胶州湾浮游植物粒级结构及其时空变化

基于2003—2010年间对胶州湾分粒级叶绿素a浓度的连续观测,系统研究了胶州湾浮游植物粒级结构的季节变化、年际变化及长期变化特征。结果表明,胶州湾表层浮游植物粒级组成以小型和微型浮游植物为主,其浓度由东北部和北部向湾中间及湾外逐渐递减。不同区域分粒级叶绿素a浓度的季节与年际变化规律相似。小型和微型浮游植物表现出明显的双峰型季节变化,小型浮游植物的高峰值通常出现在冬季,而微型浮游植物则出现在夏季。长期变化结果表明,冬季小型浮游植物所占比例自90年代起表现为增加的趋势,而夏季的变化规律与冬季相反,自1998年开始,小型浮游植物所占比例下降,微型浮游植物比例有所上升。春季和秋季小型和微型浮游植物的贡献率没有表现出明显的升高或降低趋势,但微微型浮游植物的贡献率在2000年之后显著低于2000年之前。统计分析结果表明,温度、营养盐浓度与结构是影响胶州湾浮游植物粒级结构变动的重要因素。     

春季海州湾临洪河口浮游植物粒级结构及环境因素

2017年4月作者对海州湾临洪河口海域16个站位的常规理化因子以及浮游植物粒级结构进行了采样和分析,结果表明:调查海域盐度和温度均值分别为35.2和11.2℃,均由近岸到外海逐渐增大;悬浮物质量浓度在3.4 mg/L～137.6 mg/L变化,均值为22.8 mg/L,由近岸到外海逐渐减少,水体逐渐清澈; pH均值为8.29,近岸低,外海高; DO均值为9.8 mg/L, COD在0.34 mg/L～6.55 mg/L变化,均值为3.42mg/L,DO和COD分布规律不明显;浮游植物粒级组成以微型与小型浮游植物为主,其叶绿素a质量浓度平均值与范围分别为1.48μg/L(0.68μg/L～3.13μg/L)、9.14μg/L(2.69μg/L～25.50μg/L),且呈现自沿岸河口向外海逐渐递减的趋势;而微微型浮游植物叶绿素a质量浓度平均值仅为0.27μg/L,且分布较不规律;小型浮游植物对浮游植物总生物量的贡献率最大,高达83.89%,微型及微微型浮游植物的贡献率分别为13.60%和2.52%;在相关性分析中,各项环境因子对小型浮游植物的分布有较大的影响,其中悬浮物、pH与小型浮游植物叶绿素a浓度表现出显著相关(P0.05),溶解氧、盐度与小型浮游植物叶绿素a质量浓度呈极显著相关(P0.01),微型、微微型浮游植物的分布与各项环境因子的相关性不明显。     

长江口邻近海域浮游植物粒级结构特征及其与环境的响应关系

浮游植物的分粒级研究是监测浮游植物特征的重要工具,对于深入了解浮游植物动态的作用也不容忽视。本文的研究目的在于揭示长江口邻近海域春秋季不同粒级的浮游植物分布动态,分析浮游植物粒级结构与环境因素以及浮游动物群落结构的关系。通过2010年春季和秋季对粒径分级叶绿素a浓度的现场调查研究发现：春季,浮游植物主要以微型浮游植物占优势;秋季,微微型浮游植物和微型浮游植物共同占优势。相关分析结果表明,温度和富营养化状况是影响微型和微微型浮游植物对总叶绿素a贡献的重要因素。浮游动物的摄食压力可能对小型浮游植物对总叶绿素a的贡献起着重要的作用。     

