温度、光照对小环藻生长和叶绿素a含量的影响

用单因子和正交实验方法研究了温度和光照对小环藻生长和叶绿素a含量的影响。结果表明,温度对小环藻生长和叶绿素a含量有极显著影响(P<0.01),当温度在30~40℃时比增长率和叶绿素a含量分别为0.37~0.38 d-1和1 084.4~1 214.2μg.L-1,均显著高于其他温度组。光照对小环藻生长和叶绿素a含量有极显著影响(P<0.01),当光照强度从29.25~146.26μmol.m-2.s-1时比增长率最高,为0.30~0.32 d-1;而叶绿素a含量则在9.75~58.50μmol.m-2.s-1时最高,达709.7~731.8μg.L-1,极显著高于其他照度组。正交实验结果表明:在35℃、87.75μmol.m-2.s-1条件下种群增长最快,比增长率为0.4402 d-1;在30℃、29.25μmol.m-2.s-1时,叶绿素a含量最高,为1 746.6μg.L-1。     

珠江磨刀门河口枯季叶绿素a环境因子驱动分析

【目的】分析珠江磨刀门河口浮游植物叶绿素a及其驱动因子的潮周期变化,并揭示叶绿素a的环境影响机制。【方法】基于珠江磨刀门河口2017年枯季(1月13-20日)水文水质多要素同步观测数据,采用主成分分析(PCA)和结构方程模型(SEM)确定浮游植物叶绿素a与多个环境因子的关系并量化各因子的驱动作用。【结果】磨刀门水道浮游植物叶绿素a浓度变化范围为0.77～10.92μg/L,口门处变化为0.70～9.01μg/L,磨刀门河口动力驱动下浮游植物叶绿素a的潮周期差异显著。盐度、含沙量和氮磷营养盐亦随潮汐涨落差异显著,而水温无明显潮周期变化。【结论】外海水团对氮营养盐的稀释混合作用强于磷营养盐,而悬浮泥沙对磷营养盐的吸附作用强于氮营养盐。与氮营养盐相比,溶解态磷相对不足,限制磨刀门河口浮游植物的生长。径流(营养盐、悬沙等为代表)、潮流(盐度为代表)对河口浮游植物的影响机制不同,其中氮磷营养盐和水温起着直接作用,而悬沙和盐度间接影响浮游植物叶绿素a的变化。     

秋季雷州半岛近海营养盐和叶绿素a浓度空间分布及其相互关系

【目的】研究秋季雷州半岛海域东西部水体营养盐和叶绿素a(Chl-a)空间变化特征及其相互关系。【方法】根据2017年10月(秋季)的现场调查资料,分别采用镉铜柱还原-重氮偶氮法、重氮偶氮法和硅钼蓝法测定硝酸盐、亚硝酸盐、磷酸盐和硅酸盐,以及萃取荧光法测定Chl-a浓度,深入探讨雷州半岛Chl-a浓度空间变化的调控机制。【结果】2017年秋季,研究区域近岸Chl-a及营养盐浓度大致呈现近岸高离岸低的分布特点;雷州半岛东部海域与西部海域相比,Chl-a浓度、DIN、Si O32-,nDIN/nP比较高,而nSi/nDIN和PO43-在西部略高于东部;营养盐对浮游植物生长的可能限制因子分析结果,在雷州半岛东西部环境因子对Chl-a浓度的影响程度不同,其中盐度和亚硝酸盐是影响Chl-a浓度的主要因子。在浮游植物生长的限制因素方面,雷州半岛东部海域表现为明显的磷限制,西部海域表现为明显的氮限制。【结论】秋季调查海域营养盐和Chl-a结构变化主要受海域水文情况(河流径流,潮汐类型)和人类活动的影响。调查海域东部受到河流径流和不规则半日潮的影响,营养盐、Chl-a浓度以及营养盐比值(PO43-和nSi/nDIN除外)都比较高。西部海域由于河流径流量小、人类活动较少以及受到规则的全日潮的影响,营养盐、Chl-a浓度以及营养盐比值含量较低且分布较均匀。     

