Thermolability of dehydrogenases from a psychrophilic marine bacterium

The heat lability of succinic dehydrogenase, malic dehydrogenase and lactate dehydrogenase in cell-free extracts ofVibrio marinus MP-1 grown at 15C was compared for the tris buffer suspended enzymes and the NaCl suspended enzymes. The tris buffer suspended enzymes lost 50 percent activity at low thermal exposure between 5C and 27C. No activity was evident on thermal exposure between 32C and 39C. The enzyme extracts suspended in NaCl at 36 % were more heat stable, losing 50 percent activity after exposure between 16C and 40C. The enzymes lost all activity on thermal exposure between 33C and 42C in the presence of NaCl.     

Studies on the dissolution of diatomaceous silica as a function of heating

Diatomaceous silica, pretreated at various temperatures between 125 and 900C, was subjected to dissolution experiments and was analyzed using X-ray diffraction and infrared spectra. The diatomaceous silica preheated up to 900C, dissolved throughly into the solution of 2 per cent Na₂CO₃. On the digestion in the solution of 2 per cent Na₂SO₄, the dissolution of the silica decreased as the pretreating temperature increase; the degree of dissolution was different for various species. According to infrared absorption studies, the diatomaceous silica pretreated at a temperature below 500C preserved the reversibility of dehydration-rehydration almost qualitatively, while heating above 500C the samples lost the reversibility. Deformation of some kinks of diatomaceous silica pretreated above 500C, was percepted on infrared absorption spectra around 1,150 cm^–1. The samples pretreated up to 900 C, however, did not show such an internal structural change as could be identified by the X-ray diffraction analysis. The content of Al and Fe in diatomaceous silica was low and have not much difference in quality among diatom species. From these experimental results, it was adequately understandable that diatomaceous silica, which has a metastable character, is variable in the silica forms among species and its process and rate of transformation of internal structure and/or surface sites by the treatment at given temperatures also show differences.     

Detailed oceanic structure in the vicinity of the shoal Kokusho-sone

The detailed oceanic structure was observed near the shoal Kokusho-sone (3000N, 12830E), which is located near the axis of the Kuroshio in the East China Sea. The detailed temperature cross-sections along the meridian 12830E, which passes over the shoal, strongly suggest that upwelling is forced along the north slope of the shoal. The behavior of the coastal waters near the Kuroshio front and of the cold water belt along the north-western side of the Kuroshio are also discussed.     

Heat balance of the upper ocean under a land and sea breeze in Sagami Bay in summer

The heat balance of the upper ocean under a land and sea breeze was investigated based on observations of sea water temperature in the upper 300 m layer and heat flux across the sea surface at a fixed station in Sagami Bay (3510N, 13925E) during two periods of two days in August 1980 and three days in August 1981. During both periods, a typical land and sea breeze of 4–6 m sec^–1 at maximum prevailed in the observation area. Large diurnal variation of sea surface temperature with a maximum peak around noon LST was observed during both periods (the daily value of the range was 0.9C and 2.5C in 1980, and 1.2C, 1.5C and 1.7C in 1981). It was found that these large temperature variations were caused by diurnal variation of the wind speed which dropped to 0–3 m sec^–1 at noon when the strongest insolation (–270 Wm^–2) penetrated the sea and at midnight in association with alternations of the land breeze and the sea breeze. On the other hand, vertical mixing of the sea water caused by the wind stress and/or convection due to cooling at night extended down only to the surface 10 m layer. Horizontal heat advection was negligibly small. Therefore the local time change of the heat content in the upper 10 m water column was affected mainly by the heat flux across the air-sea interface which was estimated from data on radiation fluxes measured directly on board and latent and sensible heat fluxes calculated by the aerodynamic bulk method. The water temperature below the 10 m layer also varied with time and the temperature variation in the thermocline (20–50 m depth) was frequently larger than that of the sea surface temperature. However, the variation in the upper 10 m layer was little influenced by that below the layer.     

The heterotrophic activity for amino acids,glucose and acetate in Antarctic waters

The kinetic approach ofWright andHobbie (1966) was used to determine the heterotrophic potential of Antarctic waters. Variations with depth and geographical location are discussed. Temperature studies indicate the psychrophilic nature of the microbial population. In samples having environmental temperatures of approximately –1C, significant activities were recorded at the lowest incubation temperatures tested (–3C). Activities increased with increasing incubation temperatures up to +3C but were somewhat depressed at +10C and above. By testing a wide range of substrates, qualitative differences were noted in the microbial populations of different areas.Highest rates of uptake were found in the euphotic zone whereas V_max values of samples from depths of 500 m and below were in most cases unmeasurable.Published as Technical Paper No. 4259, Oregon Agricultural Experiment Station.     