春季季风间期巽他陆架和马六甲海峡表层海水浮游植物群落结构研究

于2013年3-5月通过走航取样分别对巽他陆架和马六甲海峡表层海水浮游植物叶绿素a生物量和群落结构进行了观测和研究。结果表明:巽他陆架生物量较低,叶绿素a浓度平均值为(0.083±0.043)μg/L,爪哇海的SS4站位生物量最低,仅为0.014μg/L,浮游植物粒级组成上主要以Pico-级为优势,占80%以上;马六甲海峡自西北至东南存在明显的盐度梯度,在盐度最低的SM5站,叶绿素a生物量最高,达到1.080μg/L;马六甲海峡站位叶绿素a浓度平均值为(0.433±0.315)μg/L,同时浮游植物群落结构变动较大。在海峡西北的SM1-SM4站与巽他海峡类似,主要以聚球藻为优势类群,Pico-级浮游植物占60%~80%;在生物量最高的SM5站,同样以聚球藻为优势类群,而在海峡东南段的SM6和SM7站,虽然叶绿素a浓度相对于SM5略有降低,但仍明显高于其他马六甲海峡站位和巽他陆架站位,此两个站位硅藻比例明显升高,均可达20%以上。从优势类群生物量与环境因子和营养浓度的相关性可以看出,研究海区叶绿素a生物量与水体盐度呈现显著负相关(p0.050),说明陆源输入对研究海区生物量具有明显的影响。另外,硅藻生物量也与磷酸盐浓度(p0.050)和硅酸盐(p0.010)浓度均呈现显著正相关;聚球藻在浮游植物群落中的优势度会受到陆源营养盐输入的影响而降低,但仍然是整个研究区域最优势的浮游植物类群。     

渤海中部和渤海海峡及其邻近海域浮游植物粒级生物量的初步研究 Ⅰ.浮游植物粒级生物量的分布特征

对1998年9月和1999年4月在渤海(37°～41°N,117.5°～122.5°E)进行的两次生态系统综合外业调查,对蓬莱、长岛两地进行15个月的连续资料监测,对渤海调查区浮游植物粒级生物量的平面分布、垂直分布、昼夜变化和周年变化特征进行了研究.结果表明,秋季主要以小型浮游植物为主,然后依次为微型和微微型浮游植物;春季主要以微型浮游植物所占比重最高,其次为小型浮游植物,微微型浮游植物占比例最低.1998年秋季对断面1,2浮游植物粒级组分的垂直分布研究表明,在不同海区(调查区西部、南部、渤海中部和渤海海峡)的不同水层,浮游植物粒级生物量的分布有明显差异.潮汐对浮游植物粒级生物量的周日变化影响较大.蓬莱、长岛两地的连续调查资料表明,浮游植物各粒级组分除微微型浮游植物外1a有两个峰.网采浮游植物在4和7月有高峰,4月是主峰;小型网采浮游植物在6和11月有高峰,6月是主峰;微型浮游植物在4和11月有高峰,两峰值接近;微微型浮游植物在9月有高峰.调查区与其他海区浮游植物叶绿素a浓度粒级组分比较,表明小型浮游植物所占比重较大.     

南海北部东沙天然气水合物区浮游植物生物量和生产力粒级结构特征及其环境影响分析

本文讨论了2013年5月南海东沙天然气水合物区浮游植物生物量和生产力粒级结构特征及其环境影响因素。结果表明,研究海域表现出典型的低营养盐、低叶绿素a、低生产力特征,浮游植物叶绿素a和初级生产力具有明显的次表层最大值现象。东沙海域生物量和初级生产力粒级结构差异性显著,从生物量和生产力贡献度来看,表现为微微型浮游植物> 微型浮游植物> 小型浮游植物。生物量的垂直分布结果表明,春季不同粒级类群浮游植物在真光层内的分布存在明显不同,比如小型浮游植物在真光层内分布较均匀;微型浮游植物则主要分布于近表层或真光层中部,而微微型浮游植物则主要分布于真光层中部和底部。微微型浮游植物在纬度较低的热带贫营养海区之所以能够占主导优势,最主要的原因是其极小的细胞体积和较大的表面积使其有利于营养竞争。相关性分析表明,南海东沙浮游植物各粒级生物量与温度、pH显著正相关,与硅酸盐、磷酸盐显著负相关;浮游植物各粒级生产力与温度显著正相关,与盐度、磷酸盐显著负相关。磷酸盐含量是影响东沙海域浮游植物粒级结构差异的重要因素之一,同时,光辐照度和水体的真光层深度对东沙天然气水合物区不同粒径浮游植物的垂直分布起着更为重要的调控作用。     