湖光岩玛珥湖叶绿素a与水质因子的相关分析及富营养化评价

2013年1~12月对湖光岩玛珥湖水体的叶绿素a和水质因子进行周年的逐月监测,探讨叶绿素a与水质因子的相关关系,并应用修正的卡尔森营养状态指数(TSI)对该湖泊的营养状态进行分析评价。结果表明,湖光岩玛珥湖水体叶绿素a含量的变化范围为1.08~4.22μg·L-1。氮磷比年均值为20.9,磷为营养限制因子,氮、磷共同限制浮游植物的生长。影响叶绿素a含量的主动因子与叶绿素a之间的Pearson相关系数大小关系为:r(NH4+-N)r(DO)r(NO2--N)r(TP)r(TN)r(NO3--N),其中NH4+-N与叶绿素a含量呈极显著正相关,r(NH4+-N)=0.907,NH4+-N为调控该湖泊浮游植物生长最关键的水质因子。水体TSI值的全年变化范围为34.6~48.0,其中1、2、3月的TSI37,属贫营养型;而其他月份为38TSI53,属中营养型;从全年分布来看,湖光岩玛珥湖属贫-中营养型。     

温度、盐度和照度对北方娄氏藻生长和叶绿素a含量的影响

通过单因子与正交实验研究不同温度、盐度和照度对北方娄氏藻(Lauderia borealis)生长和叶绿素a含量的影响。结果表明:不同温度、盐度和照度对北方娄氏藻的比增长率μ和叶绿素a含量影响有统计学意义(P0.05)。当温度为20~30℃,盐度为25~35,照度为2 500~3 500 lx时,北方娄氏藻比增长率较大,叶绿素a含量较高,适宜北方娄氏藻的生长。温度和盐度分别是影响北方娄氏藻生长速率和叶绿素a含量的最主要因素,温度25℃、盐度35、照度2 500~3 000 lx是北方娄氏藻生长的最优组合条件。     

琼东海域冬季、夏季营养盐结构特征及其对浮游植物生长的影响

研究了琼东海域冬季(2012年12月)和夏季(2013年8月)表层水体营养盐结构特征和营养盐对浮游植物生长的限制情况。结果表明:2012年冬季,调查海域除了北部站位DIN、Si O32-浓度和nDIN/nP、nSi/nP比值较高之外,其余站位营养盐浓度和比值整体比较低且分布比较均匀,这是由于北部站位受到了具有高浓度DIN、Si O32-和低浓度PO43-的广东沿岸流的影响;2013年夏季,由于夏季河流径流量大,琼东近岸海域受到具有低浓度DIN、PO43-和高浓度Si O32-特征的河流影响,同时夏季近岸生物量增大和层化作用增强,因此DIN和PO43-浓度分布比较均匀,Si O32-浓度和nSi/nDIN、nSi/nP比值呈近岸高离岸低的分布特点。进一步分析该海域营养盐对浮游植物生长的可能限制因子发现,表层水体冬季呈现明显的硅限制,其限制因子几率为58.83%,夏季呈现氮-硅协同限制的可能性。由于生物利用、季节性的河径流量、广东沿岸流以及层化作用的影响,使得营养盐限制因子由冬季的硅限制转变为夏季的氮和硅共同限制。     

珠江口海域营养盐和叶绿素a的时空分布特征

【目的】研究珠江口海域营养物和叶绿素a的时空分布特征。【方法】根据2015年5月(春季)、8月(夏季)和10月(秋季)3个季节的现场调查资料,通过对3个季节浮游植物Chl-a以及包括氮磷比在内的营养结构进行综合分析,深入探讨珠江口海域浮游植物Chl-a的时空变化调控机制。【结果】珠江口海域各季节营养盐呈高氮低磷分布,3个季节N︰P均值49.6。叶绿素a季节差异明显,夏季叶绿素a含量是春季的2倍、秋季的9倍。【结论】春季浮游植物生长受温度影响较大,夏、秋两季由于外海水的入侵导致磷限制比较显著;DIN能满足各季节浮游植物生长的需要,珠江口海域浮游植物生长对于磷营养盐的响应要优于氮营养盐。     

