山东半岛南部近岸海域富营养化状况的多元评价研究

于2006年至2007年分四个季度月对山东半岛南部近岸海域(35.5°~36.7°N,119.8°~121.3°E)的营养盐和叶绿素a进行了设站调查,分析了该海域上述因子时空分布特征,同时应用主成分分析(PCA)和潜在富营养化评价方法对该海域的富营养化状况进行了分析,初步确定了影响该海域富营养化状况的主要驱动因子。结果表明:(1)在时间分布上,总氮、总磷、活性磷酸盐、溶解态氮与总无机氮和活性硅酸盐的季节变化类似,均表现出秋冬季浓度较高而春夏季较低的趋势,而总磷、溶解态磷的浓度则在冬季最高,在夏秋季较低,叶绿素a浓度的季节变化趋势为春夏季较高,而冬季最低;该海域营养盐与叶绿素a浓度空间分布以胶州湾和丁字湾为中心,总体呈现出由近岸向离岸海域逐渐递减的趋势;(2)应用主成分分析对调查结果进行分析,结果显示10项调查指标(营养盐与叶绿素a)可以转换提取4个主成分,解释82.00%的结果。主成分综合得分可以作为富营养化程度的评价指标,据此得到调查海域富营养化状况的季节变化趋势由高到低依次为秋季、冬季、春季、夏季,总体呈现出以胶州湾和丁字湾为中心,沿岸及北部海域富营养化程度较高的格局;分析结果还表明总无机氮是该海区富营养化形成的主要驱动因子;(3)利用潜在富营养化评价方法对调查海域富营养化状况进行了初步分析,结果表明该海区仅于秋季表现为总体中度营养,在其他季节总体趋势均为贫营养,胶州湾与丁字湾附近海域在春秋季表现为磷限制潜在富营养化。主成分分析法较潜在富营养化评价方法更敏感,不仅能够定量表述海区富营养化状况,而且能够有效确定富营养化驱动因子。     

中国近岸海域潜在性富营养化程度的评价

根据近岸海域的富营养化普遍受营养盐限制的特征,提出了潜在性经的概念,并在此 提出了一种新的富营养化分级标准及相应的评价模式,运用该模式分别评价了厦门海域及中国近海的富养化程度。结果表明,厦门东海域敞 于贫营养水平,而西海域则为磷限制潜在性营养水平;与厦门西海域相近,中国近海主要河口、海湾总体上处于磷限制或磷中等限制潜在性富营养水平。     

应用压力-状态-响应模型评价象山港富营养化水平

象山港因其特殊的地理环境及功能特性, 富营养化问题突出, 以往以营养盐为指标的评价不能有效反映象山港的实际情况, 因此亟须对其富营养化水平开展客观、准确的评价和研究。文章利用2001年—2015年间象山港海域监测数据, 根据我国水体富营养化研究现状与评价标准, 结合象山港实际情况, 对部分指标进行改进, 构建了新的压力-状态-响应模型。应用该评价模型, 研究了象山港水体富营养化压力程度, 建立了以叶绿素a为初级症状指标, 以底层溶解氧和赤潮发生情况为次级症状指标的富营养化状态等级。同时, 结合象山港实施海域海岸带整治、五水共治、污染物总量控制及减排等因素预测了富营养化响应状况。结果显示, 在“优、良、中、差、劣”五个富营养化等级中, 象山港综合评价等级为中等富营养化程度。其中, 压力、状态、响应的评价等级均为中级。通过预测, 未来几年象山港富营养化综合评价等级也基本维持在中等水平。该评价结果较好地反映了象山港水体富营养化压力和症状水平, 其结论更符合实际, 有助于政府部门对海域富营养化进行科学和有针对性的管理。     

基于熵最大化的富营养化二级模糊评价模型——以长江口及邻近海域富营养化评价为例

为进一步丰富符合中国国情的多参数水质富营养化综合评价方法体系,本文在确立富营养化评价指标体系的基础上,通过对不同富营养化模糊判别方法的比较,从富营养化症状和近海生态系统的响应角度出发,确定并建立了"致害因素-效应"框架下的基于熵最大化原理的二级模糊评判方法。不同评价方法对中国典型河口——长江口及邻近海域富营养化程度的评价结果显示,基于熵最大化原理的二级模糊评价模型所得结果最符合以逼近理想解排序所得的合理营养等级,并且在对各种方法所得评价结果进行理想解的接近度以及广义距离排序时,基于熵最大化的二级评价模型均排在较前的位置,可知该模型的评价结果最接近研究海域水环境系统各指标的实际情况,具有一定的可行性。     

基于PSR模型的珠江口海域富营养化特征与评价

本研究依据2020年4月（春季）和2020年10月（秋季）珠江口海域的调查数据,根据“压力-状态-响应”（PSR）模型对珠江口海域进行富营养化状况评价,并通过主成分分析以及相关性分析来说明各环境因子对珠江口富营养化的影响。结果表明：在“优、良、中、差、劣”5个富营养化等级中,珠江口PSR综合评价等级为“差”等级,近几年内变化趋于稳定。有机污染物与营养盐对珠江口富营养化的影响较大,其次为叶绿素a和溶解氧。珠江口营养盐与盐度呈显著负相关,与叶绿素a呈显著正相关。周边城市的陆源排放输入是导致珠江口富营养化的主要因素,河口稀释混合作用以及浮游植物对珠江口的富营养化程度有影响。     