2011 年春夏季黄、东海浮游植物粒级结构

通过2011年4月和8月利用"科学三号"考察船在黄、东海海域开展的春、夏季综合调查,研究了黄、东海浮游植物粒级结构的分布格局及其时空变动规律,探讨重要环境因子的变动对浮游植物粒级结构的影响。结果表明,春季表层水体中小型、微型和微微型粒级叶绿素a浓度的范围分别为0—4.36、0.02—2.27、0—2.66mg/m3,平均叶绿素a的浓度分别为0.56、0.31和0.14mg/m3,对叶绿素a总量的贡献率分别为55.4%、30.8%和13.8%。夏季表层由大至小3个粒级浮游植物叶绿素a浓度范围分别为:0—6.78、0—2.59、0—0.86mg/m3,平均叶绿素a含量依次为0.50、0.24和0.07mg/m3,对叶绿素a总量的贡献率分别为61.8%、30.1%和8.1%。春季小型浮游植物叶绿素a浓度的垂直分布较为均匀,微型和微微型浮游植物浓度随深度增加呈现逐渐下降趋势。夏季叶绿素a浓度出现明显分层现象,10m层以上小型和微型浮游植物浓度较高,10m层之下浓度迅速降低。微微型浮游植物浓度在不同水层都保持较低水平。受黄、东海不同季节水团影响而引起的温、盐以及营养盐分布格局的变化是影响黄、东海浮游植物粒级结构组成的重要因素。     

厦门岛西北海域浮游植物生物量粒级结构及其环境影响因素

文章利用2018年9—11月厦门岛西北海域的现场调查资料,在对海域水质污染状况和富营养化水平进行评价的基础上,分析并讨论浮游植物生物量粒级结构及其环境影响因素。研究结果表明:同安湾在11月处于富营养水平,属于中度或严重污染海域;九龙江口和西海域在9-11月均处于磷中等限制潜在性富营养或磷限制潜在性富营养水平,属于严重污染海域。同安湾和西海域浮游植物分粒级的生物量占比与九龙江口存在明显差异,前者由大到小依次为微型、小型、微微型,后者由大到小依次为小型、微型、微微型。小型浮游植物生物量与硝酸盐和亚硝酸盐、溶解无机氮、溶解无机磷以及活性硅酸盐呈显著正相关,与盐度呈显著负相关;微型浮游植物生物量与真光层深度、铵盐和溶解无机磷呈显著正相关;微微型浮游植物生物量与真光层深度、铵盐和溶解无机磷呈显著正相关,与pH值呈显著负相关。影响浮游植物群落粒级结构的最重要的海洋环境因子是盐度、真光层深度和溶解无机磷,尤其是溶解无机磷浓度,其值一旦升高,浮游植物极有可能从磷胁迫状态发展为赤潮。控制排污、实时监控、增强预警能力和改善生态环境是控制和预防营养盐污染以及赤潮暴发的有效手段。     