Phase velocity of semi-diurnal internal waves at Ocean Weather Station T

Phase velocity of semi-diurnal internal waves is determined from differences between phases at three stations which were situated to form a triangle in the vicinity of sta. T (29N, 135E). The wave phases are estimated from temporal variations in depths of isotherms obtained from serial measurements of vertical temperature profiles at these stations. The measurements were carried out in cooperative operation of two vessels, the R. V.Tansei-maru and theNojima, during the period from 30 July to 1 August 1965. Wave propagation with the speed of about 2 m/s in the direction from east to west is obtained as an average over several isotherms of temperature from 19C to 23C. The area of measurement is to the west of Izu-Mariana ridge and the distance from the ridge to the station is about 500 km, which would be about 5 times as large as the wave length of the internal waves under consideration, and so it is possible to suppose that the internal waves observed generated at the ridge and propagated to the area without being subjected to serious refraction, scattering, reflection and decay.     

Rates of net assimilation and respiration of amino acids by marine bacteria

The selectivity of amino acid assimilation by marine bacteria was examined using seven kinds of¹⁴C-amino acids and the acid hydrolysate of¹⁴C-labelled proteins. It was found that the net assimilation and respiration by marine bacteria followed MICHAELIS-MENTEN kinetics for all of amino acids used in our experiments. Maximum velocities of amino acids were 0.01 to 0.19g carbon/hour per 2×10⁷ cells for net assimilation and less than 0.18g carbon/hour per 2×10⁷ cells for respiration at 20C. The velocity of gross assimilation was found with the following order: phenylalanine>valine, glutamic acid>serine, arginine>tryptophan>glycine. The assimilation velocities of amino acids in these laboratory works showed almost the same order as those in field experiments. The assimilation velocity of an amino acid was influenced by coexisting another amino acids or glucose. The assimilation velocity in lower substrate range of amino acids was directly proportional to the number of bacterial cells in the range from 6×10² to 3×10⁴ cells per ml. No linear relation between the assimilation velocity of amino acids and reciprocal of absolute temperature was found, but a marked bending was observed at 15 to 20C. The velocity at the optimum temperature was three to six times of that at 5C.     

Indirect evidence of deep flow east of a North Pacific seamount

East-west vertical sections centered on Kinmei Seamount (35N, 172E) of potential temperature, salinity, and oxygen, based on recent standard hydrographic measurements, and vertical profiles ofin situ temperature and density ( ₄) east of the seamount, based on STD data, show contrasting variability in the deep water characteristics east and west of the seamount. These data are consistent with horizontal variations in water mass properties (temperature-salinity and temperature-oxygen curves) east of the seamount between 2,600–3,900 m but not west of the seamount. East of the seamount on surfaces of constant potential temperature (or density) salinity and oxygen are higher at a station 200 km from the seamount than at stations on either side. From these relations and from known deep water properties of the North Pacific, it is suggested that east of the seamount, between 2,600–3,900 m, the observations are consistent with northward flow, which is interpreted to be a western boundary current with an east-west scale of about 300 km.     

Differential sorption of organic matter by various sized sediment particles in recent sediment from the bering sea

A recent unconsolidated marine sediment collected from the Aleutian basin of the Bering Sea (5702.9N, 17657.4W) was separated into eight size fractions by sieving. Each of the fractions was analyzed for density, organic carbon, total nitrogen and various organic materials. A detailed analysis of the correlation of organic carbon and total nitrogen to the mean diameter of the particles separated, indicated that the surface sorption processes of organic matter were the determinative factor controlling the concentration of organic carbon and total nitrogen on silt and clay particles of the sediments.Selective sorption of amino acids was found to occur in the 8–20 and 5–8m. fractions of the sediment. This is assumed to be due to their surface active property.     

Vertical distribution of heavy metals and its seasonal variations in Beppu Bay

From July to November, the thermocline which has strong temperature gradient (0.7C m^–1) is formed in the bottom water of Beppu Bay, and it prevents the downward mixing of surface water. This has caused the bottom water of the basin to become depleted in oxygen, and in November the bottom water below about 60 m depth becomes anoxic. Accordingly manganese and iron are reduced and more soluble under the anoxic condition, those concentrations are high relative to surface water, and the maximums are 1,240g l^–1 and 80g l^–1. Under the anoxic condition, the flux of dissolved manganese from the sediment is about 10g cm^–2 day^–1.     