厦门海域水体富营养化状况综合评价

以2003—2012年厦门海域监测数据为基础,参考美国第二代富营养化评价方法,针对厦门海域实际监测特点并结合我国水体富营养化研究现状与评价标准,进行部分改良,使其更适用于该海域水体的富营养化状况评价。应用此评价体系,本文研究了厦门海域水体富营养化压力程度;以叶绿素a为初级症状指标、以底层溶解氧及赤潮发生状况为次级症状指标分析了水体富营养化的状态水平;并通过结合厦门海域整治、人口增长趋势、经济发展趋势以及周边营养盐入海现状等因素预测了未来厦门海域水体富营养化压力变化趋势。结果表明,厦门海域水体综合评价等级为中等富营养化程度,其中压力等级为中高、状态和响应的评价等级均为中级。该评价结果较好地反映了厦门海域水体富营养化程度、压力和症状,便于海洋管理部门对厦门海域环境容量进行监控与管理。     

基于人工神经网络的夏季辽东湾北部河口区富营养化评价

为掌握辽东湾北部海域营养盐污染状况及富营养化水平,本文基于2014—2016年夏季现场调查资料,分析了该海域无机氮、活性磷酸盐、化学需氧量和叶绿素a的时空分布特征,并建立误差逆向传播算法(Back Propagation)的人工神经网络模型对该海域富营养化水平进行评价。结果表明:受2015年夏季辽宁省干旱和2016年夏季东北地区大规模降雨的影响,辽东湾北部海域营养盐、化学需氧量和叶绿素a浓度均呈现2014年较高,2015年降低,2016年又上升的趋势。调查海域营养盐结构在2014年和2015年主要表现为磷限制,2016年调查海域北部为磷限制,南部为氮限制。BP人工神经网络的评价结果表明,调查海域夏季富营养化水平呈现2014年较为严重,2015年降低,2016年又升高的"U"型特征。富营养化程度严重的区域主要出现在大辽河口、辽河口及其近岸海域。大辽河口富营养化程度在3年夏季均保持较高水平,其中2014年和2016年与辽河口富营养化水平相当,而2015年远高于辽东湾北部其他海域。BP人工神经网络在进行富营养化评价过程中,能够综合考虑各评价指标对海水富营养化的贡献率,避免对营养盐的过度依赖,同时减少评价过程中的主观误差,是一种更加客观合理的富营养化评价方法。     

近岸生态环境质量综合评价方法及其应用

在借鉴国外近岸海域生态环境质量综合评价方法的基础上,结合我国近海的生态环境特点和现有的海洋环境质量评价标准及资料状况,提出了一种适合我国近海特点的生态环境质量综合评价方法.该方法选取了生物学质量要素、水质要素、沉积物质量要素和生物体质量要素共4类评价指标,根据评价标准对各类指标进行赋分,最后集合4类评价指标得分,将近岸海域生态环境质量划分为"优、良、中、差、劣"共5个最终级别.将此方法应用于胶州湾海域,结果表明20世纪九十年代以来胶州湾生态环境质量基本维持在"良/中"级水平,且逐渐趋于好转.通过实际应用,证实了该方法具有一定的科学性和较好的可操作性.     

我国近岸海域富营养化评价新方法及应用

参考目前国际上被广泛应用的第2代富营养化评价方法体系,结合我国近岸海域生态环境特点和监测资料现状,建立了一种以压力-状态-响应模式为基本框架、以富营养化症状为主的第2代近岸海域富营养化评价体系。实际应用结果表明,应用本方法得出的长江口海域富营养化状况评价结论,与目前国外主流评价方法的评价结论一致,而且本方法具有评价指标代表性强、数据资料易得、评价标准有据、方法简便易行等优点。     

我国近岸海域富营养化评价新方法及应用

参考目前国际上被广泛应用的第2代富营养化评价方法体系,结合我国近岸海域生态环境特点和监测资料现状,建立了一种以压力-状态-响应模式为基本框架、以富营养化症状为主的第2代近岸海域富营养化评价体系。实际应用结果表明,应用本方法得出的长江口海域富营养化状况评价结论,与目前国外主流评价方法的评价结论一致,而且本方法具有评价指标代表性强、数据资料易得、评价标准有据、方法简便易行等优点。     

Microbial Biomass and Organic Nutrients in the Deep-Sea Sediments of the Central Indian Ocean Basin

In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance.     

Assessing the Validity of Seismo-Acoustic Predictor Equations for Obtaining Seabed and Sub-Surface Sediment Physical Properties

The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths.     

海上大直径钢管桩打桩过程中桩周土强度弱化研究

海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。     

Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification

An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm.     

Interannual Sea Level Variations and Annual Tides in the Northwestern Pacific

Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone.     

Deep-Sea Sediment Compression Curves: Some Controlling Factors, Spurious Overconsolidation, Predictions, and Geophysical Reproduction

Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations.     

Flow Cytometric Analysis of Coastal Lagoon Bacterioplankton and Picophytoplankton: Fixation and Storage Effects

The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics.     

Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans

A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level.