春季西沙永乐龙洞浮游植物的昼夜垂直分布特征

南海永乐龙洞位于西沙群岛永乐环礁,是迄今为止发现的最深的海洋蓝洞,水文环境及理化因素特殊,90 m以下水体为无氧环境。为研究永乐龙洞浮游植物的群落组成及其昼夜变化,于2017年3月在龙洞、潟湖及外礁坡进行浮游植物样品采集。研究结果表明:龙洞内叶绿素a浓度呈现随深度先增大后减小的趋势,日间浓度最大值层出现在40 m层(0.42μg/L),夜间则出现在20 m层(0.59μg/L)。永乐龙洞微微型浮游植物丰度介于1.1×10^3~5.1×10^4 cells/mL。聚球藻在上层水体占优势(0~20 m),40 m以下水层原绿球藻丰度对微微型浮游植物丰度贡献率最大(90%以上),微微型真核浮游植物丰度在整个水体都较低(除20 m层)。微微型浮游植物昼夜存在明显差异,夜间其丰度最大值层为20 m层,日间则上移至表层。本研究共记录微型和小型浮游植物5门41属55种(含未定种)。其中,硅藻门25属34种、甲藻门12属15种、金藻门1属1种、蓝藻3属、隐藻1属。微型和小型浮游植物丰度介于3.3×10^2~9.8×10^4 cells/L。甲藻丰度对浮游植物总丰度贡献率最大,其次是硅藻,隐藻和蓝藻丰度仅在少数水层占优势。微型和小型浮游植物昼夜变化明显,夜间丰度最大值层为20 m层,日间则出现在40 m层。微微型、微型和小型浮游植物垂直分布与叶绿素a浓度垂直分布一致性高。龙洞浮游植物的种类数和丰度高于潟湖和外礁坡。     

营养盐和重金属等环境因子对莱州湾浮游植物的影响

莱州湾是渤海典型海湾,沿岸工业区的存在产生大量营养盐和重金属等物质随黄河、小清河等多条河流汇入莱州湾,对浮游植物生长产生影响。本文于2020年8月对莱州湾开展海洋调查工作,研究了营养盐、重金属等环境因子的分布情况,及其对莱州湾浮游植物生长的影响。结果表明：沿岸黄河、小清河等带来的径流输入,导致黄河入海口(站位L1的E=1.25)和莱州湾西南部海域(站位L5、20的E值分别为16.96、1.14)呈现富营养化,整个调查海域约95%的站位存在磷的潜在限制;对应富营养化海域为浮游植物生物量高值区,有更高比例的甲藻物种数和赤潮种(中肋骨条藻、夜光藻和海洋原甲藻);重金属(铅和砷)处于理论上可能抑制浮游植物生长的浓度,但高营养负荷可能掩盖了重金属毒性,导致浮游植物生物量与重金属铅和砷呈现正相关。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Assessing the Validity of Seismo-Acoustic Predictor Equations for Obtaining Seabed and Sub-Surface Sediment Physical Properties

The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.     

A Review of Some Environmental Issues Affecting Marine Mining

This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested.     

Increased Particle Fluxes at the INDEX Site Attributable to Simulated Benthic Disturbance

Particle fluxes were measured 7 m above the sea bottom during the predisturbance, disturbance, and postdisturbance periods by using time series sediment traps attached to seven deep-sea moorings deployed in the INDEX experiment site in the Central Indian Basin. The predisturbance particle fluxes varied between 22.3 to 55.1 mg m -2 day -1 . Increased and variable particle fluxes were recorded by the sediment traps during the disturbance period. The increase observed was 0.5 to 4 times more than the background predisturbance fluxes. The increases in particle fluxes (~4 times) recorded by the sediment trap located in the southwestern direction (DMS-1) were the greatest, which could be the result of preferential movement of resuspended particles generated during the deep-sea benthic disturbance along the general current direction prevailing in this area during the experimental period. Also, the traps located closer to the disturbance area recorded greater fluxes than did the traps far away, across the Deep Sea Sediment Resuspension System path. This variability in recorded particle fluxes by the traps around the disturbance area clearly indicates that physical characteristics such as grain size and density of the resuspended particles produced during the disturbance had an important effect on particle movement. The postdisturbance measurements during ~5 days showed a reduction in particle fluxes of ~50%, indicating rapid particle settlement.     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Determination of In Situ Sediment Shear Strength from Advanced Piston Corer Pullout Forces

The advanced piston corer (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments.     

Interannual Sea Level Variations and Annual Tides in the Northwestern Pacific

Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.     

Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects

The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.