夏季珠江口水域营养盐分布特征及其富营养化评价

【目的】研究夏季珠江口水域氮、磷营养盐的时空变化特征及其富营养化水平。【方法】基于珠江口水域2006-2010年夏季水文水质多要素观测资料,采用Pearson相关分析法,分析水文泥沙对氮、磷等营养盐的的驱动作用,评价该水域富营养化状况并阐明其主要环境问题。【结果】珠江口水域环境因子区域分布差异显著,其中营养盐(氮、磷等)和悬浮泥沙均表现为由口门向口外递减,而盐度则表现为相反的趋势。无机氮以硝氮为主,亚硝氮次之,氨氮含量最低。【结论】径流输入、盐淡水混合稀释作用和悬浮泥沙的吸附/解吸作用共同影响着氮、磷营养盐的分布。氮磷比值分析说明,珠江口浮游植物生长主要表现为潜在性的磷限制。珠江口水域海洋环境重度及严重富营养化(E 5)主要集中在口门至内伶仃岛海域,主要环境问题为水质污染,且氮、磷营养盐和溶解氧为主要贡献因子。     

青岛鳌山湾海区营养结构分析与营养状况评价

根据2004年6月中旬在鳌山湾海区所取得的底层海水营养盐资料,分析了青岛鳌山湾海区营养盐的平面分布特征;运用绝对限制法则和相对限制法则确定了鳌山湾海区的营养盐限制因子;采用营养状态综合指数法和分类分级富营养化评价模式对鳌山湾海域的营养状况进行了评价。结果表明:鳌山湾营养盐限制因子为磷(P),该海区由缺氮转变为缺磷,营养盐结构发生了根本性变化,且该海域无富营养化现象,水质较好。     

鱼汁、海泥抽出液和人尿对小环藻生长的影响

在室内条件下,通过单因子实验和正交实验的方法,研究了鱼汁、海泥抽出液和人尿对小环藻(Cyclotella sp.)种群生长的影响。结果表明,350 mL的培养体积下,当鱼汁添加量为2~3 mL时,小环藻生长效果最好,相对生长率(K)较对照组升高了33.3%,平均倍增时间(G)降低了34.9%,但随着浓度升高,藻细胞开始出现死亡。海泥抽出液添加量为20 mL时,K值最大,较对照组增加35.6%,G值下降了25.15%。人尿添加量为10 mL时,K值最大,较对照组增加17.9%,G值下降了15.77%。统计分析表明,鱼汁、海泥抽出液和人尿对小环藻实验种群的生长均有极显著影响。海泥抽出液对小环藻影响大,其次是鱼汁和人尿。正交实验表明,在鱼汁、海泥抽出液和人尿的添加量分别为2.5、15.0和1.0 mL的组合实验组,小环藻的生长效果最好。     

徐闻珊瑚礁保护区营养盐时空分布特征

2006年8月(夏季)、2006年11月(秋季)、2007年2月(冬季)和2007年4月(春季)在徐闻珊瑚礁自然保护区灯楼角至流沙湾近岸海域调查徐闻珊瑚礁保护区的营养盐变化及空间分布特征。结果表明:徐闻珊瑚礁保护区水域溶解态无机氮以NO3--N为主,其含量超过总溶解无机氮的50%;各站点NO2--N含量相对较低,冬季NO3--N和NH4+-N含量均高于其它季节;无机磷含量在0mg/L～0.030mg/L之间变化;活性硅含量表现为夏秋季节高、冬春季节低;表层水体硝酸盐氮/无机磷原子比值(N/P)夏季较低,不存在无机磷受限情况,而冬季N/P整体较高,此时水体主要受无机磷限制。     

Nutrients in seawater and interstitial water of the sediments in the Huanghe River estuarine area (November)