AOU as indicator of water-flow direction in the central North Pacific

Salinity, preformed phosphate and AOU distribution on the sigma-t surfaces of 26.8 and 27.3 of the central North Pacific Ocean, as well as the topography of these density surfaces, were studied. The direction of water flow suggested by the AOU distribution on these density surfaces was compared to that indicated by the acceleration potential contours on the _t =125 cl/ton and _t =80cl/ton surfaces drawn by Joseph L.Reid, Jr. The disagreements were explained in terms of mixing and possible gradients of primary production at the sea surface. On the 26.8 sigma-t surface a southward flow connecting the westward flow south of the Aleutian chain and the eastward flow farther south, between 175E and 180 is suggested by the AOU distribution but is not implied by the acceleration potential contours. If the circulation pattern at this density surface is similar to that at the sea surface, this southward flow is very likely to be real.     

Intrusive Stratification of the Gulf-Stream Frontal Zone According to the Results of Investigations Performed during Cruise 43 of the R/V Akademik Vernadskii

We analyze the data of investigation of the intrusive structure of the Gulf-Stream frontal zone obtained in making frequent drift sections with the help of an MGI-8102 probing complex, study the regularities of variation of temperature, salinity, and density along separate intrusions, and present a series of results connected with the specific features of initiation and development of intrusions and the types of exchange processes determining their transformations. It is shown that the T-diagrams of all intrusions are well separated into segments with different slopes. Moreover, by comparing the slopes and locations of these segments with each other and with the T-diagrams of the Gulf Stream and slope waters, we can fairly reliably attribute the corresponding segments of intrusions to one of the following four types: initial dynamic folds of the frontal zone, layers of domestic water separating the intrusive segments of foreign water from each other, intrusive segments characterized by the penetration of ambient water, and segments not intrusive initially but getting the required slope as a result of interaction with upper and lower intrusive segments. For segments of the last two types, it is possible to specify the predominant type of exchange.     

Observed relationship between the drag coefficient,Cd, and stability parameter, (−z/L)

Momentum and heat flux were measured with a sonic anemometer at the Marine Observation Tower in the port of ItÔ. Under unstable conditions (T _w -T _a =3C4C), using the eddy correlation method, results show thatCd=(1.2±0.3)×10^–3 andCh=(1.5±0.3)×10^–3 at 5.5 m above mean sea level except for the case of weak winds.An unexpected relationship betweenCd and (–z/L) was observed, that is,Cd decreases as (–z/L) increases. If roughness variation over the sea is taken into account, we can explain the decrease in the range of (–z/L) less than 1, but not in the range greater than 1. This is due to a strong instability effect and the change of roughness class, from moderately rough to smooth.     

Classification of “siome”, streaks and fronts

Siome, which was firstly defined by Uda (1938); is a line of convergence on the sea surface. There are many kinds of siome in the sea. I propose in this paper that siome should be classified into streak and front. Streak is defined as the convergence within the same water mass and front the convergence between two different water masses. Streaks and fronts are classified into more details on the basis of their mechanisms of generation. The proposed classification will be useful for understanding the dynamics of siome and should help to promote interdisciplinary studies around siome regions.     

Variation of water system distribution in the Sanriku Coastal Area

We analyzed hydrographic data obtained monthly by the Iwate Prefectural Fisheries Laboratory during 1977–1981. Our attention was focussed on the classification of waters based on the Temperature-Salinity (T-S) scatter diagram analyses, and on the extraction of the mean state of their distribution for each season. The water system was defined as all waters occupying some specific region on the T-S plane, and the mean state for each season was expressed by the distribution of the mode water systems (i.e., the most commonly occuring water system).Because of the coexistence of the Tsugaru Warm Current, the Oyashio and the Kuroshio, and their large seasonal variability, the sea waters had a wide variety of T-S combinations. The detailed T-S scatter diagram analyses allowed us to classify the waters into six water systems. A time-longitude diagram of water systems and the distribution of mode water systems are presented. The latter showed clear seasonal variations of the Tsugaru Warm Current and the Oyashio.Though the water system analysis was successfully able to present the distribution of the various waters in the Sanriku Coastal Area and to clearly extract the mean state of sea conditions, it was found that the classification of water systems on the T-S plane was not complete in all cases for this area.     

