%下降至2006年秋季的71.43%;甲藻种类比例显著上升,由17.32%上升至22.45%。主要环境因子中,磷酸盐(DIP)、硅酸盐(DSi)、无机氮(DIN)浓度显著下降,盐度、N/P比值显著上升。DSi的降幅最大,从2002年秋季的83.14μmol/L下降至2006年秋季的42.83μmol/L;DIP从1.40μmol/L下降至0.89μmol/L;DIN从56.80μmol/L下降至49.18μmol/L。而N/P比值从39.86上升至81.27;盐度则从19.44上升至25.42。CCA及相关性分析表明,盐度、N/P比值的升高,DIP、DSi浓度的降低,均对浮游植物群落结构变化产生了显著的促进作用。三峡水库蓄水后,长江径流量、输沙量的减少以及径流季节分配的改变是造成长江口及其邻近海域浮游植物群落结构发生改变的根本原因。     

福建沙埕港浮游动物群落特征及影响因子

根据沙埕港2006年1月和4月冬、春季2个航次的调查资料,分析了浮游动物种类组成、优势种、丰度和生物多样性的季节变化,并利用PRIMER统计软件中的PCA、BIOENV和RELATE程序探讨了浮游动物与环境因子的关系.经鉴定共有浮游动物77个种类(含浮游幼虫15类 ).中华哲水蚤(Calanus sinicus)和真刺唇角水蚤(Labidocera euchaeta)是2个航次共有的优势种,桡足幼体在2个航次中也都占优势.与1990年调查相比,浮游动物丰度大幅下降,浮游动物种类组成已发生了较大改变.沙埕港的浮游动物大致可分为河口型类群、沿岸型类群和近海型类群.冬季航次浮游动物的平均总丰度为20.6 ind/m3,春季航次为92. 2 ind/m3,低于福清湾浮游动物同期调查结果.浮游动物总丰度分布均为由湾口向湾顶递减的趋势.春季的浮游动物总丰度和香农-威纳多样性指数均高于冬季,冬季浮游动物生物多样性呈现湾中、西部高,湾口、湾顶低的趋势,而春季浮游动物多样性分布比较均匀.经统计分析,影响冬季和春季浮游动物分布的主要环境因子为盐度、温度.     

大亚湾及其邻近海域冬季温度、盐度的分布及日变化特征

根据2018年1月冬季航次的水文实测资料,详细分析了大亚湾海水温度(T)、盐度(S)的分布特征。整体而言,观测海区海表相对于海底具有高温低盐的特征;同时,无论是表层还是近底层,大亚湾湾内的海水相对于湾外都呈现高温低盐的特征。观测期间,应是受到大亚湾核电站温排水的影响,湾内西侧存在一个高温中心。盐度的差异在近底层更加明显,低盐中心位于大亚湾的湾顶和大亚湾的中部海域,而高盐中心则主要分布于湾口西侧及惠东以东附近海域。太阳辐射和潮流变化是影响大亚湾温度、盐度变化的两大重要因素。其中,太阳辐射的影响主要局限于表层3~4 m,对近底层海水的影响较小;其加热效应使湾内和湾口附近的表层海水都表现出明显的昼夜变化。由潮汐和温度、盐度的对应关系可知,潮流对湾内温度、盐度的影响较大,而对湾外温度、盐度的影响较小。     

2009年夏季胶州湾同步调查浮游植物群落结构特征

根据2009年8月17日14:00胶州湾21个大面站的同步调查资料,研究了夏季胶州湾浮游植物群落结构特征。共检出浮游植物3门35属65种,平均细胞丰度为77.2cell/m L,硅藻在物种数量和细胞丰度上占绝对优势。主要优势种为中肋骨条藻Skeletonema costatum和大洋角管藻Cerataulina pelagica。夏季胶州湾浮游植物表层细胞丰度的平面分布趋势为北部高于南部,垂直分布趋势为从表层至底层细胞丰度明显降低。表层浮游植物的Shannon-Wiener指数和均匀度指数的分布显示胶州湾西北部和南部浮游植物群落结构更加稳定。与环境因子的相关分析表明浮游植物细胞丰度与盐度呈负相关,与硝酸盐、亚硝酸盐、磷酸盐呈正相关。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Assessing the Validity of Seismo-Acoustic Predictor Equations for Obtaining Seabed and Sub-Surface Sediment Physical Properties

The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.     

A Review of Some Environmental Issues Affecting Marine Mining

This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested.     

Increased Particle Fluxes at the INDEX Site Attributable to Simulated Benthic Disturbance

Particle fluxes were measured 7 m above the sea bottom during the predisturbance, disturbance, and postdisturbance periods by using time series sediment traps attached to seven deep-sea moorings deployed in the INDEX experiment site in the Central Indian Basin. The predisturbance particle fluxes varied between 22.3 to 55.1 mg m -2 day -1 . Increased and variable particle fluxes were recorded by the sediment traps during the disturbance period. The increase observed was 0.5 to 4 times more than the background predisturbance fluxes. The increases in particle fluxes (~4 times) recorded by the sediment trap located in the southwestern direction (DMS-1) were the greatest, which could be the result of preferential movement of resuspended particles generated during the deep-sea benthic disturbance along the general current direction prevailing in this area during the experimental period. Also, the traps located closer to the disturbance area recorded greater fluxes than did the traps far away, across the Deep Sea Sediment Resuspension System path. This variability in recorded particle fluxes by the traps around the disturbance area clearly indicates that physical characteristics such as grain size and density of the resuspended particles produced during the disturbance had an important effect on particle movement. The postdisturbance measurements during ~5 days showed a reduction in particle fluxes of ~50%, indicating rapid particle settlement.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Determination of In Situ Sediment Shear Strength from Advanced Piston Corer Pullout Forces

The advanced piston corer (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments.     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

Interannual Sea Level Variations and Annual Tides in the Northwestern Pacific

Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.     

Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects

The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.