The investigation shows that the concentrations of nutrients are high in estuarine and coastal waters and low in offshore waters. The concentration of nitrate in estuaries is controlled through a physical mixing process and is also affected by biotic activity. The annual transport of total inorganic nitrogen and dissolved phosphate-phosphorus from the Huanghe River water to the sea is about 8.45 ×10⁴ and 1.45×10³ tons respectively. The distributions of inorganic nitrogen and silicate in interstitial water of surface sediments are similar to those in surface and bottom seawater. Their contents in interstitial water are 227–552 μmol/l (average375) for ammonia, 0.31–9.0 μmol/l (average 1.6) for nitrite, 0–41 μmol/l (average6.0) for nitrate, and 41–139 μmol/l (average 77) for silicate. The average concentrations of phosphate in the surveyed area are 0.64 μmol/l for seawater and 1.2 μmol/l for interstitial water. A cycle of phosphate in the estuary is also suggested in this paper. Contribution No. 1434 from Institute of Oceanology, Academia Sinica.     

EFFECT OF NITROGEN AND PHOSPHORUS ON THE GROWTH OF A RED TIDE DINOFLAGELLATE SCRIPPSIELLA TROCHOIDEA (STEIN) LOEBLICH III

INTRODUCTIONRedtidecanadverselyaffectcoastalfisheriesbycausingthedeathofculturedfishandshrimporproducingtoxinsthatcanbeaccumulatedthroughfoodchains (Anderson ,1 989) .Phytoplanktonisoftenthemaincomponentofmanyredtides.AlongthecoastofJiaozhouBay ,P .R .China,theredtideismainlyformedbyadinoflagellate ,Scrippdiellatrochoidea ,duringsummer (JulytoSep tember)andthecelldensitycanreach 9.98× 1 0 7cells ml (Zou ,unpublisheddata) .Manyresearcherssuggestedthatredtideofphytoplanktonmightresultf…     

Effects of Nutritional Factors on the Growth and Heterotrophic Eicosapentaenoic Acid Production of Diatom Nitzschia laevis

The effects of several nutritional factors on the growth and eicosapentaenoic acid (EPA) production of diatom Nitzschia laevis were studied. 4 LDM (quadrupled concentration of the nutrient salt) was the optimal concentration of nutrient salt for the growth and EPA production ofN. laevis. The growth ofN. laevis was inhibited when the glucose concentration was either lower than 10gL-1 or higher than 15gL-1. Both sodium nitrate and urea were good nitrogen sources for the growth and EPA production, while ammonium chloride seriously decreased the dry cell weight (DW) and the EPA content. Silicate seriously influenced the growth of N. laevis. The maximum DW of 2.34gL-1 was obtained in the presence of 150 mgL-1 Na2SiO3·9H2O. The EPA content remained almost the same when the silicate concentration was lower than 150 mgL-1; however, higher silicate concentrations resulted in a steady de- crease of EPA content. Low medium salinity (≤29) did not seem to influence the DW of N. laevis, and high salinity resulted in a decrease of DW. The highest EPA content (4.08%) and yield (110 mgL-1) were observed at the salinity of 36 and 29, respectively.     

Distributions of inorganic nutrients in the bohai sea of china

1　Introduction　TheBohaiSeaislocatedinthenorthernChinawithlongitudesofbetween 117°38′Eand 12 2°31′Eandlat itudesofbetween 37°0 8′Nand 4 1°0 2′N .Itisashal lowseawithanaveragewaterdepthof 18m (LiuandZhang ,2 0 0 0 ) .Severalbigrivers ,suchastheLiaoheRiver,theHaiheRiverandtheYellowRiver ,findtheirwaysintotheBohaiSeaandtransportlargeamountofnutrientsandsuspendedmattersfromthecontinentintothesea (Zhangetal.,1994 ;Zhang ,1996 ) .Duringthelasttwodecades ,marineenviron mentintheBohai…     