Observations of upwelling around the Izu Peninsula,Japan: May 1982

Hydrographic observations between the Izu Peninsula and Oshima Island, Japan, in May 1982, showed upwelling around the tip of the Peninsula and possibly also in the lee of Oshima Island. The upwelling introduced water as cold as 18 C with nitrate concentrations of 3M to the surface. Temperature/salinity analyses indicated that the upwelled water was of Kuroshio characteristics. Slightly fresher water was advected out of Sagami Bay in a coastal counter current.Contribution number 470 from the Shimoda Marine Research Center, University of Tsukuba.     

Petrography and stable isotope aspects of cold-vent activity imprinted on Miocene-age “calcari aLucina” from Tuscan and Romagna Apennines,Italy

Over 20 occurrences of discontinuous limestone blocks, locally called calcari aLucina, were mapped in the Tuscan—Romagna region of the northern Italian Apennines. The limestones, consisting of a variable mixture of authigenic carbonates (calcite, dolomite, and aragonite), sulfides (primarily pyrite), and allogenic silicates, occur in association with turbidite and hemipelagite units that were deposited in foredeep basins during early to late Miocene times. The limestone blocks are interpreted to represent relicts of carbonate buildups formed around methane-rich fluid vents on the basis of their (1) striking petrographic similarities to carbonates from cold vents in the modern oceans; (2) unique chemosynthetic-like fauna, and (3) anomalously negative ¹³C values ( ¹³C = – 16 to – 58 PDB). The contemporaneous tectonism of the Apennine orogeny is likely to be the primary cause for the expulsion of the methane-rich fluids to the seabed in a manner analogous to the fluid-flow processes occurring at modern accretionary prisms.     

Kinetics of nitrate and ammonium uptake by the natural populations of marine phytoplankton in the surface water of the Oyashio region during spring and summer

The maximum uptake rate (_max) and affinity constant (K _s) for nitrate and ammonium were estimated in the surface water of offshore Oyashio in May (spring) and September (summer), 1990. The average _max/Chl.a for ammonium was 2.1 times larger than that of nitrate in both seasons. The average _max/Chl.a for both nitrogens were 3.5 times larger in summer than in spring. Water temperature and size composition of phytoplankton population were related to the seasonal difference in the _max/Chl.a. Phytoplankton population showed high affinity for both nitrogens in the spring and summer. In addition, the contribution of new production to total production was estimated by _max[_max–No₃/(_max–NO₃+_max–NH₄)]. The spring value was in the range of 0.26 to 0.45 (mean±SD=0.35±0.092), and the values in spring bloom were especially a little over 0.4. The summer value was in the range of 0.30 to 0.37 (0.34±0.04).     

The statistical distributions for the elevation,velocity and acceleration of the surface of wind waves

Water surface elevations(t), vertical surface velocities and vertical surface acceleration of wind-generated waves have been measured in a laboratory wind wave channel by using resistance-type wave gauges combined with an electronic differentiation circuits. Probability distributions of the values of(t), , and have been determined from the wave records.In an initial stage of wave generation,i.e., when wind waves are generated at short fetches and low wind speeds, the observed distributions for(t), and are appreciately good fit to the distributions given by successive sum of a Gram-Charlier series, which has been derived following the formulation ofLonguet-Higgins (1963), by taking the weakly nonlinear effect into account.However, when wind waves develop with increasing wind speeds and fetches, the observed distributions deviate gradually from the Gram-Charlier series. Particularly, the deviations are remarkable for the distribution of .When the wind speed increases, the observed distributions of(t), and show the following characteristics: (i) the skewnesses of the distributions of(t) and decrease slightly, (ii) the skewness of changes, at some wind speed, from positive small values to relatively large negative values, (iii) the kurtosis of the distribution of(t) decreases slightly but that of increases slightly and these characteristics seem to depend not so much on fetches, (iv) the kurtosis of the distribution of increases rapidly.     

Parameterization of the dispersion relation of internal waves using the data of hydrological soundings in the Tropical Atlantic

Dependences have been determined which connect the parameters of the dispersion relation of the lowest mode of internal waves with the integral characteristics of the seasonal thermocline when 10 min30 min, 20 mh150 m, and 0·4 m²/s² Q5·2 m²/s².Translated by Mikhail M. Trufanov.