BIOGEOCHEMICAL BEHAVIOR OF NUTRIENTS AND THEIR FLUXES IN THE MINJIANG RIVER ESTUARY

IMRODUcTlONThe577km1ongMinjiangRiver,thelongestriverinFujianProvinceofChina,hasa6O992km2drainagebasin.Thewaterdischargeislarge(meaninyearsis198Om/s).TheannualrunofffromtheMinjiangRiveris5.84xlOlom,WhichismorethanthatoftheHuangheRiver,andranksthirdintheannualrunoffofmaorChineseriversWhang,l994).SimdentnutrientSfromtheMinjiangRivertotheseaisanarisa-ryconditionforthefonnationandcontinuingexistenceoftheMindongFisheryGroundandtheMihahongFisheryGround.Inthendingareaofriverwaterwithseaw…     

Effect of nitrogen and phosphorus on the growth of a red tide dinoflagellateScrippsiella trochoidea (Stein) Loeblich III

Scrippsiella trochoidea (Stein) Loeblich III was grown in a nitrogen-or phosphorus-limited batch culture system in laboratory. Growth rates and cellular Chl-a were measured as functions of nitrate and phosphate concentrations. Growth rates were hyperbolic with both nitrate and phosphate concentration and fit the Monod equation. The minimum cell quota of nitrogen and phosphorus and then the optimum N∶P ratio ofS. trochoidea were estimated in this study. Measurement of phosphate concentration in Jiaozhou Bay suggest that phosphorus is the limiting factor ofS. trochoidea growth. Contribution No. 3679 from the Institute of Oceanology, Chinese Academy of Sciences. Project 39790110 supported by NSFC, the study aslo supported by the National Climbing Project B (PDBG-7-2) and partly supported by an MF grant from Hong Kong University of Science and Technology.     

培育密度、换水量和倒池培育对珠母贝浮游幼虫生长发育和存活的影响

以室内育苗池或玻璃缸养殖方式,研究了不同培育密度、换水量和倒池培育对珠母贝浮游幼虫生长发育和存活的影响。结果表明:当培育密度为0.8、1.2只/mL,直线铰合期时间分别为7.00、7.40d,日均生长为5.16、4.87μm,壳顶期时间分别为10.76d和10.77d,眼点期密度为0.58只·mL-1和0.60只·mL-1,差异不显著;日换水量分别为40%、60%、80%,直线铰合期时间、壳顶期时间、日均生长及眼点期密度差异不显著,日换水量40%能满足幼虫的生长和存活;壳顶初期进行倒池培育,能促进浮游幼虫的生长发育和提高其存活率。     

Forms and Balance of Nitrogen and Phosphorus in Cage Culture Waters in Guangdong Province, China

In order to approach the characteristics of nitrogen and phosphorus pollution caused by cage culture and the balance of nitrogen and phosphorus during the process of cage culture, a monitoring was conducted in Daya Bay of Guangdong Province, China from April 2002 to Jane 2003. The results show that the concentrations of total nitrogen (TN) in the waters at the sites with five and ten years of cage culture history are 1.8 and 2.3 times of that at control site respectively. Ammonium (NH3-N) is the main form of nitrogen in spring while nitrate ( NO3 -) in winter. The concentrations of TN, total dissolved nitrogen (TDN) and dissolved organic nitrogen (DON) are highest in autumn. The concentration of phosphorus increases with the increasing of the culturing time, among which phosphate ( PO 34-) increases most obviously. The concentrations of total phosphorus (TP) and total dissolved phosphorus (TDP) are highest in autumn. The nitrogen and phosphorus are accumulated significantly in the sediment of cage culture area. The model of N balance in the cage culture area: bait (70.62%) fry (0.28%) input by tide (14.8%) release from sediment (14.3%) = harvest of adult fish (12.07%) deposition into sediment (28.75%) output by tide (56.18%) others (3.00%). The model of P balance: bait (83.11%) fry (0.17%) input by tide (12.23%) release from sediment (4.49%) = harvest of adult fish (8.43%) deposition into sediment (48.59%) output by tide (41.94%) others (1.04%). In one fish growth year, the contents of nitrogen and phosphorus in harvest of adult fish are only 17.0% and 10.1% of the contents of nitrogen and phosphorus in fish bait and fry, wherein 83% of nitrogen and more than 89% of phosphorus in fish bait became marine pollutants.